Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

An S-locus receptor-like kinase in plasma membrane interacts with calmodulin in <i>Arabidopsis</i>

Kim, Ho Soo,Jung, Mi Soon,Lee, Kyunghee,Kim, Kyung Eun,Yoo, Jae Hyuk,Kim, Min Chul,Kim, Doh Hoon,Cho, Moo Je,Chung, Woo Sik Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca<SUP>2+</SUP>-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.</P><P><B>Structured summary</B></P><P>MINT-6800947:<I>CBRLK1</I> (uniprotkb:Q9ZT06) and <I>AtCaM2</I> (uniprotkb:P25069) <I>bind</I> (MI:0407) by <I>electrophoretic mobility shift assay</I> (MI:0413)</P><P>MINT-6800966:<I>AtCaM2</I> (uniprotkb:P25069) and <I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>bind</I> (MI:0407) by <I>competition binding</I> (MI:0405)</P><P>MINT-6800930:<I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>binds</I> (MI:0407) to <I>AtCaM2</I> (uniprotkb:P25069) by <I>far Western blotting</I> (MI:0047)</P><P>MINT-6800978:<I>AtCaM2</I> (uniprotkb:P25069) <I>physically interacts</I> (MI:0218) with <I>CBRLK1</I> (uniprotkb:Q9ZT06) by <I>cytoplasmic complementation assay</I> (MI:0228)</P>

용가시나무 뿌리의 성분연구

조경열,우미희,김난주 대구효성가톨릭대학교 응용과학연구소 1993 응용과학연구논문집 Vol.2 No.-

Rosa maximowicziana Regel(Rosaceae) is a deciduous, latifoliate shrub growing in most parts of Korea. The sprout of this plant has been used for food, and the fruit for treating thirst. Also, the root of this plant has been used fr rheumatism in a Korean folk medicine. From the roots of this plant, sterol and its flucoside, and t재 triterpenoid saponins were isolated and the structrues were elucidated by chemical and spectroscopic methods. So, these compounds were identified as β-sitosterol, β-sitosterool-3-O-β-D-glucopyranoside, 2α, 3β, 19α-trihydroxyurs-12-ene-28-oic acid 28-O-β-D-glucopyranosyl ester(kajiichigoside F1) and 2α, 3β, 19α-trihydroxyurs-12-ene-28-oic acid 28-O-β-D-glucopyranosyl ester(rosamultin), and were encounted in this plant for the first time.

용가시나무(Rosa maximowicziana) 뿌리의 Triterpenoid 성분 연구(Ⅱ)

趙庚烈,禹美熙,曹圭玉 대구효성가톨릭대학교 응용과학연구소 1994 응용과학연구논문집 Vol.3 No.-

Rosa maxinowicziana Regel (Rosaceae) is a deciduous, latifoliate shrub growing in most parts of Korea. The sprout of this plant has been used for food, and the fruit for treating thirst. Also, the root of this plant has been used for rheumatism in a Korean folk medicine. From the roots of this plant, three triterpenoids were isolated and the structures were es-tablished on the basis of spectral and chemical evidence. So, these compounds are 3β-hydroxylup-30-ene-28-oic acid(betulinic acid), 2α, 3α, 19α-trihydroxyurs-12-ene-28-oic acid(euscaphic acid) and 2α, 3β, 19α-trihydroxyurs-12-ene-28-oic acid(tormentic acid), respectively.

장애아동들의 지역학교내 통합교육에 대한 초·중·고등학교 교사들의 태도 비교 연구

안성우,김미경 한국특수아동학회 2001 특수아동교육연구 Vol.3 No.-

본 연구는 통합교육에 대한 초ㆍ중ㆍ고등학교의 교사들의 태도에 대해 조사하였다. 우리나라에서도 1994년에 개정된 특수교육진흥법 제15조에 의해 통합교육을 장애 아동 교육 정책으로 삼고 있다. 이 새로운 정책은 장애 아동들을 일반 학교에 받을 것인 지 아닌 지 교사들이 더 이상 결정할 수 없게 하였고 일반 학교에서 장애 아동들을 교육하는 것이 선택 사항이 아니게 되었다. 이 정책은 장애 아동들의 교육 뿐만이 아니라 일반 아동들의 교육에도 영향을 미치게 되었다. 그러나 많은 학자들은 이러한 정책의 실행 성공 여부는 교육적 지원 외에도 교사들의 통합교육에 대한 태도에 달려 있다고 지적하고 있다. 따라서 교사들의 태도를 조사하는 것은 통합교육의 성공을 위한 준비 노력으로서 상당히 의의가 있다. 본 연구에서는 초등 교사와 중등 교사들의 태도를 비교하는 것을 또 다른 목적으로 하고 있다. 이는 초등학교와 중고등학교의 교육 현실이 상이한 점 때문으로 태도는 개인이 속한 환경의 영향을 받기 때문이다. 그런데 기존의 선행 연구들은 특수교사와 일반 교사간에는 많은 연구들이 수행되어 있으나 학교급간에는 많은 연구가 수행되어 있지 않다. 따라서 학교급별 교사들의 통합교육에 대한 태도를 조사하는 것은 상당히 의의가 있다. 또 본 연구에서는 교사들의 인구통계학적 변인들이 통합교육에 대한 교사들의 태도에 미치는 영향을 알아 보려고 시도하였다. 그러나 교사들의 특수교육에 대한 지식 정도가 통합교육에 대한 태도에 미치는 영향을 통제하기 위해 교사들이 방학중 특수교육 관련 연수를 이수한 직후에 태도 조사를 실시하였다. 본 연구의 결과는 교사들이 장애 아동들의 통합교육에 대해 전반적으로는 긍정적인 태도를 갖고 있는 것으로 나타났다. 그러나 학교급간에 통합교육의 효과에 대해 상이한 태도를 갖고 있었다. 초등학교 교사들이 더 호의적이었고 중고등학교 교사들이 덜 호의적이었다. 이는 중고등학교의 교육 현실을 반영하는 것이라고 생각되어진다.또 교육적 효과의 하위 영역에서는 교사들이 통합교육이 긍정적인 영향을 미칠 것이라고 생각하는 영역도 있고 아동들의 교육에 부정적인 영향을 미칠 것이라고 생각하는 영역도 있다. 교사들은 아동들의 교과 학습면을 가장 우려하고 있었다. 교사들이 우려하는 점들을 해소할 수 있는 방향으로 교육 정책이 입안되고 실행될 수 있도록 노력하여야 할 것이다. This study investigated teachers' attitudes as measured by responses of teachers from three different levels of school toward integration of children with disabilities. In 1994 a new policy which emphasized the rights of all children to an education in community based regular schools and the consequent expectation that teachers and no choice about whether they would accept children with disabilities into their classrooms had been introduced in this country. This policy has changed teaching environments in community based regular school. In this study, it was found that teachers expressed positive attitudes toward the integration of children disabilities into their classroom. It was also found that the attitudes toward mainstreaming students with disabilities of teachers in elementary schools are more positive than the attitudes of teachers working in either middle school or high school, and that teachers did not view that mainstreaming help non handicapped children in academic area. They viewed that mainstreaming might have a negative effect on an academic achievement of non handicapped students in general. However, it was pointed out that the availability of ancillary staff was seen as a major factor in the more positive attitudes toward the policy.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

인터넷 상점에서 개인화 광고를 위한 장바구니 분석 기법의 활용

김종우,이경미 한국경영과학회 2000 經營 科學 Vol.17 No.3

Customization and personalization services are considered as a critical success factor to be a successful internet store or web service provider. As a representative personalization technique, personalized recommendation techniques are studied and commercialized to suggest products or services to a customer of internet storefronts based on demographics of the customer or based on an analysis of the past purchasing behavior of the customer. The underlining theories of recommendation techniques are statistics, data mining, artificial intelligence, and/or rule-based matching. In the rule-based approach for personalized recommendation, marketing rules for personalization are usually collected from marketing experts and are used to inference with customers data. However, it is difficult to extract marketing rules from marketing experts, and also difficult to validate and to maintain the constructed knowledge base. In this paper, we propose a marketing rule extraction technique for personalized recommendation on internet storefronts using market basket analysis technique, a well-known data mining technique. Using marketing basket analysis technique, marketing rules for cross sales are extracted, and are used to provide personalized advertisement selection when a customer visits in an internet store. An experiment has been performed to evaluate the effectiveness of proposed approach comparing with preference scoring approach and random selection.

청가시덩굴(Smilax sieboldii Miq.)의 잎 및 지하부의 성분과 항고지혈작용에 관한 연구

조경열,우미희,오인숙 대구효성가톨릭대학교 응용과학연구소 1991 응용과학연구논문집 Vol.1 No.-

Nutritional value of the leaf and antihyperlipidemic effect of the subterranean part of Smilax siebolidd Miq.(Liliaceae) were investigated in this experiment. 8 minerals, 17 amino acids and 5 fatty acids were quantitatively analyzed in the leaf. In comparison with other vegetables, the content of amino acids was about twice as much as those and a lot of essential amino acids were found. The content of fatty acids was similar with that of other vegetables. In addition, various trace elements such as Mg, Zn and Mn were contained. The chloroform extract of the subterranean part of this plant was effective to decrease total cholesterol and triglyceride level and decrease HDL-cholesterol level in plasma of mouse.

간경변 환자에서 Oro-cecal Transit Time의 측정

원경준,이준,변유미,조민근,조주연,서정균,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background: Neurotransmitter like substances in the gut have been proposed to contribute to the overall portal systemic encephalopathy (PSE). The source of the ammonia in patients with PSE is mainly the small bowel, as a result of the conversion of glutamine in the diet to glutamate and ammonia. PSE is highly responsive to changes in diet, to antibiotic therapy and to ingestion of nondigestible disaccharides such as lactulose. This study was performed to evaluate the relationship between PSE and oro-cecal transit time. In addition, performed to evaluate the effect of severity, cause, complications of liver cirrhosis on oro-cecal transit time. Methods: Between August 2006 and February 2007, 48 cirrhotic patients consecutively was enrolled in study their oro-cecal transit time after informed consent was obtained. A non-invasive hydrogen breath test was used to study oro-cecal transit time (OCTT). Result: The OCTT were delayed in cirrhotic patients with PSE compared without PSE (123.3 ± 42.7 min vs 170.0 ± 58.3 min, p<0.05). OCTT tended to prolongate further as the damage in the liver got worse, nevertheless the result was statistically meaningless (p<0.111). Neither presence of ascites or cause of PSE had any influence on gastrointestinal transit. Conclusions: Cirrhotic patients with PSE have more delayed OCTT than without PSE. Further research is needed to find out the relationship between OCTT and severity of liver cirrhosis.

유자액을 이용한 소스의 이화학적 특성 및 저장성

유경미,서우영,서한석,김완수,박재복,황인경 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.4

The purposes of this study were to investigate the physicochemical characteristics and storage stabilities of various sauces with added Yuza(Citrus Junos). Yuza consisted of three parts: peeled (46.17%), fleshed (43.74%) and seeded (10.09%). Yuza juice revealed 11.33% extraction yield, pH 2.85, 4.18% total acidity, 11° Brix, 1.16% transmittance and 2.63° Brix/acid ratio. Five kinds of sauces with added Yuza juice were Yuza red pepper paste sauce, Yuza soybean sauce for meat, Shahu shabu Yuza sauce, Korean Yuza vegetable sauce and Japanese Yuza soybean sauce. These sauces showed slight changes in pH, total acidity, total bacterial counts and overall sensory characteristics during storage period (60 days). In conclusion, Yuza juice could be applied to various sauces, which remained usable for 60 days.