複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

수종의 광독성 약물투여 후 시간에 따른 피부농도 및 최소 광독량에 관한 연구

박윤기,조무연,한승경,임성빈 ( Yoon Kee Park,Moo Yon Cho,Seung Kyung Hann,Sung Bin Im ) 대한피부과학회 1991 大韓皮膚科學會誌 Vol.29 No.5

N/A The Skin Concentration and Minimal Phototoxic Dose Following Administration of Phototoxic Drugs as a Function of Time Yoon-Kee Park, M.D., Moo Yon Cho, M.D., Saung Kyung Hann, M.D., Sungbin im, M.D. Department of Dermatology Yonsei Univeraity College of Medicine, Seoul, Korea Psoralen photochemotherapy(PUVA) is defined as a treatment that depends on the interaction of a photosensitizing substance, psoralen, and long wave jltraviolet(UVA) radiation that results in therapeutically beneficial effects. The rationale of its use is to clear skin disease by repeated controlled phototoxic reactions. The efficacy of PUVA depends on the drug, the UVA dose and wave length, the individual sensitivity to the phototoxic reaction and on the disease to be treated. However, the major factor is the amount of psoralen at the site of photochemical reaction in skin. Daspite the extensive experiences which have now accumulated in the use of PUVA, the relationship between the therapeutic response and phototoxicity as it occurs in normal skin is not established. Comparative data on different psoralen molecules and their skin sensitizing ability are required. From studies on the skin concentration using HPLC and minimal phototoxic dose following oral administration, intraperitoneal administration, a bath of phototoxic drugs as a function of time in guinea pigs, the following results are obtained. 1. The skin concentration of phototoxic drugs after oral administration peaked at 1.5 hours, and the concentration of 8-methoxypsoralen(8-MOP) was 3.7 times greater than that of 5-methoxypsoralen(5-MOP). The skin concetration of 4,5,8-trimethylpsoralen(TMP) was not detected in this study(limit of sensitivity 5ng/g). 2. The skin conentration of phototoxic drugs after intraperitoneal administration peaked at 30 minutes, and the concentration of 8-MOP was slightly greater than that of 5-MOP. The skin concentration of TMP was not detected in this study(limit of sensitivity 5ng/g). 3. The skin concentration of phototoxic drugs after bathing peaked immediately after bathing, and the skin concentrations of phototoxic drugs after bathing decreased in the order of 5-MOP, TMP, and 8-MOP. 4. The minimal phototoxic dose of phototoxic drugs after oral administration was lowest at 1.5 hours, and the minimal phototoxic dose of 8-MOP was 8 times less than 5-MOP, 24 times less than TMP. The phototoxicity of 8-Mop after oral administration was strongest. The time of lowest phototoxic dose and highest skin concentration was the same. 5. The minimal phototoxic dose of phototoxic drugs after intraperitoneal administration was lowest at 30 minutes, and the minimal phototoxic dose of 8-MOP was 1.3 times less than 5-MOP, 5.7 times less than TMP. The phototoxicity of 8-MOP after intraperitoneal administration was strongest. The time of lowest phototoxic dose and highest skin concentration was same. 6. The minimal phototoxic dose of phototoxic drugs after bathing was lowest at 15 minutes. The minimal phototoxic dose of 8-MOP was not significantly different but was significantly greater than that of 5-MOP.

C3H 생쥐에서 자외선 B의 집중조사와 분할조사가 표피내 랑게르한스 세포의 회복에 미치는 영향

구상완,한승경,박윤기,조무연 ( Sang Wahn Koo,Seung Kyung Hann,Yoon Kee Park,Moo Yon Cho ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.4

N/A The Effect on the Recovery of Epidermal Langerhans Cells in C3H Mice after Single and Fractionated Exposure of Ultraviolet B Irradiation Sang-Wahn Koo, M.D., Seung Kyung Hann, M.D., Yoon-Kee Park, M.D., Moo Yon Cho, M.D. Department of Dermatology, Yonsei University College of Medicine Seoul, Korea The study was undertaken to see the recovery in epidermal Langerhans cells in relation to time after UVB irradiation through different amounts and ways of exposure in C3H mice. We irradiated the ears of C3H mice with UVB 200mJ/㎤ and 400mJ/㎤ in a single dose at one time and 5 fractionated doses for 5 days and 10 fractionated doses for 10 days and performed biopsies on the ears of the control and experimental groups after, 2, 7, 14, 21 days of irradiation and stained them by the immunoperoxidase method. The results are summarized as follows. 1. A more significant decrease in the number of Ia-positive epidermal Langerhans cells was observed in single-dose-exposed group than fractionated-dose-exposed groups on the second day of irradiation. 2. On the seventh day of exposure, the number of the Ia-positive epidermal cells in single-dose-exposed group returned to normal, but in fractionated-dose-exposed groups the number of Langerhans cells decreased most remarkably. 3. In fractionated-dose-exposed groups, the number of Langerhans cells returned to normal on the 14th or 21st day of expposure. (Kor J Dermatol 29(4) : 459-465, 1991)

운동시의 습관적인 이 악물기가 치경부 수복물에 가해지는 스트레스를 분산하기 위한 마우스가드의 사용

윤성영,송창규,박세희,김진우,조경모 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.1

마우스가드는 19세기 복싱선수들의 구강내 열상과 연조직의 외상을 막기 위해 사용되었다. 최근에는 치과영역에서 다양하게 사용되고 있으며, 운동 보호 장치, 미백용 장치물, 교정용 유지 장치, 수술용 가이드 장치, 턱관절 안정 장치물 등에 쓰이고 있다. 습관적인 이 악물기에 의한 반복적인 5급 수복물의 탈락은 우선 이 악물기에 의한 스트레스를 조절한 후 수복이 이루어져야 한다. 마우스가드는 이 악물기에 의한 스트레스를 조절하는 장치물로 사용할 수 있다. 본 증례에서는 마우스가드로 치아에 가해지는 스트레스는 감소시키는 것에 대해 논의 할 것이다. 마우스가드를 사용함으로써 반복적인 탈락을 나타내는 5급 수복물의 유지에 단기적으로 만족스러운 결과를 얻을 수 있었다. 만약 환자 및 치과의사가 이 악물기로 반복적인 탈락을 나타내는 수복물로 고생하고 있다면, 마우스가드의 보조적인 사용이 치경부 수복물의 유지에 도움을 줄 수 있을 것이다. Mouthguards were used to protect boxers from lip lacerations and other soft tissue injuries in the late 19th century. Now they are used various parts of dental treatment, which are sports protective aid, bleaching tray, orthodontic retainer, implant insertion guide tray, splint and so on. Repeated dislodgement of Class V restoration due to habitual clenching stress should be restored with stress control. Mouthguard can be used as stress relief device. This case describes methods that can relieve occlusal force to teeth by using mouthguard. Satisfactory results can be obtained by using mouthguard for retention of repeated dislodgement Class V restorations. If patients suffered from repeated restorations of Class V due to clenching, mouthguard can be used additional device to relieve the occlusal stress in conservative dentistry.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

상아질 표면 젖음성이 수종 자가접착레진시멘트의 인장접착강도에 미치는 영향

윤성영,박세희,김진우,조경모 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.2

본 연구의 목적은 다른 표면 젖음성을 갖는 상아질에 대한 수종의 자가접착레진시멘트의 인장접착강도를 비교하고자 하는 것이다. 이번 실험을 위해 Rely-X Unicem (3M. ESPE. St. Paul. MN. USA). Embrace Wetbond (Pulpdent. Oakland. MA. USA). Maxcem (Kerr. Orangc. CA. USA) 3종의 자가접착레진시멘트를 사용하였다. 발거한 상, 하악 대구치 60 개를 미세절단기 (Acutom P-50, Struers, Copenhagen Denmark)를 이용해 한 치아에서 2 mm × 2 mm × 7 mm의 치아 시편 두개씩 얻었다. 상아질 표면을 건조 그룹은 10 초간 강한 공기로 건조시켰고, 습윤 그룹은 과잉의 수분을 2 초간 거즈로 압박해 제거하였다. 상아질 표면의 젖음성을 달리한 시편에 자가접착레진시멘트를 접착한 후 상온의 상대습도 100% 상태에서 24 시간 보관했고, 만능시험기 (EZ Test, Shimadzu corporation, Kyoto, Japan)를 이용해 인장접착강도를 측정했고, 분리된 접착면의 파절양상을 근관 치료용 현미경 (OPMI pro. Carl Zeiss. Oberkochen. Germany)을 이용해 20 배의 배율로 관찰하였다. SPSS™ Ver 10.0 (SPSS Inc.. Chicago. IL. USA)을 사용응하여 동일한 자가접착레진시멘트에서 상아질 젖음성에 따른 인장접착강도를 T-test로 비교하였으며, 각 자가접착레진시멘트간의 인장접착강도를 One way ANOVA test로 비교 분석하고 95% 유의수준에서 Scheffe's test로 사후검정 하였다. 실험 결과 자가접착레진시멘트의 인장접착강도는 상마질 젖음성에 영향을 받지 않았으며, 상아질 표면 젖음성과 상관없이 Maxcem의 인장접착강도가 Unicem과 Embrace의 인장접착강도보다 유의성 있는 낮은 값을 나타내었다. 또한 모든 시편의 분리된 접착면은 접착실패 양상을 나타내었다. 이상의 실험 결과로 볼 때 자가접착레진시멘트는 상아질 표면 젖음성에 상관없이 사용할 수 있을 것이라고 생각된다. The purpose of this study was to compare the tensile bond strength of several self-adhesive resin cements bonded to dentin surfaces with different wet conditions. Three self-adhesive resin cements:Rely-X Unicem (3M ESPE. St. Paul, MN USA). Embrace Wetbond (Pulpdent. Oakland. MA, USA). Maxcem (Kerr. Orange, CA USA) were used. Extracted sixty human molars were used. Each self-adhesive resin cement was adhered to the dentin specimens (two rectangular sticks from each molar) in different wet conditions. Tensile bond strength were measured using universal testing machine (EZ Test Shimadzu corporation Kyoto. Japan) at a crosshead speed of 1.0mm/min. After the testing bonding failures of specimens were observed by Operative microscope (OPMI pro. Carl Zeiss, Oberkochen, Germany). T-test was used to evaluate the effect of dentin surface wetness. One-way ANOVA test was used to evaluate the tensile bond strength of self-adhesive resin cements in the same condition. Scheffe's test was used for statistical analyzing at the 95% level of confidence. The result showed that wetness of dentin surface didn't affect tensile bond strength of self0adhesive resin cements and Maxcem showed the lowest tensile bond strength.

Clostridium difficile 장염에 의한 속발성 유전분증에서 발현된 독성 장염

조형원,정혜경,강현주,이윤표,강혜원,심기남,정성애 Ewha Womans University School of Medicine 2014 EMJ (Ewha medical journal) Vol.37 No.1

Amyloidosis is characterized by extracellular deposition of protein fibrils in one or multiple organs. AA amyloidosis is secondarily occurred to be related with chronic infections or inflammatory diseases. We report a 67-year-old man suffered from secondary AA amyloidosis related with chronic Clostridium difficile colitis after repeated total hip replacement surgery. Infection control is the most important treatment of AA amyloidosis secondary to chronic infection. However, the patient’s C. difficile colitis was not controlled well, eventually toxic megacolon with sepsis was developed. Consequently, he had to take total colectomy, but he expired with multi-organ failures. We suggested that early surgical procedure might be one option for intractable C. difficile colitis complicated with secondary amyloidosis.

한국인의 정당관에 대한 실험적 고찰

조일문,윤경우 건국대학교 1970 學術誌 Vol.11 No.1

This article is prepared to serve the purpose of experimentally analyzing the nature and changes of Korean attitude toward political party in recent years. It is based on a sample study about political parties in Korea and analyses of relevant literatures and materials. The sample study had been conducted in a most selective way in view of the limited number of personnel, expenses and the pressure of time. In order to eliminate any bias or prejudice on the part of interviewers, however, special efforts had been made in selecting samples adequate enough to represent the sex, occupation, age, educational background and the compositive ratio among the population. The results of sample study on the basis of 1,002 respondents might be summarized as follows : 1. The necessity of political party is particularly emphasized by male population, urban residents, younger generation, intellectuals and propertied classes. Though in small number altogether, those who think political party unnecessary are found in a relatively greater number in urban areas than in rural areas. 2. There are now many political parties registered in Korea. The results of the survey about what group regards political system composed of how many political parties as most desirable are as follows ; two or three parties‥‥‥‥Supported by females, rural dwellers, the aged, the lower-educated. three or four parties‥‥‥‥Supported by males, urban residents and intellectuals. In general, the degree of recognizing the value of political parties is considerably high with regard to the two major parties, especially with regard to the government party. High degree of recognition for government party is attributable to the fact that the ruling party is in a position to penetrate deeply into the masses with the support of the government. Those who are more familiar with political party tend to insist upon the greater necessity of political party. But this has no direct bearing with the ruling that only party nominees can be candidates for the Presidency and the National Assembly membership, and that independents without party affiliation are prohibited from running as candidate. 3. In selecting Presidential candidates and National Assembly candidates, more emphasis is usually placed upon their personality and public pledges than upon their party affiliation. Respondents who synthetically considered the following three elements-political party, public pledges and personality-amounted to 36.7%. Among them, about 70% considered primarily in terms of personality. The degree in which continuous support to the same party is very low and this tendency seems to have something to do with Korean tradition of taking into serious consideration the personality itself rather than political party. 4. Half of the respondents are in favor of the measure that independents without party affiliation and consequently without party recommendation may become candidates for the member of National Assembly, while one-thirds of them are againt it. They think independents should be given opportunities to run as candidates for National Assemblymen. Though they admit the very necessity of political parties, they still seem to be of the opinion that corruptions accompanying party politics have to be dispensed with. 5. Arguments in favor of plural party system are that it is the very foundation of democracy and performs the function of integrating and coordinating the general will of the people. In particular, party members among the respondents favor the existence of political parties and oppose independent candidates running in the National Assembly election. 6. The most important reason why some of the respondents are against the existence of multiple political parties is that their activities accelerate political corruptions and sacrifice national interests through partisan causes. 7. Whether people support any particular political party or oppose it depend more upon the leading figures of the party than the degree of social representation, policy or accomplishments of that party. The dominant tendency to rely upon personality in selecting public candidates reveals the backward attitude of the Korean people towards political party. 8. On the question of adequate number of political party required, half of the interviewers support the bipartisan system and one-thirds the three-party system. Members of the New Democratic Party are overwhelmingly in favor of the two-party system , while members of minor parties are unanimously in support of multi-party system. 9. Most respondents think that only if a man filed an application he is fully qualified to be a party member and that political party is more concerned with formalities than with the exercise of rights or fulfilment of duties on the part of party members. 10. Many respondents consider it to be legitimate to raise political fund through the party members' contribution or from the sources of national treasury so as to use it as a safeguard against political corruptions and injustices. Those respondents who possess high consciousness as party member emphatically argue in favor of the need of party members' contribution. However, it reflects their view only and it is doubtful whether this argument could be realized. 11 Many respondents, more among party members than among nonparty members, expressed opinions in a negative way about intra-party democratic practices. Though many of them favor in principle the democratic order among political parties, they anticipate that it is hardly feasible because it is completely left to the voluntary ruling in each party's constitution. More party members than non-party members, and more intellectuals than illiterates support the possibility of democratizing intra-party order. 12. Looking into the changes in Korean attitude toward political party in the past and present, one can learn that gradual changes have taken place ranging from complete distrust of it in the immediate aftermath of the Liberation to a relatively general trust in it in the later stage. The present tendency suggests us that there will be a possibility of significant transition into a total trust in the political party system among Korean people in the future. Nonetheless, there is also a tendency for many people to discredit party members and policies, event though there are a few who trust political party. The expectation for political party does not seem to be improved. For the future of party politics, many people bear an opinion that supra-party movement is likely to emerge in order to overcome the bad influence of plutocracy. Yet, many people still expect that party-centered vote and peaceful change of regime shall be eventually made possible despite the inactivity of National Assembly.

EGDA 기능성 모노마로 희석된 Epoxy Acrylate의 전자빔 경화 및 코팅

조병기,윤경용,Petrov, S.E.,이재민,이정희,한도흥 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

Electron beam curing of acrylated epoxy resin diluted with mono-functional monomer EGDA has been investigated under circumstances contacted with air and without air. The formulated resin in contact with N2 or covered with film could be cured up to 95% even with low dose of 5kGy and the surface of cured resin became very hard, but cured resin in existence of oxygen and without any additive had a low degree of curing and the surface became sticky due to insufficient curing. Additives such as curing agent P-115 or initiator BPO could increase the degree of curing to more or less one, but these additives could not remove completely surface stickiness. While, at high dose(more than 100kGy), addition of BPO were possible to make the irradiated surface of the resin be non-sticky even in existence of air. Degree of curing of the resin which contained pigment was very high at dose of around 20kGy. Elongation and stress at break on the cured resin with ferric oxide pigment were be higher than with non-ferric oxide pigment. Adhesion and anti-firing of the resin coated on cement block could be excellent.