蛙 脾臟의 組織學的 硏究

鄭鎬三,林洛龍 최신의학사 1975 最新醫學 Vol.18 No.3

Although frog spleen is generally structurally similar to that of mammals, the following differences were observed in our study. . 1. There is only one trabecula in the middle zone of tissue, and the capsule of the frog spleen does not contain smooth muscle fibers. . 2. The white pulp does not have uniform complete nodules, the lymphocytes in the white pulp are not packed close together, the white pulp contains large numbers of erythrocytes, and there are no central arteries or germinal centers in the spleen of frogs. 3. The arteries of frog spleen, unlike those in mammalian spleen, do not branch into central arteries in the white pulp, or into sheathed arteries in the red pulp. 4. Reticular tissue appears near the venous sinuses, but is not widely distributed throughout other splenic tissue. 5. Veins of frog's spleen are distributed beneath the capsule and periphery of trabecula.

흰쥐 폐 발생시 Laminin의 발현에 대한 연구

정호삼,박철홍,백두진,백태경,김원규,윤지희,서윤경,Chung, Ho-Sam,Park, Chul-Hong,Paik, Doo-Jin,Baik, Tae-Kyung,Kim, Won-Kyu,Youn, Jee-Hee,Suh, Yun-Kyung 한국현미경학회 2001 Applied microscopy Vol.31 No.1

Laminin은 세포외기질의 주요 구성 당단백질로 알려져 있고 특히 기저막에 다량 분포되어 있어 제 4형 아교질, heparan sulfate proteoglycan 및 entactin과 결속되어 있다. Laminin은 세포외기질에 함유되어 조직의 발생, 분화 및 성숙 등에 직접 및 간접적으로 관련되어 있으며, 특히 세포의 분열, 이동에 관여하고 조직내 기저막의 물질 투과성에 영향을 미쳐 상피의 분화 및 재형성과 관계있음이 많은 학자들에 의하여 보고되어 있다. 최근에는 laminin이 포유동물에서 태자기와 신생아기에서 폐의 발생과 분화에 주요한 역할을 함도 일부 보고되어 있다. 이에 저자는 폐의 발생과정에서 조직 혹은 세포내에서 laminin의 발현과 분포 변화를 면역조직화 학염색법과 면역도금법을 이용해서 추적하고자 하였다. 실험동물로는 발생 제 14일, 제 16일, 제 18일 및 제 20 일의 태자와 생후 1일, 3일 5일 및 7일의 신생 흰쥐를 사용하였으며, 각 실험동물의 폐조직을 절취하고 면역조직화학염색과 면역도금법으로 laminin의 면역활성의 변동을 조사하여 다음과 같은 결과를 얻었다. 1. 발생 제 14일, 제 16일, 제 18일 및 제 20일의 흰쥐 태자와 출생 제 1일의 신생 흰쥐 폐조직에서는 혈관, 기관지 및 폐포의 기저막과 폐포막에서 강한 laminin 면역활성이 지속되었고, 폐포가 형성된 출생 제3일 이후의 흰쥐 폐의 폐포막에서는 laminin 면역활성이 현저히 감소되었다. 2. 흰쥐 태자의 폐조직에서 laminin 면역금과립이 관찰되는 세포는 간엽세포, 혈관내피세포 및 섬유모세포였으나, 신생 흰쥐의 폐에서는 섬유모세포, 제 1형 및 제 2형 폐포세포에서 laminin 면역금과립이 관찰되었으며 금과립이 가장 많이 관찰되는 조직 부위는 공기혈관장벽을 이루는 기저 막이었다. 이상과 같은 실험결과는 태생기에서는 laminin이 주로 폐의 세포외기질에 분포되나 출생 후에는 주로 기저막에 분포되므로 패의 기능이 성숙됨에 따라 laminin의 분포상태에 변동이 일어나고, laminin을 생성하는 세포도 출생 전에는 간엽세포, 혈관내피세포 및 섬유모세포이나 출생 후에는 폐의 실질세포인 제 1형 폐포세포와 제 2형 폐포세포로 합성기능이 이전되는 것으로 생각되었다. Laminin, a kind of multidomain glycoproteins, is mainly localized in the basement membranes of various tissues. It is known that laminin plays an important part in mammalian lung morphogenesis. The authors have undertaken this study to investigate the changes in the distribution of laminin, and to find out cells which synthesize laminin during the organogenesis and differentiation of the lung. The fetal and neoantal rats (Sprague-Dawley strain) were used as experimental animals. The immunohisto-chemical methods were employed for detection of laminin within the developing lung tissue and the immunegold cytochemical methods were performed for detection of cells which synthesize laminin according to each stage of development. The results are as follows; 1. During fetal life, strong immunoreactivity for laminin is maintained in the basement membranes of the blood vessels and the bronchioles, the extracellular matrix of the mesenchyme, and basal lamina of the alveolar septum in the fetal rat lung. 2. After birth, laminin immunoreactivity at the alveolar septum is gradually reduced. 3. During fetal life, laminin is mainly detected within the cytoplasm of the mesenchymal cells, the endothelial cells of blood vessels and the fibroblasts in fetal rat lung. 4. According to the differentiation of type I and type II pneumocyte after birth, laminin is detected within cytoplasm of the type I pneumocytes, type II pneumocytes and fibroblasts. It is consequently suggested that laminin is largely expressed in the developing lung and laminin may be also synthesized by the type II pneumonocytes at early newborn stages.

난소절제 및 에스트로겐 투여가 흰쥐 뇌의 β-endorphin 면역활성도에 미치는 영향

정호삼,황세진,문영진,이재억,유중배,황윤영,조수현 대한폐경학회 1995 대한폐경학회지 Vol.1 No.1

The relationship between menopause and effective disorders have not been well known, But there is ample evidence for action of estrogen on the dopaminergic cholinergic, Gamma Aminobutyric Acid and various neuropeptide systems. We try to ealuate the effects of oophorectomy and estrogen replacement therapy on β-endorphin immunoreactivity. Sprague-Dawley rats, weighing 250-300gm were devide into sham operation, bilateral oophoretomy and bilateral oopharectomy with estradiol cypionate(10㎍/㎏) treatment group. After one month, brain was removed and processed for β-endorphin immunostain with Avidin-Biotin method. Β-Endorphin immunostaining in both medial basal hypothalamus and nucleus accumbens were decreased in oophorectomized rats compared to those of sham operation. In estradiol treated rats, endorphine immunostaining in medial basal hypothalamus was decreased but sama as sham operation in Nucleus accumbens. These results suggest that surgical menopause causes the decrease of β-endorphin immunoreactivity while estrogen replacement prevent the decrease of β-endorphin immunoreactivity in nucleus accumbens. These findings may partly explaining changes in affective state in some women after menopause.

[지상33]비타민 C가 Adriamycin을 투여한 흰쥐 심장근육에서 NF-κB, AP-1, Bcl-2 Bax 와 의 분포에 미치는 영향

정호삼,조용범,백두진 대한체질인류학회 2004 대한체질인류학회 학술대회 연제 초록 Vol.47 No.-

韓國産 자라(Amyda sinensis)의 脾臟組織에 關하여

정호삼,이경로,Chung, Ho Sam,Lee, Kyung Ro The Korean Society for Integrative Biology 1975 동물학회지 Vol.18 No.3

1) 자라의 비장을 싸고있는 皮膜은 筋內細胞가 없으며 哺乳類의 그것과 마찬가지로 망상섬유와 탄력섬유 및 교원섬유를 함유하고 있다. 2) 자라의 비장의 脾株는 개구리 비장보다 발달되고 짧은 脾株가 잘 발달되어 있다. 3) 자라 비장의 血管系는 哺乳類와 거의 비슷하나 특히 中心動脈이 잘 발달되고 脾洞의 발달은 포유류의 脾洞構造나 數量面에서 下等性을 보인다. 4) 자라 비장의 造血細胞의 출현빈도가 포유류와 양서류등 다른 脊椎動物보다 훨씬 많이 出現된다. Histological studies on the spleen of mud turtle(Amyda sinensis) in Korea were made and the results obtained were as follows: 1. Smooth muscle cells are absent in the capsule of the spleen, although reticular, elastic and collagenous fibers are presnet as in the spleen of other mammals. 2. The trabeculs of the spleen tissue are more highly developed than those of frog and do not extend as deeply into the splenic pulp as the trabecula of mammals. 3. The structure of blood system in the spleen is found to be similar to that of mammals. Particularly, the central arteries of spleen are more highly developed, but the splenic sinus appears to be less developed than that of mammals. 4. The ratio of red pulp and white pulp in the splenic pulp appears to be 1. 5. More hemopoietic cells are seen in the spleen of turtle than in the spleen of mammals.

[지상27]Adriamycin 투여후 흰쥐 왼심실근육층에서 세포자멸사의 발현에 미치는 Vitamin C의 영향

정호삼,서윤경,백두진 대한체질인류학회 2003 대한체질인류학회 학술대회 연제 초록 Vol.46 No.-

Retinoic acid가 흰쥐태자의 허근육의 분화와 Laminin 합성에 미치는 영향

정호삼,김원규,황규성,서윤경,윤지회,백두진,김혜주 대한체질인류학회 2000 대한체질인류학회 학술대회 연제 초록 Vol.43 No.-

흰쥐의 허파분화 과정에서 Laminin 활성의 변화에 대한 면역조직화학적 및 면역도금법에 의한 연구

정호삼,서윤경,김원규,윤지희,백두진,박철홍 대한체질인류학회 2000 대한체질인류학회 학술대회 연제 초록 Vol.43 No.-

Interleukin-2가 Mouse 비장의 혈구전구체세포에 미치는 영향에 관한 전자현미경적 연구

정호삼 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2

Interleukin-2, T-cell growth factor, is a glycoprotein produced by the lymphocytes and it is widely used as an immunotherapeutic agent on the hepatoma and various carcinoma. Interleukin-2 mediate antitumor effect by stimulating the proliferation of helper and cytotoxic T-cells and increasing the generation of lymphokine activated killer cells. Thus interleukin-2 was administered to the cancer patients and experimental animals, it could also effect on the lymphoblasts and reticular cells in the spleen. In this experiment, the author pursued the effect of interleukin-2 on the ultrastructure of the reticular cell of the mouse spleen. Albino mice, DDY strain, weighing 20gm were used as experimental animals. The experimental animals were killed at 3, 6, 12, 24 and 48 hours after administration of 2,000,000 unit/kg interleukin-2. The specimens obtained from the spleen were prefixed in 2% glutaraldehyde-2.5% paraformaldehyde solution and postfixed in the 1% osmic acid. After dehydration, the specimen were embedded in Epon 812 and then ultrathin sections(600-800A??) were made and stained with urarnyl acetate and lead nitrate. And these preparations were observed with JEM 100cx-II electron microscope. The results were as follows. 1. Medium sized lymphocytes, large lymphocytes and reticular cells were increased in the white pulp of the 3, 6 and 12 hours interleukin-2 treated mouse spleen. A few rod shaped rough endoplasmic reticulum were observed and mitochondria and vesicles were abundant in its cytoplasm. 2. Small lymphocytes, medium sized lymphocytes, large lymphocytes and reticular cells were seen as regular shapes and their cytoplasmic organelles were normal in the white pulp of the spleen in 24 and 48 hour interleukin-2 treated Mice group. Consequently, it is suggested that interleukin-2 would induce the development of the cytoplasmic organelles in the reticular cells but the cytoplasmic organelles in the reticular cells would return to its normal state as the time goes by.

Melanoston의 탈색효과에 대한 면역조직화학적 및 전자현미경적 연구

정호삼,오철,강병찬,정호권 대한체질인류학회 2003 대한체질인류학회지 Vol.16 No.4

Immunohistochemical and Electron Microscopic Study on the Depigmentation Effect of MelanostonHo-Sam Chung, Chul Oh1, Byung-Chan Kang1, Ho-Kwon Jung1