A Case of Occupational Hypersensitivity Pneumonitis Associated with Trichloroethylene

Jae, Young,Hwang, Eu Dong,Leem, Ah Young,Kang, Beo Deul,Chang, Soo Yun,Kim, Ho Keun,Park, In Kyu,Kim, Song Yee,Kim, Eun Young,Jung, Ji Ye,Kang, Young Ae,Park, Moo Suk,Kim, Young Sam,Kim, Se Kyu,Chang, The Korean Academy of Tuberculosis and Respiratory 2014 Tuberculosis and Respiratory Diseases Vol.76 No.2

Trichloroethylene (TCE) is a toxic chemical commonly used as a degreasing agent, and it is usually found in a colorless or blue liquid form. TCE has a sweet, chloroform-like odor, and this volatile chlorinated organic chemical can cause toxic hepatitis, neurophysiological disorders, skin disorders, and hypersensitivity syndromes. However, the hypersensitivity pneumonitis (HP) attributed to TCE has rarely been reported. We hereby describe a case of HP associated with TCE in a 29-year-old man who was employed as a lead welder at a computer repair center. He was installing the capacitors on computer chip boards and had been wiped down with TCE. He was admitted to our hospital with complaints of dry coughs, night sweats, and weight losses for the past two months. HP due to TCE exposure was being suspected due to his occupational history, and the results of a video-associated thoracoscopic biopsy confirmed the suspicions. Symptoms have resolved after the steroid pulse therapy and his occupational change. TCE should be taken into consideration as a potential trigger of HP. Early recognition and avoidance of the TCE exposure in the future is important for the treatment of TCE induced HP.

Capsaicin으로 유도된 신경성 염증반응에 미치는 상백피의 영향

윤창용,신동환,홍충만,이원규,장동덕,채옥희,강경진,이무삼 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

All-Trans Retinoic Acid에 의한 HL-60 세포 분화유도중 H2B Histone, c-Myc 및 DNA Topoisomerase I 발현에 관한 연구

임규,박정동,최병한,이용진,김계영,이명선,장은미,김삼용,권기량,곽상태,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Effects of all-trans retinoic acid(retinoic acid) on DNA replication, H2B histone and DNA topoisopmerase I(Topo I) gene expression have been investigated in human promyelocytic leukemia cell line HL-60. DNA synthesis decreased at 24 hours after exposure of retinoic acid in the HL-60 cells. H2B histone mRNA rapidly reduced at 48 hours and Topo I and c-myc mRNA at 24 hours in retinoic acid-exposured HL-60 cells, respectively. The levels of c-myc, H2B histone and Topo I gene expression were reduced in proportion to the concentration of retinoic acid. The H2B histone mRNA in retinoic acid-exposured cells was elevated by cycloheximide treatment, while the level of Topo I mRNA was constant. These results suggest that regulations of H2B histone, c-myc and Topo I gene expression are different from one another, and repression of Topo I gene is closely correlated with c-myc gene during retinoic acid-induced differentiation of HL-60 cells.

cis-Diamminedichloroplatinum (Ⅱ)이 Hamster 근위곡요세관 상피세포의 미세구조에 미치는 영향

정호삼,이창수,이규식 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

cis-Diamminedichloroplatinum (II) (cis-DDP), a metallic compound, similar to bifunctional alkylating agent and has been known as an effective anticancer drug. Cis-DDP is known to inhibit cell division and is so effective to suppress the solid tumors that it has been used in the therapy of acute ovarian tumor, acute epithelial tumor in the neck, bladder cancer and thyroid cancer. cis-DDP reacts with guanine bases of DNA in the cell that it inhibits DNA synthesis, cell division and protein synthesis consequently. It exhibits no specificity with regard to type of cells that it acts on the normal cells as well as cancer cells during therapy. So the author undertook the present study to pursue the effect of cis-DDP on the cytoplasmic organelles of epithelial cell in kidney. Hamster was used as an experimental animals. The experimental animals were killed at 12, 24 and 48 hours after administrations of 6 mg/kg of cis-DDP. The specimen obtained from the renal cortex were fixed and stained with uranyl acetate and lead nitrae. And these preprations were observed with the JEM 100B electron microscope. The results obtained were as follows. 1. The cisternae of rough endoplsmic reticulum were sacculated and membrane bound ribosomes were detached at the epithelium of proximal convoluted tubles in the 24 and 48 hours cis-DDP treated hamster group. 2. Mitochondria were swollen and cristae of mitochondria were not seen at the epithelian cells of proximal convoluted tubules in cis-DDP 48 hours treated hamster group. 3. Vesicles and vacuoles increased in number with time at the epithelium of proximal convoluted tubules in cis-DDP treated hamster groups. 4. Microvilli were segmented at the epithelium of proximal convoluted tubules in cis-DDP 48 hours treated hamster group. Consequently it is suggested that cis-DDP induces the damage in the epithelial cells of proximal convoluted renal tubule.

경동맥 내막 절제술중 뇌보호와 신경생리학적 검사의 의의

신용삼,김선호,김종훈,민경태,김동익,유선국,이규창 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.8

Methotrexate가 Mouse 간세포의 미세구조에 미치는 영향

정호삼,장혁순,이규식 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Methotrexate (MTX), folic acid analog, has been widely used in the therapy of psoriasis and also used in the chorionic adenoma and acute lymphatic leukemia. Methotrexate inhibits not only DNA synthesis but also RNA and protein synthesis. It has been reported that during the therapy, its cytotoxicity leads to necrosis of the epithelium of the renal tubules, hair loss and hepatic fibrosis. So the author undertook the present study to pursue the effect of methotrexate on the cytoplasmic organelles of hepatic cells. Albino mice, ICR strains, weighing 20gm were used experimental animals. The experimental animals were killed at 24, 48, 72 and 96 hours after administrations of 15 mg/kg of MTX. The specimens obtained from liver were prefixed in Millonig's solution and post-fixed in the 1% osmic acid. After specimens were dehydrated and embedded in the Epon 812, ultrathin sections were made and double stained with uranyl acetate and lead citrate. And these preparations were observed with the electron microscope. The results were as follows. 1. The majority of mitochondria was hypertrophied and some mitochondria were irregular shaped, its double membrane and crests disappeared in the mouse liver of the 24 hours-MTX treated group. 2. Golgi complexes were atrophied in the 48 hours-MTX treated group and cisternae of the Golgi apparatus were hypertrophied and vesicular formation was observed in the 72 hours-MTX treated group. 3. The cisternae of the rough endoplsmic reticulum wer dilated and sacculated, but the detachment of membrane bound ribosomes was observed. 4. Lysosomes and autophagic vacuoles were increased in number with time. Consequently, it is suggested that MTX would induced the damge of the organelles of the hepatic parenchymal cells in mice.

Bleomycin이 Mouse 간장의 Alkaline Phosphatase 활성에 미치는 영향

이규식,정호삼,이군자,김종우 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Bleomycin hydrochloride isolated from the Streptomyces veticillus has been widely used as an anticancer drug. In exhibits specific antineoplastic activity against squamous epithelial carcinomas of the skin. Bleomycin causes inhibition of DNA and protein synthesis by incorporating bithiazole group of the drug into DNA. The author undertook the present study to investigate the effect of bleomycin on the alkaline phosphatase activity in the mouse liver. The experimental animals were sacrificed at 6, 12, 24, and 48 hours after administration of 150 mg/kg of bleomycin. The activity of lkaline phosphatase was observed by Gomori's method for histochemical study. The results were as follows. The activity of alkaline phosphatase was decreased in the entire hepatic lobule of the 12 hours belomycin treated group and recovered in the 24 hours bleomycin treated group. Consequently, it is suggested that bleomycin has the cytotoxic effect on the hepatocytes.

흰쥐 肝臟의 組織發生에 관한 組織化學的 및 電子顯微鏡的 硏究

鄭鎬三,李圭植,張世榮 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

Numerous workers have intensively studied for physological and histochemical aspects on the histogenesis and differentiation of mammalian livers focusing attention on its hematopoietic function. In early fetal life hepatic diverticulum formed caudal part of the foregut in separated into two parts by the mesodermal septum each being developed into primordia later. The author has undertaken histological and histochemical studies to persue the morphological and functional changes to occur in hepatic lobules during development and differential period. The pregnant albino rats, Wistar strain, were sacrificed every day starting from 15th to 20th day of gestation. The specimens obtained from the fetal liver were fixed with 10% formalin and stained with hematoxylin-eosin to observe the morphological development and differentiation of the liver. The activities of acid and alkaline phosphatase were observed by the Gomori's methods(1931, 1941) and ATPase activity by the Wachstein-Meisel's method(1957). And also another liver specimens fixed with 4% glutaraldehyde in 0.1M cacodylate buffer(pH 7.4) at 4℃ by the Eranko's method(1952) were examined with electron microscope to investigate phosphatase activities in the hepatic parenchymal cells. The results were as follows. 1. In the liver of the 15th day of the fetus, undifferentiated hepatic parenchymal cells were constituted and a few nucleated erythocytes were spread at the intercellular region, and at the 16th and 17th day of the fetus a number of hematocytoblast were distributed and primitive hepatic cord and hepatic sinusoid were formed. 2. In the Liver of the 18th, 19th and 20th day of the fetus, hematopoietic cells were reduced but the differentiation of he hepatic parenchymal cells, hepatic cord and hepatic sinusoid were observed. 3. The activity of the acid phosphatase increased from the 17th to 20th day of the fetus. High activity of the enzyme at the peripheral and central zone and trace activity at the lysosome and rough endoplasmic reticulum were observed. 4. Moderate activity of the alkaline phosphatase was observed in the liver of the 19th and 20th day of the fetus at the nuclear membrane of the parenchymal cell and at the villi of the bile canaliculi. 5. The activity of the adenosine triphosphatase was observed in the liver of the 18th day of the fetus, and positive activity at the plasma membrane and weak activity at the rough endoplasmic reticulum were observed at the 19th and 20th day of the fetus, respectively.

유전체 충전형 플라즈마 반응기를 이용한 Trichloroethylene의 분해반응

이해완,류삼곤,박명규,박현배,황경창 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.3

유전체 충전형 플라즈마 반응기를 이용하여 공기중 trichloroethylene (TCE)의 분해반응에 대한 연구를 수행하였다. 방전전력, 체류시간 및 반응물 유입농도 등과 같은 여러 가지 운전변수에 따른 TCE의분해효율을 조사하고, 반응 부산물의 분석을 통하여 TCE 분해 반응 메커니즘을 제시하였다. 실험결과 방전전력 및 체류시간이 증가함에 따라 TCE 분해효율은 증가하였으나 유입농도의 변화에 대한 영향은 거의 없었다. BaTiO_3를 충전한 반응기가 알루미나를 충전한 반응기보다 분해효율이 높았으나 TCE의 완전 산화분해의척도가 되는 CO_x(CO+CO_2)의 수율 및 CO_2의 선택도는 BaTiO_3를 충전한 반응기보다 알루미나를 충전한 반응기가 월등하게 높았다. 반응생성물 분석결과 주반응 생성물은 CO_x,CHCl_2COCl, C_2H_2Cl_2O_2및 COCl_2로 공기중 TCE의 분해 메커니즘은 주로 ClO와 OH 라디칼에 의한 반응으로 판단된다. The decompositon of trichloroethylene (TCE) in air using a ferroelectric packed-bed reactor was studied. The effects of discharge power, residence time, inlet concentration and other operating conditions on the decomposition efficiency were investigated and the analysis of reaction products was conducted to suggest the mechanism of TCE decomposition. Experimental results showed that the decomposition efficiency of TCE increased with increasing discharge power and residence time but was unaffected by inlet concentration. The decomposition efficiency for BaTiO_3 packed reactor was higher than that for alumina packed reactor, but the yield of CO_x(CO+CO_2) and selectivity of CO_2 as the measure of the complete oxidation of TCE were higher in alumina packed reactor. The main products of TCE decomposition were CO_x,CHCl_2COCl, C_2H_2Cl_2O_2 and COCl_2. On the basis of the results, it is inferred that the decomposition of TCE in air proceeds by OH and CIO radical reaction mechanism.

상부위장관 출혈의 임상적 고찰

장금석,이승일,박삼규,조건국,장정수 조선의대 1984 The Medical Journal of Chosun University Vol.9 No.1