허혈성 심질환에 있어서 아데노신 급속 정맥투여후 관동맥압의 변화에 관한 연구

봉종대,오종용,배성한,신원용,김철현,이광희,최태명,현민수,김성구,권영주 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Background: Adenosine interacts with A1 receptors present on the extracellular surface of cardiac cells, activating K+channels in a fashion. It produces coronary vasodilatation and AV nodal block. The half-time is one to six second and has ultra-short action. In ischemic heart disease, fractional flow reserve(FFR) can be achieved safely with intravenous adenosine infusions at a rate of 150㎍/kg/min which cause maximal coronary hyperemia. Purpose: We investigated the patients who have had ischemic heart disease(n=8). After adenosine intravenous administration(150㎍/kg/min), the effect cause maximal hyperemia of coronary artery. During peak maximal hyperemia, we reported clinical findings, coronary hemodynamics and electrocardiogram findings. Methods: After diagnostic coronary angiography and left ventriculography, catheter was advanced into the ostium of coronary artery. We investigated coronary hemodynamics such as systolic coronary artery pressure, diastolic coronary artery pressure, mean coronary artery pressure and heart rate. To assess the use of adenosine for determination of fractional flow reserve, hemodynamics and electrocardiogram were measured at basal state and during peak maximal hyperemia. Results: Symptoms developed 1.5-2 minute after adenosine intravenous administration and each symptom disappeared 2-2.5 minute after adenosine administration. Two patients represented asymptomatic appearance and six patients complained of flushing, chest pain, palpitation and headache. In eight patients with ischemic heart disease, 1.5 minute after venous administration of 150㎍/kg/min of adenosine, coronary artery pressure decreased and heart rate increased significantly. Conduction abnormalities after administration of 150㎍/kg/min of adenosine were transient 2nd degree AV block 2 cases(25%). Conclusions: Adenosine is a potent ultra short-acting vasodilator. Although coronary artery pressure decreased and heart rate increased and mild cardiovascular symptoms occurred after continuous infusion of 150㎍/kg/min of adenosine, it is useful as an agent for determination of fractional flow reserve.

사료원료 공급량 및 스크류 회전속도를 달리하여 제조한 배합사료가 넙치(Paralichthys olivaceus)의 성장 및 체조성에 미치는 영향

김경덕 ( Kyoung Duck Kim ),김강웅 ( Kang Woong Kim ),이봉주 ( Bong Joo Lee ),배기민 ( Ki Min Bae ),안철민 ( Cheul Min An ),한현섭 ( Hyun Sob Han ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5

The aim of this study was to investigate the effect of diet extruder conditions, such as feed-loading rate and screw speed, on growth performance and biochemical responses in olive flounder Paralichthys olivaceus. Over 8 wks, we used four identical diets (triplicated per treatment) with differing ratios of feed-loading rate (kg/h):screw speed (rpm/min) in a laboratory-scaled twin-screw extruder of 50:640, 80:640, 120:640, and 80:400, designated as EP1, EP2, EP3, and EP4, respectively. Screw speed impacted the buoyancy of experimental diets. Diets produced at a screw speed of 640 rpm/min floated for > 24 hrs, whereas those produced at a speed of 400 rpm/min sank between 10 s and 5 min. Fish that were fed EP1 and EP4 diets grew significantly faster than those fed EP2 and EP3 diets. Fish fed EP1 diets ate and gained weight most efficiently among treatments, a result that is likely to be related to feed-loading rate, i.e., ingredients extruded at a low feed-loading rate may have more time to cook in the pre-conditioner of the extruder. A cooked diet may be easier to digest in fish. Fish fed EP4 diets also showed significant weight gain, as compared to those fed EP2 and EP3 diets. However, we found no differences among treatments in proximate compositions of dorsal muscle, liver, and viscera of fish. Our results suggest that extruder conditions, may influence feed quality, impacting feed efficiency and growth of fish.

급속열처리 장비의 온도 조절기용 전력 제어장치의 설계

주동만,배민호,민경일 永同大學校 2000 硏究論叢 Vol.6 No.1

위상 제어방식을 이용한 급속 열처리 장비의 온도조절기용 전력 제어장치를 설계하는 방법을 제시한다. 본 논문에서 제시된 설계 방법에 따라 전력제어장치를 제작 · 실험한 결과 가변입력제어신호(0~10 V)에 대해 가변출력평균전압(0~198.06 V), 제어 분해능 48.356 mV(12bit)를 얻었다. The method of designing power controller for temperature control of rapid thermal processing by using phase control method is presented. The power controller is fabricated by using design method presented is this method. The experimental results show that variable output average voltages are 0∼ 198.06 V and control resolution is 48.356 mV(12bit) at variable input control signal voltage range(0~ 10 V)

고온 초전도체의 국부�치 에너지에 따른 온도분포 해석

주민석,배준한,고태국,강연일 연세대학교 산업기술연구소 1994 논문집 Vol.26 No.1

The transient one-dimensional heat conduction equation in a high Tc bulk superconductor with an localized hot zone has been analyzed numerically. After the heater energy is introduced in a few miliseconds, the development of the normal zone and full quench time are calculated with respect to distance as a fuction of three parameters including : transport current, heater current, heating time. The analysis of this model is performed using finite difference method under modified adiabatic condition. The results of computer simulation are also presented in this paper. Full quench occurs when transport currents exceed 1.15[A] in given heater current and heating time. A temperature of superconductor rises abruptly in heating region when heater energy is increased. If heater energy is same, heating time is dominant factor for quench. Given heater energy, ohmic heat dissipation is dominant part for quench. But, heating energy is dominant part for quench than conduction. When heater energy is large, quench is not affected by transport currents.

초전도 코일의 �치 시뮬레이션 해석

배준한,주민석,고태국 연세대학교 대학원 1994 延世論叢 Vol.30 No.1

This thesis is a study on the quench characteristics in epoxy impregnated superconducting magnet. Teh normal 3one propagation velocity, full quench time and full recovery time in a superconducting magnet during quench are simulated numerically. Propagation velocity is linearly proportional to the transport current and abruptly increases near the critical current. Longitudinal velocity is nearly 20 times of transverse velocity regardless of the transport current. The ratio of the transverse velocity to longitudinal velocity is about 0.04 regardless of the transport current and the full quench time is abruptly decreased near the minimum propagation current. Comparing superconducting composites composed of Cu matrix to the composites of Cu/CuNi matrix, the propagation velocity of the former is slower than the latter, while the full quench time of the former is longer under the same transport curent.

설악산 국립공원의 어류분포

장민호,조가익,하진용,정광석,박성배,주기재 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.4

설악산 국립공원지역 산간계류의 17개 지점에서 2000년 6월부터 2001년 6월까지 어류상 및 이화학적 요인을 계절별로 총 4회에 걸쳐 분석하였다. 모든 조사지점은 1~3차의 하천으로 비교적 물리·화학적 교란이 적었으며 하천폭은 5~30 m 정도였다. 채집된 어류는 총 18과 42종 2,557개체였고, Cyprinidae는 16종으로 84.7%의 상대풍부도를 나타냈으며, Gobiidae 4종, 5.0?의 상대풍부도를 보였다. Cyprinidae는 전체 채입종의 37.2%를 나타냈다. 우점종은 Zacco temminck로 10개 지점에서 738개체가 채집되어 28.9%의 상대풍부도를 나타냈으며, 아우점종은 Rhynchocypris kumkangensis와 Z. platypus로 각각 4개 지점 648개체(25.3%)와 7개 지점 344개체(13.5%)로 나타났다. 한국고유종(Korean endemic species)은 모두 4과 11종 781개체로 30.5%의 상대풍부도를 나타냈으며, 고유화 빈도는 25.6%로 한국의 평균 고유화 빈도와 유사하게 나타났다. 본 조사에서 외래어는 채집되지 않았다. 국립공원 내에 서식하는 어류의 다양성 유지와 회복을 위해서는 공원 내는 물론 경계지역 하천의 체계적인 관리가 요구된다. The fish fauna of mountain streams in the Seorak National Park area was investigated from June 2000 to June 2001 (4 times). All 17 sites were relatively undisturbed 1st to 3rd order streams. A total of 2,557 individuals were collected and classified into 17 families and 43 species. Cyprinidae accounted for 37.2% (16 species) of the total fish species and 84.7% (2,165 ind.) of the total abundance. Subdominant families were Gobiidae (4 species, 5.0%) and Balitoridae (3 species, 3.4%). Zacco temmincki (RA 28.9%, 10 sites) dominated. Subdominant species were Rhynchocypris kumkangensis (25.3%, 4 sites) and Zacco platypus (13.5%, 7 sites). Eleven of the species are endemic to Korea (4 families, 30.5%). Exotic species were not collected in this survey. Fish diversity was higher in streams around the boundary area than in the national park. Therefore, management of streams in the boundary area to protect the fish biodiversity can be efficiently conducted. Habitat degradation should be reduced for the conservation and restoration of fish diversity.

흰쥐 뇌에서 전기경련 충격에 의한 CREB 인산화의 발달단계에 따른 변화

강웅구,정희연,안용민,정선주,전송희,박주배,조수철,김용식 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적 : 이 논문에서는 뇌에서 전기경련 충격(Electroconvulsive shock. ECS)에 의한 전사인자 CREB의 인산화를 발달단계에 따라 알아봄으로써 정신장애의 신경발달학적 이해를 위한 생물학적 기반지식을 얻고자 하였다. 방 법 : 생후 7. 14. 21일 및 성년 흰쥐에 ECS를 가하고 시간별로 해마 및 소뇌 조직을 얻어 CREB의 인산화를 알아보기 위해 특이 항체로 면역블롯을 실시하였다. 결 과 : 발달단계에 따라 해마에서는 CREB이 감소한 반면 소뇌에서는 CREB이 증가하였다. 기저상태의 CREB 인산화는 해마와 소뇌에서 생후 7일에 비해 14일 이후 증가하였는데, 소뇌의 경우 CREB의 양증가와 비례하였다. ECS 후 CREB인산화 증가는 해마에서는 생후 21일 이후에 나타났으나, 소뇌에서는 생후 7일은 물론 성년에서도 나타나지 않았다. 결 론 : CREB 매개 신호전달은 발달단계별 및 조직별 활성의 차이를 나타내었으며 해마에서는 생후 21일 이후 ECS에 의해 활성화되었지만, 소뇌에서는 그렇지 않았다. 해마에서 ECS에 의한 CREB 인산화 증가는 c-fos 유전자의 발현과 관계있으리라고 생각되지만, CREB의 Ser-133 인산화 만으로는 발달단계 및 조직에 따른 c-fos 발현의 특이성을 설명할 수 없었다. Objectives : In order to understand the biological basis of neurodevelopmental perspectives of mental disorders, the authors investigated the developmental and regional changes in the phosphorylation of the transcription factor CREB following the electroconvulsive shock(ECS) in rat brain. Methods : Rats of various age groups(7, 14, 21 days postnatal and adults) were given ECS and their hippocampi and cerebella were dissected at specified time points. The content of CREB and phosphorylated CREB were measured by immunoblot analysis. Results : The amount of CREB increased in the hippocampus and decreased in the cerebellum according to the age. Baseline levels of CREB phosphorylation in both tissues were increased from postnatal 14 days, and it was proportional to the amount of CREB protein in the cerebellum. In the hippocampus, ECS increased the phosphorylation of CREB at postnatal 21 days, but in the cerebellum, ECS did not increased the phosphorylation of CREB in any age group. Conclusion : CREB mediated signal transduction pathways showed developmental and tissue-specific changes. ECS increased the phosphorylation of CREB in the hippocampus by postnatal 21 days, but not in the cerebellum. CREB activation is supposed to be related with the induction of c-fos after ECS in the hippocampus. However, the Ser-133 phosphorylation of CREB could not completely explain the developmental and tissue specificity of c-fos induction.

흰쥐 해마에서 경련에 의해 발현 유도된 MKP-1에 의한 MAPK 활성 조절

유범희,강웅구,안용민,정선주,전송희,박주배,김용식 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.4

연구목적 : 전기경련 충격(Electroconvulsive shock, ECS) 및 카이닌산(kainic acid)에 의한 경련은 흰쥐 해마에서 mitogen activated protein kinase(MAPK)를 활성화시키며, 동시에 MAPK 불활성화 효소인 MAPK phosphatase-1(MKP-1)의 발현을 일으킨다. 이 연구의 목적은 경련에 의해 발현된 MKP-1이 역시 경련에 의해 활성화된 MAPK의 불활성화에 관여하는지를 알아보고자 하는 것이다. 방 법 : 흰쥐에 ECS를 가하여 해마에서 MKP-1 발현을 일으킨 뒤 다시 ECS를 가하고, 두 번째 ECS에 의한 MAPK의 일시적 활성화가 MKP-1의 발현상태에 의해 영향받는지를 알아보았다. 또한 흰쥐에 지속적인 MAPK 활성화 및 MKP-1 발현을 일으키는 카이닌산을 투여한 뒤 해마에서 MKP-1의 발현과 MAPK 활성과의 관계를 알아보았다. 결 과 : ECS후 해마에서 타이로신 인산화 면역블롯 및 효소활성으로 측정한 MAPK의 인산화 및 활성은 MKP-1의 발현이 일어나 있는 경우와 그렇지 않은 경우 사이에 차이를 보이지 않았다. 카이닌산의 투여에 의해 MAPK 활성화가 일어나는 경우, 뒤이어 MKP-1 발현이 일어나지만, 이렇게 발현된 MKP-1은 MAPK 활성을 충분히 감소시키지 못하였다. 결 론 : MKP-1은 흰쥐 해마에서 ECS 및 카이닌산에 의한 MAPK의 활성화를 차단하는데 큰 역할을 하지 않는다. Objectives : Both electroconvulsive shock(ECS)- and kainic acid-induced seizures activate mitogen-activated protein kinases(MAPKs) in rat hippocampus. They can also induce the expression of MAPK phosphatase-1(MKP-1) in rat hippocampus. MKP-1 is known as a specific MAPK deactivator. This study aimed to elucidate the role of MKP-1 in the deactivation of MAPKs in rat hippocampus. Methods : In order to induce MKP-1 in the hippocampus, ECS was given to the rats. At the time points when MKP-1 was sufficiently induced, the second ECS was given to them and the subsequent phosphorylation or activation of MAPKs were measured in the hippocampus. A second group of rats were injected with kainic acid and the relationship between MKP-1 expression and MAPK phosphorylation was examined in their hippocampi. Results : The expression of MKP-1 did not influence the phosphorylation or activation of MAPKs following ECS in rat hippocampus. Kainic acid-induced expression of MKP-1 did not significantly reduce the phosphorylation of MAPKs. Conclusion : MKP-1 did not play a significant role in the deactivation of MAPKs which were activated by ECS or kainic acid in rat hippocampus.

신이식에서 이식신의 생존분석

오선미,김종학,황평주,구영선,강민규,나기량,김종섭,김성숙,이강욱,신영태,설종구,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

To investigate the prognostic factors for the survival of transplanted kidney in patients with end-stage renal failure, 59 cases of renal transplantation from September 1986 to Feburary 1997 in Chungnam National University Hospital were analyzed retrospectively. The results were as follows: 1)The mean age of recipients was 33.8 years and that of doners was 38.9 years. The male to female ratio of recipients was 2.5:1, and that of donors was 1.03:1 2) Living related donore(LRD) were 79.6% and living non-related donors were 20.3%. The HLA-identical donors(ID) in LRD were 18.7% and HLA-haploidentical donors(HID) were 61.0%. In living non-related donors(LNRD), mean matched HLA-AB antigens were 1.56 and mean matched HLA-DR antigens were 0.56. 3) The average 5-year patient survival fate was 94%, and average 5-year graft survival rate was 70%. The 5-year graft survival fate of HLA-ID was 100%, and those HLA-HID and LNR were 70% and 36% respectively. 4) Total 33 episodes of acute rejection were found in 45.8% of transplanted patients. The number of acute rejection episode did not show significant difference between LRD and LNRD(P > 0.05). There was no significant difference in MLC between two groups(p > 0.05). 5) In comparison between the 18 patients who lost their graft function in 5 years and 17 patients who are maintaining graft function for more than 5 years, MLC was significantly lower in patients with functioning graft than of patients with non-functioning graft (p < 0.05). The number of rejection episode was also lower in patients with functioning graft than that of the patients with non-functioning graft(p < 0.05). However, there were no significant difference in recepient and donor age and history of pre-transplantation donor specific transfusion between two groups. With the results above, we can speculate that adequate donor selection according to good matched HLA typing and low MLC is very important for graft survival in renal transplantation. Prevention and treatment of acute rejection in renal transplantation is also na important factor for graft survival.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.