DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

근린공원에 대한 환경지각이 이용자의 건강증진인식에 미치는 영향 - 창원시의 8개 근린공원을 대상으로 -

박영은,이우성,정성관,박경훈,Park, Young-Eun,Lee, Woo-Sung,Jung, Sung-Gwan,Park, Kyung-Hun 한국조경학회 2015 韓國造景學會誌 Vol.43 No.1

The purpose of this study was to analyze the effect of environmental perception in neighborhood parks on user's recognition of health improvement. This study used objective field survey data and subjective user survey data at 8 neighborhood parks in Changwon City. According to the results, the perceptions of distance and street environment from home to park were evaluated highly but the perception of water spaces and various attractions within the parks were rated lowly in most of parks. As a result of factor analysis, 23 environmental perception variables were classified into 6 factors such as scenery, comfort, accessibility, activity, convenience and amenity. For the result of regression analysis between environmental perceptions and user's recognition of health improvement based on 6 factors, the recognition of physical health improvement was significantly influenced from environmental perception factors in 4 parks among 8 parks. 'Accessibility' and 'activity' were analyzed as the meaningful factors in 3 parks. Also, recognition of mental health improvement was significantly influenced from environmental perception factors in 5 parks among 8 parks and especially 'accessibility' was showed as the significant factor in 4 parks. The findings from this study can contribute to improve the physical environments of the present parks and can be a basic data for new park development.

군사시설 이전부지 공원화 사례분석을 통한 시사점 도출

박준영,이은엽,송선영,엽정식,Park, Joon-Young,Lee, Eun-Yeob,Song, Sun-Young,Yeob, Jung-Sik 한국토지주택공사 토지주택연구원 2014 토지주택연구 Vol.5 No.4

Because of their nature as public facilities, demands that former military facilities be utilized for the public welfare are increasing, thereby leading to an increase in cases in which these military facilities are reestablished as parks. Cases in which former military bases were reestablished as public parks were analyzed; as a result, several implications were derived. First, the objectives of public park projects should be examined from the perspective of the concept of urban regeneration and regional revitalization. Moreover, it is necessary that profits are yielded and that regional identity and history are reproduced through reusing existing facilities as much as possible rather than entirely remodeling former military sites. As parks become larger in size, bases should be reorganized into complexes rather than single facilities or programs. It is also necessary that parks be established in stages considering the enormous expenses required for building public parks. Consequently, because the special characteristics of military facilities can lead to insufficient on-site investigation in the process of establishing parks, thereby incurring a vast amount of costs for design adjustment and contamination disposal, this should be considered in advance. A method of delegating the development rights to partial sites to private businesses and supplementing the costs of park establishment and maintenance with development benefits should be examined. In addition, given that there are various interests and stakes in former military bases, a method of operating a public-private cooperative decision-making organization during project execution should be considered. Finally, policies related to urban parks need to be improved in order to raise funds, expand profitable businesses, facilitate social services and sponsorship, and encourage the participation of trusts and non-profit organizations in park operation and management.

근린공원 환경의 만족도가 신체활동과 건강에 미치는 영향 - 창원시 성산구와 의창구를 대상으로 -

박경훈,이우성,김태환,김은정,Park, Kyung-Hun,Lee, Woo-Sung,Kim, Tae-Hwan,Kim, Eun-Jung 한국조경학회 2014 韓國造景學會誌 Vol.42 No.3

The purpose of this study is to analyze the effects of satisfaction in neighborhood park environments located in the Changwon-si of Gyeongsangnam-do, South Korea, on physical activity, the number of parks used for health improvement, and health levels based on Body Mass Index(BMI). Accordingly, a survey was conducted of 429 nearby residents and users of eight neighborhood parks located in urban areas of Seongsan-gu and Uichang-gu in the Changwon-si. The correlation between the environmental perception of neighborhood parks and physical activity and health, which was observed in the survey results, was analyzed using one-by-one linear regression analysis. By summarizing the study results, it was found that the primary reasons for avoiding park use were lack of time, time and effort required to reach the park(i.e., distance from the park), and lack of facilities within the park. Conversely, the primary reasons for using the park included accessibility, walking or strolling, leisure facilities, trees or shade, and diversity in exercise areas. In the case of park users, walking or strolling was the most common activity in the park. On average, park users walked to the parks for 10 minutes and exercised for an hour at least twice per week. With respect to the physical environment of the parks and surrounding areas, park users showed a high level of satisfaction with the distance between their houses and parks and the street environment. On the contrary, they exhibited low levels of satisfaction with water spaces and sightseeing within the parks. Subsequently, it was shown that the number of people using the parks for physical activities and health improvement was positively influenced(within a 5% significance level) by the intent to use the park for exercise, time spent in the park, satisfaction with park use and health improvement, distance to the park, and the convenience of using the park for the elderly. However, only the health improvement gained from park use was found to exhibit a correlation with BMI at the 10% significance level. Continuous accumulation of practical case studies on physical activities in parks and their health improvement effects is required. Through this, park spaces that are under the threat of reduction or elimination owing to various development plans can be conserved and expanded. Furthermore, such case studies can be used to provide data as the basis for deriving park plans and designs that improve parks' functions as sites of physical activity and health improvement.

거주지 주변 환경에 대한 인식이 건강증진 목적의 공원이용에 미치는 영향 - 대구광역시 수성구를 대상으로 -

이우성,정성관,박영은,Lee, Woo-Sung,Jung, Sung-Gwan,Park, Young-Eun 한국조경학회 2015 韓國造景學會誌 Vol.43 No.6

The purpose of this study is to analyze the effect of neighborhood environmental perception on park use for health. The study area is focused on neighborhood parks in Suseong-gu, Daegu city. The analysis results are based on questionnaires of 303 respondents. According to the results, park use for health improvement was significantly associated with the perceptions of accessibility to a marketplace, availability of public transit, accessibility to the park, enough sidewalks, number of crosswalks, and athletic population. The probability of park use increased by 2.326 times as the satisfaction of accessibility to the park increased. Also, greater athletic population, fewer crosswalks, and more sidewalks lead to increase of park use by 1.701, 1.617, and 1.642 times, respectively. Therefore, it is important to increase the available neighborhood parks around residences and to develop exercise programs that people can use continuously in order to vitalize park use for health improvement. Also, it is necessary to improve the convenience of walking such as development of pedestrian streets or malls between residences and parks.

Insulin resistance mediates high-fat diet-induced pulmonary fibrosis and airway hyperresponsiveness through the TGF-β1 pathway

Park, Yoon Hee,Oh, Eun Yi,Han, Heejae,Yang, Misuk,Park, Hye Jung,Park, Kyung Hee,Lee, Jae-Hyun,Park, Jung-Won Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.5

<▼1><P>Prior studies have reported the presence of lung fibrosis and enhanced airway hyperresponsiveness (AHR) in mice with high-fat-diet (HFD)-induced obesity. This study evaluated the role of TGF-β1 in HFD-induced AHR and lung fibrosis in a murine model. We generated HFD-induced obesity mice and performed glucose and insulin tolerance tests. HFD mice with or without ovalbumin sensitization and challenge were also treated with an anti-TGF-β1 neutralizing antibody. AHR to methacholine, inflammatory cells in the bronchoalveolar lavage fluid (BALF), and histological features were evaluated. Insulin was intranasally administered to normal diet (ND) mice, and in vitro insulin stimulation of BEAS-2b cells was performed. HFD-induced obesity mice had increased insulin resistance, enhanced AHR, peribronchial and perivascular fibrosis, and increased numbers of macrophages in the BALF. However, they did not have meaningful eosinophilic or neutrophilic inflammation in the lungs compared with ND mice. The HFD enhanced TGF-β1 expression in the bronchial epithelium, but we found no differences in the expression of interleukin (IL)−4 or IL-5 in lung homogenates. Administration of the anti-TGF-β1 antibody attenuated HFD-induced AHR and lung fibrosis. It also attenuated goblet cell hyperplasia, but did not affect the AHR and inflammatory cell infiltration induced by OVA challenge. The intranasal administration of insulin enhanced TGF-β1 expression in the bronchial epithelium and lung fibrosis. Stimulating BEAS-2b cells with insulin also increased TGF-β1 production by 24 h. We concluded that HFD-induced obesity-associated insulin resistance enhances TGF-β1 expression in the bronchial epithelium, which may play an important role in the development of lung fibrosis and AHR in obesity.</P></▼1><▼2><P><B>Obesity: A trigger for asthma onset</B></P><P>Insulin resistance may be an important causative factor underlying the increased risk of asthma and other respiratory issues in obese individuals. Obesity doubles the likelihood of developing asthma, with symptoms that are more difficult to control than in non-obese patients. The connection between these conditions is poorly understood, but researchers led by Jung-Won Park, Yonsei University College of Medicine, Seoul, South Korea, have identified a potential mechanism. They demonstrated that a signaling molecule called TGF-β1 contributes to airway sensitivity and tissue scarring in a mouse model of diet-induced obesity. Subsequent experiments showed that treatment with insulin also gives rise to increased TGF-β1 production in the mouse lung. Since insulin resistance is a common feature of obesity, resulting in abnormally high levels of circulating insulin, this could also account for the increased risk of respiratory problems.</P></▼2>

GIS 네트워크 분석을 활용한 중구 화재 취약 지역 분석

장경은(Kyung-Eun Jang),정유미(Uemee Jung),박초롱(Chorong Park),이종호(Jongho Lee),손동욱(Dongwook Sohn) 한국생태환경건축학회 2021 한국생태환경건축학회 논문집 Vol.21 No.6

Purpose: A fire in the city, classified as a social disaster, does huge damage to life and property. In Particular, the threat is being increased by the concentrated population density, the increase in the number of buildings and complex land use according to the deepening urbanization.Therefore, it is important to prevent a fire in advance by observing the golden time for fire suppression, rescue and first-aid activities. However, the issue of illegal parking is continues to be discussed as one of the factors that hinder the dispatch of fire trucks, and the increase in private vehicles in Seoul is expected to further increase the difficulty of accessing fire trucks in the future. Therefore, the purpose of this study is to grade vulnerable areas for fire through comprehensive judgment on the arrangement of emergency medical facilities and 119 safety centers and illegal parking. Method: Using fire outbreak data and Arc GIS Pro for Jung-gu, Seoul, comparison of fire fighting jurisdictions of the 119 Safety Center and areas that can be dispatched within golden time, emergency medical treatment After analyzing the correlation between the possible emergency transport distance to the facility within 5 minutes, public parking lot and illegal parking CCTV, and the occurrence of fire, the fire safety area and vulnerable area were derived through density analysis. Result: In the event of a fire in each administrative dong in Jung-gu, areas that are marginalized from initial suppression and evacuation measures were derived, and realistic and reasonable complementary measures according to the regionalization of administrative districts were suggested by proposing the relocation and expansion of fire fighting facilities and emergency medical facilities, and regional adjustment.

서울·경기지역 병원직원의 혈액매개 감염노출에 대한 다기관 조사연구

박미라,김정은,박은숙,최정실,정선영,송영구,홍성관,김준명,허애정,양동규,조영주 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.1

중환자실 병원감염에 대한 다기관 공동 조사연구

박은숙,박미라,진혜영,김정은,최정실,송영구,최영화,이위교,홍성관,허애정,양동규,김준명 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.1

수입각증후군에 의한 급성 복증 1례

정은욱,지삼룡,이영태,박지훈,김동기,제인수,채두근,박성재,박은택,이연재,이상혁,설상영,정정명 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Afferent loop syndrome is an uncommon complication of a gastrectomy and Billroth Ⅱ reconstruction. It may cause symtoms at any time from the first postoperative day to many years after the gastrectomy. Afferent loop syndrome is characterized by abdominal pain, vomiting and elevation of serum amylase. Thus, it is difficult to differentiate afferent loop syndrome from other cause of acute pancreatitis. However, the history of gastrectomy can be an important clue for diagnosing afferent loop syndrome. We experienced one case of chronic afferent loop syndrome with acute pancreatitis. After appropriate management, the abdominal pain disappeared and serum amylase level decreased. We report this case with a review of relevant literatures.