한약재를 이용한 뇌신경전달물질 및 뇌신진대사에 관한 연구

황유연,이택준,유병수,장두섭,이기남 한국전통의학연구소 2001 한국전통의학지 Vol.11 No.1

Acetylcholinesterase(AChE) is an enzyme which hydrolyses acetylcholine into choline and acetate. Glucose and Adenosine-5'-Triphosphate(ATP) are the primary energy source for the brain and therefore defects in glucose metabolism and the mitochondrial system can affect cognitive function. The fact that the level of acetylcholine(ACh), neurotransmitter, glucose and ATP, are found to be decreased in the brain of Alzheimer patients has lead us to focus on the inhibition of AChE for the developmental research of the Alzheimer's disease(AD) treatment. In this paper we describe the effects of herbal medicine 9908(滌痰化瘀湯加減) in vitro and in vivo. As herb 9908 concentraion increased, the Vmax values of AChE from Electric eel were decreased, which showed that the activity of AChE in vitro was decreased. Also herb 9908 inhibit AChE activity in vivo. When herb 9908 was orally administrated to rats, amount of glucose and ATP which are energy source in metabolism, increase in brain and blood of rats. These result are also interesting in their effects for AD treatment compare with comercial drugs such as Cognex and Aricept. Furthermore, when considering the toxic problems of the Cognex and the Aricept, these oriental medicines could be very valuable medicine for treatment of AD. Abbreviation AD: Alzhemer's disease, AChE: acetylcholinesterase, ACh: acetylcholine, ATCh: acetylthiocholine, DTNB: 5,5‘-dithio-bis-2-nitrobenzoate, ATP: Adenosine-5'-triphosphate GAPD: Glyceraldehydephosphate dehydrogenase PGK : phosphoglycerate phosphokinase

미생물 발효 영지버섯 추출액의 다당체에 관한 연구

황유연(Yu Yeon Hwang),정명수(Myong Soo Chong),김혜자(Hae Ja Kim),이기남(Ki Nam Lee) 한의병리학회 2007 동의생리병리학회지 Vol.21 No.6

일반연제(포스터) : P-25 ; 자동화 액체배양을 이용한 항결핵제 감수성 검사의 재검유형 분석

정태원,황유연,허희재,이남용 대한임상병리사협회 2013 임상미생물검사학회 발표자료집 Vol.2013 No.-

배경: 우리나라의 결핵발생률은 2011년 기준으로 10만 명당 97명으로 OECD 회원국 중 가장 높고 WHO가 2011년에 발표한 세계 결핵관리 보고서에서 다제내성 결핵 추정환자 또한 1,700명으로 OECD 회원국 중 가장 많았다. 이에 따라 보건복지부는 2020년까지 결핵 발생률을 현재의 절반 수준인 10만 명당 50명으로 줄인다는 목표를 담은 제1기 국가결핵관리 계획(2013~2017년)을 발표하였다. 다제내성 결핵 발생률을 줄이기 위해서는 신속한 결핵 약제 감수성의 결과보고를 통해 적절한 치료가 빠르게 이루어져야 하며, 이 때, 자동화 액체배양을 이용한 감수성 검사는 신속한 결핵약제감수성 보고에 매우 유용한 방법이다. 저자들은 본원에서 시행하고 있는 자동화 액체배양을 이용한 감수성 검사에서 보고 지연의 원인이 되는 재검률을 파악하고 원인을 분석하였다. 방법: 2013년 1월부터 7월까지 결핵균 배양 양성 검체에 대하여 제조사의 지침에 따라 MGIT tube(Becton Dickinson, Sparks, MD, USA)에 균액을 접종 후 BACTEC MGIT 960 system (Becton Dickinson, USA)에서 배양하여 액체배양을 이용한 항결핵제 감수성 검사를 시행하였다. 감수성검사의 결과가 isoniazid (INH), rifampin (RIF)에 모두 내성인 경우, growth control의 성장이 4일미만으로 빠른 경우, 또는 13일 이내에 양성이 아닌 경우 재검을 시행하였다. 감수성 검사의 재검률을 확인하고 재검 종류를 유형별로 분류하였다. 결과: 전체건수 262건 중 환자기준 검체 재검실시 횟수는 90건(34.4%) 있었으며, 1회 이상 추가 검사를 실시한 재검은 2회-26건, 3회-8건, 4회-2건을 포함하여 총 재검 실시 126건(48.1%)이었다. 재검의 유형별 구분으로는 13일 이내에 growth unit이 400에 도달하지 않는 균 미발육 47건(37.3%)이었으며, 고체배지에서 자란 균주에 대하여 검사를 시행하면서 계대배양 후 재검이 필요한 경우가 24건(19.0%)이었다. INH와 RIF에 모두 내성으로 PNB (Para-nitrobenzoic acid)검사를 통해 비결핵항산균 혼재 감별 후 재검이 시행된 경우가 21건(16.7%)이었으며, 균발육이 느려 균종의 혼합의 감별을 위해 계대배양이 필요한 경우가 19건(15.1%)이었다. NTM 혼합배양으로 인하여 4일이내로 빠르게 growth unit이 400에 도달하여 순수 분리 후 재검한 경우가 15건(11.9%)이었다. 고찰: 액체배지를 이용한 MTB-DST 검사는 신속한 결과 보고가 가능하였으나, 재검사 비율이 34%에 이르며 여러 차례 재검사를 시행한 경우를 고려하면 48%로 매우 높은 나타났다. 유형별 분류 중 균 미발육과 고체배지 계대배양에 의한 재검은 검사실 자체 변동요인이며, PNB 검사, 계대배양, NTM 혼합배양은 환자 및 균주별 변동요인으로 판단된다(검사실 변동요인 56.3%, 환자및 균주별 변동요인 43.7%). 자동화 액체배양을 이용한 감수성 검사 재검사율이 높아 추가비용이 발생하고 검사자의 업무가 증가하나 검사 결과 후의 치료 방향의 제시 등의 중요성을 고려 할 때 수익창출을 위한 검사 보다는 다제내성 결핵환자의 격리와 치료경과를 위해 필수 검사라고 생각되며, 검사실 변동요인에 대한 분석과 개선을 통하여 재검사 비율을 줄이는 노력이 필요하겠다.

발백산(경북, 경산)의 관속식물상

황유진,박성준,최수미,황새연,황재식,배성현,박선주 한국자원식물학회 2023 한국자원식물학회지 Vol.36 No.1

Abstract - This study was conducted to study the vascular plants of Balbaeksan, located on the border between Gyeongsan-si and Cheongdo-gun, Gyeongsangbuk-do. It was performed 13 times from September 2020 to November 2021, and the investigated vascular plants were identified as a total of 93 families, 302 genera, 485 species, 10 subspecies, 40 varieties, 13 varieties, and 545 taxa. Endemic plants were investigated in 18 taxa, 9 taxa of rare plants; Vulnerable (VU) 5 taxa, Least concerned (LC) 3 taxa, Data Deficient (DD) 1 taxa, Red List plants 5 taxa; 3 taxa of least concerned (LC), 2 taxa of Not Evaluated (NE), 183 taxa of biological resources subject to export approval, 78 taxa of specific taxonomy; There were 9 taxa of grade IV, 20 taxa of grade III, 20 taxa of grade II, and 28 taxa of grade I. 4 taxa of plants subject to climate change adaptation; 2 taxa of endemic plants, 1 taxa of southern plants, 1 taxa of northern plants, 4 taxa of climate change biomarker species, 2 taxa of candidate species of climate change biomarker, 65 taxa of exotic plants, and 3 taxa of ecosystem disturbance species. Plants 235 taxa (43.1%), medicinal plants 257 taxa (47.2%), ornamental plants 148 taxa (27.2%), woody plants 54 taxa (9.9%), herbaceous plants 37 taxa (6.8%), industrial plants 15 Taxa (2.8%), 11 taxa (2.0%) of fiber-bearing plants, and plants of unknown use were identified as 73 taxa (13.4%). The flora of Mt. Balbaek was investigated for the first time, and plants to be preserved in order to secure useful plant resources and biological sovereignty were identified. So continuous monitoring is necessary to prevent the introduction of foreign plants that may be caused by the recent increase in the number of people coming and going. 적 요본 연구는 경상북도 경산시와 청도군 경계면에 위치한 발백산의 관속식물을 연구하기 위해 수행되었다. 2020년 9월부터2021년 11월까지 총 13회에 걸쳐 수행되었고, 조사된 관속식물은 총 93과 302속 482종 10아종 40변종 13품종 총 545 분류군으로 확인되었다. 특산식물은 18 분류군으로 조사되었으며, 희귀식물 9 분류군; 취약종(Vulnerable, VU) 5 분류군, 약관심종(Least concerned, LC) 3 분류군, 자료부족종(Data Deficient, DD) 1 분류군, 적색목록식물 5 분류군; 관심대상(Least concerned, LC) 3 분류군, 미평가(Not Evaluated, NE) 2 분류군, 국외반출승인대상 생물자원 183 분류군, 구계학적특정종 78 분류군 ; IV등급종 9 분류군, Ⅲ등급종 20 분류군, Ⅱ등급종 20 분류군, Ⅰ등급종 28 분류군으로 나타났다. 기후변화 적응 대상식물 4 분류군; 특산식물 2 분류군, 남방계식물 1 분류군, 기후변화생물지표종 4 분류군, 기후변화생물지표종 후보종 2 분류군, 외래식물 65 분류군, 생태계교란종 3 분류군으로 조사되었으며, 용도별 구성은 식용식물 235 분류군(43.1%), 약용식물257 분류군(47.2%), 관상식물은 148 분류군(27.2%), 목재용식물 54 분류군(9.9%), 목초용식물 37 분류군(6.8%), 공업용식물15 분류군(2.8%), 섬유용식물 11 분류군(2.0%), 그리고 용도를알 수 없는 식물은 73 분류군(13.4%)으로 확인되었다. 발백산의식물상 조사는 국내 최초로 수행 되었으며, 유용한 식물 자원 및생물 주권을 확보하기 위해 보전해야 할 식물들을 확인하였다. 최근 사람들의 왕래가 잦아짐에 따라 초래될 수 있는 외래식물유입을 방지하기 위해 꾸준한 모니터링이 필요할 것으로 생각된다.

CO₂ 레이저와 scalpel을 이용한 위 절개 시 개에서의 창상 치유 평가

이재연,황유선,김한결,최현석,정성목,조성환,박창식,김명철,Lee, Jae-Yeon,Hwang, You-Sun,Kim, Han-Kyul,Choi, Hyun-Suk,Jeong, Seong-Mok,Cho, Sung-Whan,Park, Chang-Sik,Kim, Myung-Cheol 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.2

The objective of this study was to compare wound healing in stomach with $CO_{2}$ laser and scalpel incision by measuring the extent of bleeding, the ease of gastric incision, incision time, degree of adhesion and wound healing degree in dogs. Sixteen healthy dogs were used. Two symmetrical incisions were made in ventral aspect of the stomach between the greater and lesser curvatures were made with scalpel and 0.2 mm spot diameter $CO_{2}$ laser (8W, continuous wave) in sixteen dogs. And then each wound was closed with absorbable suture in a two-layer inverting seromuscular pattern. At 3, 7, 14 and 21 days after initial wounding, each wound was taken for histological observation. On surgery, the extent of bleeding, the ease of incision and incision time showed significant differences between the groups. The $CO_{2}$ laser provided better hemostasis (p < 0.05) and smaller postoperative adhesion compared with the scalpel. However, the scalpel produced faster speed of incision and was easier to handle than the $CO_{2}$ laser (p < 0.05). Although there was no considerable difference between the two groups in histological observation, necrosis and calcium deposit tended to be larger in the $CO_{2}$ laser than in the scalpel.

약물상호작용을 중심으로 한 스타틴계 약물의 약물 사용 평가

최선,황유경,이수경,박은하,한옥연,나현오 한국병원약사회 2013 병원약사회지 Vol.30 No.2

A retrospective, cross-sectional study was conducted to evaluate the actual incidence of statin-related adverse events due to drug-drug interaction (DDI) using the Clinical Decision Supporting System (CDSS) with a DDI alert module. Electronic medical records (EMR) were used to estimate the statin-related harmful DDI. We reviewed the full EMR of those patients (1) who were prescribed with statins from 01 January 2010 to 30 June 2011 and (2) whose prescription had a DDI alert message due to contraindicated concomitant drug use. Among 45,080 statin users, 221 (0.49%) patients had DDI alert messages related to their statins. The most frequently DDI alerted statin was rosuvastatin (189/221, 85.5%), and cyclosporine was the most frequently alerted concomitant medication (190/221, 86.0%). Based on the EMR review, 36 of 221 (16.3%) DDI alerted patients had DDI intervention by increasing the dosing intervals of statin (77.8%, 28/36). Most DDI alerted patients had normal laboratory values in serum creatine kinase (CK) and in the liver function test. For the descriptive EMR records review, 3 patients had muscle symptoms sus-pected to be related to statin DDIs. According to the results, contraindicated drug combinations related to statins and actual statinrelated myopathy were rare. However, most patients prescribed with statins could have multiple comorbid conditions; hence, health care professionals should consider different pharmacokinetic profiles of statins and different spectrum of drug interactions in order to select the optimal statin for the patient. Counseling the patients with regards to the risk and warning signs of myopathy and the possibility of drug interactions is also essential.

응급 공혈자검사의 임상적 유용성

엄태현,한복연,김진규,권석운,황유성,한규섭 大韓輸血學會 1996 大韓輸血學會誌 Vol.7 No.1

유방암조직과세포주에서열충격단백질60 kDa의 발현

유병철,박승연,이영돈,이정남,황유진,박흥규 한국유방암학회 2008 Journal of breast cancer Vol.11 No.4

Purpose: Breast cancer has been reported as the most common cancer of women in the United States, Western Europe and Korea and about 210,000 and 10,000 women in United States and Korea every year, respectively are diagnosed with it. Breast cancer is curable with an early diagnosis, and many researchers have made efforts to find a marker for this malady, heat shock protein (HSP) consists of 6 groups, it is highly preserved throughout both the prokaryotic and eukaryotic cells and it acts as a molecular chaperone that’s involved in protein folding. HSPs have been recently reported to be related with breast cancer. In this study, we investigated the changes of expression of HSP60 in tissues and cell lines of breast cancer. Methods: We obtained breast cancer tissues and normal tissues from breast cancer patients, and we purchased several cancer cell lines from American tissue culture correction. We treated the tissues and the cell lines of human breast cancer with heat shock protein. Proteins and mRNAs were isolated from the tissues and the cells and then we performed Western blotting, reverse transcriptase-Polymerase chain Reaction and fluorescence activated cell sorter analysis on them. Results: On Western blot, HSP60 was more overexpressed in the tissues and the cell lines of breast cancer than in the normal breast tissues and cell lines. The expression of HSP60 showed 2 types of molecular weight differences in the tissues and cell lines of breast cancer, and specifically, low HSP60 was over-expressed in the cancer issues. There was no difference between the breast cancer cell lines and the normal cell lines in the expressions of HSP60 mRNA, according to the treatment with heat shock. Also, there was no relationship between phosphorylation and the structural difference of HSP60 protein, according to HSP60 protein’s molecular weight. The expression of HSP60 has been mostly reported at the mitochondria; however, in this study, it was more predominantly detected at the cytoplasm than at the mitochondria in the breast cancer cell lines. Conclusion: We conclude that HSP60 may be used as a diagnostic marker for breast cancer. Detailed investigation of the usefulness and significance of the HSP60 expression as a prognostic factor is required in further studies.

국토연구원 단신

임정천(편집),황유원(편집),김종진(편집),이민영(편집),어은주(편집),김수연(편집),김종진(편집),손은영(편집),김슬예(편집),박대한(편집),하태훈(편집),이미영(편집),서승연(편집) 국토연구원 2018 국토 : planning and policy Vol.444 No.-

단일 기관에서 장내 기생충 양성률의 변화(2003-2012년)

김영은,허희재,황유연,이남용 대한임상미생물학회 2013 Annals of clinical microbiology Vol.16 No.3

Background: Due to a reduction in the number of parasite infections, attention paid to the importance of intestinal parasites has decreased. However, intestinal parasite infections remain ubiquitous and have reappeared as a growing problem in recent decades due to changing lifestyles such as increased overseas travel. In this study, we evaluated trends in intestinal parasite infection using stool examination in a single institute. Methods: From January 2003 to December 2012, we reviewed all stool examination results performed at Samsung Medical Center. Fecal examinations were performed by formalin-ether sedimentation. Results: A total 429,866 stool examinations were performed resulting in 14,672 cases with positive findings of helminth eggs or protozoan cysts, of which the positive rate was 3.41% on average. The annual positive rate decreased from 5.68% in 2003 to 2.43% in 2012. The positive rate of intestinal parasites, excepting Endolimax nana and Entamoeba coli, was 1.52% on average. Positive rates decreased from 2.13% to 1.10% for helminth egg detections and from 2.55% to 1.30% for protozoan cyst detections during the same time period. Among nematodes, Trichuris tricuria was the most common and had an increasing positive rate after 2010. Clonorchis sinensis was the most prevalent trematode parasite, with an annual average of 528 cases. Conclusion: Infection rates of intestinal parasites have decreased over the last 10 years. However, Trichuris tricuria has reappeared and has become a major contributor to parasite infections. Further education and control efforts are needed for greater prevention and eventual eradication of parasitic infections.