별늑대거미(Pardosa astrigera L. KOCH)후측안의 미세구조

정문진,윤명진 한국거미연구소 1992 한국거미 Vol.8 No.1,2

무당개구리 (Bombina orientalis) 피부의 초기 상처치유반응

정문진,문명진,Jeong, Moon-Jin,Moon, Myung-Jin 한국현미경학회 1998 Applied microscopy Vol.28 No.3

Early wound healing responses in the skin of the Korean fire bellied toad, Bombina orientalis, were examined using histochemical , scanning and transmission electron microscopical techniques. Cutaneous wounds were induced by sharp razor blade , and maintained in special cages for up to 3 days. By this treatment whole parts of epithelium and dermis were profoundly affected. After injury, retraction of damaged surface of the skin was observed. At 3 hours after injury, poly-band was form by mucous substance. The wound skin consisted of mucous gland secreted mucous substance which flows into wound cavity and the wound area was sealed by mucous substance which form thick layer at 6 hours. It appeared that natural disposition showed acid and neutral mucous substance by reaction of PAS and Alcian blue (pH 2.5) staining. After formation of the poly-band, epithelial cells were formed by migration of regenerating epithelial cells. These cells moved over the wound surface by migration at 12 hours after wounding. Poly-band was much increased in thickness and migrating cells were completely formed wound closure at 24 hours post-wounding. After 72 hours regenerated epithelial layer consists of four or five cell layers which are similar to clear, granular, and spinous layer.

별늑대거미(Pardosa astrigera (L.) Koch) 전중안 망막 시세포의미세구조

정문진,문명진 檀國大學校 基礎科學硏究所 1995 論文集 Vol.6 No.-

상처치유 과정에서 아미노산 수송계 L의 발현

정문진,김창현,최백동,정순정,박주철,김흥중,박경진,김도경 대한체질인류학회 2006 해부·생물인류학 (Anat Biol Anthropol) Vol.19 No.1

The continuous growth and proliferation of cells are essential for the wound healing process, and the amino acid transporters plays an important role in the continuous growing and proliferating cells. Among the amino acid transport systems, the amino acid transport system L, which is a Na+-independent neutral amino acid transport system, is a major route for providing living cells including tumor cells with neutral amino acids including several essential amino acids. In the present study, to elucidate the role of amino acid transport system L in the wound healing process, we investigated the expression pattern of LAT1 and LAT2 in the healing process after inflicting the wound on skin of rat. The expression of LAT1 was increased at 12 hours after inflicting the wound and was similar to the control group getting closer to 7 days. The expression of LAT2 was increased at 1 day and 3 days after inflicting the wound and was similar to the control group getting closer to 7 days. These results suggest that the LAT1 and LAT2 play important roles at the early stage and at the middle stage getting closer to normal skin in the wound healing process after inflicting the wound, respectively. 상처치유 과정에는 계속적인 세포성장과 증식이 필수적이며, 이러한 과정에 영양물질 수송체의 역할은 필수적이라고 할 수 있다. 아미노산 수송계 L은 중성아미노산을 수송하는 세포막 단백질로서 종양세포를 포함한 대부분의 세포에서 중성아미노산의 주 경로가 되는 아미노산 수송계로 알려져 있다. 본 연구는 상처를 유발시킨 흰쥐를 이용하여 상처치유 과정에서 아미노산 수송계 L의 발현양상 및 역할을 밝히고자 하였다. 동일조건 하에서 일정기간 사육한 흰쥐의 피부에 상처를 유발시킨 후, 12시간, 1일, 3일, 5일, 7일째에 조직을 적출하여 역전사-중합효소 연쇄반응과 면역조직화학적 분석 등을 이용하여 상처치유 과정에서 아미노산 수송계 L의 발현을 분석하였다. LAT1의 발현은 상처유발 후 12시간째에 증가가 있었으며, 1일, 3일, 5일 및 7일째로 경과하면서 대조군과 점차 유사해졌다. LAT2의 발현은 상처유발 후 1일과 3일까지 증가하였으며, 5일과 7일째로 경과하면서 대조군과 점차 유사해졌다. 본 연구의 결과로서 중성아미노산 수송계 L 중, LAT1은 세포손상 후 상처치유과정의 초기단계에서 중요한 역할을 하며, LAT2는 상처치유 기간에서 점차 정상으로 진행되어가는 중기단계에서 중요한 역할을 하는 것으로 사료된다.

Effect of rosmarinic acid on differentiation and mineralization of MC3T3-E1 osteoblastic cells on titanium surface

정문진,임도선,김성옥,박철,최영현,정순정 한국통합생물학회 2021 Animal cells and systems Vol.25 No.1

Titanium (Ti) is a widely used biomaterial for dental implants because of its outstanding biocompatibility for hard tissues. Osseointegration, the interaction between implanted biomaterials and living cells in bone, is essential for successful implantation. Rosmarinic acid (RA) is a plant-derived phytochemical with low toxicity and side effects and has various effects that can be applied as a therapeutic substance. The MC3T3-E1 osteoblastic cells on the Ti surface in medium with or without 14 μg/ml RA were used to test RA effects on osteoblast differentiation, cell viability and mineralization during differentiation. RA treatment increased osteoblast differentiation, cell viability and mineralization in MC3T3-E1 osteoblastic cells on Ti surface during differentiation, upregulating Runx-2 and OPG, but downregulating RANKL. This study suggest that RA should be applied as an effective functional and therapeutic substance to enhance osseointegration of osteoblast cells by increasing differentiation, mineralization, and bone formation through the RANKL/RANK/OPG pathway during the differentiation in MC3T3-E1 osteoblastic cells on the Ti surface.

Bombina orientalis 피부손상 후 과립선 재생에 관한 미세구조적 연구

정문진,문명진,임도선 한국현미경학회 2002 Applied microscopy Vol.32 No.3

Granular gland regeneration in the toad after dorsal skin wound histologically was examined using scanning and transmission electron microscopy. After cutaneous wounds were induced by excision, animals were maintained in special cages for up to 20 days. In transmission electron microscopy (TEM), newly formed granular gland, though poorly developed, was seen on 4 day after injury. Epithelial cells moved toward apical region of newly formed gland. The cells had smooth surface and were not connected to other cells by desmosomes. Mitochondria rich cell (MRC) possessing long cytoplasmic processes formed a gland cavity and hemidesmosomes were found under the cell processes. Basal cavity of newly formed gland consisted of MRC, pro-granular producing cells (pGPC), and granular producing cell (GPC). Moreover it was observed that xanthophores moved to the base of the epithelial tissue on 10 day after the injury. These cells contained numerous pterinosomes and carotenoid vesicles. Immature pterinosomes were large and carotenoid vesicles were moderately electron dense. On 13 day after the injury, xanthophores contained abundant carotinoid vesicles and lammelated pterinosomes. Iridophores were also observed adjacent the developing xanthophores on 16 day post-injury. These observations indicated that regeneration of granular gland from glandular precursor cells during wound healing and subsequent expansion of the glandular cells might be dependent on maturation and proliferation of these newly formed cells. 두꺼비 등 피부손상 후 과립선의 재생과정을 주사전자현미경과 투과전자현미경으로 관찰하였다. 절개에 의하여 피부손상을 가한 후 실험을 위하여 특수히 제작된 cage 내에서 최대 20일 간 사육하였다. 투과전자현미경 관찰에서 손상 4일 후 미성숙 형태의 신생 과립선이 관찰되었으며, 상피세포는 신생 과립선의 첨단부로 이동하여 있었다. 상피세포의 표면은 편평하였으며 desmosome에 의해 서로 연결되어 있지 않았다. 미토콘드리아를 많이 함유한 세포돌기(MRC)들이 선의 내강을 형성하고 있었고, 이들 돌기에서는 hemidesmosome이 관찰되었다. 신생선의 기저강은 MRC, 과립형성전세포, 및 과립형성세포 등으로 이루어져 있었다. 특히, 손상후 10일에 xanthophore가 상피세포의 기저부로 이동하여 있음이 관찰되었다. 이들 세포는 다수의 크기가 큰 pterinosome과 중등도의 전자밀도를 가진 carotenoid vesicle을 포함하고 있었다. 손상후 13일에, xanthophore는 많은 carotinoid vesicle과 lammelated pterinosomes을 포함하고 있었다. Iridophore는 손상 16일에 분화중인 xanthophore 주변에서 관찰되었다. 이러한 소견은 손상으로부터의 회복 과정에 선조세포(glandular precursor cell)로부터 과립선이 재생되며, 선세포의 팽대는 이들 신생 세포의 성숙 및 증식에 의한 것을 의미하는 것을 의미한다.

Protection of Oxidative Stress-induced DNA Damage and Apoptosis by Rosmarinic Acid in Murine Myoblast C2C12 Cells

정문진,임도선,김성옥,박철,임선희,이혜숙,김기영,정순정,최영현 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.2

Rosmarinic acid (RA) is a naturally occurring polyphenolic compound that has been applied as a therapeutic antioxidant. However, its action mechanism in muscle cells remains unclear. The objective of the current study was to explore the protective effect of RA against DNA damage and apoptotic cell death caused by hydrogen peroxide (H2O2) in mouse C2C12 myoblast cells and clarify related signaling pathway. Our results showed that pretreatment with RA significantly improved cell viability, inhibited apoptosis, and suppressed the generation of reactive oxygen species (ROS) in H2O2-treated C2C12 cells. Additionally, DNA damage triggered by H2O2 was abrogated by RA pretreatment. Moreover, H2O2-induced mitochondrial dysfunctions associated with apoptotic events, including loss of mitochondrial membrane potential and adenosine triphosphate content, up-regulation of Bax, down-regulation of Bcl-2, and cytosolic release of cytochrome c, were reduced in the presence of RA. RA also attenuated H2O2-induced activation of caspase-9 and caspase-3, which was associated with the ability of RA to block the degradation of poly (ADP-ribose) polymerase. Furthermore, RA dramatically promoted nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and its phosphorylation in the presence of H2O2 with concomitant upregulation of heme oxygenase-1 (HO-1) expression, a major downstream factor of Nrf2. Taken together, these results suggest that RA could protect C2C12 myoblasts from H2O2-induced damage by maintaining mitochondrial function while eliminating ROS along with activation of the Nrf2/HO-1 antioxidant pathway, indicating that RA may be a potential therapeutic candidate to treat oxidative stress-mediated injury.

Comparative Morphological Study on Parotid and Submandibular Salivary Glands in Ovariectomized Rats

정문진,Myoung-Hwa Lee,임도선,Myeongju Jeong,Soon-Jeong Jeong 한국치위생과학회 2022 치위생과학회지 Vol.22 No.2

Background: Estrogen deficiency affects the structure and function of the salivary glands in women, leading to a decrease in salivary secretion and a change in the composition of saliva. Previous studies on changes in the salivary glands that cause estrogen deficiency have reported only partial results for the parotid and submandibular glands, and there are few comparative morphological studies of histological changes between the parotid and submandibular glands in ovariectomized rats (OVX) leading to estrogen deficiency. This study aimed to analyze the histopathological and histochemical changes in the parotid and submandibular salivary glands causing estrogen deficiency by using OVX, and to discuss the mechanism on these changes.Methods: The parotid and submandibular glands from sacrificed control and OVX groups were fixed with cold 4% paraformaldehyde in phosphate buffer (pH 7.2). The tissues were dehydrated using a series of graded ethyl alcohol and embedded in paraffin. For histopathological analysis, sections cut to a thickness of 6 to 7 µm were stained with hematoxylin and eosin (H&E). For histochemical analysis, Periodic acid-Schiff (PAS), Alcian blue (AB, pH 2.5), and PAS+AB (pH 2.5 and pH 1) staining was performed.Results: Histopathological analysis of OVX tissue showed that the parotid and submandibular salivary glands were broadly and clearly separated and divided into lobes. In OVX, acinar and ductal cells with condensed polymorphic or pyknotic nucleus, which are presumed to be characteristic of apoptotic cells, and degenerated cells with lipid deposition in cytoplasmic granules and ruptured membranes were increased. Histochemical analysis of OVX, confirmed an increase in the number and acidification of acinar secretory granules.Conclusion: Histopathological and histochemical changes and the effects of estrogen deficiency are more evident in the submandibular salivary gland than in the parotid gland.

Biological Effects of Light-Emitting Diodes Curing Unit on MDPC-23 Cells and Lipopolysaccharide Stimulated MDPC-23 Cells

정문진,Soon-Jeong Jeong 한국치위생과학회 2019 치위생과학회지 Vol.19 No.1

Background: Light-emitting diodes curing unit (LCU), which emit blue light, is used for polymerization of composite resins in many dentistry. Although the use of LCU for light-cured composite resin polymerization is considered safe, it is still controversial whether it can directly or indirectly have harmful biological influences on oral tissues. The aim of this study was to elucidate the biological effects of LCU in wavelengths ranging from 440 to 490 nm, on the cell viability and secretion of inflammatory cytokines in MDPC-23 odontoblastic cells and inflammatory-induced MDPC-23 cells by lipopolysaccharide (LPS). Methods: The MTT assay and observation using microscope were performed on MDPC-23 cells to investigate the cell viability and cytotoxic effects on LCU irradiation. Results: MDPC-23 cells and LPS stimulated MDPC-23 cells were found to have no effects on cell viability and cell morphology in the LCU irradiation. Nitric oxide (NO) and prostaglandin E2 which are the pro-inflammatory mediators, and interleukin-1 and tumor necrosis factor- (TNF-) which are the proinflammatory cytokines were significantly increased in MCPD-23 cells after LCU irradiation as time increased in comparison with the control. LCU irradiation has the potential to induce inflammation or biological damages in normal dental tissues, including MDPC-23 cells. Conclusion: Therefore, it is necessary to limit the use of LCU except for the appropriate dose and irradiation time. In addition, LCU irradiation of inflammatory-induced MDPC-23 cells by LPS was reduced the secretion of NO compared to the LPS alone treatment group and was significantly reduced the secretion of TNF- in all the time groups. Therefore, LCU application in LPS stimulated MDPC-23 odontoblastic cells has a photodynamic therapy like effect as well as inflammation relief.

Comparing Chewable and Manual Toothbrushes for Reducing Dental Plaque: A Pilot Study

정문진,신혜선,정순정,임도선 한국치위생과학회 2017 치위생과학회지 Vol.17 No.3

This study aimed to compare the effectiveness of chewable toothbrush and manual toothbrush and provide basic data for recommendation of the chewable toothbrush in specific groups and situations. A total of 20 subjects participated in this study (rolling method, 10; non-rolling method, 10). After professional prophylaxis, participants used the manual toothbrush to brush their teeth for 3 minutes. After a 7-day wash-out period, participants used the chewable toothbrush according to the manufacturer’s instructions. Pre- and post-plaque indexing of the teeth was performed. The dental plaque index was assessed using the Turesky Modification of the Quigley-Hein Plaque Index (TMQHPI) for amount of plaque and Silness-Löe Plaque Index (SLPI) for plaque thickness. The difference between pre- and post-dental plaque index was analyzed using a paired t-test and the Wilcoxon signed-rank test. The Mann-Whitney U test was also used to compare the dental plaque index reduction rates. The dental plaque index differed significantly between the chewable toothbrush and the manual toothbrush. The TMQHPI reduction rate was significantly different between the rolling and non-rolling method groups for the manual toothbrush but not the chewable toothbrush. The difference in SLPI reduction rate between the rolling and non-rolling method groups was significant for the manual toothbrush but not for the chewable toothbrush. Differences in the dental plaque index reduction rates between the chewable and manual toothbrushes were not significant in the non-rolling method group. The results of this study showed higher reduction rates in dental plaque with manual toothbrush use than with chewable toothbrush use. However, the non-rolling method group did not show statistically significant differences according to toothbrush type. The present study showed that a chewable toothbrush can be an alternative to a manual toothbrush for individuals who have difficulty using the generally recommended rolling method.