양식장 배출물 발효물의 어류 사료 첨가에 따른 넙치, Paralichthys olivaceus의 혈액학적 변동에 미치는 영향

강주찬,지정훈,송승엽,문상욱,강지웅,이영돈,김세재 한국어병학회 2004 한국어병학회지 Vol.17 No.1

본 연구는 우리나라 주요 양식어류인 넙치, Paralichthys olivaceus를 대상으로 육상수조식양식장 배출물 발효물의 어류 사료 첨가에 따른 혈액학적 변동에 미치는 영향을 검토하였다. 젖산균 및 효모를 사용하여 발효한 양식장 배출물의 이화학적 분석을 실시하였고, 발효물을 0.1, 0.5, 1.0 및 5.0% 농도로 8주간 투여하였다. 투여 4주째 및 8주째에 각 투여 구간별로 혈액학적 항목을 중심으로 그 차이를 확인하였다. 배출물 발효농도별 유의한 차이는 나타나지 않았지만, 분석시기에 따른 적혈구수, 혈색소 농도 및 혈색소 지수가 증가하는 경향을 나타내었으나, 주요 혈청 무기 및 유기성분은 배출 발효물 농도별로 유의한 차이는 나타나지 않았다. 또한 혈청 효소학적 조사에서는 조사시기별로 변동은 나타났지만, 동일 조시시기에서 각 구간별 유의한 차이는 나타나지 않았다. 따라서 이러한 육상 약식장 유래의 배출물 발효산물에 대한 다양한 방면의 연구가 진행되어야겠다. Effects of oral administration with fermented product from sewage in land-based seawater fish farm on haematological disturbance in the olive flounder, Paralichthys olivaceus was investigated. After 4 weeks of conditioning with a basal diet, fish were divided into 4 groups and provided experimental diet (0.1,0.5, 1.0 and 2.0%) supplement of fermented sewage for 80 days. Proximal analysis was performed for the product of sewage which was fermented by lactic acid and yeast. RBC count, hemoglobin concentration and hematocrit value were increased according to the treated periods, however, no statistical difference was observed between control and treatment groups. There were no significant difference in serum organic, inorganic compounds and enzyme activities between control and treatment groups. This study hypothesized that the supplement of fermented product from sewage in land-based seawater fish farm might be an additive sup plement for source of fish diet in view of haematological examination. Recycling of the sewage may be an economic artificial sources of diet for fish aquaculture practices.

MANET 라우팅 프로토콜 QoS 지원서비스의 성능평가에 관한 연구

鄭燦赫,金鉉郁,李光培,姜景仁,朴京培,劉忠烈,吳世德,裵振勝,趙榮泰 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In this paper, we evaluated two main QoS supporting routing protocol on wireless ad hoc network. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS support service between source and destination becoming a very important issue. Of all routing protocols, DSR and AODV are very important routing protocol in MANET. So we simulated DSR and AODV QoS Routing Protocol Through simulation evaluation tool NS(Network Simulation) based on various environments.

이동 애드 혹 네트워크에서 QoS를 지원하는 AODV 라우팅 프로토콜

吳世德,趙榮泰,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In this paper, we propose a bidirectional mobile Ad Hoc routing protocol based on AODV(ad-hoc on-demand distance victor routing) with QoS(quality of service) support. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS guarantee between end point is a very important issue. The existing AODV routing protocol is implemented to use only BE (Best Effort) service. However, actual wireless mobile environment requires to reduce a waste of communication resources and meet with the real-time change of data paths by setting up routes with QoS support, considering available communication resources at each node in advance. Therefore, in this thesis, we propose an adaptive QoS support method using hello message in order to rapidly meet with the change of available communication resources and to smoothly perform route maintenance. For performance evaluation, we analyzed the average date reception rate. At that time, we used evaluation parameters such as node's mobility -stop time, hello message period, and packet priority, considering the node's mobility and the number of best effort/QoS data flows. As results, we found that with our proposed method the average data reception rate was increased 16.3% for 0 mobility-stop second case and 4.7% for 300 mobility-stop second case.

지난 2년간의 학회지 "신경정신의학" 심사 평가보고

김찬형,김세주,김도관,김성곤,박원명,송동호,박용천,이영문,오강섭,정영철,이상열,김주한,이동영,이헌정,함병주,김율리,김문두,김정진,이강욱,천근아,김재진,홍진표,홍승철 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

재발 또는 불응성 비호즈킨 림프종 환자에서 CDME 구제항암화학요법 후 고용량 항암화학요법 및 자가말초혈액 조혈모세포이식의 효과

김세형,한강원,배상병,김찬규,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background and objectives : The long-term survival in patients with non-Hodgkin's lymphoma (NHL) after conventional dose chemotherapy is about 35% and the rest of the patients tend to have relapse. So, in relapsed or refractory NHL, we compared the outcome of patients undergoing high-dose chemotherapy with autologous peripheral blood stem cell transplantation(APBSCT) with only salvage chemotherapy of cisplatin, dexamethasone, mitoxantrone, and etoposide(CDME). Materials and methods : From June 1993 to December 1999, 25 patients with relapsed or resistant NHL were treated with CDME regimen as salvage chemotherapy. Twelve patients were received four cycles of CDME chemotherapy, and 13 patients were received high-dose chemotherapy with APBSCT following two cycles of CDME chemotherapy. Results : The median follow-up duration was 12.8 months(range:4-68). The overall response rate was 41.7% (complete response rate 25%, partial response rate 16.7%) in 12 patients with CDME only. Thirteen patients who were treated with high-dose chemotherapy with APBSCT achieved 61.5% complete response rate and 15.4% partial response rate, with an overall response rate of 76.9%. The estimated 3-year progression-free survival rate was significantly higher among patients who received high-dose therapy than patients who received CDME only(41.5% vs 20.0%, p<0.05). And, 3-year overall survival rate was significantly higher among patients who received high-dose therapy(51.3% vs 25.0%, p <0.05). Conclusions : In relapsed or refractory NHL, CDME chemotherapy is an effective salvage chemotherapy and allow peripheral blood stem cell collection. Also, high-dose chemotherapy with APBSCT following CDME is superior to CDME salvage chemotherapy only.

요추부 유합수술 후 가동인접분절의 후기변화

김홍태,강도원,유찬훈,정재호,장세앙 대한척추외과학회 1996 대한척추외과학회지 Vol.3 No.1

The spinal fusion in a lumbar region may influence biomechanically in the remained mobile segment to take over the lost motions Therefore the stress concentration on the adjacent segments may accelerate the degenerative changes, and then various late changes could occur. The aim of this study was to evaluate the late changes occuring in the adjacent segments to lumbar fusions. A retrospective review of radiographs and medical records was undertaken for 67 consecutive patients who had undergone various fusions in the lumbar region for various pathologic conditions. Included in this study were the patients who had performed active daily livings without any significant pain or disability after fusion and followed for a minimum of five years(up to 18 years with a mean of 8.3 years). The results of this study were as follows : The instability, disc narrowing, spinal stenosis, vertebral slipping, or isthmic defect were found in the adjacent segments to fusions in the patients who were followed longer, in L4-5 segment and above segment of fusion, and in the adjacent segments where a degenerative changes existed before surgery and where the angular motion increased considerably in a few years after fusion. The symptoms of these patients were responded well with conservative theraphy and no patient needed any surgical theraphy during these follow-up periods.

시클러 캡슐(세파클러 250㎎)에 대한 경보세파클러 캡슐의 생물학적동등성

조혜영,강현아,김세미,박찬호,오인준,임동구,문재동,이용복 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.1

The purpose of the present study was to evaluate the bioequivalence of two cefaclor capsules, Ceclor (Lilly Korea Co., Ltd.) and Kyongbocefaclor (Kyongbo Pharm. Co., Ltd.), according to the guidelines of the Korea Food and Drug Administration (KFDA). The release of cefaclor from the two cefaclor formulations in vitro was tested using KP VIII Apparatus II method with various dissolution media (pH 1.2. 4.0. 6.8 buffer solution and water). Twenty four healthy male subjects. 22.96±1.52 years in age and 67.03±7.90 kg in body weight, were divided into two groups and a randomized 2x2 cross-over study was employed. After one capsule containing 250 mg of cefaclor was orally administered, blood was taken at pre-determined time intervals and the concentrations of cefaclor in serum were determined using HPLC method with UV detector. The dissolution profiles of two formulations were similar at all dissolution media. In addition. the pharmacokinetic parameters such as AUC_(t), C_(max) and T _(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t). C_(max) and untransformed Tmaa. The results showed that the differences between two formulations based on the reference drug, Ceclor. were -1.90%, 2.68% and -7.60% for AUCt, C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log 0.8 to log 1.25 (e.g., log 0.91-log 1.06 and log 0.92-log 1.18 for AU', and C_(max), respectively). Thus. the criteria of the KFDA bioequivalence guideline were satisfied, indicating Kyongbocefaclor capsule was bioequivalent to Ceclor capsule.

비유피-4 정(염산프로피베린 20㎎)에 대한 건일염산프로피베린 정의 생물학적동등성

조혜영,박은자,강현아,백승희,김세미,박찬호,오인준,문재동,이용복 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.5

The purpose of the present study was to evaluate the bioequivalence of two propiverine hydrochloride tablets. BUP-4 (Jeil Pharm. Co., Ltd.) and Kuhnil Propiverine Hydrochloride (Kuhnil Pharm. Co., Ltd.), according to the guidelines of the Korea Food and Drug Administration (KFDA). The propiverine release from the two propiverine hydrochloride formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method with a variety of dissolution media (pH 1.2, 4.0, 6.8 buffer solutions, water and blend of polysorbate 80 into pH 6.8). Twenty six healthy male subjects, 23.73 ± 2.79 years in age and 67.04 ± 7.93 kg in body weight, were divided into two groups and a randomized 2 x 2 cross-over study was employed. After one tablet containing 20 mg as propiverine hydrochloride was orally administered, blood was taken at predetermined time intervals and the concentrations of propiverine in serum were determined using HPLC method with UV detector. The dis-solution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC" C _(max) and T _(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC, C_(max), and untransformed T_(max). The results showed that the differences between two formulations based on the BUP-4 were 0.17%, 7.98% and 4.55% for AUC,, C_(max), and respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically trans-formed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.88)-log(l .12) and log(0.90)-log(l.15) for AUC, and _(max), respectively). Thus, the criteria of the KFDA bioequivalence guideline were satisfied, indicating Kuhnil Propiverine Hydrochloride tablet was bioequivalent to BUP-4 tablet.

Anti-Proliferative Effects of Estrogen Receptor-Modulating Compounds Isolated from Rheum palmatum

Kang, Se-Chan,Lee, Chang-Min,Choung, Eui-Su,Bak, Jong-Phil,Bae, Jong-Jin,Yoo, Hyun-Sook,Kwak, Jong-Hwan,Zee, Ok-Pyo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

The Rheum palmatum L., a traditional medicine in Korea, was screened for their estrogenic activity in a recombinant yeast system with a human estrogen receptor (ER) expression plasmid and a reporter plasmid used in a previous study. The $EC_{50}$ values of the n-hexane, dichloromethane, ethyl acetate, n-butanol, and water fractions of the methanolic extract of R. palmatum in the yeast-based estrogenicity assay system were 0.145, 0.093, 0.125, 1.459, $2.853\;{\mu}g/mL$, respectively, with marked estrogenic activity in the dichloromethane fraction. Using an activity-guided fractionation approach, five known anthraquinones, chrysophanol (1), physcion (2), emodin (3), aloe-emodin (4) and rhein (5), were isolated from the dichloromethane fraction. Compound 3 had the highest estrogenic relative potency (RP, 17bestradiol = 1.00) ($6.3{\times}10^{-2}$), followed by compound 4 ($3.8{\times}10^{-3}$), compound 5 ($2.6{\times}10^{-4}$), and compound 1 ($2.1{\times}10^{-4}$). Also, compound 3 and fraction 3 (which contained compound 3) of the dichloromethane fraction of R. palmatum showed strong cytotoxicity in both ER-positive (MCF-7) and -negative (MDA-MB-231) breast cancer cell lines.

Development of disease models for non-clinical evaluation of arteriosclerosis therapeutic agents

Se Chan Kang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

CAD(Coronary artery disease) is a chronic vascular disease in which fibrous proliferation occurs in the arterial lining caused by inflammation, endothelial cells, leukocytes, and vascular muscle cells, and cholesterol is known as the main factor. There is currently no direct treatment, and statin drugs are being prescribed to lower cholesterol levels. Many researchers are working on developing atherosclerosis treatments, but existing animal models have limitations that the effect cannot be reproduced in human, so it has been needed to create a new atherosclerosis animal model. Therefore, we sought to establish a system for the development of direct atherosclerosis treatments by developing a human-like rat model and discovering biomarkers that could link nonclinical to clinical research. First, we investigated the database associated with the atherosclerosis rat models to select 17 candidate genes. After clustering them, the final four genes, ApoE, Ldlr, Cyp17A1 and PRKAA1, has been selected by using the Grady Best-first Search (GBS) algorithm. In addition, the possibility of a human resemblance atherosclerosis animal model has been confirmed by producing rats according to overexpression and knock-out and KO rat of the selected gene. The development of human resemblance atherosclerosis rat model can contribute to the reliable development of treatment in the future, and it is believed that it will be able to contribute to the establishment of a nonclinical-clinical system related to various diseases.