An S-locus receptor-like kinase in plasma membrane interacts with calmodulin in <i>Arabidopsis</i>

Kim, Ho Soo,Jung, Mi Soon,Lee, Kyunghee,Kim, Kyung Eun,Yoo, Jae Hyuk,Kim, Min Chul,Kim, Doh Hoon,Cho, Moo Je,Chung, Woo Sik Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca<SUP>2+</SUP>-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.</P><P><B>Structured summary</B></P><P>MINT-6800947:<I>CBRLK1</I> (uniprotkb:Q9ZT06) and <I>AtCaM2</I> (uniprotkb:P25069) <I>bind</I> (MI:0407) by <I>electrophoretic mobility shift assay</I> (MI:0413)</P><P>MINT-6800966:<I>AtCaM2</I> (uniprotkb:P25069) and <I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>bind</I> (MI:0407) by <I>competition binding</I> (MI:0405)</P><P>MINT-6800930:<I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>binds</I> (MI:0407) to <I>AtCaM2</I> (uniprotkb:P25069) by <I>far Western blotting</I> (MI:0047)</P><P>MINT-6800978:<I>AtCaM2</I> (uniprotkb:P25069) <I>physically interacts</I> (MI:0218) with <I>CBRLK1</I> (uniprotkb:Q9ZT06) by <I>cytoplasmic complementation assay</I> (MI:0228)</P>

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

강남경희한방병원에 내원한 냉증을 호소하는 여성의 Heart Rate Variability 특성 연구

이미주 ( Mi Joo Lee ),김은경 ( Eun Kyung Kim ),이진무 ( Jin Moo Lee ),조정훈 ( Jung Hoon Cho ),장준복 ( Jun Bock Jang ),이경섭 ( Kyung Sub Lee ),이창훈 ( Chang Hoon Lee ) 대한한방부인과학회 2011 大韓韓方婦人科學會誌 Vol.24 No.3

개에서 발생한 기관 허탈의 진단과 관리

이새미,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 11-year-old Yorkshire terrier anesthetized for computed tomography. The dog showed cyanosis and syncope during postanesthetic recovery, therefore drug administrated parenterally. Goose-honking was noticed after full recovery. As a result of survey radiography and fluoroscopy, grade 4 tracheal collapse was diagnosed. Prescription for the dog was oral administration of hydrocodone 0.22 mg/kg. theophylline 20 mg/kg, prednisone 0.5 mg/kg, amoxicillin 22 mg/kg and chlorpheniramine 2 mg/kg twice a day for 3 days. A 10-year-old Pomeranian was diagnosed as grade 2 traceal collapse at the local animal hospital 2 years before being referred to the veterinary teaching hospital of Chungnam National University. On physical examination, goose-honking, coughing and intermittent open-mouth breathing. Tracheal narrowing was observed on survey einradiography and fluoroscopy. Oral administration of theophylline 13 mg/kg, enrofloxacin 5 mg/kg, chlropheniramine 0.5 mg/kg twice a day was prescribed for 2 weeks.

지루각화증과 유사한 임상양상을 보인 색소성 Bowen 병 1예

조유경,김미연,박영민,김형옥,안성열 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.3

Pigmented Bowen's disease is characterized by microscopically increased melanin pigment in the epidermis and/or papillary dermis in addition to the typical findings of Bowen's disease. Pigmented Bowen's disease is often confused clinically with pigmented lesions, such as seborrheic keratosis, melanocytic nevus, pigmented basal cell cacinoma and superficial spreading melanoma. A 74-year-old man presented with a black hyperkeratotic nodule ovrlying a solitary dark brownish patch on the scalp, this was clinically diagnosed as seborrheic keratosis. Histopathologic examination revealed pigmented Bowen's disease. We, herein, report an unusual case of pigmented Bowen's disease, which is the first reported case to have occurred on the scalp and to clinically resemble seborreic keratosis. (Korean J Dermatol 2004;42(3):381~383)

상아질 접착 후 저장기간에 따른 접착제의 접착력 변화

조영곤,반일환,유미경 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.3

본 연구는 상아질에 2단계와 단일 단계 자가 산부식 접착제를 적용하여 복합레진을 접착한 후 저장기간에 따른 미세 인장 결합강도와 접착계면의 변화를 상호 비교하기 위하여 시행하였다. 발거된 상 ·하악 대구치 48개의 교합면측 상아질을 노출시켜 12개씩 4개의 군으로 분류하였다 사용된 접착제의 종류에 따라 SE Bond 군, AdheSE 군, Adper 군, Xeno III 군으로 분류하여 접착제를 적용한 후, 동일 회사의 복합레진 을 4 mm두께로 접착시켰다. 각 군당 4개의 치아를 선택하여 증류수가 담겨진 용기에 집어넣고 37℃의 항온기에 각각 1일 15일, 30일 동안 저장한 후 시편을 제작하여 각각 미세인장 결합강도의 측정과 주사전자 현미경 관찰 하여 다음과 같은 결과를 나타내었다. 2단계 자가 산부식 접착제는 1일에서 30간의 저장기간 동안 결합강도가 점차적으로 감소하였지만 통계학적으로 유의한 차이를 나타내지 않았고, 상아질과 접착제 간에 긴밀한 접착관계를 보였다. 반면에 단일 단계자가 산부식 접착제는 1일의 저장기간에 비해 30일의 저장기간에서 통계학적인 결합강도의 감소를 나타내었으며, 상아질과 접착제 간에 넓은 간극이 관찰되었다. 따라서 본 연구에서 나타난 두 종류의 30일 동안의 접착내구성은 2단계 자가 산부식 접착제가 단일 단계 자가 산부식 접착제보다 우수한 것으로 나타났다. This study compared the microtensile bond strength (μTBS) and microscopic change of two 2-step and two 1-step self-etching adhesives to dentin according to storage times in distilled water. Occlusal dentin was exposed in 48 human molars. They were divided to four groups by different adhesives: SE Bond group (Clearfil SE Bond), AdheSE group (AdheSE). Adper group (Adper Prompt L-Pop), and Xeno group (Xeno III). Each group was stored in 37℃ distilled water for 1, 15, and 30 days. Resin-bonded specimens were sectioned into beams and subjected to μTBS testing with a crosshead speed of 1 mm/minute. For SEM observation, one specimen was selected and sectioned in each group after each stroage time. Resin-dentin interface was observed under FE-SEM. In all storage times, mean μTBS of SE group was significantly higher than those of other groups (p < 0.05). There was no significant difference between mean μTBS of SE group and AdheSE group among all storage times, but significant difference between 1- and 30-day storage in mean μTBS of Adper group and Xeno group (p > 0.05). For 1-and 15-day storage, all groups showed the close adaptation between resin-dentin interfaces. For 30-day storage, resin-dentin interfaces showed wide gap in Adper group and separate pattern in Xeno III group. Resin-bonded specimens were sectioned into beams and subjected to μTBS testing with a crosshead speed of 1 mm/minute. For SEM observation, one specimen was selected and sectioned in each group after each stroage time. Resin-dentin interface was observed under FE-SEM. In all storage times, mean μTBS of SE group was significantly higher than those of other groups (p < 0.05). There was no significant difference between mean μTBS of SE group and AdheSE group among all storage times, but significant difference between 1- and 30-day storage in mean μTBS of Adper group and Xeno group (p > 0.05). For 1-and 15-day storage, all groups showed the close adaptation between resin-dentin interfaces. For 30-day storage, resin-dentin interfaces showed wide gap in Adper group and separate pattern in Xeno III group.

광중합방식이 콤포머의 변연폐쇄에 미치는 영향

조옥환,한진순,임미경,이수종 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.1

The aim of this study was to evaluate the effect of various polymerization techniques on the microleakage of compomer restorations. Fifty extracted human premolar and molar were used and randomly divided into 5 groups. After cavity preparation, compomer (F2000) was filled according to the manufacturer's directions. All groups, except group 5, were filled using an incremental technique. Group 1was polymerized for 40 seconds at a continuous 485mW/㎠ with a VIP (Bisco, USA) light cure unit. Group 2 was polymerized for 20 seconds at a continuous 345mW/㎠ and then for 20 seconds at 645mW/㎠ with the VIP light cure unit. Group 3 was polymerized at 400mW/㎠, gradually increased to 50mW/㎠ per 10 seconds until 550mW/㎠ was reached; total 40 seconds with a Spectrum 800 (Dentsply Caulk, USA) light cure unit. Group 4 was polymerized for for 3 seconds using an incremental technique with a Flipo (LOKKi, France) light cure unit. Group 5 was polymerized for 3 seconds using a bulk fill technique with the Flipo light cure unit. The specimens were embedded with acrylic resin, and were sectioned with diamond saws in a mesiodistal direction along the longitudinal axis of the tooth so as to pass through the center of the restoration, and three surfaces (occlusal, pulpal, and gingival) were examined with SEM. The results were as follows; 1. Group 5 showed a significantly larger gaps compared to other groups on the gingival, occlusal, and pulpal walls. 2. All groups except group 5 had no statistically significant gap on the gingival, occlusal, and pulpal walls. 3. There was no significant correlation between the amount of enamel on the gingival and occlusal walls and polymerization shrinkage.

推出 分光光度法에 의한 水銀의 定量法에 關한 硏究

趙庚烈,禹美熙 대구효성 가톨릭 대학교 1988 연구논문집 Vol.36 No.1

Extraction-spectrophotometric determination of mercury(Ⅱ) was studied by associated complex formation using antipyrine, antipyretics and eosin, halogenated xanthenes. K₂SO₄was added for the promotion of complex formation and it's optimal concentration was 0.3M. Optiaml conditions for the quantitative analysis of trace of mercury(Ⅱ) were investigated. Molar ratio of Hg(Ⅱ)-antipyrine-eosin complex was presumed as 1:4:2. Linear calibration curve was obtained in the range of 6~28㎍/㎖ at 485nm. Mercury(Ⅱ) determination of this method was interfered in the presence of metals such as ??, ??,, ??, ?? and ??, halides an dthiocyanate, but the interferences of metals except ?? were masked with 0.01M-sod. tartrate.

용가시나무(Rosa maximowicziana) 뿌리의 Triterpenoid 성분 연구(Ⅱ)

趙庚烈,禹美熙,曹圭玉 대구효성가톨릭대학교 응용과학연구소 1994 응용과학연구논문집 Vol.3 No.-

Rosa maxinowicziana Regel (Rosaceae) is a deciduous, latifoliate shrub growing in most parts of Korea. The sprout of this plant has been used for food, and the fruit for treating thirst. Also, the root of this plant has been used for rheumatism in a Korean folk medicine. From the roots of this plant, three triterpenoids were isolated and the structures were es-tablished on the basis of spectral and chemical evidence. So, these compounds are 3β-hydroxylup-30-ene-28-oic acid(betulinic acid), 2α, 3α, 19α-trihydroxyurs-12-ene-28-oic acid(euscaphic acid) and 2α, 3β, 19α-trihydroxyurs-12-ene-28-oic acid(tormentic acid), respectively.

정상인에서 전기 및 자기 자극을 이용한 설 근육의 전기생리학적 평가

조은미,류종현,안성환,조경원,임건한,김진호,김후원 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.1

Background: Abnormality of lingual muscles is clinically common. But its exact clinical assessment is quite difficult. Evaluation of motor power of the lingual muscles and their neural pathway is limited because of anatomical inaccessibility and individual variation of subjective symptoms. Transcranial magnetic stimulation (TMS) of the human motor cortex is a non-invasive tool to assess the functional integrity of the fast conducting central motor pathways and electrical stimulation of lingual nerve is a conventional method of peripheral nerve conduction. Therefore both study will be helpful to understanding of pathophysiology of lingual abnormality. Until now there is no such report in Korea. This study was conducted to acquire the easier method and normal control values of Korean subjects. Method: We recorded the motor responses from the lingual muscles of 32 healthy subjects (22 men, 10 women) by focal cortical TMS with a circular coil. Motor potentials were recorded by means of surface electrodes applied on the both side of the tongue, following TMS several centimeters lateral from the vertex and parietooccipital cortex and peripheral electrical stimulation of the 12th cranial nerve at the lateral 1/3 point of the line between the midline and angle of mandible. Results: During moderate motor activation, majority of maximal motor response were obtained 4-6cm lateral to the vertex with similar latencies. And the responses from magnetic stimulation of the intracranial segment of hypoglossal nerve were discarded because of difficulty to obtain and unsatisfactory reproducibility. The latency of motor evoked potentials in right cortical stimulation was 10.08 ± 1.17 msec and in left stimulation 10.04 ± 1.32 msec. The amplitudes in right 2.15 ± 1.02, left 2.09 ± 1.11 mV. The latencies electric hypoglossal nerve stimulation were 2.42 ± 0.34 in right, 2.49 ± 0.35 msec in left side. The amplitudes were 2.90 ± 2.37 in right, 2.43 ± 1.86 mV in left side. Central conduction times as calculated by subtracting the response latencies obtained by magnetic and electric stimulation are right 7.65 ± 1.19, left 7.55 ± 1.35 msec. There was no significant difference between man and woman, Conclusion: The method may be efficient, noninvasive, painless, and easy reproduced. With more extensive application to various neurological disorders, it comes close to being an ideal clinical conduction study technique for this cranial nerve.