방광의 소세포 신경내분비 암종의 요 세포학적 소견 - 1 예 보고 -

김동훈,강동욱,김경희,김주헌,박미자,Kim, Dong-Hoon,Kang, Dong-Wook,Kim, yuug-Hee,Kim, Ju-Heon,Park, Mee-Ja 대한세포병리학회 2002 대한세포병리학회지 Vol.13 No.2

We report the cytologic features of a case of primary small cell carcinoma of the urinary bladder with high grade transitional cell and signet ring cell carcinomatous components. A 64-year-old male presented with gross hematuria for one week. Computed tomography revealed an ill-defined mass in the left lateral wall of the urinary bladder. Urinary cytology showed hypercellularity with predominantly isolated single cells and clustered cells. They have scanty cytoplasm and naked hyperchromatic nuclei with finely granular nuclear chromatin and rare nucleoli. The tumor cells occurred predominantly singe cells, but a few in clusters. Nuclear molding was prominent. No glandular formation or nesting was noted. The second tumor cells had high nuclear/cytoplasmic ratio, irregular nuclear membrane, and coarse granular chromatin. The background was inflamed and necrotic. The histoiogic findings of transurethral resection were mainly composed of small cell carcinoma, and partly transitional cell and signet ring cell carcinomatous components. Small cell neuroendocrine carcinoma have distinctive cytologic features to make a proper diagnosis.

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF)의 첨가가 생쥐 수정란의 발생과 착상관련 유전자 발현에 미치는 영향

김동훈,고덕성,이회창,이호준,강희규,김태전,박원일,김세웅,Kim, Dong-Hoon,Ko, Duck-Sung,Lee, Hoi-Chang,Lee, Ho-Joon,Kang, Hee-Gyoo,Kim, Tai-Jeon,Park, Won-Il,Kim, Seung-Samuel 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.2

Objective : The purpose of the current series of experiments were to assess the effect of GM-CSF, as a medium supplement, on the development of mouse embryos and the expression of LIF and IL-1? mRNA. Materials and Methods: Mouse 2-cell embryos were collected from the oviducts of 6 weeks old ICR mice at 48 hours after hCG injection. Embryos were cultured in P-1 medium supplemented with mouse GM-CSF (0, 1, 5, 10 ng/ml). The embryo development to blastocysts and hatching blastocysts was assessed and the cell number in blastocyst was also examined. Using RT-PCR, the expressions of LIF and IL-1? mRNA in blastocyst were evaluated in the GM-CSF supplemented group and control group. Results: In mouse, the addition of GM-CSF increased the percentage of blastocysts (65.5%, 68.6%, 73.0% and 76.1% for control and 1, 5 and 10 ng/ml, respectively), and increased the proportion of hatching blastocysts (35.2%, 36.4%, 43.2% and 53.0% for control and 1, 5 and 10 ng/ml, respectively). The mean cell numbers in blastocyst were significantly increased in GM-CSF supplemented groups compared to control group. LIF and IL-1? expression in blastocyst were significantly higher in GM-CSF supplemented group than in control group. Conclusion: The results of experiment by mouse embryos showed beneficial effects of GM-CSF as a medium supplement. Furthermore, the addition of GM-CSF significantly increased the expression of LIF and IL-1? in mouse embryos. These results suggest that GM-CSF might be a important molecule in embryo implantation.

한열성향(寒熱性向)에 따른 신문혈(神門穴) 애구(艾灸) 효능(效能)의 비교(比較) 연구(硏究)

김동훈,김종덕,김은정,김경태,류성룡,정지철,박영배,Kim, Dong-hoon,Kim, Jong-deog,Kim, Eun-jung,Kim, Kyung-tae,Rhu, Seong-ryong,Jung, Ji-chul,Park, Young-bae 대한침구의학회 2004 대한침구의학회지 Vol.21 No.4

Objective: Moxibustion is one of major healing technique in oriental medicine. It has been widely used in many disease. There is an text which suggest moxibustion is more efficient to the situation of han(寒) and heo(虛) than yeal(熱) and sil(實) in Huangdineijing <黃帝內經>. The aim of this study is to research the effect of moxibustion at Shinmun(H7) is different according to cold or heat tendendy man by analyzing the electroencephalogram(EEG). Methods: We classified objects by their cold or heat tendency using questionnare for cold - heat patternization. (12 cold tendency man, 19 heat tendency man) Before and after moxibustion at Shinmun(H7), EEG raw data were measured during 5 minutes. The correlation dimension(D2), the correlation dimension variability rate(${\Delta}D2$), largest lyapunov exponent(L1) and largest lyapunov exponent variability rate(${\Delta}L1$) were calculated. We analyzed D2, ${\Delta}D2$, L1, ${\Delta}L1$ to see the effect of moxibustion at Shinmun(H7) was statistically different according to Cold or Heat tendendy man. Results : Paired t-test showed significant differences between before and after moxibustion at Shinmun(H7) on the Fp2 in D2(p<0.05), on the Fp2, F3 and F4 in ${\Delta}L1$(p<0.05). Student Hest showed significant differences between cold and heat tendendy man on the F3 in ${\Delta}L1$(p<0.05). Conclusion: These results suggest that moxibustion at Shinmun has an effect on stabilizing mind and it is more efficient to the cold tendendy man than the heat tendendy man.

리튬이온이차전지용 LiCoO₂-유기전해액의 충전상태에 따른 열적 안정성

김동훈,이영호,신혜민,정영동,도칠훈,진봉수,김현수,문성인,오대희,김기원,Kim, Dong-Hun,Lee, Young-Ho,Shin, Hye-Min,Chung, Young-Dong,Doh, Chil-Hoon,Jin, Bong-Soo,Kim, Hyun-Soo,Moon, Seong-In,Oh, Dae-Hui,Kim, Ki-Won 한국전기전자재료학회 2007 전기전자재료학회논문지 Vol.20 No.5

Thermal behavior of $Li_{1-x}CoO_2$ has been investigated employing DSC (Differential Scanning calorimetry) and TGA (Thermogravimetry Analyzer), and the crystal parameters were calculated from XRD (X-ray diffraction).for the commercial rectangular pouch cell(1000 mAh).The cathode materials coated over aluminium foil current collector is made up of a blend consisting of active material $LiCoO_2$(size $20\;{\mu}m$, 94 wt%), conducting material super p black (SPB, 3 wt%) and binder polyvinylidene fluoride (PVDF, 3 wt%). The anode is a mix consisting of carbon (92 wt%) and PVDF(8 wt%) coated over copper foil. The cells for the experiments were first preconditioned by cycling three times and stabilized at OCV=3.0, 3.5, 4.2, 4.35 and 4.5 V. The stabilized cathode material was used for thermal and crystal parameter investigations.

어리장 형태와 계절이 육계 출하환경 및 폐사에 미치는 영향

김동훈,박범영,황인호,조수현,김진형,이종문,Kim Dong-Hun,Park Beom-Young,Hwang In-Ho,Cho Soo-Hyun,Kim Jin-Hyung,Lee Jong-Moon 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.1

이 연구는 어리장 형태와 계절이 출하환경 및 폐사율에 미치는 영향을 조사하여 출하 중 폐사에 의한 경제적 손실을 예방하고 관련 시설 개선을 위한 정책적 자료를 확보하기 위해 수행하였다. 관리실태는 출하관리환경이 다른 2계열 업체의 관리대장을 입수하여 1, 4, 8, 10월 첫째주 1주일분에 대해 적재중량, 수송밀도, 수송 및 계류시간, 폐사율 등을 분석하였다. 두 업체의 평균 적재중량, 수송시간, 계류시간 및 폐사율은 각각 3.9 톤, 96 분, 478 분 및 0.6%이었다. 상자형과 컨테이너형 어리장의 수송중 폐사율은 각각 0.5와 0.6%로 컨테이너형이 유의적(p<0.05)으로 높았으며 적재중량 및 수송밀도($cm^2/kg$)또한 컨테이너형이 상자형에 비해 많고 높았다. 출하계절별 폐사율은 봄, 겨울이 각각 0.7, 0.8%로 여름, 가을의 0.5%에 비해 유의적으로 높은 경향을 나타내었다. 어리장 형태와 출하계절에 따른 폐사율은 상자형의 경우 봄, 겨울이 각각 0.8, 0.7%로 여름, 가을의 0.3, 0.4%에 비해 높았다. 컨테이너형은 봄, 가을, 겨울의 폐사율이 각각 0.7, 0.5, 0.8%로 유의적인 차이를 보였으며 봄과 여름, 여름과 가을은 유의적인 차이를 보이지 않았다. 출하 후 육계의 폐사율은 컨테이너형, 상자형이 비슷하였으며 계절은 봄, 겨울이 여름, 가을에 비해 높았다. 이는 다른 계절에 비해 계류장에서의 대기시간이 길고 가온시설이 없어 동사한 닭이 많기 때문으로 사료되었다. The current study was conducted to investigate the effect of types of crate and season on transport condition and mortality, and ultimately to identify the best practice for reducing economic lose. Total loading weight stocking density, transport and lairage times and mortality were surveyed from the management data sheet of two companies for each first week of January, April, August and October, An average loading weight, length of transport and lairage times and mortality were 3.9 ton, 96 min, 478 min and 0.6%, respectively. Mortality after lairage was not significant between two types of crate. In addition, container type crate showed higher loading weight and stocking density than box type one. Spring and winter had significantly higher mortality with 0.7 and 0.8%, respectively, then summer and fell of 0.5%. An interaction between crate type and season on mortality showed that mortality for box type on was higher in spring and winter with 0.8 and 0.7%, respectively, compared to summer and fall of 0,3 and 0.4% respectively. In the case of container type crate, spring, fall and winter had greatly different death into with 0.7, 0.5 and 0.8%, respectively, while there was no difference between spring and summer, and between summer and winter, Mortality after transportation was similar between both crate type, with higher rate for spring and winder than other seasons. The result was likely related to death of exposure due to extended waiting time without heating facility.

레이져를 이용한 부분적 보조부화술이 생쥐 수정란의 부화에 미치는 효과

김동훈,김묘경,이회창,고덕성,박원일,권혁찬,이호준,Kim, Dong-Hoon,Kim, Myo-Kynng,Lee, Hoi-Chang,Ko, Duck-Sung,Park, Won-Il,Kwon, Hynck-Chan,Lee, Ho-Joon 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.2

Objective: The present study was performed to investigate the efficiency of partial laser assisted hatching (p-LAH; lased 1/2 ZP width from ZP edge) on hatching of mouse blastocysts. Methods: We used non-contact $1.48{\mu}m$ diode laser (MTM, Switzland) to create a precise hole on zona pellucida. 2-cell embryos were collected from the mouse (ICR) oviduct at 48 hours after hCG administration. Collected 2-cell embryos were cultured in the P-1 medium supplemented with 0.4% BSA. For experiments, embryos at 8-cell stage were used after $20{\sim}22$ hours in culture. After conventional (c-LAH) or partial laser assisted hatching, the embryos were further cultured in P-1 medium supplemented with 0.4% BSA for 3 days. To compare efficiency of complete and partial laser assisted hatching, hatching rate, hatching time and blastocyst diameter and zona pellucida thickness at hatching time were investigated. Embryos were examined every 12 hours. Blastocyst diameter and zona pellucida thickness at hatching time were measured with an ocular micrometer. Results: Hatching rates of p-LAH group (84.2%) was significantly higher than that of control group (39.3%), but there was no difference between the p-LAH (84.2%) and c-LAH (91.2%). p-LAH group was hatched 12 hours earlier than control group, but hatched 12 hours later than c-LAH group. The diameter of blastocyst at hatching time of p-LAH group ($113.1{\pm}6.4{\mu}m$) was smaller than that of control group ($122.2{\pm}5.0{\mu}m$), but larger than that of c-LAH group ($102.2{\pm}2.7{\mu}m$). Zona pellucida thickness at hatching time of p-LAH group ($6.4{\pm}0.9{\mu}m$) was thicker than that of control group ($4.5{\pm}1.5{\mu}m$), but thinner than that of c-LAH group ($10.0{\pm}0.8{\mu}m$). Conclusion: These results suggest that p-LAH may maintains the cell arrangement of early embryos to ensure successful development and prevent precocious hatching of blastocyst when compare to c-LAH and conventional (acidic tyrode) AH. Thus, p-LAH may provide a valuable and effective AH technique for human ART program.

M2M(Mobile to Machine) 환경에서의 유비쿼터스 기반 공작기계 운용기술

김동훈,송준엽,Kim, Dong-Hun,Song, Jun-Yeop 재료연구소 2007 機械와 材料 Vol.19 No.1

개방형 AS/RS 운영시스템의 설계 및 적용기술

김동훈,송준엽,이승우,Kim, Dong-Hun,Song, Jun-Yeop,Lee, Seung-U 재료연구소 1998 機械와 材料 Vol.10 No.1

교전수준 잠수함 표준모델 개발 방안 연구

김동훈,신지환,김찬기,Kim, Dong-Hun,Shin, Ji-Hwan,Kim, Chan-Ki 한국시뮬레이션학회 2007 한국시뮬레이션학회 논문지 Vol.16 No.4

본 논문에서는 미래의 수중 교전 시뮬레이션에서 공통적으로 사용될 수 있도록 재사용성 및 상호운용성과 공학 시뮬레이션 등 앞으로의 확장성을 고려하여 수중 교전에 사용되는 잠수함 체계에 대한 표준모델을 어떠한 개념에서 어떠한 형태로 정립할 것인가에 대해 기술하였다. 본 연구를 통해 교전 수준 잠수함 체계 표준모델에 대한 모델아키텍쳐를 정립하고 개별 구성요소에 대한 모델링 방안을 개발하였으며 모델의 입출력 자료 및 정보 흐름도를 도출하였다. 본 연구에서는 비음향 탐지 모델 및 비음향 스텔스 모델 등 수중 교전에 해당하지 않는 분야는 제외하였으므로 향후 이에 대한 추가 연구가 필요하다. This paper describes and explains that the engagement level model of submarine can be used in underwater engagement simulation taking reuse, interoperability, and extension to the engineering leveled simulation into consideration. WBS(Work Break-down Structure), internal data interface, and I/O data about the engagement level model of submarine are developed. It describes the modeling method of components of submarine model as well. In this paper, the non-acoustic detection and stealth models are excluded because those models are not related to the underwater engagement, so the excluded models need to be developed in the future.

Detection of Representative Enteropathogenic Bacteria, Vibrio spp., Pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, Using a Virulence Factor Gene-Based Oligonucleotide Microarray

김동훈,Bok-Kwon Lee,Yong-Dae Kim,이성근,Young-Chang Kim 한국미생물학회 2010 The journal of microbiology Vol.48 No.5

Rapid identification of enteropathogenic bacteria in stool samples is critical for clinical diagnosis and antimicrobial therapy. In this study, we describe the development of an approach that couples multiplex PCR with hybridization to a DNA microarray, to allow the simultaneous detection of the 10 pathogens. The microarray was synthesized with 20-mer oligonucleotide probes that were designed to be specific for virulencefactor genes of each strain. The detection limit for genomic DNA from a single strain was approximately 10 fg. In the presence of heterogeneous non-target DNA, the detection sensitivity of the array decreased to approximately 100 fg. We did not observe any non-specific hybridization. In addition, we successfully used this oligonucleotide-based DNA microarray to identify the causative agents in clinical stool samples from patients with food-borne enteritis.