Lidocaine 이 흰쥐 해마박편에서 CA 1 추체세포의 활성에 미치는 영향

김형룡,민승기,김대업,오상천,유형근,김은철,고건일,원진숙 원광대학교 생명공학연구소 1995 생명공학연구소보 Vol.3 No.1

Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기 위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고,그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다. 1. B. abortus 분리 주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행한 바 공시한 참조균주와 분 리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다. 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리 균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B abortus B3196 (biovar 5)과는 99.6%∼100% 및 99.5%∼99.9%의 매우 높은 상동성을 나타내었고,B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상동성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바,분리균주들은 B.abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%∼100%의 매우 높은 상동성을 보였으며,B. abortus 45/20 (biovar 1)과는 96.4%∼ 97.2%의 상동성을 보였다. 3. 본 시험에 공사된 브루셈라균주의 omp2b 유전자 서열과 여러 동물에서 분리된 브루셀라 분리주의 자료를 바탕으로 phylogenetic tree를 작성 한 바,국내 분리주는 B. abortus 2308 (biovar 1),B. abortus B3196 (biovar 5),B. abortus 45/20 (biovar 1),B. suis 1330 (biovar 1),B. melitensis 16M (biovar 1),B. canis NCTC 01854,B. neotomae 5K33과 97.3% 이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며,omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OMP) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa aMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%∼100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고, 그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다 1. B. abortus 분리주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행 한 바 공시한 참조균주와 분리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.6%~l00% 및99.5%~99.9%의 매우 높은 상품성을 나타내었고, B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상품성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바, 분리균주들은 B. abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%~l00%의 매우 높은 상품성을 보였으며, B. abortus 45/20 (biovar 1)과는 96.4%~97.2%의 상품성을 보였다. 3. 본 시험에 공시된 브루셀라균주의 omp2b 유전자서열과 여러 동물에서 분리된 브루셀라 분리주의자료를 바탕으로 phylogenetic tree를 작성 한 바, 국내 분리주는 B. abortus 2308 (biovar 1), B. abortus B3196 (blovar 5), B. abortus 45/20 (biovar 1), B. suis 1330 (biovar 1), B. melitensis 16M (biovar 1), B. canis NCTC 01854, B. neotomae 5k33과 97.3%이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며, omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OW) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa OMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%-100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

포오트폴리오의 成果分析에 관한 硏究 : 證券投資信託 分析을 中心으로

金建浩,金炯郁,金喆中 홍익대학교 경영연구소 1984 경영연구 Vol.8 No.-

Ⅰ. Introduction The performance evaluation of portfolio should try to access whether the right assets were chosen at the right time and how well these assets were diversified. The purpose of this paper is to explain the issues about the performance evaluation of portfolio, and to analyze the Security Investment Trust in Korea. For this purpose, first I studied the capital market theory and the theory of portfolio performance evaluation model derived from the CAPM. Second, I selected 16 stock-typed in vestment trust funds and used several models. (Fama, Sharpe, Treynor and jensen's). Ⅱ. Models and sampling. Model 1 : Fama's Excess Return=γ-_p-γ-_f. Model 2 : Sharpe's Index(RVAR)=γ-_p-γ-_f/σ_p Model 3 : Treynor's Index (RVOL)=γ-_p-γ-_f/b_p Model 4 : Jensen's Measure(α) γ-_p-γ-_f=α_i+β_p(γ-_m-γ-_f)+e_i For the empirical test, 16 stock-typed investment trust funds are selected. Time span of each date (γ-_p,γ-_m,γ-_f) is one week from Jan. 1981 to Jun. 1984. Ⅲ. Results From the regression output, Ⅰ found the following results. ①Unsystematic risk of trust funds is more than systematic risk. But fund managers have been diversified better than common investors. ② The abnormal performance results calculated for all funds during the research period indicate that the mutual funds had performance on a gross return basis. ③ Our funds have no significant relations between their performance and betas. But these results may be corrected when we take account of average dividend ratio of market and transaction costs.

침 치료가 오십견(五十肩) 환자의 적외선 체열촬영에 미치는 영향

김건형,이로민,남동우,김종인,임사비나,이두익,최도영,이윤호,이재동 경희대학교 동서의학연구소 2006 東西醫學硏究所 論文集 Vol.2006 No.-

Objectives : To observe the effect of acupuncture treatment on Digital Infrared Thermographic Imaging(DITI) in frozen shoulder patients. Methods : 17 voluntary patients received acupuncture treatment on LI15, TE14, GB21 and Master Dong's acupuncture points, Shin-gwanand Gyun-joong, twice a week for 4 weeks. The patients were instructed to practice self exercise during their daily lives. Evaluations were made before treatment, after 1 week of treatment, after 2 weeks, 3 weeks and after 4 weeks of treatment. Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI), Range of Motion(ROM) and the patient's satisfaction concerning the treatment was measured by Visual Analogue Scale(VAS). DITI was measured before treatment and after 4weeks of treatment. The obtained data was analyzed. Results : CSA, SPADI, VAS, adduction and extension showed significant(p<0.05) improvement. Abduction and flexion both improved after 4 weeks of treatment, but the improvement was statistically insignificant(p>0.05). DITI showed improvement but the improvement was insignificant(p>0.05). Conclusion : 4 weeks of acupuncture treatment significantly improved CSA, SPADI, VAS, adduction and extension in frozen shoulder patients(p<0.05). The improvement of abduction and flexion after 4 weeks of acupuncture treatment was insignificant(p>0.05). DITI results improved after 4 weeks of acupuncture treatment. But the change of thermal difference was insignificant(p>0.05).

만성 견비통에 대한 동씨침 치료의 무작위 대조군의 임상 연구

김찬영,권나현,신예지,남동우,김건형,김종인,최도영,이윤호,이재동 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : To observe the effect of acupuncture treatment in chronic shoulder pain patients. Methods : 36 voluntary patients were randomly assigned to an acupuncture treatment group(E GrouP, n=18) and a control group(C GrouP, n=18). The E Group patients received acupuncture treatment on LI_(15), TE_(14), GB_(21) and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for four weeks. The C Group patients received no treatment. All patients in both groups were instructed to practise self exercise in their daily lives. Evaluations were made at baseline and after four weeks of study. The Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's subjective pain was measured by Visual Analogue Scale(VAS). The obtained data was analyzed. Results '. The E Group showed significant(p<0.05) improvement in CSA, SPADI and VAS after four weeks of treatment. The C Group showed significant(p<0.05) improvement in CSA, but the change of SPADI and VAS was insignificant(P>0.05). CSA and SPADI of E Group significantly(p<0.05) improved compared to the C GrouP, but the difference of VAS change in the two groups was insignificant(p>0.05). Conclusions : Four weeks of acupuncture treatment significantly improved CSA, SPADI and VAS. The improvement of CSA and SPADI was significant(p<0.05) compared to untreated patients.

SGCI 材料의 Casting 및 磨滅强度에 關한 硏究

김성주,문형태,최익수,노무근 三陟大學校 産業科學技術硏究所 2003 産業科學技術硏究論文集 Vol.8 No.2

In order to collect the basic data for the production of nodular cast iron requiring wear resistance. the mechanical nature such as structure analysis, hardness, and tensile strength were performed on cast sample by changing residual Mg concentration of cast iron at the range of 0∼0.052% and also the wear characteristics were examined through wear test by changing abrasion speed and final load. As a result, the following conclusions were drawn. The wear amount rapidly accelerates to the maximum abrasion sped of 2.3m/sec in a sample without Fe-Si-Mg alloy processing(residual Mg amount; 0%) by increasing the abrasion speed from 1.3m/sec to 2.3m/sec. As the Mg residual Mg contents increases, the ratio of nodular increases while hardness of a sample accelerates and the wear amount decreases. The maximum point moved to the high speed side in a sample with 0.043% residual Mg contents and the wear amount showed the maximum in 3.5m/sec of abrasion speed while a sample with 0.052% residual Mg contents showed the mechanical destruction wear to 4.3m/sec abrasion speed. The more residual Mg contents are in 0.6-l.1m/sec low speed range of abrasion speed, the more wear amount was resulted while the less residual Mg contents were. the less wear amount showed. Oxidation wear occurs at 4.5m/sec of traveling velocity. Since the wear of matrix structure occurs after wear of oxidized substance in friction caused by oxidized substance, the wear amount shows less than 2.3mg/sec traveling velocity. Therefore, as the residual Mg contents increase, the wear amount decreases. Although the wear amount is large since the traveling distance of destruction wear lasts very long. the residual Mg contents are small and the oxidation wear area becomes long in a sample with low hardness. Since the mechanical destruction wear occurs when the traveling &stances reaches to the end, the wear amount results in very small. The scratches appeared due to abrasive wear in mechanical destruction wear with 2.3/sec abrasion speed while it did not show in oxidation wear although the severe wear occurred. The sectional melting appeared in 5.4m/sec of high speed friction since the temperature of contact point increased more than 1000℃. Scratches are less in the area with low load and if load increase, shoving occurs in wear grooves. In the maximum load, the severe cracking phenomenon can be observed.

CuInSe₂의 결정구조에 관한 연구

김형관,이건상,김옥경 漢陽大學校 基礎科學硏究所 1988 基礎科學論文集 Vol.7 No.-

Se를 화학당량비보다 10% 더 첨가하여 2단전기로를 사용하여 용융법에 의해 성장시킨 CuInSe₂의 결정화 여부를 x-ray diffractometer로 측정한 회절무늬와 계산된 회절무늬를 비교하여 결정하였다. 성장된 결정은 격자상수가 a=5.78Å과 c=11.59Å인 chalcopyrite 구조를 가지며 c/a=2.005로 신장 외곡을 하고 있다. Ⅵ족 원자의 위치를 결정하는 값 x는 x=0.2487이며, ??=2.5007Å, ??=2.5095Å으로 Se가 Cu쪽에 가깝게 위치하고 있다. 따라서 성장된 결정은 이온분극을 가진 것으로 해석된다. CuInSe₂ crystals are grown by directional freezing method using two-zone furnace. The crystal structure are investigated using x-ray diffractometer by powder method and compared with calculated data. The lattice constants are calculated a=5.78Å and c=11.59Å. The distance between atoms are ??=2.5007Å and ??=2.5095Å. It is found that Se atoms are positioned toward Cu atoms.

球狀黑鉛鑄鐵의 熔解 및 製造工程에 關한 硏究

盧戊根,金大壽,文亨泰 三陟大學校 2000 論文集 Vol.33 No.2

The purpose of this study is to establish the fundamental informations of melting and manufacturing processes of spheroidal graphite cast iron by means of experiments. The main results obtained are as follows. The tensile strength of 45kg/㎟ SGCI was produced at the residual Magnesium of 0.04%, and the spheroidization treatment temperature of 1500℃. thickness of refractories 45cm above is the appropriate condition of manufacturing the furnace, and the sintering experiment Ⅱ process gives the stability of production processes. The higher the temperature of casting sand, the more specimen weight and moisture contents The higher the moisture contents of casting sand, the lower the compressive strength of mold.