In situ culture and harvest of aminocytes using covership processing method

Cheong, Kyung-Soon,Hwang, Do-Yeong,Lee, Dong-Sook,Cho, Soo-Kyung,Kim, Ki-Chul,Min, Eung-Gi Korean Society of Medical Genetics 1998 대한의학유전학회지 Vol.2 No.2

Genetic amniocenteses were performed in a series of 127 patients as a routine study. Samples from the patients were cultured by in situ method, flask method or both according to the state of amniotic fluid. The overall success rate of culture was 97.6% and no culture failure was observed in the flask method. It took 5 days first of all and 8.15 days average from set-up to harvest and there were 7.2 colonies per dish in in situ method. Therefore, it is suggested that in situ method which decreased the mean culture days and made clonal analyses possible, is a clinically available and even more reliable method in parallel with flask method in prenatal diagnosis.

개정된 예방적 항생제 투여에 따른 선천성 심장질환 환자의 치과치료

정미영,김종철 大韓小兒齒科學會 1991 大韓小兒齒科學會誌 Vol.18 No.2

Patient with congenital heart disease are susceptible to bacteral endocarditis, and bacteremia following dental procedures may lead to bacterial endocarditis in these patients. Therefore prophylactic antibiotics are recommended for patients with congenital heart disease who are undergoing dental procedures. In 1984,American Heart Association revised guidelines for a prophylaxis against bacterial endocarditis and these include a large loading dose of antibiotics with a single postoperative booster. For most patients, oral penicillin is recommended and for those allergic to penicillin, erythromycin is preferred antibiotic. This report presents two cases of dental treatment of patient with congenital heart disease under the most recent American Heart Association recommendations and for antibiotic prophylaxis.

알코올의존증 치료약물 복용 여부 모니터링을 위한 소변 중 크레아티닌 농도로 보정된 날트렉손 및 6β-날트렉솔 측정값 평가

심영은(Yeong Eun Sim),김선영(Seon Yeong Kim),정재철(Jae Chul Cheong),이현수(Hyun Soo Lee),표재성(Jaesung Pyo),김진영(Jin Young Kim) 대한약학회 2022 약학회지 Vol.66 No.2

This study aimed to detect naltrexone (NTX) and its metabolite 6β-naltrexol (6βNTX) in the urine samples of parolees or probationers who were legally bound to participate in an alcohol dependence treatment program to determine whether to follow the medical treatment ordered by the court. The developed liquid chromatography-tandem mass spectrometry method was suitable for the simultaneous detection of NTX as well as its active metabolite 6βNTX in urine samples. The ratio of creatinine to the analyte ([analyte, ng/mL]/[creatinine, mg/dL]) was used to exclude inaccuracies caused by urine dilution, and this value was further corrected by the average urine creatinine concentration in Korean adults (male 132.6 mg/dL, female 93.3 mg/dL). The cut-off values for NTX and 6βNTX were set at 10 ng/mL. If both the parent drug and its metabolite were found in urine and the corrected creatinine concentrations of NTX and 6βNTX were more than 10 ng/mL, it was considered that the participants were following their treatment protocol accordingly. The results showed that 81 (94.2%, male 69 and female 12) of 86 participants were taking NTX, whereas five (four males, one female) were not adhering to the protocol accordingly. It was found that the four male participants (4.9%) of the 81 participants who were taking the drug initially had urinary NTX and 6βNTX concentrations below the cut-off level without correcting creatinine level, but the values met the acceptance criteria (i.e., >10 ng/mL) of the medical treatment program after correcting the creatinine level. The proposed method can be applied to other drugs in the future.

Determination of picogram-level zolpidem and its main metabolites in hair by LC-MS/MS and GC-MS/MS

Seon Yeong Kim,Nam Hee Kwon,Sung Ill Suh,Jae Chul Cheong,Jin Yong Kim 한국분석과학회 2022 학술대회논문집 Vol.2022 No.11

경주지역 택시운전사들의 생활습관과 비만과의 관련성

장철호,최연악,이화미,강윤성,김창희,노수영,임영빈,임우현,이관,임현술,정철,배근량,정해관 東國大學校醫學硏究所 2004 東國醫學 Vol.11 No.2

택시운전사들을 대상으로 직업적 특성과 생활습관에 대해 알아보고, 이러한 생활양식이 비만 특히 복부비만과 어떠한 관련성이 있는지 알아보고자 이 연구를 시행하였다. 본 연구는 경주지역에서 근무하는30세 이상의 남자 택시운전사 105명을 대상으로 시행하였다. 2003년 9월 20과 21일에 걸쳐 직접 면담을 통해 설문지를 작성하고, 신체를 계측하였다. 본 연구에 사용된 신체 지수에 관련된 용어는 『건강통계자료 수집 및 측정의 표준화』의 정의를 이용하였다. 신체계측은 체중, 체지방률, 허리둘레 및 엉덩이둘레를 각 1인이 담당하여 측정하였다. 조사대상자의 신체측정치의 평균은 신장 169.8±5.5 cm, 체중 73.2±8.8 Kg, 허리둘레 92.5±7.5 cm, 엉덩이둘레 99.9±6.5 cm, 체지방률 24.7±4.5%, 체질량지수 25.3±2.3 Kg/m², 허리엉덩이둘레비 0.93±0.04이었다. 조사대상자의 비만율은 체질량지수를 기준으로 25 이상이 56명(53.4%)이었고, 허리엉덩이둘레비를 기준으로 0.9 이상이 80명(76.2%)이었다. 허리둘레를 기준으로 91.3 이상이 58명(55.2%)이었고, 체지방률을 기준으로 25% 이상이 47명(44.8%)이었다. 허리둘레와 체질량지수의 상관계수는 0.74로 엉덩이둘레 0.66, 허리엉덩이둘레비 0.39, 체지방률 0.57보다 높았다. 로지스틱 회귀분석에서 적당한 규칙적인 운동을 하는 군이 거의 하지 않는 군에 비하여 교차비는 0.15 (95% CI: 0.04-0.54)이었다. 또한 규칙적인 식사를 하는 군에 비해 중증도로 규칙적인 식사를 하는 군의 교차비는 6.29 (95% CI: 2.28- 17.34)로 중증도 식사규칙성군에서 비만율이 높았다. 신체적, 경제적 측면에서 상대적으로 열악한 근무조건에 처해있는 택시운전사들에서 높은 비만율을 관찰하였다. 이런 높은 비만율은 교대근무와 장시간의 근무시간으로 인한 운동 부족과 불규칙한 식생활로 인해 유발되었을 가능성이 높았다. 비만의 위험성에 대한 택시 기사들의 인식을 환기시키는 대책이 필요할 것이며, 기업은 자체적으로 보건교육을 강화하고, 여가 활용을 장려하거나 사내 운동 시설의 설립 등의 제도적인 차원의 비만 대책을 강구할 필요가 있다고 생각한다. Obesity is closely related to the socioeconomic factors and life style. Cab drivers are exposed to unfavorable life style due to their long shift work. We studied the relationship between obesity and life style factors in selected cab drivers. We conducted a questionnaire survey and measured the body weight, waist and hip circumference of 105 cab drivers over 30 years old from Sep. 20 to 21, 2003 in Gyeongju. We developed Dongguk Index (DI) to categorize the diet patterns of cab drivers and defined abdominal obesity as a waist circumference ≥91.3 cm. The proportion of obesity in our study population was 53.4% by body mass index (BMI), 76.2% by waist-hip Circumference (WHC), 55.2% by waist circumference (WC), and 44.8% by body fat (%). WC was significantly higher in longer working (≥12 hours), high pack-years of smoking, less exercising, and less regularly eating group than in shorter working (<12 hours), low pack years of smoking, frequently exercising, and regularly eating group (P<0.05). Odds ratio of regular exercise for obesity was 0.15 (95% CI: 0.04-0.54) for moderate exercising group and 0.53 (95% CI: 0.17-1.65) for frequently exercising group. Odds ratio of regluar diet for obesity was 6.29 (95% CI 2.28017.34) for those with moderately regular diet and 3.09 (95% CI0.96-9.92) in those with poor regularity in diet. In cab drivers, long working hours, and 12 hours per shift together with their irregular diet and exercise patterns makes the matter worse. Because of high socioeconomic costs caused by obesity, obesity is not simple problems to individual, but problems in view of public health. Supports and concerns for prevention and control of obesity will be needed.

Simultaneous determination of 12 neuroleptic drugs and their metabolites in urine by using LC-MS/MS

Jeong Eun Kim,Seon Yeong Kim,Jae Chul Cheong,Jin Young Kim 한국분석과학회 2023 학술대회논문집 Vol.2023 No.11

Analysis of Trace Elements in Korean Pork by ICP-MS and Application Techniques for Geographic

Yu Min Park,Cheong Mi Lee,Eun Yeong Nho,Ji Yeon Choi,Jong-Hyun Jung,Young-Chul Jung,Kyong Su Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

미배양 융모막융모세포에서 FISH의 이용

장은주(Eun Ju Chang),황도영(Do Yeong Hwang),최혜숙(Hyeh Sook Choi),정경순(Kyung Soon Cheong),김기철(Ki Chul Kim),민웅기(Jin Choe),최진(Jin Choe),최영민(Young Min Choi),문신용(Shin Yong Moon) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.2

Objective: The cytogenetic analysis for earlier detection of fetal chromosome aneuploidies is performed from chorionic villus using either long-term culture or direct chromosome preparation. To analyze the cause of pregnancy loss, we also attempt the cytogenetic study in product of conception(POC) using chorionic villi or fetal tissue. But the failure of analysis often occurs in direct preparation of villus cells and product of conception(POC). We studied to evaluate the clinical usefulness of FISH in uncultured chorionic villus cells of culture-failed cases. Methods: According to the patient's indication, we performed FISH for chromosome 18, 21, X and Y in chorionic villi as well as POC and compared FISH results with their chromosomal studies. Results: We found one trisomy 18 and one trisomy 21 in Chorionic Villus Sampling and one trisomy 18 and one monosomy X(45, X) in POC. The averages for accuracy of FISH were 83-91% and all cases are represented consistent results with their chromosomal studies. Among them, we could analyze using FISH only in 5 cases of culture failure including one case of monosomy X in POC. Conclusion: We could detect aneuploidy with uncultured chorionic villus cells in case of culture failure, using FISH, it may be the potential method to assist the cytogenetic study.

수정된 설계 방정식을 이용한 허니컴 구조의 차폐 효과 예측

이경원,정영철,홍익표,육종관,Lee Kyung-Won,Cheong Yeong-Chul,Hong Ic-Pyo,Yook Jong-Gwan 한국전자파학회 2005 한국전자파학회논문지 Vol.16 No.9

In this paper, the modified design equation of shielding effectiveness was presented to predict more accurately the shielding effectiveness of honeycomb structure with hexagonal waveguide. The design equation of shielding effectiveness in honeycomb was represented from adding shielding effectiveness of single lattice to shielding effectiveness of infinite array of single lattice. This paper proposed the generalized design equation of shielding effectiveness by analyzing basis lattice of hexagonal waveguide which composes honeycomb structure and infinite array structure of basis lattice. To provide the validity of the modified design equation of shielding effectiveness in this paper, comparison with other available date using 3D EM commercial software is made.

Clinical Implications of Microsatellite Instability in Early Gastric Cancer

Kim, Dong Gyu,An, Ji Yeong,Kim, Hyunki,Shin, Su-Jin,Choi, Seohee,Seo, Won Jun,Roh, Chul Kyu,Cho, Minah,Son, Taeil,Kim, Hyoung-Il,Cheong, Jae-Ho,Hyung, Woo Jin,Noh, Sung Hoon,Choi, Yoon Young The Korean Gastric Cancer Association 2019 Journal of gastric cancer Vol.19 No.4

Purpose: We aimed to evaluate the clinical characteristics of microsatellite instability in early gastric cancer. Materials and Methods: The microsatellite instability status of resected early gastric tumors was evaluated using two mononucleotide repeat markers (BAT25 and BAT26) and three dinucleotide repeat markers (D5S346, D2S123, and D17S250). Tumors with instability in two or more markers were defined as microsatellite instability-high (MSI-H) and others were classified as microsatellite stable (MSS). Results: Overall, 1,156 tumors were included in the analysis, with 85 (7.4%) classified as MSI-H compared with MSS tumors. For MSI-H tumors, there was a significant correlation with the female sex, older age, tumor location in the lower gastric body, intestinal histology, lymphovascular invasion (LVI), and submucosal invasion (P<0.05). There was also a trend toward an association with lymph node (LN) metastasis (P=0.056). In mucosal gastric cancer, there was no significant difference in MSI status in tumors with LN metastasis or tumors with LVI. In submucosal gastric cancer, LVI was more frequently observed in MSI-H than in MSS tumors (38.9% vs. 25.0%, P=0.027), but there was no difference in the presence of LN metastases. The prognosis of MSI-H tumors was similar to that of MSS tumors (log-rank test, P=0.797, the hazard ratio for MSI-H was adjusted by age, sex, pT stage, and the number of metastatic LNs, 0.932; 95% confidence interval, 0.423-2.054; P=0.861). Conclusions: MSI status was not useful in predicting prognosis in early gastric cancer. However, the frequent presence of LVI in early MSI-H gastric cancer may help guide the appropriate treatment for patients, such as endoscopic treatment or limited LN surgical dissection.