흰쥐 안면신경핵 세포의 전기생리학적 및 형태학적 특성

최병주,조진화,배용철,김영진 大韓小兒齒科學會 2000 大韓小兒齒科學會誌 Vol.27 No.3

흰쥐의 안면신경핵을 구성하는 신경세포들의 시냅스 연결 양태 및 세포막 특성을 규명하기 위해 in vivo 필드전위 및 세포내 전위 측정법을 이용하여 전기생리적 반응을 관찰하였다. 말초 안면신경 분지를 역행성으로 전기자극시 자극세기에 비례하여 전위의 크기가 증가되었고 필드 전위의 양태는 두 가지 반응으로 나타났는데 전기자극 직후 1ms 부근에서 정점을 나타내는 양태와 이와 더불어 7~8ms 부근에서 후기 정점을 동반하는 양태가 있었다. 안면신경핵은 염색시 내측, 배외측, 중간측 및 외측등 4부분의 소핵으로 구분되었다. Neurobiotin으로 채워진 단일 신경세포를 형태학적으로 재구축하였는데 세포체는 추체형태를 나타내었고 주 수상돌기는 모든 방향으로 뻗어져 있었고 각 수상돌기의 영역은 해당 소핵 내에 한정되어 있었다. 일련의 과분극 전류(-1.2~+1.2nA)를 세포내에 가하였을 때 동반되는 세포내 전위변화를 입력저항 값으로 계산하였을 때 그 기울기가 직선형으로 나타났다. 탈분극 전류를 세포내 주입시 지속적인 활동성 전위가 나타났으며 전류의 크기에 비례하여 각 전위의 개수가 증가하였고 spike-빈도 적응 현상이 나타났다. 그러나 시간 의존성 내향성 정류현상은 관찰되지 않았고 anodal break excitation이 나타났다. 이상의 실험결과로 보아 안면신경핵을 구성하고 있는 세포들 사이의 시냅스는 다양한 형태로 존재할 가능성이 있다고 사료되며 이들 시냅스간의 변화를 통하여 안면 신경마비, 반쪽 안면 경련, hypoglossal-facial anastomosis등에서 나타날 수 있는 임상적 신경성 증상 기전을 설명할 수 있을 것으로 여겨진다. 주요어 : 안면신경핵, 시냅스, 신경세포막 특성, 안면신경마비, 반쪽 안면 경련 This study used in vivo intracellular and extracellular fIeld potential recording to evaluate the intrinsic membrane properties and connection pattern within facial nucleus. 1.There were four subdivisions of medial, intermediate, lateral, and dorsolateral in facial nucleus. 2.Principal cells in the facial nucleus was recorded from and filled with neurobiotin in anesthetized rats. The extent of their dendrites and the characteristics of cell body were examined. 3.Principal cells had a large amplitude action potential and afterhyperpolarization was followed a single action potential. 4.The response from facial motonucleus to electrical stimulation of the facial nerve was mainly a monophasic wave, with a latency of 1 msec, which was assumed to reflect antidromic activation of facial motoneurons. In some of rats the response in addition showed late components at a latency of about 7-8 msec, but its amplitude was small. 5.Mest of cells exhibited accommodation of spike discharge upon depolarization of membrane by 0.8 nA for 400 ms. Our results support the hypothesis that there normally are weak connections between different parts of the facial motonucleus to explain pathophysiology of hemifacial spasm and facial nerve paralysis.

프레탈^(R)정(실로스타졸 50mg)에 대한 실로졸^(R)정의 생물학적 동등성

최한곤,권기철,이승호,김학미,박병주,유봉규,이종달,이경희,하정희,우종수,박인숙,최진석,용철순 한국병원약사회 2003 병원약사회지 Vol.20 No.1

Bioequivalence of two cilostazol tablets, the Pletaal^(R)(Korea Otsuka Pharmaceutical Co., Ltd.) and the Cilozol^(R)(Hanmi Pharmaceutical Co., Ltd.), was evaluated according to the guideline of KFDA, Sixteen normal male volunteers(age 20~29 years old) were divided into two groups and a randomized 22 cross-over study was employed. After two tablets containing 50㎎ of cilostazol were orally administered. blood was taken at predetermined time intervals and the concentration of cilostazol in plasma was determined with an HPLC method using UV detector. The pharmacokinetic parameters(C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters. The results showed that the differences in C_(max), T_(max) and AUC_(t) between two tablets were 4.99%, 1.74% and 7.68%, respectively. The powers(1-β) for C_(max), T_(max) and AUC_(t) were83.92%, 80.12% and 85.03%, respectively. Detectable differences(Δ) and confidence intervals were all less than 20%, and confidence interval of all the parameters were also less than 20% at the significance level(α) of 0.05. All of these parameters met the criteria of KFDA for bioequivalence, indicating that Cilozol^(R) tablet is bioequivalent to Pletaal^(R) tablet.

단기간 근권 저온처리가 수경재배 상추의 질산태 질소 함량에 미치는 영향

최승주,양진철,사동민 한국환경농학회 2002 한국환경농학회지 Vol.21 No.1

질산태 질소의 일중 농도 변화와 단기간의 저온 처리가 질산태 질소의 흡수 및 농도에 미치는 영향을 살펴보고자 상추(Lactuca sativa L.)를 공시 작물로 하여 온실에서 수경 재배하였다. 질산태 질소의 농도는 염산에 비하여 주맥에서 일중 지속적으로 2배 이상 높았으며, 질산태 질소의 일중 변화는 14:00까지 지속적으로 감소하여 최저치 (8.7 mg-N/GDW)를 나타낸 후 다시 증가하였다. 질산태 질소의 일중 흡수량은 11:00~17:00에 평균 4.8mg-N/GDW-Root/hr로 최대치를 나타내었다. 단기간의 저온 처리량을 50%~55%를 감소시켰다. 이러한 결과는 수확 전 단기간의 저온처리가 상추 잎의 질산태 질소함량을 낮출 수 있는 방법으로 응용될 수 있음을 보여준다. Lettuce (Lactuca sativa L.) plants were grown under hydroponic system to characterize the diurnal change of nitrate concentration and nitrate uptake rate and to examine the effect of short term cold treatment to rhizosphere on nitrate concentration and uptake rate in lettuce plant. The nitrate concentrations in midrib were two times higher than those in leaf. Nitrate concentration in the shoot reached to minimum (8.7 mg-N/GDW) at 14:00 and, thereafter, increased continuously until 23:00. During 11:00~17:00, nitrate uptake by lettuce plant was maximum (4.8 mg-N/GDW-Root/hr). Short term cold treatment reduced nitrate concentration in the shoot by 14~18%, and nitrate uptake rate by 50~55%, respectively. These results showed that short term cold treatment before harvest could be applied for the purpose of reduction of nitrate concentration in the leaf under hydroponic culture.

전산화단층사진을 이용한 하악골 비대칭 환자의 저작근 평가

최순철,이선복,이진구,이원진,허민석,이삼선 大韓口腔顎顔面 放射線學會 2004 Imaging Science in Dentistry Vol.34 No.1

Purpose : To compare the size of the masseter and lateral pterygoid muscle between the affected and the unaffected side of the patients who have the chief complaints of the mandibular asymmetry. Materials and Methods : Twenty two patients (male: 4, female: 18, average age: 21.3 year-old) were radiographed using posterior-anterior (P-A) cephalography and computed tomography (CT). On P-A cephalography, the degree of deviation was determined by the distance from the mentum to the vertical reference line through the crista galli and the anterior nasal spine. On the scanned tracing papers of the maximum cross-sectional area of the masseter and lateral pterygoid muscle using axial CT images, the pixel number was measured. The ratio of the affected : unaffected sides were obtained. For the masseter and lateral pterygoid muscle, the relationship between the muscular volume and degree of skeletal hypoplasia was studied. Results : The half cases showed no skeletal asymmetry. The lateral pterygoid muscle of the affected side was larger significantly than unaffected side (p<0.05). However, there was no significant difference between two sides in the cases of skeletal asymmetry. There was only significant difference in the cases without skeletal asymmetry (p<0.05). Conclusions : To some extent, the slight mandibular hypoplasia could affect the growth of some masticatory muscles.

Homocysteine과 대사증후군의 관계

최진욱,김선현,염창환,정규철 관동대학교 의과학연구소 2006 關東醫大學術誌 Vol.10 No.1

부적절한 예방화학요법 후 지연 발현된 Plasmodium vivax 말라리아 1 예

최수철,장상필,김상화,서환조,서진태,김구엽 대한내과학회 1998 대한내과학회지 Vol.54 No.3

Malaria is the world's most important parasitic infection. Although it has been erw5cated from temperate zones including Korea, increasing numbers of travellers visit tropical malarious countries and imported malaria becomes important medical problem in the developed countries. In Korea with increasing travellers to malaria-endemic area, the incidence of imported malaria shows rising tendency same as the developed countries. It becomes important to personal general personal protective measures and chemoprophylaxis to trevellers, when employed in appropriate manner, that can be highly effective in preventing malaria. We recently experienced a case of in Plasmodium vivax malaria with delayed manifestations due to inadequate chemoprophylaxis. A 53-year-old woman with history of trevel to East Africa 4 months ago and chief complaint of fever was diagnosed as tertian malaria. She had irregularly taken prophylatic antimalarial during travel and had not taken it after return but should have taken it at least 4 weeks after return.

혼합유의 이화학적 특성에 관한 연구

최성희,박철진,오성기 경희대학교 부설 식량자원개발연구소 1992 硏究論文集 Vol.13 No.-

The objective of this study was to investigate the physicochemical characteristics of binary and ternary oil systems. Materials used in this experiment were four edible oils i. e. sesame, soybean, red-pepper seed and Sancho (Zanthoxylum schinifolium) seed oils. The physicochemical properties of mixed oils, as well as the inductive period of oxidation, were measured. The results obtained are as follows : 1. The optimum mixing ratios of sesame oil with each of soybean, red-pepper seed and Sancho seed oils, which still maintained a characteristic sesame oil flavor, were 1 : 3,1 : 0.5 and 1 : 0.5, respectively. 2. Sesame, soybean and red-pepper seed oils showed low acid and peroxide values. In contrast, however, Sancho seed oil showed the very high values, and acid, peroxide and TBA values were 17. 4, 28.6 and 1.8, respectively, Among the oils, soybean oil gave the longest induction period of oxidation and Sancho seed oils the shortest, and the induction period was increased in order of Sanch seed, red-pepper seed, sesame and soybean oils. 3. Binary oil system such as a mixed oil of seasame oil and each of soybean and red-pepper seed oils did not show any significant change in acid, peroxide and TBA values, but a mixed oil of sesame oil and Sancho seed oils gave a drastic change in acid (7.2), peroxide (12.3), and TBA (0.2) values. All the binary oil systems extended their induction periods of oxidation. 4. In ternary oil system, a mixed oil of sesame, soybean and red-pepper sees oils did not show any significant change in acid and peroxide values. In contrast, however, mixed oils of sesame, red -pepper seed and Sancho seed oils in the ratios of 1 : 1 : 1 and 4.5, 8.9 and 0.13 in the ratio of 1 : 0.25 : 0.25, respectively. All the ternary oil systems also extended the induction period of oxidation.

하지방사통을 동반한 성인 협부결손성 요추전방전위증의 후방요추간유합술 및 추경나사고정술후 추적결과

최철,김근수,최하영,김철진,김형일,이정청 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.4

전유전체 서열 분석과 가시화를 위한 워크벤치 개발과 응용

최정현,진희정,김철민,장철훈,조환규 한국유전학회 2002 Genes & Genomics Vol.24 No.2

An increasing number of genome sequencing projects results in explosive growth of whole genome sequences. Furthermore the number of studies on the functions of individual genes has also been rapidly increased. However on-memory algorithms are not applicable to the analysis of whole genome sequences, since the size of individual whole gene a ranges from several million base pairs to hundreds billion base pairs. In order to effectively manipulate the huge sequence data, it is necessary to use the indexed data structure for external memory. In this paper, we introduce the development and application of the workbench for the analysis and visualization of whole genome sequences using string B-tree that is suitable for the analysis of huge data. This system consists of two main parts, the analysis query part and the visualization part. The query system supports various transactions such as pattern matching, k-occurrence, and k-mer analysis. The visualization system helps biologists to easily understand whole genome structure and specificity by various kinds of visualization such as whole genome sequence viewer, annotation viewer, CGR (Chaos Game Representation) viewer, k-mer viewer, RWP (Random Walk Plot) viewer, and map viewer. We can find the relationships among organisms, support gene prediction in a genome, and study the function of junk DNA using our workbench. In this paper, we apply our workbench to investigating specific sequence such as avoided sequence, common sequence, and classifiable sequence.

Goretex를 이용한 융비술

최진영,민철기,명훈,황순정,서병무,이종호,정필순,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

융비술시 실리콘 고무는 다루기 쉽고 조직 반응이 적어 현대 많이 돌출의 합병증이 생길 수 있다. 저자등은 이러한 실리콘의 단점을 보완할 수 있는 goretex를 사용하여 융비술을 시행하고 비첨에 대해서 자가연골을 이식하여 만족할 만한 결과를 얻었기에 goretex가 안면부 연조직의 증강을 위한 이식재료로 적합하다고 생각되어 문헌고찰과 함께 보고하는 바이다. Many different materials have been used to provide augmentation of the nasal dorsum. this include both autogenous and homogenous materials,as well as allografts. Silicone among the artificial materials has been most widely used. The silicone has some advantages and disadvantages. Goretex(expanded polytetra-fluoroethylene) which has widely used in vascular surgery and abdominal surgery can be used for facial soft tissue augmentation. The author presemts 2 cases of nasal augmentaion using goretex with literature review.