八公山 自然公園의 利用者 滿足에 關한 因果模型 硏究 : Case Study of Palgong Natural Park in Taegu

朴贊龍,玄重英 영남대학교 자원문제연구소 1993 資源問題硏究 Vol.12 No.-

This research suggests a causal model to investigate factors and variables which significant effects on user satisfaction with natural park recreational facilities in Palgong Natural Park in Daegu, and thereby identifing implications for planning and development of urban natural parks and provincial parks. For this study data were gathered by self- administered question-nares from 933 households selected by the multi - stage probability sampling method. The analysis of the data consists of two phases. The first involves exploratory factor analysis of the draw meaningful factors from the data. Four factors were identified. The second phase test the casual model of this research employing LISREL methodology. On the base of the analysis results. important implication for planning urban natural park and provincial park are recommended.

이면성 및 도플러 심초음파도를 이용하여 진단한 선천성 거대 좌회선지-좌심실 관동맥루

진승원,박준철,이영중,유병현,박현옥,김용주,윤호중,김재형,홍순조,최규보,권종범,원용순,박건 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

주파수 응답 함수를 이용한 평면 밀링에서의 채터 예측

홍성욱,박중윤,김현수,박재현,박창용 金烏工科大學校 産業技術開發硏究院 1999 産業技術開發硏究 Vol.15 No.-

The present paper investigates the chatter stability of a milling spindle-workpiece system in face milling process. To this end, a new method using frequendy response function is adopted for predicting the chatter vibration. A compliant workpiece is used to make the spindle-workpiece system easily exposed to chatter vibration. The entire experimental procedure, including measurement of frequency response function and cutting coefficients, is demonstrated. The experimental result shows that the chatter stability prediction method using frequendy response function is of great use in face milling process.

노화와 간경변이 인체의 적혈구, 혈장 및 위 점막의 Glutathione 농도와 Glutathione Peroxidase 활성도에 대해 미치는 영향

백광호,김종혁,백일현,한태호,박현주,박상훈,서중산,박충기,유재영 대한노인병학회 2001 대한노인병학회지 Vol.5 No.3

법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능

박종태,최용석,김흥중,정문진,오현주,신인철,박주철,손호현 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.6

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin,MMP2 mRNA 발현이 감소하였으며, CM-OD314를 transfection하여 OD314의 과발현을 유도한 경우에는 OD314와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다. This study was aimed to elucidate the biological function of OD314 (Apin protein), which is related to ameloblast differentiation and amelogenesis. Apin protein, calcifying epithelial odontogenic (pindborg) tumors (CEOTs)-associated amyloid, were isolated from CEOTs, and has similar nucleotide sequences to OD314. We examined expression of the OD314 mRNA using in-situ hybridization during tooth development in mice. Expression of OD314 and several enamel matrix proteins were examined in the cultured ameloblast cell line up to 28 days by reverse transcription-polymerase chain reaction (RT-PCR) amplification. After inactivation and over-expression of the OD314 gene in ameloblast cell lines using U6 vector-driven RNA interference and CMV-OD314 construct, RT-PCR were performed to evaluate the effect of the OD314 during amelogenesis. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were more strongly expressed in ameloblast than odontoblast. 2. When ameloblast cells were cultured in the differentiation and mineralization medium for 28 days, the tuftelin mRNA expression was maintained from the beginning to day 14, and then gradually decreased to day 28. The expressions of amelogenin and enamelin were gradually decreased according to the ameloblast differentiation. 3. Inactivation of OD314 by U6-OD314 siRNA construct down-regulated the expression of OD314, MMP-20, and tuftelin, whereas over-expression of OD314 by CMV-OD314 construct up-regulated the expression of OD314 and MMP-20 without change in tuftelin. These results suggest that OD314 is considered as an ameloblast-enriched gene and may play the important roles in ameloblast differentiation and mineralization.

아동기에 그룹홈에서 성장한 보육사에 대한 체험 내러티브 탐구

박현정,정익중 이화여자대학교 사회복지전문대학원 사회복지연구소 2022 사회복지 실천과 연구 Vol.19 No.3

본 연구의 목적은 아동기에 그룹홈에서 성장하였고 현재 성인이 되어 그룹홈에서 일하는 보육사 3명의 체험을 탐색하고 이해하는 것이다. 이를 위해 경험에 의미를 부여하는 Clandinin과 Connelly의 내러티브 탐구를 활용하였다. 연구참여자들이 경험한 삶의 의미는 “바위틈 노간주나무 씨앗이 먼저 뿌리내린 후 후일에 찾아온 진달래꽃 씨앗이 뿌리내리도록 돕다”로 도출되었다. 아동기에 그룹홈에서 성장한 보육사를 살펴본 연구 결과를 통해, 취약한 그룹홈 아동과 종사자를 지원할 수 있는 사회복지 실천과 정책에 대한 함의를 논의하였다. The purpose of this study was to understand experiences of three care workers who grew up in a group home during childhood. To this end, this study used Clandinin and Connelly (2000)’s narrative inquiry known to bestow meanings to experience. The meaning of life experienced by study participants was derived from the metaphorical phrase “The seeds of Juniperus rigida in the rocks take root first, and then help the seeds of azaleas that come later to take root.” Based on study results, the implications of social welfare practice and policy that can support vulnerable children and workers in group homes were discussed.

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

무기염류의 첨가가 김치성분의 변화에 미치는 영향

박상현,신중엽,박계인 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-

This study was to investigate the effects of addition of inorganic salts (KCl, MgCl_(2).6H_(2)O, CaCl_(2), 2H_(2)O) on the change of kimchi components. To this end, kimchi was prepared by adding inorganic salts and fermented at low (6-10℃) and high (20-22℃) temperatures. pH, acidity and pectic substances of kimchi were analyzed. During fermentation, pH, AIS (Alcohol insoluble solid), and HCl soluble pectin (HClSP) were decreased, while acidity and hot water soluble pectin (HWSP) were increased. These changes were more remarkable at high temperature than low temperature. The addition of inorganic salts in kimchi preparation influenced the changes of pH, acidity, HWSP, and HClSP at both low and high temperatures. The changes of pH and acidity were more remarkable in kimchi, prepared with addition of MgCl_(2) .6H_(2)O or CaCl_(2) .2H_(2)O than KCl. The changes of HWSP and HClSP were more marked in kimchi, which was added with KCl than MgCl_(2). 6H_(2)O or CaCl_(2) .2H_(2)O

사람의 비후성 반흔에서 면역조직화학기법에 의한 형질전환인자 알파와 표피성장인자 발현의 차이에 관한 면역조직화학적 연구

박영란,김현곤,김종중,문정석,송준섭,설경수,정윤영 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background and Objectives: Transforming growth factor-α (TGF-α) and epidermal growth factor (EGF) are polypeptides which interact with the epidermal growth factor receptor (EGFR) to produce their biological effects. The aim of the present investigation was to elucidate the immunolocalization of TGF-α and EGF in normal human skin, hypertrophic scar with skin graft on soft tissue defect, and postburn hypertrophic scar without graft. Methods: The data presented in this paper focused attention on differences of expression between two kinds of hypertrophic scars in relation to skin graft using immunohistochemistry. Formalin-fixed and paraffin-embedded tissues from 3 normal skin tissues, 3 hypertrophic scars after skin graft and 8 postburn hypertrophic scars without graft were immunolabelled with antibodies directed against TGF-α and EGF. Results: In normal epidermis of skin, strong TGF-α immunoreactivity (IR) was observed in all epidermal layers except the stratum (S.) basale, whereas EGF was immunopositive in a few cells over all epidermal layers. The staining for TGF-α was found in cell membrane, and EGF was seen either diffuse cytoplasmic or peripheral part of cells in normal epidermis. In hypertrophic scar after six months postoperation, TGF-α IR was observed in the upper part of S. spinosum and S. corneum, whereas EGF was diffusely expressed in the S. spinosum, also its intensity was slightly increased compared to normal skin. In hypertrophic scar without skin graft, very strong expression of EGF was detected in the S. spinosum and S. corneum, and intensity of EGF was increased when compared to normal skin and postoperative hypertrophic scar. EGF and TGF-α coexisted in the cells of S. spinosum in normal skin as well as hypertrophic scars. Double-labeled cells were increased in postburn hypertrophic scar compared to normal skin and postoperative hypertrophic scar. In addition, intensity of EGF and TGF-α double IR was the strongest in postburn hypertrophic scar. Conclusion: These results indicate that EGF may have a more complex regulatory role not only in the early stages of wound healing but also in hypertrophic scar.

Helicobacter pylori 와 대장의 신생물과의 연관성

안중욱,류재현,김광호,박응범 대한대장항문학회 2001 Annals of Coloproctolgy Vol.17 No.3