실험적 진폐증에서 투여약제에 따른 면역학적 병리학적 조직변화

윤임중,임현우,노영만,오상용,정장영,임영,김경아 가톨릭 대학 산업의학 쎈타 산업의학연구소 1993 韓國의 産業醫學 Vol.32 No.2

To access the effects of some drugs such as piperazine phosphare, azathioprine and allopurinol in the experimental pneumoconiosis, 263 rats weighted 240-320 gm was divided into the control and eight experimental groups. To each group, the turbid solution mixed free silica, talc and natural coal dust of 50 mg in 0.8 ml saline were instilled intratracheally. At the begining and 5th week of experiment, piperazine phosphate(8mg/week orally), aspirin(1.5mg/day intramuscularly), azathioprine(25mg/day orally) and allopurinol(1.5mg/day orally) were administered simultaneously, while the dust turbid solution was only given to the control group. For each group the change of body weight. dry right lung weight, cellularity including total cell, macrophage, lymphocyte and neurtrophil in bronchoalveolar lavage fluid, superoxide ion, hydroxyproline, leukotriene B₄, tumor necrotsis factor, prostaglandin E₂were examined, and observed the pathological chages in lung tissue. The results were as follow : 1. The larger amount of their instilled dust and the longer observation period, the severer pathological findings were found in the lung tissue of each experimental group. 2. The body and dry right lung wight was not significantly changed in the experimental groups compared with the control group. 3. The number of total cell in bronchoalveolar lavage fluid was markedly less in the experimental group than the control one, while no significant difference was found in the percent alveolar macrophages and lymphocytes in both groups. However, the percent of neutrophiles was usually lower than in the control group. 4. The amount of superoxide ion was frequently less in the experimental group compared with the control one. 5. The amount of hydroxyproline was significantly lower in the experimental group than in the control one. 6. The amount of leukotriene B₄was usually less in the drug administered group than in the control one. 7. The amount of tumor necrotic factor was also frequently lower in each experimental group than in the control one. 8. The amount of prostaglandin E₂was usually higher in each experimental group than in the control one.

세무회계관련 학과 학사학위전공심화과정 개설 및 활성화 방안에 관한 연구

윤우영 ( Yun Woo-young ) 한국세무회계학회 2018 세무회계연구 Vol.0 No.56

최근 4차 산업혁명과 인구구조의 변화 등으로 전문대학 세무회계관련 학과는 새로운 교육방식으로의 전환과 전공분야에서 직무수행능력을 향상시킬 수 있는 계속교육의 필요성이 높아지고 있다. 본 연구에서는 재학생, 산업체, 졸업생을 대상으로 세무회계관련 학과의 학사학위전공심화과정의 개설 및 활성화 방안에 대해 설문조사를 활용하여 연구하였다. 본 연구의 분석 결과 첫째, 재학생들과의 효과적인 진로상담 및 진학상담 등을 통해 학사학위전공심화과정에 대한 인지도를 높일 필요성이 있으며, 둘째, 학사학위전공심화과정의 인재상은 현장기술활용 능력으로 설정해야 하고, 셋째, 회계직무와 세무직무에서 전문대학과 연계하여 4년제 대학과의 차별화된 직무능력향상을 위한 현장중심 교육과정을 개발·운영해야 한다. 마지막으로 학사학위전공심화과정의 적절한 수업시간대로 평일야간을 선호하는 것으로 나타났다. 향후 세무회계관련 학과의 새로운 발전 방안과 직업교육·훈련의 수직적 연계 방안에 관한 후속연구가 활발히 진행되기를 기대한다. Recently, due to the 4th industrial revolution as well as changes in population structure, the need for a new way of learning together with continuing education in order to improve their job performance in major fields is increasing in the departments related to tax & accounting. In this study, we studied how to open and activate the advanced course to confer bachelor’s degree for students, industry, and graduates related to tax & accounting departments. First, There is a need to raise awareness of the advanced course to confer bachelor’s Degree through effective career counseling and consultation with students, second, the advanced course to confer bachelor’s degree should be focusing on the hands-on capability to use skills on the job, third, it is necessary to develop and run the field-oriented curriculum for improving the accounting & tax job ability working together with 2 year colleges, which can be differentiated from the four-year university, finally, weekday nights turned out to be the proper class time for the advanced course to confer bachelor’s degree. Further active research on new ideas for development of tax accounting departments as well as the vertical linkage of vocational education and training is expected.

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

몇가지 植物의 棲息地 造成에 關한 硏究

윤종선,김익환,신세균,심우경,이철희,신영철 韓國植物ㆍ人間ㆍ環境學會 1999 인간식물환경학회지 Vol.2 No.2

종의 棲息密度가 낮아지고 있는 하늘나리, 섬초롱, 자란 3종의 식물을 인위적으로 棲息地를 造成하기 위하여 국립공원 속리산 등산로변 標高 200m, 400m, 600m 지점에 150주식 종당 450주를 조직배양 및 분주에 의하여 육묘 된 묘를 이식하여 생존상태 및 棲息地 造成의 가능성을 2년에 걸쳐 검토한 결과는 다음과 같다. 1. 造成 棲息地의 토양의 理化學的 造成은 pH 5.2∼5.6, 유기물 함량 29∼54g/㎏, CEC 9.4∼11.5㎝ mol(+)/㎏으로 산성을 띠고 있었으며, 유기물 함량은 높은 편이었고, 염기치환용량은 낮았으나 비교적 우리나라 典型的 山地土壤의 理化學的 造成과 유사하였다. 2. 棲息地內 생장기간의 投光率은 標高 200m 지역은 10.8∼11.6%, 400m lw역은 10.8∼11.4%, 600m 지역은 11.0∼12.8%로 식재식물의 生長環境에 부족한 상태였다. 3. 식재 식물 수는 식재 후 활착율은 3종 모두 양호하였으나, 점차 감소되었으며, 특히 자란의 감소율이 높았다. 감소의 원인을 분석한 결과 하늘나리, 섬초롱, 자란 모두 굴취나 절취의 피해가 많았으며, 특히 자란은 凍害에 의한 枯死가 1년차 72∼86주, 2년 차에서 19∼35주로 조사도어 자연상태에서의 越冬은 어려운 것으로 나타났으며, 집중호우에 의한 流失도 감소의 한 원인이었다. 4. 식재식물의 생장상태는 불량하였는데 이는 投光量 부족으로 판단되어 棲息地造成時에는 錄陰期 投光量에 대한 사전 검토가 중요한 요인으로 나타났다. 5. 개화율은 섬초롱과 자란에서 높았으며 標高가 높을수록 개화기는 2∼4일 늦어지고 휴면기는 빨라지는 경향이었다. 식물 棲息地 造成時에는 造成 식물의 생장에 적합한 토양, 光環境 등을 고려한 위치 선정이 중요하며, 上層林에 의한 遮光으로 下層 초본식물 遷移速度가 빨라짐으로써 光環境 개선을 위한 上層林의 間伐, 周邊樹種의 更新이 요구된다. In order to make the artificial habitats of Lillium concolor, Campanula takesimana and Bletilla striata that the number of plant became sparse, 150 plants per species were transplanted at the region of 200m, 400m and 600m above sea level. And the growth of plants and the potential as habitats creation were analyzed. In the physical and chemical properties of soils in the there, soil pH was 5.2 to 5.6, organic matter content was 29 to 54g/㎏ and cation exchange capacity(CEC) was 9.4 to 11.5㎝ mol(+)/㎏. The transmittance rates of sunlight of the habitats during the growing periods were 10.8 to 11.6% in the 200m region, 10.8 to 11.4% in the 400m region and 11.0 to 12.8% in the 600m region. These transmittance rates were deficient for normal growth of plants. After the plants were transplanted, the survival rates of three species were high in common but the rates decreased gradually. Especially the decrease rate was high in the Bletilla striata. The causes of decrease were digging and cutting by climbers, sweeping asay by rainfall and freezing damage. Especially, the cold tolerance of Bletilla striata was weak in the natural condition. The growth of planted plants was poor, and it was assumed that the reason was shortage of sunlight transmittance. The flowering rates were high in the Campanula takesimana and Bletilla striata. And as the elevations were high, the flowering was later by 2 to 5 days but the dormancy was earlier. In the artificial making of plant habitats, the growth environment of plants have to be considered to choose the suitable region. And, because the habitats are shaded by the trees and bushes, the thinning of trees and the change of species are needs to improve the light transmission.

냉기 치료에 따른 둔근에서의 피부 및 근육내 온도 변화

정우성,김미정,박시복,이상건,김영호,양길태,장윤희 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.2

공간인식에 공간깊이와 거리의 영향연구 : Depth(Space syntactic distance)와 Distance(물리적인 거리)를 중심으로

윤용우,김영욱,박영기 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(계획계)

The purpose of this study is to prove interrelationship between space syntax and mental map that enable us to analyze the general characteristics of spatial configuration and spatial cognition and to determine which one have more influence on spatial cognition between depth and distance. The result of this study were as follows; (1)there is strong relationship between spatial configuration and spatial cognition, (2)depth have stronger influence on recognizing spatial configuration than distance.

Cisplatin의 이독성 발견에 있어 초고주파 청각검사에 대한 연구

우훈영,김갑무,노윤성,김성록 인제대학교 1994 仁濟醫學 Vol.15 No.1

Cisplatin은 비뇨생식기 영역의 악성종양 및 두경부 편평상피암에 효과적인 항암제로 이에 의한 이독성은 주로 와우의 기저부에 영향을 미쳐 고음역 난청을 초래한다고 알려져 있다. 본 연구의 목적은 Cisplatin의 이독성을 발견하는데 있어 일반적인 순음청력검사와 비교하여 초고주파 청각검사의 유용성을 평가하는데 두었다. Cisplatin을 단독 혹은 타항암제와 병용하여 투여한 11명의 환자, 총 22귀를 대상으로 순음청각검사 및 초고주파 청각검사를 실시하여 다음과 같은 결과를 얻었다. 청각손실이 없었던 경우는 5귀(23%), 저주파 영역(125∼8,00Hz)의 청각손실이 있었던 경우는 17(5%), 초고주파 영역(10,000∼20,000Hz)의 청각손실이 있었던 경우는 67(27%), 저주파 및 초고주파 영역 모두에서 청각손실이 있었던 경우는 107(45%)였다. 결론적으로 Cisplatin에 의한 이독성을 발견하는데 있어 초고주파 청각검사가 순음청각검사보다 유용함을 알 수 있었다. Cis-diaminedichloroplatinum, or cisplatin, is an effective antineoplastic agent against genitourinary cancers and squamous cell carcinoma of head and neck. Ototoxicity of cisplatin is cochlear damage with a predilection for involvement of higher frequencies. The purpose of this study is to evaluate the usefulness of high frequecy audiometry in detection of cisplatin ototoxicity compared with conventional audiometry. The auditory function of 22 ears of 11 subjects receiving cisplatin alone or combined with other antineoplastic agents for lung, esophgeal, and cervical cancers was sarially monitored with conventional audiometry and high frequency audiometry. The results are as follows : no damage, 5 ears(23%) ; low frequency(125∼8,000Hz) damage, 1 ear(5%) ; high frequency(10,000∼20,000Hz) damage, 6 ears (27%) ; and both low and high damage, 10 ears(45%). In conclusion, high frequency audiometry is more useful than conventional audiometty in detection of cisplatin ototoxicity.

토양 과습 조건하에서 수수의 생육특성

윤민헌,권수정,주영환,최주영,우선희 忠北大學校 農業科學硏究所 2021 農業科學硏究 Vol.37 No.2

본문참고

Ringed RTFE 이식편을 이용한 슬관절하 하지동맥 우횔술에서 원위문합부 자가정맥 Cuff 혹은 Patch 설치 효과

윤우성,김영욱,허승 대한혈관외과학회 2003 Vascular Specialist International Vol.19 No.1

소아 청소년 자살기사에 관한 내용분석

윤우상,이영식,이길홍 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.1

This study was carried out to search for basic characteristics of child and adolescent suicides. The author analysed child and adolescent suicide cases reported on daily newspaper. Dong-A Ilbo, from 1981 to 1990. The total number of cases were 144, including 94 boys and 50 girls. The cases were devided into three age groups : early(1-14), middle(l5-17), and late(l8-24) age group. The results of the study were as follows : 1) The suicide cases were more frequent in male. The ratio of male to female was 2 : 1. The peak age was 18(13.2%). Increasing the age, suicidal behavior was more frequent in male than in female. 2) Suicides in child and adolescent were reported most frequently in 1981. But until 1989. sucide cases has not been increased. 3) In occupation and education status, the student was the most frequent group(63.2%). Among them, high school adolescents(22.9%) were most common. 4) Suicides were found to occur most commonly during spring, followed by summer, autumn and winter. But difference was not found on the day of week. Suicides were committed more frequently between 12 MD - 6 P.M. This trend was more common in child. 5) The most frequent place of suicide was the home(48.6%). This trend was more common in female and early age group. 6) The most common methods of suicide were by hanging(25.7%), followed by drug(25.0 %) and jumping(l9.4%). In male suicide was committed by more lethal methods, such as hanging, jumping, weapon firing, but in female the most common method was drug. In early age group, more frequent means of suicide was drug(50%) and hanging(33.3%) but in late age group, the means of suicide were variable. 7) The most frequent motivation of suicide were emotional problem and school problem. Demonstration was more frequent motivation in female. The friend was the most common partner in joint suicide.