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백문기,배양섭,홍한기 한국곤충학회 1999 Entomological Research Vol.29 No.4
한국산 야생쥐류에 기생하는 이류의 숙주 특이성에 관하여 1989년 3월부터 1994년 8월까지 8도 7시 26군 38지역에서 실시한 결과, 한국산 야생쥐류에 기생하는 이류는 등줄쥐이 (굵은몸쥐이과), Hoplopleura acanthopus (굵은몸쥐이과), 생쥐이 (가는몸쥐이과), 집쥐이 (가는몸쥐이과), Mallophaga sp.의 2목 3과 5종으로 규명되었으며, 이 중 H. acanthopus는 대륙밭쥐, 집쥐이는 시궁쥐에 숙주 특이성을 나타냈고, 등줄쥐이와 생쥐이는 등줄쥐에 숙주 특이성을 나타냈다. Host Specificity of ectoparasitic lices of wild rodents in Korea was studied from March 1989 to August 1994. Five species belonging to three genera were collected from wild rodents during the survey and identified to be Hoplopleuridae: Hoplopleura affinis (Burmeister), H. acanthopus Ferris; Polyplacidae: Polyplax serrate (Burmeister), P. spinulosa (Burmeister); Mallophaga sp. The Specific rodent host species for the ectoparasitic lice were Eothenomys regulus for H. acanthopus, Rattus norvegicus for P. spinulosa, and Apodemus agrarius for H. affinis and P. serrata.
백문기,Deibler, G.E.,Martenson, R.E.,Kies, M.W. 충남대학교 의과대학 지역사회의학연구소 1980 충남의대잡지 Vol.7 No.2
S-adenosyl-L-methionine : protein arginine N-methyltransferase having an optimum pH around 7.2 has been purified approximately 1,800 fold with 16% yield from wheat germ, and an attempt was made to resolve controversial multiplicity on the enzyme. The end products of the purified enzyme preparation were N^G-monomethylarginine and N^G, N'^G-dimethylarginine in a ratio of 70 : 30, and the enzyme preparation lost the ability of less purified ones to carry out the synthesis of N^G, N^G-dimethylarginine, strongly indicating that different enzymes were responsible for the production of different methylated derivatives of arginine. Contrary to our expectation, histone was better substrate than myelin basic proteins from various sources. From the above results and products ratio change at each step of purification, we discussed the possibility that three enzymes were involved in the formation of three methylated arginine derivatives which has been identified so far.
Two New Records of Phycitine Moths (Lepidoptera, Pyralidae) in Korea
백문기,차진열,배양섭 한국곤충학회 2002 Entomological Research Vol.32 No.2
Two species, Assara inouei Yamanaka and Assara pallidella Yamanaka, are newly added to the pyralid fauna of Korea, with redescriptions and illustrations of adult and genitalia.
태반조직의 Nucleases 에 관한 연구 ( 1 ) 사람 태반조직의 Deoxyribonucleases
백문기,황병두,금기창 ( Moon Kee Paik,Byung Doo Hwang,Ki Chang Kum ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.1
The properties of acid deoxyribonuclease purified from human placenta have been investigated, and serial estimation of acid and alkaline deoxyribonuclease in human placenta has been performed. The acid deoxyribonuclease is partially purified forty-fold with specific activity of 240 units/㎎ protein. The acid deoxyribonuclease having an optimal pH around 5 is heat-labile with complete loss of activity at 70 for 10 minutes. The acid deoxyribonuclease is inhibited by Mg^(2+), Mn^(2+), Car and Co^(2+) above 1 mM concentration, suggesting that the enzyme is not dependent on divalent cations. The acid deoxyribonuclease acts on both native and heat-denatured DNA with preference for the former. A gradual decrease in the acid deoxyribonuclease activity occurs with advancing gestation after 20 weeks, the activity at term being about one-fourth of that before 20 weeks. The alkaline deoxyribonuclease activity is maintained untill approximately the 16th week of gestation at which the functioning villi has been established, and thereafter decreases rapidly with no apparent activity about 28 weeks. From the activity changes with gestational age, it is discussed that the acid deoxyribonuclease is related to DNA synthesis of the placenta whereas the alkaline deoxyribonuclease plays an important role in the formation of the functioning villi, suggesting that alkaline deoxyribonuclease is a useful enzyme to evaluate the function of the early placenta.
한국 고유종 Pisidium (Neopisidium)coreanum (산골조개)의 metallothionein 유전자를 기초로 한 분자계통 분류학적 연구
백문기,이준서,강세원,이재봉,강현정,조용훈,노미영,한연수,최상행,채성화,박홍석,이준상,이용석 한국패류학회 2009 The Korean Journal of Malacology Vol.25 No.2
Pisidium (Neopisidium) coreanum is a freshwater snail and lives in spring water near mountain areas. Interestingly, this snail has traditionally regarded as medicinal food, and thus has been used as folk remedies for healing broken bones. Recently, alpha classification on Pisidium (Neopisidium) coreanum through re-description has been conducted. However, not much attention has so far been made in beta classification. In this study, we performed the beta classification based on metallothionein (MT) genes found from various organisms. To this end, the complete cDNA sequences were obtained from the Expressed Sequence Tag (EST) sequencing project of Pisidium (Neopisidium) coreanum. The coding region (315 bp) gives an amino acid sequence of 105 residues. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Pc-MT gene indicate that Pisidium (Neopisidium) coreanum has similarity to freshwater bivalve such as Dreissena polymorpha (zebra mussel), Unio tumidus (swollen river mussel) and Crassostrea ariakensis (suminoe oyster). Pisidium (Neopisidium) coreanum is a freshwater snail and lives in spring water near mountain areas. Interestingly, this snail has traditionally regarded as medicinal food, and thus has been used as folk remedies for healing broken bones. Recently, alpha classification on Pisidium (Neopisidium) coreanum through re-description has been conducted. However, not much attention has so far been made in beta classification. In this study, we performed the beta classification based on metallothionein (MT) genes found from various organisms. To this end, the complete cDNA sequences were obtained from the Expressed Sequence Tag (EST) sequencing project of Pisidium (Neopisidium) coreanum. The coding region (315 bp) gives an amino acid sequence of 105 residues. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Pc-MT gene indicate that Pisidium (Neopisidium) coreanum has similarity to freshwater bivalve such as Dreissena polymorpha (zebra mussel), Unio tumidus (swollen river mussel) and Crassostrea ariakensis (suminoe oyster).
初期 胎盤組織의 膜結合 Deoxyribonucleases의 性狀
白汶基,黃炳斗,琴基昌 충남대학교 의과대학 지역사회의학연구소 1977 충남의대잡지 Vol.4 No.2
Some properties of the plasma membrane-bound deoxyribonucleases (DNases) from the early placenta have been investigated. Evidence is indicating that the DNases having an optimal pH around 8 (pH-8-DNase) and 9.5 (pH-9.5-DNase) are distinct enzyme entities. The pH-8-DNase and the pH-9.5-DNase are required Mg^2+ and Co^2+, respectively, for its maximal activivity. The above DNases are heat-labile with some difference and act both on the native and the heat-denatured DNA with preference for the latter. Lipase, phospholipase-C and -D are not successful for the solubilization of the pH--9. 5-DNase from the membrane structure of the early human placenta. The appararent activity of the pH9.5-DNase is not measureable in the normal term placenta having no cytotrophoblast. From the above results and the fact that the DNA is not only associated with the membrane structure, but als othe membrane-DNA interaction may be relevant to several pathologic process, including aging and carcinogenesis, we discussed that the pH-9.5-DNase is related to the differenciation of the early placenta.
白汶基,黃炳斗,李正馥,琴基昌 충남대학교 의과대학 지역사회의학연구소 1978 충남의대잡지 Vol.5 No.1
Properties of alkaline deoxyribonucleases from cytosol and membane fractions of the early human placenta have been investigated. Evidence is indicating that the cytosol deoxyribonuclease (DNase) having an optimal pH around 9 and the plasma membrane bound DNases having an optimal pH around 8 (pH- 8-PM-DNase) and 9. 5 (pH-9.5-PM-DNase), respectively, are distinct enzyme entities. The cytosol DNase, partially purified, is activated by 1mM CO^2+ and the pH-8-PMDNase and the pH-9. 5-PM-DNase are required Mg^2+ and Co2^2+, respectively, for its maximal activity. The cytosol DNase is relatively heat-labile, whereas the plasma membrane bound DNases are heat-labile with some differnce. The above DNases act both on the native DNA and the heat-denatured DNA with preference for the latter. Kinetic analysis shows that the maximal velocity is independent of Co^2+, while Km decreases from 4. 16 × 10 exp(-4) M DNA-p in the absence of Cot^2+ to 9. 4×10 exp(-5) M DNA-p in the presence of Co^2+. From the above result and the fact that the DNA is not only associated with membrane structure, but also membrane-DNA interaction may be relevant to several pathologic process, including aging and carcinogenesis, we discussed that the PH-9-PM-DNase is related to the differenciation of the early placenta.