Aptamer‑linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)

Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

20대 성인 남녀 발의 형태분류와 유형별 특성 분석

辛璇遇,咸玉相 服飾文化學會 1999 服飾文化硏究 Vol.7 No.1

For a ideal construction of shoes, the exact analysis for shape and size of foot is necessary. This study was performed to classify and analyze the foot types of young males and females. For this, methods of cluster analysis, correspondence ananlysis and value of proportion were used. The subjects were 414 college students and 36 items, weight(1 item), height(8 items), length(6 items), breadth(6 items), girth(7 items), depth(3 items), slope(5 items) on foot were measured. The fundamental results of the study were as follows ; Difference in foot shape between men's and women's feet was not found below ankle girth. Based on cluster analysis the shapes of foot were classified into five groups, small but relatively slim group, small but relatively barrel group, middle group, large but relatively slim group and large but relatively barrel group. The types of foot had the distinguished dominant characteristics according to sex, weight and height. The results of this study show that there must be the suitable design of shoes for each foot type.

신체 좌우차에 대한 인간공학적 연구

신선우 東國專門大學 1993 金龜論叢 Vol.1 No.1

The purpose of this study is to develop the clothes pattern for women on the basic of their physical differences between right and left items. 101 college students participated in measurement. Including the foot size, thirty two items of human body were collected. Descriptions and statistical analysis of the anthropometric data are summarized. The statistical methods used in this paper were discriptive statistics, F-test, correlation analysis, stepwise regression, factor analysis, etc.

교직원 중 비흡연자와 흡연자의 건강상태비교

신선미,이희우 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.2

Purpose:This study estimated the prevalence of smoking among male and female school employees. This study also correlated key indicators of health with the number of cigarettes consumed per day by the male smokers. The indicators of health included health behavior, blood pressure (mmHg), BMI (kg/m2), diabetes, total cholesterol, GOT, GPT and GTP. Methods:Subjects included 2,640 male and 2,747 female employees working at elementary, middle, and high schools who received a physical check-up at the Seoul School Health Center in 2002. Data was obtained from the physical examinations, questionnaires, and laboratory results. Results:39.7% of males and 0.4% of females were current smokers. 60% of the male smokers consumed between 10 and 19 cigarettes per day, and39.6% of male smokers had been smoking between 10 and 19 years. Smokers who consumed more than one pack per day had a relatively higher BMI than the non-smokers (24.7 24.1, respectively). However, smokers had lower blood pressure than non-smokers. The mean systolic and diastolic blood pressure of current-smokers were 128.2 and 82.8, respectively, while the values among non-smokers were 129 and 84.5, respectively. The proportion of current smokers with severe obesity (over BMI 30) was 5.0%. This was considerably higher than the 1.3% of severely obese non-smokers. Current-smokers also had mean and abnormal rate of fasting blood glucose, total cholesterol, GOT, GPT, and GTP that were higherthan those of non-smokers. The frequency of red meat consumption, alcohol use, perceived incidents of stress were alsomore frequent in current-smokers than in non-smokers. Exercise frequency was also lower among smokers. In multiple regression after adjusting all possible confounding factors including age, BMI, diet, and drink, the parameter value of fasting blood glucose, cholesterol, GOT, GPT, and GTP increased relative to the level of cigarette consumption. Conclusion: Among school employees, health behavior, and general health status including BMI, total cholesterol, GOT, GPT, GTP, and fasting blood glucose were worse in smokers than in non-smoker.

정신분열병에 대한 Risperidone의 효과 및 안전성에 관하여

신석철,왕성근,지익성,이선우,이규광,이봉희,이진영,황선희,신용재,배경도,김정란 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

In order to evaluate the efficacy and safety of risperidone, 10 patients with chronic schizophrenia were examined for 8 weeks in a silgle-open study. After the wash-out period of 1 week, risperidone was administered. Efficacy was evaluated by means of Positive and Negative syndrome Scale for Schizophernia(PANSS) and Clinical Global Impression(CGI). Safety assessment included the Extrapyramidal Symptom Rating Scale(ESRS), vital signs, body weight, ECG, and laboratory tests. The results were as follows 1) On the PANSS total score, there was significant improvement of total score after 1 week (P<.05) of administratiom. 2) On the PANSS positive and negative subscal, there were significant improvement of posotive and negative scores after 2 weeks (P<.05) of administration. 3) On the PANSS general psychopatholgy subscals, there were significant improvement of general psychopathologyscores after 1 week (P<.05) of administratiom. 4) On the CGI, there was significant decreasement of clinical impression of severity of schizophrenia score after 2 week (P<.05) of administration. 5) A statistically significant increase in body weight (P<.05) was observed after 8 weeks of administration. 6) EPS reached the peak at the end of the 1st week of administration of risperidone (11.8± 24.25) but they were easily relieved by addition of benztropine and clonazepam. There was not significant change in laboratory tests, vital sign, ECG after 8 weeks of administration. These results suggest that Risperidone is an effective antipsychotics and clinically safe except for increased body weight in chronic schizophrenia.

뇌경색후에 발생한 이차성 조증

신석철,이충숙,지익성,이선우 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

Mania is a rare complication of stoke. The clinical features and response to treatment are usually the same as mania without brain injury. Post-stroke mania strongly associated with both a right hemisphere lesion in a limbic-connected area and a second predisposing factor, such as genetic loading for a affective disorder, pre-existing subcortical atrophy or seizure disorder. It may be mediated through frontal lobe dysfunction. We report a case of secondary mania in 65-year old man who showed multiple lacunar infarction left thalamus and right periventricular area.

서울시 교직원의 고혈압 실태파악과 그 요인에 관한 연구

신선미,김종희,한규종,이희우 韓國學校保健學會 2004 韓國學校保健學會誌 Vol.17 No.1

Purpose : To describe epidemiological characteristics and related factors in school personnel with hypertension. Method : 5,384 school personnel (2,638 males and 2,746 females) received physical examination at the Seoul School Health Center in 2002. blood pressure(BP) was classified by JNC 7th report. Normal BP is systolic BP <120 and diastolic BP<80, prehypertension BP is 120-139 or 80-89mmHg, stage 1 hypertension BP is 140-159 or 90-99, and stage 2 hypertension >=160 or >=l00 mmHg. each group was evaluated by gender. We used multiple regression and logistic regression. Results : Normotension was 20.13% in d e s and 48.65% in female, prehypertension 43.4% in males and 3.06% in females, stage 1 hypertension 26.38% in males and 10.99% in females, stage2 hypertension 9.59% in males and 3.2% in females. the older age group had higher distribution of stage 1 hypertension and stage 2 hypertension. The means and abnormal rates of BMI, blood sugar, total cholesterol, GOT, GPT, and GTP was higher when their blood pressure was higher. However, an alcohol habit among lifestyle factors had an inverse effect. Higher Bp was correlated to a &her BMI, FBS, and cholesterol-like dose response. In stage 1 hypertension, the related factors of hypertension for males were BMI, amount of cigarettes smoked, exercise, blood sugar, total cholesterol, and GTP. In stage 1 hypertension, age, BMI, exercise, blood sugar, total cholesterol were related factors in female. In stage 2 hypertension, age and smoking were related factors in male, and age, BMI, and cholesterol in female. Conclusions : It is possible to intervene in all related factors of hypertension except age through life-style modification and appropriate medical management. Active health promotion is needed in School personnel.

군집분석을 통한 발의 유형별 특성

신선우 東國專門大學 2002 金龜論叢 Vol.9 No.1

성인 남녀의 발의 유형화하여 분류하고 유형화된 결과에 대한 특성을 파악하기 위하여 20대 남녀 414명을 대상으로 하여 체중, 하지의 8개 높이 항목, 6개 길이 항목, 6개 너비 항목, 7개 둘레 항목, 3개 깊이 항목, 각도 5개 항목의 총 36 항목을 인체계측하여 그 계측치에 대한 군집분석을 실시하였다. 군집분석에서 유사성의 척도로는 유클리드 거리(Euclidian distance) 측정방법을 사용하였고 분류된 유형의 차이를 밝히기 위해 36항목에 대한 F-test를 설시하고 다중비교검정으로 던컨 테스트를 실시한 결과 발형태는 5개의 유형으로 분류되었는데 유형 1은 발이 전체적으로 작으며 발둘레에 비해 발이 긴 유형, 유형 2는 발이 전체적으로 작으며 발둘레에 비해 길이가 짧은 유형, 유형 3은 평균적 크기의 유형, 유형 4는 발이 전체적으로 크며 발의 길이에 비해 굵은 유형, 유형 5는 발이 전체적으로 크며 발둘레에 비해 길이가 긴 형태의 유형이다. 유형별 특성 분석 결과 유형 1은 여자집단에 주로 속하며 체중은 50-55kg, 신장은 156-165cm에서 출현율이 높았다. 유형 2는 주로 여자집단으로, 체중은 45-50kg, 신장은 156-165cm에서 높은 출현율을 나타내었고, 유형 3은 여자집단, 체중은 60-65kg, 신장은 156-165cm, 유형 4는 남자집단, 체중은 80-85kg, 신장은 176-185cm, 유형 5는 남자집단, 체중은 65-75kg, 신장은 166-175cm에서 출현율이 높게 나타났다.

인듀서 내부의 캐비테이션 유동과 흡입성능에 관한 연구

申澈洙,金鍾喆,姜宇政,李先基 충주대 산업과학기술연구소 1995 産業科學論文集 Vol.3 No.-

In order to understand the influence of the inducer inlet angle on cavitation and suction performance, experiments were carried out for two kinds inducer, which have the same outlet angle distribution, length and number of inducer blades. The results showed that the change of inducer inlet angles influenced not only internal flow conditions but also cavity flow and suction performance. The improvement of suction performance by using inducer were also discussed.