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RISS 인기검색어
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Real - Q RV Detection Kit를 이용한 호흡기 감염을 유발하는 바이러스의 동정 성능 평가
이은엽,신새암,김미영,이영경,강희정,김현수,김재석,송원근,김한성 대한진단검사의학회 2019 Laboratory Medicine Online Vol.9 No.1
Viral respiratory infections are one of the most common infections worldwide. It is important to detect the virus early and precisely. In this study, we evaluated the limit of detection (LoD) and usefulness of the Real-Q RV Detection kit (BioSewoom, Seoul, Korea). We measured the LoD of the Real-Q RV Detection kit using 10 strains of standard viruses. We then compared the detection results by the Allplex Respiratory Panel Assay kit (Seegene, Seoul, Korea) using 123 clinical specimens. The discrepant results were confirmed by sequencing. Among the 10 standard viruses, the LoD of human rhinovirus (HRV) was the lowest and that of parainfluenza virus 2 and 3 was relatively high as detected by Real-Q RV Detection kit. Agreements of the two kits ranged from 95.9% to 100%. Three specimens detected negative by the Allplex Respiratory Panel kit were detected as adenovirus (AdV) by the Real-Q RV Detection kit and were confirmed by sequencing. Similarly, a specimen detected negative by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. A specimen detected as human enterovirus by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. Real-Q RV Detection kit showed good diagnostic performance and can be useful for detecting major viruses that cause respiratory infections. 호흡기바이러스 감염은 세계적으로 가장 흔한 감염성 질환이며 사망을 유발하는 주요 질환 중 하나로 신속하고 정확한 원인바이러스의 검출이 중요하다. 이번 연구에서 실시간 다중 PCR kit 인 Real-Q RV Detection kit(BioSewoom, Korea) 의 호흡기 바이러스 검출한계와 임상적 유용성을 평가하고자 하였다. 총 10 주의 표준 바이러스를 대상으로 하여 Real-Q RV Detection kit 의 검출 한계를 측정하였으며, Allplex Respiratory Panel Assay kit (Seegene, Korea) 와의 비교를 위해 123 개의 비인후 도찰물, 객담 등의 호흡기 검체를 대상으로 하여 제조사의 권고방법대로 검사하였다. 두 kit 간 불일치 결과를 보이는 검체는 염기서열 분석을 시행하였다. 표준바이러스 중 Human rhinovirus (HRV) 의 검출한계가 가장 낮았으며, Parainfuenza virus 2 (PIV 2) 와 Parainfuenza virus 3 (PIV 3)는 상대적으로 높은 검출한계를 보였다. 두 kit 간 일치율은 95.9%에서 100% 까지 나타났다. Real-Q RV Detection kit 에서 Adenovirus (AdV), Allplex Respiratory Panel kit 에서 음성을 보인 검체(3건)는 염기서열분석 결과 모두 AdV 가 확인되었고, Real-Q RV Detection kit 에서 HRV, Allplex Respiratory Panel kit 에서 음성을 보인 검체(1 건)는 염기서열분석 결과 HRV 가 확인되었다. Real-Q RV Detection kit 에서 HRV 로 양성 , Allplex Respiratory Panel kit 에서 Human enterovirus (HEV)가 검출된 검체(1건)는 염기서열분석 결과 HRV 가 확인되었다. Real-Q RV Detection kit 는 호흡기 바이러스의 검출에 있어서 유용할 것이다.
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간세포암 환자의 혈청 Des-γ-Carboxy Prothrombin, Alpha-Fetoprotein 및 Anti-p53 항체검사의 유용성
이은엽,박태희,김순호,문태용,황은주,조 몽 대한소화기학회 2001 대한소화기학회지 Vol.37 No.6
Background/Aims: Des-γ-carboxy prothrombin (DCP) and α-FP have been used for diagnosing hepatocellular carcinoma (HCC). Recently, anti-p53 antibodies have been detected in sera of the patients with cancer. The diagnostic significance of the combination assay of DCP, α-FP, and anti-p53 antibodies was evaluated in patients with HCC. Methods: For 78 cases of HCC, 17 cases of liver cirrhosis, 32 cases of chronic hepatitis, and 23 healthy controls, the serum levels of DCP, α-FP and anti-p53 antibodies were measured by enzyme immunoassay. Their sensitivity, specificity and accuracy were also assessed. Results: In patients with HCC, the sensitivity of DCP and α-FP was 52.9% and 66.7%, which were significantly higher than 7.7% of anti-p53 antibodies. In detecting HCC, the specificity of DCP, α-FP and anti-p53 antibodies was 91.8%, 89.8% and 98.0%, and their accuracy was 69.2%, 75.8% and 45.2%, respectively. No correlation was found among three tumor markers. The sensitivity and accuracy in the combination assayP, α-FP and anti-p53, and in that of DCP and α-FP were higher than in assay of each tumor marker or other combination, especially in small sized HCC. Conclusions: A combination assay of DCP, α-FP, and anti-p53 antibodies and a combination assay of DCP and α-FP are useful for diagnosis or early detection of HCC, but the diagnostic usefulness of anti-p53 antibodies is still uncertain because of its low sensitivity.
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이은엽,신병철,조태동 한국환경과학회 2004 한국환경과학회지 Vol.13 No.12
The purpose of this study is to select the appropriate wild herbaceous plants on the rooftop. For the purpose of the experiments, 14 wild herbaceous plants were chosen. As the results of the experiments, the wild herbaceous plants growing on the culture soil(perlite) Among the 14 kinds of wild herbaceous plants, such as Chrysanthemum boreale, Taraxacum mongolicum, Aster Koraiensis, Aster yomena, Oenothera odorata, Oenothera lamarckiana, Patrinia scabiosaefolia showed good effects on growth of above ground parts. Therefore, these plants will effective for the rooftop revegetation. Callistephus chinensis and Lotus corniculatus var. Japonicus showed good height growth, wherase covering rate was worst. Chrysanthemum boreale, Platycodon grandiflorum, Patrinia scabiosaefolia were plant height, wherase covering rate was not good . For the expected number of seedings more than 3,000 per square meter, many seedings got withered to death while the survivors were suppressed to grow slow in the early stage due to the densityproblem.
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이은엽,문석기,심상렬 한국조경학회 1996 韓國造景學會誌 Vol.24 No.1
To study the temperature mitigation effects from urban green, several arrangements of green spaces were selected and air/ground temperatures were measured in Chongju city area. The results of this study can be summarized as follows; 1. It was found that the natural ground materials effect more affirmatively on the air and ground temperature than artificial ones do. The best results were recorded from the grass surface presenting highest mitigation effect and lowest daily temperature deviation. 2. Temperature mitigation effects of Tree-Shade on ground are different from season, ground material, and crown-size. Them most effects were found in interlocking block, the least in grass surface among recorded 2 seasons and 3 materials. In case of air temperature, the effects were more or less decreased in most cases. 3. From the survey, it was confirmed that the smaller urban greens can do its role of temperature mitigation as larger ones does. In case of this study, the effect was recorded about 2.3$^{\circ}C$.
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