천마 성분인 4-히드록시-메톡시벤즈알데히드 및 파라-히드록시벤즈알데히드의 흰쥐에서의 약물동태

용철순,권기철,김정애,하정희,이동웅,허근 영남대학교 약품개발연구소 1999 영남대학교 약품개발연구소 연구업적집 Vol.9 No.-

Gastrodia elata (GE) is an oriental medicinal herb which has been used traditionally for the treatment of var-ious brain diseases including convulsion and epilepsy. The purpose of this study was to determine pharmacokinetic param-eters of 4-hydroxy-3-methoxybenzaldehyde (HMBA) and p-hydroxybenzaldehyde (PHBA), constituents of GE, in rats. Male rats were cannulated in the femoral vein, femoral artery, bile duct and ureter. They received a single i.v. bolus dose of either HMBA or PHBA through the femoral vein. The concentration of HMBA or PHBA in plasma, bile and urine sam-ples were analyzed by reversed-phase HPLC. HMBA and PHBA have very short half-lives, i.e. 4.03 and 2.26 minutes respectively. Most of HMBA and PHBA were thought to be eliminated through metabolism as the metabolized fraction approaches unity. Derivatives of HMBA or PHBA with longer biological half-lives should be designed to develop better anticonvulsants and more complete qualitative and quantitative understanding of the overall pharmacokinetic fate of these compounds awaits further investigation.

Bi_2(Mo O_4)_3 단결정의 광학적 성질

李丁柱,金仁湖,姜光鏞,張敏守 慶尙大學校 기초과학연구소 1988 基礎科學硏究所報 Vol.4 No.-

Bi_2(M_0O_4)_3 single crystal was grown by Czochralski technique. From the UV-VIS spectrum and the IR spectra Bi_2(M_0O_4)_3crystal was turned out having a indirect absorption process with energy gap, 2.8eV and having complicate absorption edge with maximum value in vicinity of 1860cm^-1. According to the crystallographic point group(2/m) and monoclinic system,Bi_2(M_0O_4)_3 crystal has two Raman active modes(Ag, Bg mode). Thus, the evolution of Raman spectra with temperature corresponding to selected experimental configuration-x(zz)y (Ag-mode), x(yx)y(Bg-mode)-was observed. From photoluminescence experiment, a violet-fluorescent light was observed. But we had a difficulty in analyzing the broad -spectra detected actually.

In situ 고상 에피택시 방법에 의한 CoSi₂/Si(111)구조의 형성

이정주,강민성,김현수,최치규,이운환,이종덕,이영백,김건호,이정용 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

초고진공에서 공상에피택시(solid-phase epiyaxy, SPE) 방법으로 CoSi₂를 Si(111)기판 위에 에피택시 성장시켰다. 2MeV⁴He++ ion후방산란 분광기와 사중결정 x-선 회절기 및 고분해 투과전자 현미경을 이용하여 성장된 CoSi₂의 상과 조성, 결정성, 그리고 계면의 미시구조를 조사하였다. 초고진공상태의 실온에서 Si(111)-7X7기판에 Co를 약 20 ~50 ?? 중착한 후 SPE에 의하여 실리사이드를 형성시키는 경우 600℃에서는 B-형의 CoSi₂가, 그리고 700℃에서는 A형의 CoSi₂가 선택적으로 에피택시 성장하였으며, 정합성은 B-CoSi₂[110]//Si[110] and CoSi₂(111)//Si(111)이, 그리고 700℃에서는 A-CoSi₂[110]//Si[110], CoSi₂(002)//Si(002)을 나타내었다. Epitaxial films of CoSi₂were grown on Si(111) substrates by in situ solid-phase epitaxy in a ultrahigh vacuum. The phase, the chemical composition, the crystallinity, and the microstructure of the Silicide/Si interface were investigated by 2-MeV⁴He++ ion-backscattering spectrometry, quadruple crystal X-ray diffractometry, and high-resolution transmission eletron micorscopy. High-quality films of either B-type or A-type CoSi₂ could be grown selectively on Si(111) substrates by depositing ~ 20 ~ 50 ?? of Co on a Si(111)-7X7 substrate followed by in situ annealing at 600℃ or 700℃ for 10 min. The matching face relationships are CoSi₂[110]//Si[110] and CoSi₂(111)//Si(111) by B-type CoSi₂ and CoSi₂[110]//Si[110]//Si[110] and CoSi₂(002)//Si(002) for A-type CoSi₂.

위 윤상근 세포의 Ca^2+-의존성 K^+-전류에 미치는 Ascorbate의 효과

배정원,이무열,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.2

In order to observe the effect of ascorbate on the Ca^2+-activated K^+ current in pyloric circular muscle cells which plays a critical role in generation and maintenance of repolarization and automatism, apart of pyloric circular muscle tissue was excised out, digested in collagenase containing Ca^2+-free physiological solution to get single circular muscle cells and the whole cell mode of patch-clamp experiment was conducted. Following results were obtained : 1. Oscillatory outward current was reduced by ascorbate in case of high intrapipette Ca^2+ concentration in whole voltage range and was enhanced in case of low intrapipette Ca^2+ concentration. 2. Oscillatory outward current was reduced in Ca^2+-free physiological solution with calcium channel blocker and not effected by ascorbate. 3. There were no qualitative differences among the control and ascorbate-treated group in effects of pH, steady state inactivation and the remaining component of the outward current. The above results could be concluded that ascorbate effects calcium-dependent component of the oscillatory outward current in the pyloric circular smooth muscle cells.

한국인 젊은이의 혈중 아연 농도

김정용,정규철 중앙대학교 의과대학 의과학연구소 1986 中央醫大誌 Vol.11 No.1

Zinc concentrations in whole blood were measured with 145 healthy young adults, 102 male and 43 female volunteers aged from 16 to 25 years, who had no obvious exposure to zinc previously. Analysis of zinc was made by a simple one-step dilution method using a flame atomic absorption spectrophotometer(Model A5100, Baird Ltd.). As mean recovery rates of zinc added to the samples ranged from 57 4 to 101.O% with coefficients of variation ranged from 1.6 to 4.5%, the analytic method was proven to be reliable with high precision. Zinc concentrations in whole blood showed the normal frequency distribution unlike lead or mercury in blood which showed the log-normal distribution. Mean concentration of zinc was 6.48±1.04 ㎍/ml, and showed not only inter-individual variation (p<0.01) but also differed between both sexes (p<0.01) and geographic locations(p<0.01) in which the subjects had been grown up.

천마의 GABA-benzodiazepine 수용체 복합체에 대한 조절작용

하정희,이동웅,어경윤,하정상,김현주,용철순,허근 영남대학교 약품개발연구소 1998 영남대학교 약품개발연구소 연구업적집 Vol.8 No.-

Methanol extract of G. elata inhibited the binding of [³H]Ro15-1788, a selective benzodiazepine receptor antagonist, to benzodiazepine receptor of rat cortices. Saturation experiments followed by Scatchard analysis of the results showed that the inhibition of [³H]Ro15-1788 binding by G. elata. appeared to be competitive. These competitive inhibiton of the butanol fraction was observed to be higher than the methanol extract. Methanol extract of G. elata inhibited a [³H]flunitrazepam, a selective benzodiazepine receptor agonist, binding to benzodiazepine receptor. GABA significantly enhanced the inhibition of [³H]flunitrazepam binding by G. elata, and these "positive GABA shift" supported the strong possibility of agonistic activity to benzodiazepine receptor. Butanol fraction was observed to be higher than crude extract by methanol in an agonistic activity to benzodiazepine receptor, furthermore enhanced the binding of [³H]SR95531 to GABA_(A) receptor. Butanol fraction of G. elata significantly diminished the pentylenetetrazole-induced lethality of mice. From these results, it can be concluded that substance or substances with neurochemical properties characteristic of a benzodiazepine receptor agonist may be important components, and contribute to the anticonvulsant property of G. elata.

전이금속과 질소 - 산소를 가진 거대고리 리간드와의 착물형성에 관한 연구

최규성,김정,김용규 慶南大學校 附設 基礎科學硏究所 1992 硏究論文集 Vol.4 No.-

The stability constants of Co(II), Ni(II), Cu(II) ions with macrocyclic ligands containing nitrogen and oxygen donors such as 1, 15-diaza-3, 4:12, 13-dibenzo-5, 8, 11-trioxacycloheptadecane(NEOD), 1, 12, 15-triaza-3, 4 : 9, 10-dibenzo-5, 8-dioxacycloheptadecane(NDOE) have been determined by UV-VIS spectrophotometric method in DMSO. The order of complex stability constants of the transition metal ions with ligands is Co(II) < Ni(II) < Cu(II), the same as the natural order of stability published by Williams-Irving, and stability constants have been found to be effected by the chelate effect of macrocyclic ligands, so the order of ligands effect is NEOD < NDOE.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

열화상카메라를 이용한 LCD-TV의 열분포 분석 및 방열 성능 향상을 위한 연구

김윤석,김용재,이정권,정두환,고한서 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

In these days, demand of a LCD-TV is remarkably increasing with development of the LCD technology. However, there are thermal problems for improvement of efficiecy for the LCD-TV. Thus, this research analyzed thermal problems such as convection and conduction heat transfer characteristics in the LCD-TV using an infrared (IR) camera. The results using the IR camera have been compared with those using T-type thermocouples. The results of the experiment showed that; 1. surface temperatures of inverter modules, CCFL and electrodes were higher than any other parts in the LCD-TV. 2. temperature distributions of the LCD-TV appeared asymmetrically based on the center line. 3. results of the two testing equipments agreed well.

스포츠 매니지먼트활동에서 매니저들의 책임 수행에 관한 연구

박준석,김정묵,정석현,조영웅,이영성 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

Sport has taken a position as significant influence all over the world for a long time. These days, sport must exist with us in order to provide pleasure, so people have a tendency to expect it what happens to them. Therefore, a number of sport teams and competitions are able to stimulate people to be interested in sport. Sport event, information, equipment, and sport player management need systems which accompany with security and pleasure because the systematical managements establish security, and help sport players not to make an error that is a fountain of good quality game. Thus, sport players improve their ability, so the audience are willing to pay to see the game. In order to extend the power of sport influence, sport should be organized in terms of managing sport processes well. Unfortunately, the system of management for sport is not still adequate and organized in Korea. In other words, many materials must be sporadic, and newspapers or magazines have treated of sport management with fragmentary knowledge so far. Today, systematical management is essential to all segments of the sport industry, professional or amateur, school or club, private or government at any level community or individual. Hopefully, this research will be instructive as a starting point to derive a special quality of sport management which is suited to korea sport market by comparing to other management systems in order to formulate organized management industry.