Pollen metabarcoding reveals the floral resources and niche overlap of honeybees in forest areas

Do Yuno,Park Woong‐Bae,Park Jun‐Kyu,Kim Chang‐Jun,Kim Il‐Kwon,Choi Moon Bo 한국곤충학회 2022 Entomological Research Vol.52 No.12

We investigated the floral resources of the honeybee (Apis mellifera)bypollenDNA metabarcoding as the first step in honeybee conservation. We attempted to identify as many floral resources and as wide resource availability as possible in areas and compared the richness of floral resources between forests and national parks. We found that both forest and national parks can sufficiently support honeybee floral resources; however, national parks were more stable habitats with more complex vegetation structure than those of forests. Also, honeybee’s preferences for floral resources were determined using niche breadth, niche overlap, and network analysis among the floral resources in forest areas. Quercus and Actinidia were the most frequently detected taxa of pollen resources in both forests and national parks by pollen metabarcoding. Even though we anticipated that more various pollen resource taxa would be found in national parks than forests, no differences in the number pollen resource taxa were detected between them. According to the niche overlap analysis result, the honeybee preferred the pollen resources in both forests and national parks, and the co-occurrence pattern analysis of pollen resource taxa in both forests and national parks showed a simple network. Altogether, our findings suggested that DNA metabarcoding of pollen collected by the honeybee can provide useful information on the flowering plants in a given area. As the use of floral pollen resources varies spatially and temporally, periodic monitoring of pollen resources can identify which pollen is required to maintain the health of honeybee colonies.

GG-38 : Minimal invasive surgery (LESS) for huge cystic adnexal tumors in whole abdomen

( In Young Choi ),( Ji Hye Lee ),( Mi Hyun Park ),( Ji Kwoun Park ),( Jeong Kyu Shin1 ),( Won Jun Choi1 ),( Soon Ae Lee ),( Jong Hak Lee ),( Won Young Paik ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

Laparoendoscopic single-site surgery (LESS) is helpful if not an evidence of suspicious malignancy in huge adnexa tumors. This approach might be reasonable alternative to conventional laparoscopic surgery. We report two cases with LESS for huge cystic adnexal tumors in whole abdomen. A 58 years old multigravida woman in menopause underwent removal of a huge cystic adnexal mass that occupied her entire abdomen by LESS.(ca.5200ml aspiration) A 15 years old single nulligravida woman had a huge cystic adnexal tumor in whole abdomen reaching to the level of the xyphoid process. A needle through the transumbilical single port was inserted directly under the mass. Subsequently, 8000 ml of cystic fluid was aspirated without spillage.

Development of a Reduction Algorithm of GEO Satellite Optical Observation Data for Optical Wide Field Patrol (OWL)

Sun-youp Park,Jin Choi1,Jung Hyun Jo,Ju Young Son,Yung-Sik Park,Hong-Suh Yim,Hong-Suh Yim,Hong-Kyu Moon,Young-Ho Bae,Jang-Hyun Park 한국우주과학회 2015 Journal of Astronomy and Space Sciences Vol.32 No.3

An algorithm to automatically extract coordinate and time information from optical observation data of geostationary orbit satellites (GEO satellites) or geosynchronous orbit satellites (GOS satellites) is developed. The optical wide-field patrol system is capable of automatic observation using a pre-arranged schedule. Therefore, if this type of automatic analysis algorithm is available, daily unmanned monitoring of GEO satellites can be possible. For data acquisition for development, the COMS1 satellite was observed with 1-s exposure time and 1-m interval. The images were grouped and processed in terms of “action”, and each action was composed of six or nine successive images. First, a reference image with the best quality in one action was selected. Next, the rest of the images in the action were geometrically transformed to fit in the horizontal coordinate system (expressed in azimuthal angle and elevation) of the reference image. Then, these images were median-combined to retain only the possible non-moving GEO candidates. By reverting the coordinate transformation of the positions of these GEO satellite candidates, the final coordinates could be calculated.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

초기 성공적인 심폐소생술후 혈청 Interleukin-6 농도의 발현양상

박규남,박승현,황두영,김형국,이환,최경호,이원재,정욱성,김학주,김세경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Objective : To investgate patterns of serial serum interleukin-6(IL-6) concentrations after initial successful cardiopulmonary resuscitation, and to access differences in serum IL-6 concentrations in patients with Cerebral Performance Category(CPC, 1-2) and CPC(3-5) at the 5th day after cardiacpulmonary resuscitation(CPR). Methods : We studied prospectively 12 patients with spontaneous circulation after cardiopulmonary resuscitation. Venous blood samples were taken 2, 4, 12, 24, 48 and 72 hours after restoration of spontaneous circulation to measure serum IL-6 levels by the enzyme-linked immunosorbent assay. Results : Serum IL-6 concentrations in the severe neurolgically disabled group(CPC, 3-5) were significantly increased at 2, 24 and 48 hours after restoration of spontaneous circulation compared with the less neurolgically disabled group. Also, serum IL-6 concentrations in the severe neurolgic disabled group showed dual peak at 4 and 24 hours. Conclusion : Our study demonstrates significant increase of serum IL-6 in patients with CPC(3-5) after successful cardiopulmonary resuscitation and the significant correlation between early increase of serum IL-6 and poor neurologic outcome. It suggests that early increase of serum IL-6 can be used as a prognostic marker after successful cardiopulmonary resuscitation.

다양한 표피 종양에서 Matrix Metalloproteinase-2와 Matrix Metalloproteinase-9 발현에 관한 면역조직화학적 연구

박홍표,최규철,정병수 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1

Background and Objectives: Degradation of basement membranes and extracelluar matrix is a essential step in tumor cell migration, invasion and metastasis formation. Two types of Matrix metalloproteinases (MMPs), MMP-2 and MMP-9 are active in the digestion of type Ⅳ collagen, the main constituent of the basement membrane. Based on this background, we investigated the expression patterns of Type Ⅳ collagenase (MMP-2 and MMP-9) in squamous cell carcinoma, adenoid (acantholytic) squamous cell carcimoma, and its precancerous conditions, actinic keratosis and Bowen's disease by immunohistochemical technique. Materials and Methods: Formalin-fixed and paraffin-embedded tissues from 5 actinic keratosis, 5 Bowen's diseases, 5 adenoid squamous cell carcinoma, and 5 squamous cell carcinoma were immunolabelled with monoclonal antibodies directed against MMP-2 and MMP-9. Result: For MMP-2, actinic keratosis, Bowen's disease, adenoid squamous cell carcinoma, and squamous cell carcinoma was expressed to relative low level in tumor cells. Bowen's disease showed a negative staining for MMP-9, but in microinvasive area of actinic keratosis and adenoid lining cells of adenoid squamous cell carcinoma was a positive staining or MMP-9. In Bowen's disease, squamous cell carcinoma, and adenoid squamous cell carcinoma, dyskeratotic cells and acantholytic cells were stained for MMP-2 but for MMP-9, only in adenoid squamous cell carcinoma. Conclusion: This finding suggested that MMP-9 may be related to tumor invasion to surrounding stroma and that MMP-2 and MMP-9 may be involves in the apoptotic process of the tumor cells.

E.D.M의 가공특성과 가공변질층에 관한 연구

崔圭夏,朴達根 忠州大學校 1999 한국교통대학교 논문집 Vol.34 No.1

In this paper the study of wire-cut Electric Discharge Machining on high speed steel, the characteristics such as cutting speed and evaluated for discharge voltage, discharge current, water speed. Cutting speed are improved as discharge current, cutting speed and discharge low voltage show a few increased, many increased discharge high voltage. Cutting speeds are many increased water low speeds, a few increased water high speed. Damaged layer a few decreased discharge high voltage, Damaged layer a few decreased high discharge current. Damaged layer are little by little decreased high water speed, many increased low water speed.

신사구체 경변증을 나타내는 FGS/NgaKist mice 간장에서 사이토크롬 P4501A1/2 단백질 발현

정규식,현병화,이철호,박상준,최양규,류시윤,이차수,민병길 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.2

아파트 내부공간에 대한 거주 후 평가 : 주공아파트 17평형을 대상으로 The 17 Pyung Size Housing Apartments Built in Korean National Housing Corporation

최보가,최무혁,박규정 경북대학교 1992 논문집 Vol.24 No.-

The purpose of this study is to make use of these materials for the next housing plan and to put emphasis on post occupancy evaluation. For this study, the apartments occupied are chosen and the degree of satisfaction with residence is examined, in real occupants' respect. As the objects, the apartments built by the Korean National Housing Corporation in 3 apartment sites of Taegu in 1979, 1983, 1985 is selected. The apartment size is restricted to 17 pyung. SPSS(Statistical Package for Social Sciences) is used as an analysis method and the degree of satisfaction is divided into 5 grade using Likert measurement to give objectivity. After the analysis, the degree of satisfaction with each apartment is compared and analyzed through Duncan's Multiple Test. The results are as follows. 1. In the satisfaction with each room, the degree of bathroom, kichen and dining room, balcony, entrance showed meaning difference. 2. Many factors out of evaluation factors showed meaning difference partly of room 1, room 2, room 3, kichen and dining room, bathroom utility room and boiler room, balcony in the degree of satisfaction with each apartment. 3. The improved factors, the factors which need to improve, the factors to which the occupants adapted themselves among evaluation factors are classified on the basis of standard satisfaction.

흰쥐에서 심정지후 뇌의 허혈-재관류 손상과 Tumor Necrosis Factor-α의 상관관계

최승필,박규남,박승현,박상현,정시경,김세경 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : Tumor necrosis factor-α(TNF-α) has been thought to play a major role in neurological injury during global brain ischemia and subsequent reperfusion following resuscitation in cardiac arrest. So, we hypothesized that the elevation in TNF-α was dependent upon the duration of the global brain ischemia, and related to delayed neuronal damage. Methods : Fourteen rats were divided two groups ; 1 minute-cardiac arrest group(n=7) and 3 minute-cardiac arrest group(n=7). we induced cardiac arrest by chest compression and clamping of tracheal tube for 1 minute and 3 minutes respectively. And then, resuscitation was initiated. To measure the plasma activity of TNF-α, blood samples were drawn before and at the end of cardiac arrest, and 30, 60, 90, and 120 minutes after initiation reperfusion. At 72 hours after resuscitation, the ND(neurologic deficit) score was determined and the histopathologic outcome of hippocampal CA1 neuron was observed by the percent dead hippocampal CA1 neurons. Results : 1. TNF-α level during the early reperfusion period(<2h) was significantly increased in 3 min-cardiac arrest group compared with 1 min-cardiac arrest group(p=0.0001). 2. There was a no significant difference of neurologic deficit score between 1 min-and 3 min-cardiac arrest. 3. Percent dead hippocampal neurons were significantly increased in 3 min-cardiac arrest group compared with 1 min-cardiac arrest group(9.1±1.2% vs 1.2±0.9%, p<0.05). Conclusions : The results suggest that longer duration of global brain ischemia causes a more profound increase in plasma TNF-α level during the early reperfusion period(<2h) and more delayed neuronal damage than lessor duration of global brain ischemia, and that increase in TNF-α level during the early reperfusion period(<2h) is related to delayed neuronal damage.