Bromocriptine 과 Cyproheptadine 이 Corticotropin-releasing Factor(CRF) 로 자극된 ACTH 분비에 미치는 효과

김진우,최영길,김영설,김광원,김선우,양인명,윤현구,우정택 대한내과학회 1986 대한내과학회지 Vol.31 No.5

Our study was desinged to observe the ACTH response to ovine CRF in healthy men and to observe the effect of bromocriptine or cyproheptine on ACTH and cortisol response induced by ovine CRF in healthy men. We have hypothesis that if hromocriptine or cyproheptatine inhibits the secretion of ACTH induced by ovine CRF, they decrease the secretion of ACTH in the pituitary level. The result were followings: 1) Plasma ACTH reached a peak of 1.5 and 1.6 times the basal level in response to ovine CRF at 10 min, and 30 min, respectively and decreased to the basal level at 90 min. 2) Cyproheptadine or bromocriptine did not inhibit the response of ACTH to ovine CRF. 3) We suggest that cyproheptadine or bromocriptine does not inhibit the ACTH secretion in the normal pituitary gland.

갑상선 세포에서 전사보조활성인자인 CBP와 CIITA의 인터페론-감마 활성 부위에 대한 조절 : CBP and CIITA on GAS(Interferon- γ Activated Site)-Mediated Transcription in Thyroid Cells

채수흥,홍우정,김군순,조영석,김도희,김현진,구본정,송민호,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : In the previous stuides, we identified that the interferon-r activated sequence (GAS) in the 5-flanking region of rat ICAM-1 gene is major element for interferon-r-inducible expression of the gene in rat thyroid cells, FRTL-5. We here, investigated the role of transcriptional coactivators, CBP(CREB binding protein) and CⅡTA (class Ⅱ transactivator) in the modulation of the activity of GAS which could interacts with signal transducers and activators of transcription-1 and 3 (STAT1 and STAT3). Methods : The expression of CBP RNA and protein were quantitated in FRTL-5 after stimulation with interferon-r (IFN-r), thyroid stimulating hormone (TSH), forskolin and methimazole. Direct association of CBP with STAT were analyzed by immunoprecipitation. The transcriptional roles of CBP and CⅡTA in the regulation of GAS were assessed by the cotransfection with their expression vectors with reporters; 5-deletion constructs of rat ICAM-1 promoter or 8xGAS-luc constructs, into FRTL-5 thyroid cells. Results : The level of CBP RNA and protein were not changed by the treatment with TSH, IFN-r, forskolin and methimazole in FRTL-5, FRT and BRL liver cells. The CBP could be directly associated with STAT1. Furthermore, the overexpression of CBP significantly increases the both promoter activities; rat ICAM-1 gene promoter which has GAS element and 8xGAS-luc cassette constructs. However, the cotransfection of CⅡTA decreased the constitutive and CBP-mediated transactivation of rat ICAM-1 promoter and 8xGAS-luc cassette constructs. Conclusion : We identified that the two tanscriptional coactivators; CBP and CⅡTA has differential roles in the regulation of transcriptional activity of GAS drived promoter. CBP increases the GAS activity through the direct binding with STAT1, but CⅡTA inhibited the CBP-mediated transactivation of GAS activity.

일단 교류/직류 변환기에서 단일권선 자기에너지 궤환기법에 관한 연구

김우현,강현구 영남이공대학 2003 論文集 Vol.32 No.-

A novel single-switch, single-stage, AC/DC forward converter is proposed using transformer magnetic energy feedback technique for power factor correction. The operational principle and analysis of the proposed converter is presented It gives the good power factor correction, low line current harmonic distortions by using a magnetic energy feedback technique without adding winding. Furthermore, it is capable of producing an isolated output voltage regulation without ripple. Under the IEC1000-3-2 requirements, a unity power factor is nearly achieved by the prototype. In this paper, it's theoretical analysis is presented. The proposed method is successfully verified by computer simulations and experiments.

Inhibition of Cytokine-Mediated Nitric Oxide Synthase Expression in Rat Insulinoma Cells by Scoparone

Kim#, Eun Kyung,Kwon#, Kang Beom,Lee, Ju Hyung,Park, Byung Hyun,Park, Jin Woo,Lee, Hern Koo,Jhee, Eun Chung,Yang, Jeong Yeh Pharmaceutical Society of Japan 2007 Biological & pharmaceutical bulletin Vol.30 No.2

<P>Cytokines produced by immune cells infiltrating pancreatic islets are important mediators of β-cell destruction in insulin-dependent diabetes mellitus. Scoparone (6,7-dimethoxycoumarin) is known to have a wide range of pharmacological properties <I>in vitro</I>. In this study, the effects of scoparone on cytokine-induced β-cell dysfunction were examined. Presence of scoparone significantly protected interleukin-1β (IL-1β) and interferon-γ (IFN-γ)-mediated cytotoxicity of RINm5F, a rat insulinoma cell line, and preserved glucose-stimulated insulin secretion in rat pancreatic islets. Scoparone also resulted in a significant reduction in IL-1β and IFN-γ-induced nitric oxide (NO) production, a finding that correlated well with reduced levels of the inducible form of NO synthase (iNOS) mRNA and protein. The molecular mechanism by which scoparone inhibited iNOS gene expression appeared to involve the inhibition of NF-κB activation. These results revealed the possible therapeutic value of scoparone for the prevention of diabetes mellitus progression.</P>

한 합금공장에서 집단 발생환 급성 베릴륨 질환

김현주,정우철,지영구,김대성,강성규,노상철,조규탁,김동현 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.1

목적: 같은 공장에서 일했던 노동자들 중 원인미상의 급성 폐장염 2례가 발견된 것을 계기로 한 합금공장에서 집단 발생한 화학적 폐장염의 원인을 규명하기 위하여 역학조사를 실시하였다. 방법: 연구대상은 한 합금공장에서 일했던 생산직 노동자 45명 전원이었다. 작업환경측정 기록을 검토하고 일반 폐기능검사,흉부방사선 촬영,작업종료후 요중 베릴륨 농도측정을 포함한 특수건강진단을 실시하였고,유증상자와 유소견자 11명에 대하여 이차로 고해상도 컴퓨터 단층 촬영 및 폐확산능 검사를 실시하였다. 결과: 베릴륨의 공기 중 농도를 평가한 결과 베릴륨이 O.42∼112.3 μg/m³이었고, 요중 베릴륨 농도는 용해 (O.53±0.79 μg/g. creatinine),금형(1.41±0.50 μg/g. creatinine),사상(1.16±0.53 μg/g. creatinine)순으로 높았으나 통계적으로 유의한 차이는 없었다. 일차 특수건강진단결과 용해공정은 비용해공정보다 현재 기침 (p=0.054), 호흡곤란(p= 0.030),입사이후 호흡기증상으로 인한 투약경험률(p=0.018)은 모두 용해작업자가 비용해공정보다 높았으며 일반 폐기능 검사결과는 두 군 간에 차이가 없었다. 최종적으로 화학성 폐장염으로 분류된 사람은 모두 11명으로 그 발생률은 용해공정에서 32.0%,비용해 공정에서 5.0%이었다(p=O.012). 2002년 12윌 이전 입사자에서 발생률은 33.3%이었고 그 이후 입사자에서는 환자가 발생하지 않았다(p<O.001). 결론: 급성 폐장염이 대부분이 용해공정에서 발생했고 모두 환기시설이 설치되기 전부터 작업했던 점으로 보아 이는 급성 베릴륨질환의 가능성이 매우 높다 이는 적절한 작업환경관리로 충분히 예방할 수 있는 질병이므로 앞으로 이러한 문제를 예방하기 위한 정책적 대안을 제시하였다. Objectives: We conducted an epidemiological survey to inquire into an outbreak of acute pneumonitis after two reported cases of interstitial lung disease. Methods: The study subjects were 45 workers from a compound metal alloy factory. We reviewed the factory's industrial hygiene data along with the results of a special health examination, including pulmonary function tests, simple chest X-rays, and high resolution computed tomography. Results: The air concentrations of beryllium ranged from 0.42 μg/m³ to 112.3 μg/m³, and the mean concentration of urinary beryllium were 1.53±0.79μg/g of creatinine in the molding workers, 1.41±0.50μg/g, of creatinine in the casting workers, and 1.16±0.53μg/g of creatinine in the sorting workers. The rates for cough (p=0.054), dyspnea (P=0.030), and the use of medical services (p=0.018) were higher in the molding workers than in the non-molding workers. The incidence rate of acute interstitial lung disease was higher for the molding process (32.0%) than for the non-molding process (5.0%) (P=0.012). The time of employment for all patients was prior to December 1^(st), 2002. Conclusions: Since most of the patients were molding workers, and all of the patients had worked without a ventilation system, this outbreak of acute interstitial lung disease was regarded as acute beryllium disease. Although the direct cause of the epidemic was the beryllium fumes, the fundamental cause was improper control of the work environment. Therefore, the means for preventing avoidable epidemics of occupational diseases are discussed.

무용 전공별 체격·신체구성·체형에 관한 연구

김상은,구광수,김우진 창원대학교 체육과학연구소 2003 경남 체육연구 Vol.8 No.1

본 연구는 무용 전공별의 체격과 신체구성 그리고 체형을 연구하기 위하여 부산과 경남지역에 거주하며 B대학교와 C대학교에서 무용을 전공하는 여학생들 중 한국무용 35명, 현대무용 32명, 발레 26명 총 93명을 대상으로 조사하였다. 조사된 자료는 일원변량분석을 통하여 다음과 같은 결론을 얻었다. 1. 무용 전공별 신장은 현대무용과 발레에서 차이가 있었으나, 흉위와 하지장은 차이가 없었다. 2. 무용 전공별 체중과 좌고는 현대무용과 발레 그리고 한국무용과 발레에서 차이가 있었으나, 체지방률, 상완배부, 장골능상부, 하퇴배부, 상완골단폭은 차이가 없었다. 3. 무용 전공별 체지방량, 제지방량, 체수분량은 현대무용과 발레에서 차이가 있었고, 견갑골 하부는 한국무용과 현대무용에서 차이가 있었다. 4. 무용 전공별 대퇴골단폭과 굴위상완위는 한국무용과 발레 그리고 현대무용과 발레에서 차이가 있었고, 하퇴위는 현대무용과 발레에서 차이가 있었다. 5. 무용 전공별 내배엽요소는 차이가 있었고, 중배엽, 외배엽요소는 차이가 없었으며, 내배엽 요소의 사후검증결과 전공간의 차이는 없었다. 6. 무용 전공별 체형은 한국무용과 발레는 외배엽성의 중배엽인 F이고, 현대무용은 중배엽성의 외배엽형인 H이며, 전공자들의 전체적인 체형은 아주 전형적인 중간체형인 M인 것으로 나타났다.

가토에서의 삽입물 피막피판에 관한 실험적 연구

구상환,김우경 고려대학교 의과대학 1995 고려대 의대 잡지 Vol.32 No.1

Silicones are a group of inert and inorganic compounds that were introduced over three decades ago and can be prepared in many different forms, which vary from hard rubber to the liquid crystals. Use of the silicone is widely accepted in almost every medical and dental specialty, perhaps most commonly in reconstructive surgery. A silicone implant elicits a capsular response in surrounding soft tissue. This phenomenon is well documented since significant capsular contracture occurs commonly as a complication of breast implants. The capsules are built up of an extremely tough, tendon-like collagenous material, which is arranged in a layer and surrounds the prosthesis completely. Three to four weeks after insertion of the implant, the capsule is already formed and it is made of capillaries, larger blood vessels, active macrophages, fibroblasts and a small amount of collagen. Later the capsule becomes less vascularized, more and more collagen are laid down and macrophages become inactive. The aim of this study was to examine whether this side effect could be used as a new source of flaps since the capsule has a structure and texture similar to fascia. With this objective, capsule formation was induced in rabbits by implanting spherical tissue expanders under the skin vascularized by the inferior epigastric vessels. When expansion was completed and the capsule was formed, the expander was removed and the animals were categorized into three groups. In the first group, the roof of the capsule was simply everted as hinge flap. In the second group, a capsule island flap was raised and orthotopically placed. In the third group, a capsule flap was raised and transferred to the ipsilateral injured Achilles tendon area for evaluation of ability to provide gliding surface. Four months later, a tendon-capsule-skin unit was excised and microscopically studied for late changes of capsule flap. The inner side of the various flaps for the first and second experimental groups and the outer side of the island flaps for the third group were covered with autologous full thickness skin graft to find out whether a peri-implant capsule was an adequate recipient site and this might be a new source of flaps for use in reconstructive surgery and this might be a new source of flaps for use in reconstructive surgery. In the three experimental groups, there was complete 'take' of the skin grafts in 80% of the animals. In conclusion, island or free flaps of capsular tissue may be raised and transferred safely. And the capsular flap, which provides a good gliding surface without adhesion even in the late stage, can be very useful if it is used as a fascia-like tissue to cover tendons.

Pb(SCN)₂-KClO₃-Sb₂S₃-TNT 혼합물의 연소에 관한 연구

김우식,구자현,염경호 연세대학교 산업기술연구소 1985 논문집 Vol.17 No.2

The study on the combustion of the Pb(SCN)₂-KCIO₃-Sb₂S₃TNT mixtures are performed with the changes of several operational factors, and its results are as follows; In the drying method, as the increase of the inserting of binders, the impact sensitivity is decreased when the weight of the drop ball is higher, and F, S and ?? of the non-binding mixtures are satisfactory. In the wet method, the squib of mixtures are founded and F, S and ?? are less satisfactory than drying methods, but the impact sensitivity are more satisfactory than drying method. In the measurement of the explosion-delay time of mixtures, the mixtures in drying method are more uniform composition than that in wet method.

지주막하출혈에 의해 발생한 Takotsubo 심근병증의 1예

김선영,김우식,박종혁,오명기,권선옥,나종천,조욱현,최석구 인제대학교 2007 仁濟醫學 Vol.28 No.-

A 64-years-old man was admitted to emergency department with symptom of chest pain. The characteristic of symptom was squeezing pain at substernal area. The patient had a history of hypertension and diabetes mellitus. His electrocardiogram(ECG) showed ST elevation in lead Ⅰ, Ⅱ, V3-6 and his laboratory results revealed mild increase in cardiac enzymes. And his echocardiogram showed apical hypokinesis. Based on the suspicious symptoms and results for acute anterior myocardial infarction, we decided to take a coronary angiogram for further evaluation. However, coronary angiogram revealed normal epicardial coronary arteries. He was admitted at Intensive Care Unit for monitoring and treatment of heart failure. After 3 days of hospitalization, his mental status was changed. His brain computed tomography revealed subarachnoid hemorrhage. After 5 days, hypokinesis of apex was improved to nearly normal on echocardiogram. This is a case of transient left ventricular apical ballooning or takotsubo cardiomyopathy associated with subarachnoid hemorrhage.

優良室素固定菌의 探索分離 및 生理的 特性

朴愚喆,李光熙,金進鎬,李麟九,曺晋基 慶北大學校出版部 1987 慶北大農學誌 Vol.5 No.-

This experiment was conducted to search for Rhizobia with good nitrogen fixation abilities and to investigate their physiological characteristics isolated from 12 soybean cultivars and the affinities of root nodule bacteria with soybeans. The results obtained were as follows ; Based on colors, Rhizobia grown on YMA medium were divided into 3 groups, i. e., white, translucent and transparent, amounting to 60, 30 and 10%, respectively. In litmus milk reation, the strains which produced alkali, acid serum, alkaline serum and acid reached to 51, 29, 9 and 11%, respectively. Strains, S022, and S096 were slow-growers and produced alkaili, while strains, S080, S090, and S118 were fastgrowers and produced acid. The growth of root nodule bacteria on YMA medium was favorable between the initial pHs of 6.0~7.0. Glutamine, asparagine and allantoin as nitrogen sources enhanced the growth of root nodule bacteria. All the strains tested formed nodules on the soybean roots, and the strains with good symbiotic nitrogen fixation abilities that had white color, small colony, nitrate reduction abilities and no nitrite reduction abilities showed comparatively high nitrogen fixing activities. Some strains varied in nitrogen fixing activities according to soybean cultivars, and a few strains formed ineffective nodules which showed no nitrogen fixing activity.