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흰쥐 부정소 상피세포, 내강액 및 성숙 전후 정자에서의 생리화학적 변화
정경순,박용빈,최임순 한국통합생물학회 1991 동물학회지 Vol.34 No.2
흰쥐 부정소 정자의 성숙 전후에 일어나는 변화를 몇가지 효소를 중심으로 관찰하였고 그 성숙과정중에 일어나는 상피세포, 내강 및 정자 사이의 상호관계를 알아보기 위하여 실험군 별로 몇가지 효소의 활성도와 탄수화물 잔기의 함량을 측정하였으며, 전기영동을 이용하여 각 군의 차이를 관찰하고 이에 대한 웅성호르몬의 관련성을 알아보았다. 1. 부정소 두 정자와 부정소미 정자에서 활성도 측정시 lactate dehydrogenase, glucose-6-phosphatase 및 Na+ -K+ -ATPase의 경우는 유의한 차이가 없었으며, $Mg^2$+-ATPase의 경우만이 부정소미 정자가 부정소두 정자보다 유의성 있게 높은 활성도를 가지는 것으로 나타났다. 또한 부정소두와 부정소미 상피세포 , 내강 및 정자의 세군으로 나누어 각각 탄수화물 잔기를 정량하였을 때 hexosamine은 상피세포에서, sialic acid는 상피세포와 내강액에서 부정소미의 경우가 더 높은 함량이 존재하였으며, 내강액과 정자의 crude membrance fraction을 SDS-PAGE 했을 때 분자량이 33-37 KD 사이에 존재하던 band가 부정소미 내강액과 부정소미 정자의 crude membrance fraction에서 관찰되었으므로 흰쥐에서 정자의 성숙과정과 관련된 부정소내의 여러 변화를 비교하는 자료가 될 수 있었다. 2. 부정소 내강액에서 $\beta$ -glucuronidase와 $\beta$ -glucosidase의 활성도 및 웅성호르몬에 대한 의존성을 측정하였을 때 거세 후 5일째부터 이 두 효소의 활성도가 모두 유의하게 감소하기 시작하였고, tentosterone을 투여하였을 때는 $\beta$ -glucuronidase는 투여 5일, $\beta$ -glucosidase는 투여 10일 후부터 유의하게 증가하였으며 웅성호르몬에 대한 내강액의 의존성을 알아보기 위하여 SDS-PAGE하였을 때 tentosterone투여군의 부정소두에서 분자량이 약 21 KD에 해당하는 band를 새로이 관찰하였다. It has been investigated what could be the selective marker distinguishing the immature from mature spermatozoa and whether fi -glucuronidase and fi -glucosidase are dependent on androgen in the luminal fluid of the epididymis or not. The contents of hexose, hexosamine and sialic acid in the epithelial cells, luminal fluid and spermatozoa of the epididymis were examined and the patterns of protein bands were compared in each group of the luminal fluid by SDS-PAGE. Lactate dehydrogenase, glucose-6-phosphatase, Na+ -K+ -ATPase and MgNa-ATPase showed higher activities in the cauda than the caput epididymal spermatozoa but only $Mg^2$+-ATPase activity appeared to be changed significantly. When the contents of hexose, hexosamine and sialic acid were analyzed and compared quantitatively, those of hexose were significantly different in the luminal fluid of caput and cauda epididymis, those of hexosamine in the epithelial cells and those of sialic acid in the epithelial cells and luminal fluid. When SDS-PAGE has been performed in each group, the band of MW 33-37 KD which was absent in the luminal fluid of caput epididymis appeared obviously in the luminal fluid of cauda epididymis and ako apeared in the cauda sperm crude membrane fraction. In addition, $\beta$ -glucuronidase and $\beta$ -glucosidase activities and their dependence on androgen were measured and the SDS-PAGE patiems of proteins and/or glycoproteins in the luminal fluid were examined. The activities of these two enzymes in the luminal fluid of the epididymis decreased significantly from the 5th day after castration. When testosterone was injected, the activity of $\beta$ -glucuronidase began to increase significantly from the 5th day following injection and that of $\beta$ -glucosidase from the loth day. On the other hand, the band of about MW 21 KD was newly observed in the lumen of caput epididymis when testosterone was administered.
정경순 김천과학대학 2006 김천과학대학 논문집 Vol.32 No.-
This study was done to analyze research trends and to suggest future directions for nursing research on the quality of life(QL) of chronic illness. This article reviewed 32 nursing researches on QL between 1995 and 2000, by examining them according to the period of publication, research design, type of subjects, instruments of measurement and range of reliabilities, type of nursing intervention, and association of QL and related variables. The findings of the analysis can be summarized as follows 1. The most of studies (23articles) identified surveys and correlational studies 2. The simular concepts were 4 types , including QL(20 articles), quality satisfaction, life satisfaction, and well-being. Among them, QL was the most often used. 3. Research subjects were classified into illness people(24 articles), healthy person(4articles), family caregivers (4 articles) in sequence. The majority of the patients were cancer patients(7articles). 4. The instruments of measurement used for data collection were 14 types. Among them, the QL Scale by You-Ja Ro(1988) was the most widely used in the studies(16 articles). The reliabilities of the QL Scale were reported in 29 articles, and the Cronbach's alpha coefficients were over 0.8. 5. The most distribution of related concepts in survey and correlational studies were economic status, family support, perceived health status, self-esteem. Among them, economic status were main related concept. 6. The types of intervention used for experimental studies(6) were categorized as exercise(3), education(2), social supportive meeting(l). On the basis of the above findings, the following recommendations were made 1. The development of comprehensive tool, including physiologic, affective, psychosocial, spiritural aspect is needed. 2. The revitalization of experimental studies is needed through various nursing interventions application for improvement of QL 3. A Meta-analysis needs to be conducted to identify intervention effects on the quality of life. 4. To use precisional concept on QI, there is needed to identify of concept through qalitative research 5. For further studies on QI are needed the comprehensive analysis, including non-degree and degree dissertation.
정경순,노명희,김옥란 김천과학대학 2004 김천과학대학 논문집 Vol.30 No.-
This study was conducted to search for characteristics of unwed mother. The subjects were residing 160 unwed mothers in welfare facilities located in Seoul, Gyeonggi, Jeonnam and Daegu. The data were collected from August 2002 to February 2003. The major findings were as follows. ·The subjects were under 20 years(50.6%), under high school graduate(52.2%), having both parents(60%). ·Among the subjects, 74.4% were to choose the adoption, 83.3% were the time of first sexual relation under 15years, 55% were not of former pregnancy experience, 50% had experience of abortion in former pregnancy. ·First sexual relation was acted natually(50%) but after sexual relation, 49.4% was feeling of repent ·Attitude of sexual relation was represented outstandingly that sexual relation may be a consider expression of love. ·Among the subjects, the reason of noncompliance of contraception were because of unknown to method of contraceptions(21.9%). We need to develop educational programe to improve compliance and knowledge of contraception of unwed mother.
정경순 김천과학대학 2011 김천과학대학 논문집 Vol.37 No.-
The purpose of this study is to identified trend of studies and to analyzed the research related to unmarried single childrearing mothers. Recently, Unmarried single childrearing mothers are increasing inspite of burden on childrearing. They live with economic and socio-psychologic difficulty. The qualitative studies on unmarried single childrearing mothers were on process of life , process of rearing decision and experiences of unmarried single childrearing mothers . The main category of their experiences was to 'Overcoming prejudice and forming self-identity as baby's mother' The process of improvement was divided into 'avoidance period' and 'self-leading period.' Unmarried single mothers's child rearing experiences were grouped 'negative neighbors' eyes',
유두상 갑상선암 환자에서 림프절 전이 진단을 위한 미세침세척액의 티로글로불린 측정의 유용성
정경순 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1
Background: The aim of our study was to evaluate the utility of thyroglobulin(Tg) measurement in fine-needle aspiration(FNA) for detecting cervical lymph node metastases from papillary thyroid cancer(PTC). Materials and Methods: We performed ultrasonography-guided fine-needle aspiration cytology(FNAC) and Tg-FNA for 49 ultrasonographically suspicious lymph nodes in 42 PTC patients. Twenty eight patients were waiting surgery and the remaining 14 patients had undergone thyroid surgery for papillary thyroid cancer. The final diagnosis were determined based on the results of histological examination(n=49). Results: Lymph node metastasis were confirmed in 46 lymph nodes. FNAC detected 39 metastatic lymph nodes, and Tg-FNA detected 41 metastatic lymph nodes. Two lymph nodes with a negative results on both methods had metastatic lymph nodes on the results of histological examination. The sensitivities of FNAC, Tg-FNA and combined both methods were 84.7%, 89.1% and 97.8%, respectively. Conclusion: Ultrasonography with both FNAC examination and Tg measurement in FNA washout is highly sensitive and give important information for detecting lymphnode metastasis in patients with papillary thyroid cancer.
정경순,박용빈,최치용,고기석,최임순,Cheong, Kyung-Soon,Park, Yong-Bin,Choi, Chee-Yong,Koh, Ki-Seok,Choe, Rim-Soon 한국현미경학회 1990 Applied microscopy Vol.20 No.2
Several types of the epithelial cells were classified by ultrastructural observation through transmission electron microscope in the rat epididymis. Ultrastructural studies showed that the principal cells, basal cells and narrow cells are located in all the regions of the epididymis and the light cells are present only in the corpus and cauda epididymis. It was observed that the columnar epithelial cells like the principal cells, light cells and narrow cells contain several secretory vesicles and there are halo cells migrating in the several regions. The basal cells showed the elliptical forms in the caput and corpus region and the global forms in the cauda region.
정경순 김천과학대학 2008 김천과학대학 논문집 Vol.34 No.-
Purpose: The purpose of this study is to identified patterns and trend of studies and to analyzed the research related to the nursing intervention for mother-child interaction promotion in Korea Method: The research studies related to the nursing intervention for mother-child interaction promotion were selected from the Korean Nurses Academic Society Journal and dessertation which were conducted experimental research design the last 8 years in Korea. The total numbers of the studies were 17. These studies were classified according to 1)time to publication, 2)participants of studis , 3)type of nursing intervention for mother-child interaction promotion , 4) Measure variables and Effect of nursing intervention, 5)measurement tool. Result: The research related to the nursing intervention for mother-child interaction promotion were 4(24%) from the doctoral dissertation, 4(24%) from the Master's theses and 9(52%)other papers were published in academic journals. 2)Participants of these studies were mother-infant 11(64%), prmipara-infant 3(18%), mother-neonatal 1(6%), mother-infant with low birth weight 1(6%), mother-fetus 1(6%). 3)Type of nursing intervention for mother-child interaction promotion was sensory stimulation 11(64%), maternal education 5(29%), mother-fetus interaction promotion programe 1(6%). 4)Effect of nursing intervention were greatly positive influence on mother aspect which was maternal self-confidence, maternal self-esteem and satisfaction in maternal-role performance and infant aspect which was increased body weight, childrearing environment, infant temperament. 5)The measurement tool was used Bamard(1978)'s NCAFS(Nursing Child Assessment Feeding Scale) on 5studies, Walker, Crain & Thompson(1986)'s MIPIS(Mother-Infant Play Interaction Scale on 4studies and instrument developed by Kim(2000) on 4studies. Conclusion: Therefore we make following which are made based on the above research analysis. 1) In the future research studies need to analysis with other area of discipline in intervention for mother-infant intraction promotion 2) It needs to be developed of maternal-infant measurement tool which is acceptable in Korea society. 3) It needs to the integrated by meta analysis which is to be verified effect's size on massage to be used for maternal-infant intraction promotion.