영양표시정착화사업(Ⅱ) : 식품참고량설정에 관한 연구 Study on the Establishement of the Reference Amount in Foods

김대병,장영미,김인복,이혜영,장재희,김보영,이정구,김창민,정해랑 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

본 연구는 영양표시에서 1회분량 및 영양소함량표시의 기준으로 사용되는 "식픔창고량"에 관한 연구를 수행하므로써 합리적이고 일관성있는 영양표시기준 개선을 위한 기초자료로 활용코자 하였다. 이를 위하여 미국 및 캐나다를 중심으로 제외국의 현황 및 식품참고량 설정방법을 조사 ·연구 하으며 제도도입 및 개선 방안을 강구하였파. 또한, 시판 제품의 영양료시현황 및 음료류 둥의 포장단위 등을 피악하여 식품참고량설정을 위한 기초자료로 활용코자 하였다. 앞으로 국민건강 ·영양조사자료의 체계적인 통계분석, 가공식품의 섭취량조사 등을 통하여 우리나라 국민의 1인 1회 섭취량자료의 축적이 선행되어야 할 것이다. The purpose of this ?roject is to study on the reference amounts customarily consumed per eating occasion to improve the nutrition labelling standards. We Performed to study on the methodology to establish the reference amounts on the basis of the review on the advanced counITy's status. And we investigated the packaged unit ofprocessed foods. Further study is needed to establlsh the customarily consumed amount per eating occasionthrough the statistical analysis of nationwide food consumption data. Our results will be helpful information to improve nutrition labelling system in Korea.

건강식품 바이탈 및 비지놀의 抗疲勞 效果에 관한 연구

정경수,김혜경,김희수,김계양,곽영숙,김학범 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

As a result of extensive research efforts on the oriental medicinal herbs, some of their interesting biological activities have been elucidated. In the present study, antifatigue effects of two natural food supplements consisting of oriental herbs, Vital and Vigilor, were investigated using the ICR mice. The mice were fed with antibiotic-free normal mouse feed containing Vital or Vigilor for 14,28, or 31 days and then subjected to forced swim tests. In a test carried out in melting-ice water, Vital and Vigilor significantly increased the exhaustion times of the 28 day-fed mice by 26.9 % (p < 0.01) and 16.2% (p < 0.05), respectively. However, in the other test performed at 15±0.5℃ using 31 day-fed mice, Vigilor failed to increase the exhaustion time significantly (at p< 0.05), while Vital showed antifatigue effect by increasing the swimming time by 29.9% (p<0.01). Neither of Vital nor Vigilor exerted antifatigue effects in a swim test carried out on day 14 after the initiation of feeding. These results suggest that Vital and Vigilor exhert antifatigue effets when administered for a sufficient period of time by enhancing the health of the host animals.

중년여성 레크리에이션 프로그램 효과 검증

김정현(Jung Hyun Kim),최혜련(Hye Lyeon Choi),이충영(Chung Young Lee) 한국사회체육학회 2011 한국사회체육학회지 Vol.0 No.43

This study aims to design recreation programs and provide them for middle aged women in order to decrease the levels of depression and stress, and improve the level of self-esteem that they experience. Special recreation programs serving for these purposes were invented by combining motions, dances, games and songs, and then applied to 374 middle aged women who resided in Kyung ki-Do and Chung chung-Do. A 5-point Likert type scale was used for the questions, and the same survey was carried out before and after the respondents participated in the recreation programs, in order to find whether the applied recreation programs were effective. The results of the quantitative analyses support the positive role of well-designed recreation programs: First, the recreation programs significantly decreased the level of depression of middle aged women; Second, they significantly reduced the level of stress of middle aged women, and; Finally they significantly improved the level of self-esteem of middle aged women.

여고생의 교복 및 장식 행동에 관한 연구 : 경상남도 마산·창원 지역을 중심으로

정혜경,김세희 服飾文化學會 2004 服飾文化硏究 Vol.12 No.1

The purpose of this study was to examine how high school girl students used their school uniforms and what types of ornaments they preferred, in an effort to identify the image of school uniform. The result of this study were as follow: First, 52.6 percent of the students investigated altered their uniforms, and as for the major alteration style, revival one to take them in to be well-fitting was dominant. But concerning skirts, hip-hop style was also found. The students viewed ornaments more favorably, as the larger group of the students perceived that it was necessary to restrict the school-uniform alteration yet not to control ornaments. Concerning how much they wore school uniforms, most of them continued to wear them outside school in many cases. Specifically, those who engaged in pastime or went shopping wearing school uniforms enjoyed altering them, and they also enjoyed using ornaments that weren't allowed by the school dress code. Second, as to school-uniform image, elegant image was most preferred, followed by practical, innocent and fashion-conscious ones. They were most preferred with its elegant image. Third, as to ornaments image, they were most preferred with its positive image.

Streptococcus mutans의 gtfB 및 gtfC 유전자 발현에 대한 당의 영향

정혜진,김신,정진 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2

Streptococcus mutans Ingbritt의 비수용성 글루칸을 합성하는 효소인 glucosyltransferase B 및 C의 mRNA 발현에 대한 각 당의 영향을 fluorescent in situ hybridization (FISH) 방법으로 관찰하여 다음의 결과를 얻었다. 1. 1% 설탕이 함유된 BHI 배지에서 S. mutans Ingbritt를 접종하여 배양할 때 배양 9시간 째 gtfB 및 gtfC 유전자의 mRNA가 발현이 급격히 증가하였다. 2. BHI 액체배지에 설탕을 첨가한 경우 gtfB 및 gtfC 유전자의 mRNA가 발현되었으며, 10% 설탕을 첨가한 경우 1%와 5%보다 gtfB 및 gtfC 유전자의 발현이 감소되었다. 3. 1% 설탕이 첨가된 BHI 액체 배지에 당을 첨가한 경우 포도당은 10% 첨가하였을 때 gtfB 및 gtfC 유전자의 mRNA 발현이 감소하였고, 과당의 경우 1% 첨가한 경우 감소하여 5%와 10%에서는 gtfB 및 gtfC 유전자가 거의 발현되지 않았다. 4. 자일리톨의 경우 1%부터 gtfB 및 gtfC 유전자 mRNA 발현이 대조군보다 감소하였고, 5%와 10%에서는 두 유전자 모두 발현이 현저하게 감소하였다. 5. 유당의 경우 두 유전자의 발현에 거의 영향을 미치지 않는 것으로 관찰되었고, 솔비톨의 경우 각 농도에서 두 유전자의 발현이 대조군보다 감소하였다. 결론적으로 비수용성 글루칸 합성하는 gtfB 및 gtfC 유전자의 mRNA 발현이 포도당, 과당, 자일리톨 첨가에 의해 감소함을 알 수 있었다. Insoluble glucan is the important component of oral biofilm, which is synthesized from sucrose through the action of glucosyltransferase (GTF) B and GTF C encoded by the gtfB and gtfC genes, respectively of Streptococcus mutans. In present study, the effects of various sugars on the mRNA expression of gtfB and gtfC of S. mutans Ingbritt were examined by fluorescent in situ hybridization (FISH). The mRNA of gtfB and gtfC was expressed normally in the BHI broth containing 1% and 5% sucrose. The mRNA expression was decreased by the addition of 10% of glucose, and 1%, 5% and 10% of fructose. Lactose had no great effect on the expression of gtfB and gtfC 5% and 10% of xylitol greatly reduced the mRNA expression of gtfB and gtfC. Sorbitol slightly decreased the mRNA expression of gtfB and gtfC when compared to the control. In summary, mRNA expression of gtfB and gtfC was decreased by the addition of glucose, fructose, and xylitol.

자궁경부 상피내종양의 진단 및 치료에서 환상 투열요법(LLETZ)의 임상적 의의

김승철,이경순,김영주,정혜원,안정자 梨花女子大學校 醫科大學 醫科學硏究所 1994 EMJ (Ewha medical journal) Vol.17 No.4

The technique of large loop excision of the transformation zone(LLETZ) as an alternative to destructive/ablative methods of treatment of CIN(cervical intraepihelial neoplasia) such as cryotherapy, laser vaporization and cone biopsy was assessed. In 63 women with suspected CIN attending the colposcopy clinic, the histologic diagnoses made from excision biopsies taken by LLETZ were compaired with those from colposcopically directed punch biopsies. This microinvasive tumors were found by LLETZ and the diagnosis made by LLETZ was worse than that made by punch biopsy in 17 cases(26.9%). That is to say, LLETZ improves the accuracy of diagnosis of CIN. And in offers a cheap and accurate alternative to laser vaporization or cone biopsy, and also combines the benifits of the gold standard cone biopsy with that of local ablative metholds. LLETZ is considered as a fairly new and good method of treatment of CIN, and the olng-term complications or effects should by yet fully evaluated in future.

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

아끼바레 및 밀양 23호 쌀전분의 이화학적 성질비교

정혜민,안승요,김성곤 한국응용생명화학회 1982 Applied Biological Chemistry (Appl Biol Chem) Vol.25 No.2

아끼바레 및 밀양 23호 쌀 전분의 이화학적 성질, 전분 gel의 노화 및 음이온에 의한 호화양상을 조사하였다. 두 전분입자의 성상은 다각형이며 입자의 크기는 3~6㎛정도였으며, 아끼바레가 밀양 23호 보다 다소 작았다. 두 전분의 X-ray 회절도는 A형으로 peak 형태와 강도에서 약간의 차이를 보였다. 아끼바레 및 밀양 23호 쌀 전분의 아밀로스 함량은 각각 18.5 및 19.5%이었고 물 결합능력은 106 및 100%이었다. 팽화력은 50℃까지는 큰 변화가 없었으나 이후에 급격히 증가하였고 밀양 23호 쌀 전분의 팽화력이 더 컸다. 전분 현탁액(0.1%)의 광투과도는 두 전분 모두 60℃ 이후에서 급격히 증가하였으며 밀양 23호 쌀 전분이 더 큰 광투과도를 보였다. 음이온에 의한 아밀로그람 pattern은 NaCl과 NaF는 동일하였고, NaSCN과 NaI는 유사였다. 호화온도 및 최고점도시의 온도는 SCN^(-)<I^(-)<Cl^(-)<F^(-)<SO₄^(=)의 순으로 증가하였고 SCN^(-) 및 I^(-)이온은 최고 점도를 증가시켰으나 NaCl은 감소시켰다. SO₄^(=)이온을 첨가한 경우 점도는 계속 증가하였고 최고점도에 도달하지 않았다. 아끼바레 및 밀양 23호 전분 gel율 21℃에서 저장한 경우 시간 상수가 각각 6.72 및 6.42일이었다. Morphology, pbysicochemical properties, pasting properties in the presence of various anionic ions and aging of gels of Akibare (Japoica type) and Milyang 39 (Indica type)rice starch were studied Both starches. were polygonal with length in the range of 3~6㎛. Starch granules of Akibare were somewhat smaller than those of Milyang 23. X-ray diffraction study demonstrated that peak shape and intensity were significantly different between the two starches. Akibare and Milyang 23 rice starch had amylose content of 18.5 and 19,5% and water binding capacity of 106 and 100%, respectively. Milyang 23 rice starch had a higher swelling power than Akibare starch. A relationship between percent solubility and swelling power implied that bonding forces within the granules of the both starches were different. The optical transmittance of 0.1% suspension of the two starches increased rapidly from 60℃. In the range of 60~90℃, the two starches showed a single gelatinization pattern. Amylograms of the two starches in the presence of various anionic ions showed that pasting temperature and peak temperature were progressively increased in the order of SCN-<I-<CI-<F-<SO₄=. SCN- and I- ions increased the peak height of Akibare rice starch white only SCN- ion was effective for Milyang 23 rice starch. There were no differences in the rates of retrogradation of 45% gels of the two starches stored at 21℃.

WBI에 기반을 둔 교수-학습용 웹 코스웨어의 설계 및 구현

정재열,김혜경 신라대학교 교육과학연구소 1999 교육과학연구 Vol.- No.4

페놀을 사용한 신경 차단술이 경직성 뇌성마비아동의 경직과 보행에 미치는 영향에 관한 정량적 연구

김봉옥,조강희,정혜심 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.2

The effectiveness of a peripheral nerve block with 7% phenol solution in children with spastic cerebral palsy was evaluated in 37children quantitatively. Kinematic and kinetic data as well as the clinical data were obtained in the computerized motion analysis laboratory before and after the nerve block. Dynamic electromyographic data was also collected from the muscles of the legs with the multichannel equipment before and after the blocking procedure. Obturator and tibial nerves were most frequently blocked. All the measurement data obtained showed significant improvement of function after the nerve block with phenol until 3 months after the block. After 6 months. satisfactory outcome was maintained in 42.9% of the children. The peripheral nerve block with 7% phenol solution is a effective and affordable method in the management of spasticity in the children with spastic paralysis in the carefully selected cases.