Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

탐구형 기하 소프트웨어 환경에서 피드백을 활용한 증명학습에 관한 연구

류희영,조민식 한국교원대학교교육연구원 2006 敎員敎育 Vol.22 No.3

탐구형 기하 소프트웨어의 환경에서 기하교육이 유용하다는 많은 연구 결과가 있었다. 본 연구에서는 소프트웨어의 환경에서 교사가 적절한 피드백을 주었을 때 작도문제를 통한 증명학습에 어떠한 영향을 주는지에 대하여 분석하였다. 그 결과 교사는 학생들의 수준을 적절히 판단하여 적합한 피드백을 줄 수 있고 학생들의 사고력의 향상과 지속성을 관찰할 수 있었고 학습태도에 긍정적인 영향을 끼치는 것으로 나타났다. The purpose of this study is to investigate, through case study, how the feedback with dynamic geometry software has influence on the improvement of proof learning and student's attitude. Analysis of data including worksheets, computer activities, and interviews revealed that collaborative learning is enhanced and student's attitudes could be changed positively.

인후두부 이물감을 호소하는 환자에서의 기질적 요인 및 심인성 요인의 중요성

손영익,권중근,이풍렬,이화영,유범희,김지혜 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.3

형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량

유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.

김치식이가 흰쥐의 항산화시스템에 미치는 영향

류승희,권명자,송영옥,송영선,문갑순 인제대학교 2003 仁濟論叢 Vol.18 No.1

김치는 배추를 주재료로 하여 마늘, 고추, 생강 등의 양념류를 포함하는 여러 재료들로 만들어지며 이들 재료 중에는 항산화효과를 가진 것으로 알려진 성분들이 많이 함유되어 있어 김치도 항산화효과를 나타낼 것으로 여겨진다. In vitro 실험계 및 세포 모델계에서 김치 재료 및 김치의 항산화효과는 확인된바 있으나 In vivo 시스템에서의 김치의 항산화효과에 관한 자료는 부족하다. 따라서 본 실험에서는 김치 섭취의 생체 내 항산화효과를 규명하기 위하여 흰쥐에게 동결건조한 김치 3, 5, 10% 첨가 식이를 6주간 섭취시키면서 간조직의 산화와 항산화효소계의 활성을 측정하였다. 간의 MDA 함량은 대조군에 비해 김치 투여군에서 낮았고 특히 5% 김치섭취군에서 현저히 낮은 값을 나타내었다. 단백질 산화를 나타내는 카르보닐 함량도 대조군에 비해 김치섭취군에서 낮아 생체 내에서 김치식이가 과산화물 생성 억제효과를 나타내었다. 항산화 효소계의 활성을 측정한 결과 SOD 활성은 대조군에 비해 김치섭취군에서 현저하게 높았고 김치첨가 함량이 증가할수록 SOD 활성이 유의적으로 높아져 10% 김치섭취군에서는 대조군에 비해 70% 이상 높은 활성을 나타내었다. Catalase 활성은 각 군에서 유의적인 차이가 나타나지 않았으나 GSH-px의 활성은 김치 섭취가 높을수록 상대적으로 높은 증가율을 나타내어 SOD 활성과 동일한 경향을 나타내었다. 따라서 김치식이는 생체 내에서 치질 및 단백질 과산화를 현저하게 억제하였고 항산화효소계인 SOD와 GSH-px의 활성을 농도의존적으로 증진시켜 김치식이가 체내의 산화적 스트레스를 효과적으로 억제하는데 기여함을 알 수 있었다. Kimchi is a Kruean traditional fermented food containing various ingredients such as Chinese cabbage, garlic, ginger, red pepper and fish sauce. Some of their components were known as its antioxidative activities and antioxidative effects of kimchi have been studied using in vitro and cell model system. To investigate the antioxidative effect of dietary kimchi, 4 groups of male S.D. rats (n=36) were fed 0, 3%, 5%, and 10% freeze-dried kimchi for 6 weeks and hepatic lipid peroxidation, protein oxidation and the activities of antioxidative enzymes were measured. Hepatic MDA content fed kimchi diet was lower than control diet. Protein carbonyl values were decreased in kimchi diet group, but not significant. SOD activities were significantly increased in kimchi added groups at dose-dependent manner. At 10% kimchi added group, SOD activity increased by 70% compared with control diet group. Catalase activities were not different between experimental groups and control group, whereas GSH-px activities were significantly increased in kimchi added group. As a result kimchi diet inhibited lipid and protein oxidation and induced antioxidative enzymes, SOD and GSH-px in rat hepatic tissue. It suggests that dietary kimchi is expected to prevent the oxidative stress in animal and human.

콩 삶은 물을 첨가한 청국장 제조시 항산화활성 및 관련 성분들의 변화

이경희,류승희,이영순,김영만,문갑순 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.2

Soybean is an important plant as the source of protein and oil, as well as phytochemicals such as genistein, daidzein, phenolic acids, phytic acid, tocopherol, and saponin. Chungkukjang, a fermented soybean paste, is cammon in Korean meals and bacillus is usually used in the fermentation of steamed soybean. For its processing, whole soybeans are boiled in water until the beams are soft, and then the drained beans are wrapped with rice straw or starter and set in a warm place at 65℃ for 3~4 days. Normally, the remaining cooked water which was drained from the steamed beans is discarded. We supposed that this water possesses high amounts of useful components, and we therefore developed a modified method using the cooked water. After fermentation, we added the remaining cooked water which had been drained from the beans to the fermented soy beans and boiled them together. To investigate the bio-functionality of the modified chusngkukjang, the total antioxidative activity, isoflavones contents, phenolic acids, and 3-deoxyglucosone (3-DG) were measured at each stage of the preparation of changkukjang, The original and modified chturgkukjang possessed l a high antioxidative activity compared with the other samples, as did the drained water after steaming of the soybean. The contents of genistein, daidzein, and phenolic acids, which contained antioxidative activity, were also increased in the original chungkukjang and their contents were similar in the modified chungkukjang. The content of 3-DG was increased in the modified chungkukjang compare with the original. It is suggested that the active soybean components delivered to the drained water during the steaming process were useful for increasing the bio-functionality of the modified chunghnkjang.

충북지역 도시방재계획의 도시계획적 진단과 발전방향 : 도시계획서 분석 및 전문가 의견조사를 중심으로

백기영,황희연,박병호,이만형,황재훈,류을렬 永同大學校 1999 硏究論叢 Vol.5 No.1

본 연구는 도시방재계획의 현황을 도시계획적 차원에서 진단하고 그 발전방향을 도출하고자 하는 목적하에, 충청북도의 조례와 지침중 도시방재 관련 사항을 정리하며, 충청북도 지방도시계획위원회 회의록 및 관련자료를 검토하여 방계계획의 취급 수준을 파악하였다. 실제 도시계획 상황에 대한 진단은 도시계획 보고서를 대상으로 도시방재계획의 위상을 분석하고 평가하였다. 또한 도시방재계획의 위상과 관련 전문가의 인식수준을 평가하고 이를 바탕으로 향후 바람직한 도시방재계획의 위상을 설정하기 위해 필요한 사항을 분석하고자, 도시방재에 관한 일반의식 수준, 도시재해에 관한 대처방안, 도시방재계획의 평가방향 등을 중심으로 설문조사를 실시하였다. 이상과 같은 충북지역의 방재계획에 대한 도시계획적 진단을 바탕으로 도시방재계획 관리체계의 방향 도출과 도시방재계획의 향후방향 제안을 시도하였다. This paper examines the status quo of the existing urban disaster prevention plans in Chungbuk Province and suggests alternative orientations for the future application. Its methodological themes center around the systematic compilation and accountability of disaster-related ordinances, mostly hinged on evaluation of major disaster items documented in various urban master or revision plans. In addition, it recommends the concrete guidelines and contents in disaster prevention plans, both of which are based on the aggregated results of expert opinion survey in and around Chungbuk Province. In order to tackle with diverse and unpredictable disasters which are well observed in modern urban society, this paper urges that the relatively weak legal and planning standards of the existing disaster prevention plans have to be immediately revised. For instance, the paper vividly shows that the present dimension of disaster prevention plans within the existing master plans and perception of specialist are not satisfactory mainly because of the internal limitation of the existing plans. Secondly, it seems imperative for planners to pay attention to systematic operation and management, even starting from the early development stage of urban disasters. Lastly, it proposes the alternative checklists given to measure the suitability of the disaster plans.

평균대 경기 시 앞 공중돌기 동작에 대한 운동학적 분석

윤희중,류지선,김동민,김영란 한국운동역학회 2000 한국운동역학회지 Vol.9 No.2

본 연구는 평균대에서 수행되는 앞 공중돌기 동작의 효율적인 동작을 연기하는데 필요한 정보를 제공하기 위한 운동학적 요인을 살펴보는 데 있다. 연구대상은 부산동아시아 체조경기대회(1997년)평균대 종목별 결승에 참가한 한국선수 1명, 카자흐스탄 2명, 일본선수 1명을 대상으로 3차원 영상 분석을 통하여 운동학적 요인을 분석한 결과 다음과 같은 결론을 얻었다. 1. 각국면별 소요시간 도움닫기 시간은 스텝시간이 대상자별로 다르게 실시함으로서 수행시간간에 차이를 보였다. 도약 시간은 선행연구 결과에 비해 본 연구의 대상자들이 보다 짧은 시간을 나타냈으며, 체공시간은 길게 소요하고 있는 것으로 나타났다. 2. 체공 및 착자시 인체중심 높이는 선행연구된 마루 운동의 수행 결과들 보다는 다소 낮게 나타났다. 3. 도약시 인체중심 속도는 수직속도보다 수평속도가 높게 나타났다. 4. 도약 및 착지시 선정된 각 관절의 각도는 선행연구 결과들 보다 무릎각을 제외한 발목, 고관절 각도에서 적은 각도를 나타내 불안정한 착지를 수행하는 것으로 나타났다. The purpose of this study was to analyze the kinematic variable of a forward somersault motion in balance beam. Two video cameras were used for 3-dimentional analysis. The subjects were four female gymnasts who is participated in the '97 east asian game. The conclusions are follows. 1. Time of each phase The time of run-up was shown a difference between performance time as type of step patterns of each subjects. The time of support was display a more short time in the gymnasts of present study compared to gymnasts of previous study, and it was shown a long time in the flight phase. 3. The velocity of center of gravity in supports phase was shown more high levels in horizontal velocity than vertical velocity. 4. The angle of each joint in support and landing was display a low angles in ankle and hip joint angles except to knee joint angles. Therefore, it was performing a unbalanced landing.

生長調節物質의 處理가 고추의 着果 및 果實特性에 미치는 影響

金永信,呂承淑,李禧珠,李政明 경희대학교 부설 식량자원개발연구소 1991 硏究論文集 Vol.12 No.-

A series of experiments were conducted to investigate the effect of some growth regulator treatment on the fruit set and fruit growth in a male sterile inbred line of hot pepper(Capsicum annuum ) which normally bears bean-sized tiny fruits only. Major results can be summarized as follows. 1. In the first experiment, which was consisted of Tomtlane treatments alone or in combination with gibberellic acid, fruit growth was not significantly influenced by any of the treatments. Only the hand-pollinated flowers with viable pollens from other fertile plants gave rise to significantly larger fruits suggesting that competition for nutrients within a plant may reduce the effectiveness of the chemicals treated. 2. 2, 4-D treatment, dipping the flowers in l00ppm solution, was found to be the most effective in inducing parthenocarpic fruit enlargement as compared to other chemicals in the second experiment. 3. In the 3rd experiment, which included all the treatments tested in the first and second experiments, 2,4-D treatment was found to be the most effective followed closely by Tomatotone (p-CPA) treatment. 4. Addition of gibberellic acid to Tomatotone appeared to increase the effectiviness of Tomatotone treatment. Addition of gibberellic acid to NAA or Tomatlane failed to show any significant additive effect. 5. Highly significant postitive correlation was found between the fruit fresh weight and the fresh weight of the placentae tissue thus suggesting the important role of placentae tissue in enlargement of fertilized fruits.

배재대학 부속 유치원의 통합적 교육과정 모형 개발에 관한 일 연구

오영희,류칠선,이성희 培材大學校 1996 培材論叢 Vol.1 No.-

This paper presented the structure and the moldel of the integrate educational program accout for the perception of the parents and the developmental level of the children of the Pai Chai University's laboratory Kindergarden. The model and components of the presented program were as follows. First, the purpose and the goal of the program for the children were "enhancing the intergaral development" and "enhancing the helthy disposition to be a good citizen." And the goal for the parents were "keeping a close connection between kindergarden and home." The purpose and the goal of the program were estiblished by the Christian faith, the education goal of the Taijeon Board of Education, and the result of the survey on the need of parent and early childhood education specialists. Second, the contents of the program enclose the areas of "Helth", "Social", "Expression", "Language", "Inquiry", "Religon", and "Livelihood habit" to enhance the intergaral development and the helthy disposition to be a good citizen. For the parents, the program provided the "small books" and "inviting lectures". Third, the teaching methods for the children focused on "running the learning centers", "daily plan to enhance the ability of presentation and thought". The teaching methods for the parent comprised "parent meeting", "interview with parents", "parent letters", "home visiting", and "lending books". Forth, the evelution of the program enclosed "the program evelution", "the development of the children evelution" and "steff evelution".