http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
넙치( Paralichthys olivaceus ) 치어 사료에서 산 가수분해 농축대두박 (Acid-concentrated soybean meal)의 어분대체효과 및 소화율 평가
김성삼 ( Sung Sam Kim ),오대한 ( Dae Han Oh ),조성준 ( Seong Jun Cho ),서상현 ( Sang Hyun Seo ),한현섭 ( Hyon Sob Han ),이경준 ( Kyeong Jun Lee ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.6
This study was conducted to investigate the effects of fishmeal replacement with acid-concentrated soybean meal (ACSBM) on growth performance, blood biochemistry, and ingredient digestibility in juvenile olive flounder Para lichthys olivaceus . Six experimental diets were formulated to replace fishmeal protein with ACSBM at 0%, 20%, 30%, 40%, 50%, and 60% (designated ACSBM0, ACSBM20, ACSBM30, ACSBM40, ACSBM50, and ACSBM60, respectively). Triplicate groups of fish (initial fish mean weight: 14.3±0.03 g) were fed the experimental diets to apparent satiation (twice daily at 08:00 and 18:00 h). After a 12-week feeding trial, a total of 180 healthy fish were randomly distributed into three Guelph system tanks at a density of 60 fish/tank (initial fish mean weight : 50.6±2.4 g) to test the apparent digestibility coefficients of the ingredients (ACSBM, fishmeal, and soybean meal). Although negative effects were observed with ACSBM40, ACSBM50 and ACSBM60 after 12 weeks of feeding, up to 20% of the fishmeal protein could be successfully replaced with ACSBM without significant growth depression. Hemoglobin and hematocrit values of fish fed the ACSBM50 and ACSBM60 diets were significantly lower than those of fish fed the ACSBM0 diet. Glucose values of fish fed the ACSBM60 diet were significantly higher than those of fish fed the ACSBM0 and ACSBM20 diets. Digestibility of protein in ACSBM and soybean meal was 85.9% and 82.5%, respectively. Results indicated that at least 20% of fishmeal protein can be replaced by ACSBM in diets of juvenile olive flounder without supplementation of limiting amino acids.
박은미,조현국,홍경은,남보혜,김영옥,김우진,이상준,한현섭,이재용,김종식,장인권,정재훈,최태진,공희정,Park, Eun-Mi,Cho, Hyun Kook,Hong, Gyeong-Eun,Nam, Bo-Hye,Kim, Young-Ok,Kim, Woo-Jin,Lee, Sang-Jun,Han, Hyon Sob,Lee, Jae Yong,Kim, Jong-Sheek,Ja 한국해양바이오학회 2007 한국해양바이오학회지 Vol.2 No.1
Penaeidins은 새우류에 존재하는 항미생물성펩티드의 한 종류로서 새우의 외부 병원체에 대한 방어기작을 구성하는 중요한 인자이다. 본 연구에서는 대하 혈구세포의 cDNA library로부터 분리된 EST 클론을 분리 동정하였다. 아미노산 염기서열 분석과 계통수 분석을 통하여 본 연구에서 분리한 EST 클론이 대하의 Penaeidin 3-2 유전자와 아미노산 수준에서 동일함을 밝혔다. 대하의 Penaeidin 3-2는 signal peptides로 예상되는 19개 아미노산 잔기를 포함하여 전체 71개의 아미노산 잔기로 이루어져 있고, C-말단에는 3개의 이중황화결합을 형성할 수 있는 6개의 cysteine가 존재하였다. 대하 Penaeidin 3-2 전사체는 혈구세포, 간췌장, 근육 조직에서 발현되었으며, 특히 혈구세포에서 주로 많이 발현되었다. 흰반점바이러스의 인위감염 실험에서 바이러스 감염 후 대하 Penaeidin 3-2의 발현이 증가하였다. 이상의 결과들로부터 대하 Penaeidin 3-2가 자체 방어 작용에서 중요한 역할을 할 것으로 예상된다. Penaeidins are members of a special family of antimicrobial peptides existing in several species of shrimp and play a crucial role in the immunological defense of shrimp. In this study, we isolated and characterized one EST clone (penaeidin) from cDNA library of fleshy prawn Fenneropenaeus chinensis hemocytes. Amino acids sequence comparison and phylogenetic analysis with other known penaeidins revealed that our clone was completely identical to F. chinensis Penaeidin 3-2 (Accession no. ABC33920), which composed of 71 amino acids with a putative signal peptide (1-19) and a cysteine-rich domain (C-terminal part). The expression and distribution of Penaeidin 3-2 transcripts in shrimp were detected in hemocytes, hepatopancreas, and muscles, and that Penaeidin 3-2 was constitutively expressed mainly in hemocytes. The artificial infection of white spot syndrome virus to F. chinensis resulted in Penaeidin 3-2 mRNA up-regulation in hemocytes, suggesting that the possible role of Penaeidin 3-2 in host defense system of F. chinensis.
수온별 조피볼락(Sebastes schlegeli) 치어의배합사료 적정 공급횟수
이진혁 ( Jin Hyeok Lee ),이봉주 ( Bong Joo Lee ),김강웅 ( Kang Woong Kim ),한현섭 ( Hyon Sob Han ),박건현 ( Gun Hyun Park ),이준호 ( Jun Ho Lee ),윤현호 ( Hyeon Ho Yun ),배승철 ( Sung Chul Bai ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.6
We conducted two feeding trials to investigate the optimal feeding frequency of juvenile Korean rockfish Sebastes schlegeli fed a commercial diet of expanded pellets containing 47.2% crude protein, 9.2% crude lipid, and 14.5% ash at two different water temperatures. In the first experiment, triplicate groups of 20 fish with an average weight of 2 g were fed an equal amount of diet (5.97%based on body weight) at one of six feeding frequencies (2, 3, 4, 5, 7, or 9 meals/day) for four weeks at 17.5˚C. After four weeks,we measured weight gain (WG), specific growth rate (SGR), feeding efficiency (FE), and protein efficiency ratio (PER). Fish fed diet five meals/day grew significantly better than those fed nine meals/day. The second experiment used identical experimental conditions and feeding regions, except the food ration was slightly less (5.92% based on body weight) and the water temperature was increased to 20˚C. After four weeks, we again measured WG, SGR, FE and PER. Fish fed seven meals/day grew significantly faster than those fed 2, 3, 4, or 9 meals/day. Whole-body protein levels in fish fed three meals/day was higher than those fed four meals/day in 17.5˚C water, but whole-body lipids in the fish fed four meals/day was higher than those fed two meals/day in 20˚C water. A second-order polynomial analysis based on WG suggested the optimal feeding frequency for juvenile Korean rockfish was five meals/day at 17.5˚C and six meals/day at 20˚C, indicating that fish reared in higher water temperature require higher feeding frequencies.
해상가두리 양식장에서 배합사료 및 생사료 공급에 따른 조피볼락 (Sebastes schlegeli)의 성장 및 육질 비교
이봉주 ( Bong Joo Lee ),김신권 ( Shin Kwon Kim ),김강웅 ( Kang Woong Kim ),김경덕 ( Kyoung Duck Kim ),한현섭 ( Hyon Sob Han ),손매현 ( Maeng Hyun Son ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.3
This study was conducted to compare extruded pellets (EP) and soft extruded pellets (SEP) with a raw fish-based moist pellet (MP) diet on the growth and flesh quality of Korean rockfish Sebastes schlegeli. Three groups of 20,000 fish (initial mean weight 133 g) per net-cage (6×12×7 m) were fed commercial EP, SEP or MP for 16 months. The survival of fish fed SEP was higher than those of fish fed EP or MP. The highest growth performances were observed in the mean weight gain, total weight gain, and feed efficiency of fish fed MP, followed by those fed EP and SEP. Among the fish fed on extruded pellets, the total weight gain of fish fed SEP was higher than that of those fed EP, while fish fed EP grew faster than those fed SEP. No notable differences in body composition, sensory scores or textural properties of the dorsal muscle were observed in fish fed on EP,SEP or MP. Thus, it is suggested that extruded pellets, rather than raw fish-based moist pellets, could be fed to Korean rockfish without compromise-ing flesh quality.
배합사료의 우지와 덱스트린이 메기(Siurus asotus)의 성장과 체조성에 미치는 영향
김진도 ( Jin Do Kim ),김강웅 ( Kang Woong Kim ),김경덕 ( Kyoung Duck Kim ),손맹현 ( Maeng Hyun Son ),한현섭 ( Hyon Sob Han ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.3
This study investigated the utilization of beef tallow and dextrin as non-protein energy sources in the diet of juvenile far eastern catfish Silurus asotus. Three replicated groups of juveniles (initial mean weight of 3.6 g) were fed diets containing high levels of beef tallow or dextrin for six weeks. The final mean weight of fish fed on a 17% lipid diet with a high level of beef tallow was significantly higher than that of fish fed on a 9% lipid diet with a high level of dextrin (P=0.04). No significant differences were observed in the feed efficiency and protein efficiency ration of fish fed on the experimental diets. Crude lipid content and the C18:0, 1n-9 and C18:2n-6 contents of whole-body fish were significantly affected by diet (P<0.05). The results of this study suggest that beef tallow is better than dextrin as a non-protein energy source for improving the growth of juvenile far eastern catfish, and that beef tallow could be economically used as an ingredient in practical feed.
사료원료 공급량 및 스크류 회전속도를 달리하여 제조한 배합사료가 넙치(Paralichthys olivaceus)의 성장 및 체조성에 미치는 영향
김경덕 ( Kyoung Duck Kim ),김강웅 ( Kang Woong Kim ),이봉주 ( Bong Joo Lee ),배기민 ( Ki Min Bae ),안철민 ( Cheul Min An ),한현섭 ( Hyun Sob Han ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5
The aim of this study was to investigate the effect of diet extruder conditions, such as feed-loading rate and screw speed, on growth performance and biochemical responses in olive flounder Paralichthys olivaceus. Over 8 wks, we used four identical diets (triplicated per treatment) with differing ratios of feed-loading rate (kg/h):screw speed (rpm/min) in a laboratory-scaled twin-screw extruder of 50:640, 80:640, 120:640, and 80:400, designated as EP1, EP2, EP3, and EP4, respectively. Screw speed impacted the buoyancy of experimental diets. Diets produced at a screw speed of 640 rpm/min floated for > 24 hrs, whereas those produced at a speed of 400 rpm/min sank between 10 s and 5 min. Fish that were fed EP1 and EP4 diets grew significantly faster than those fed EP2 and EP3 diets. Fish fed EP1 diets ate and gained weight most efficiently among treatments, a result that is likely to be related to feed-loading rate, i.e., ingredients extruded at a low feed-loading rate may have more time to cook in the pre-conditioner of the extruder. A cooked diet may be easier to digest in fish. Fish fed EP4 diets also showed significant weight gain, as compared to those fed EP2 and EP3 diets. However, we found no differences among treatments in proximate compositions of dorsal muscle, liver, and viscera of fish. Our results suggest that extruder conditions, may influence feed quality, impacting feed efficiency and growth of fish.