한국산 해태의 위생학적 연구 2

박대성,조현영,김광호 한국미생물학회 1970 미생물학회지 Vol.8 No.2

In continuation of the previous work (The New Medical Journal, Vol. 12, No. 3, 111, 1969), the effects on the bactericidal activity against coli form group, on vitamin C content and ascorbate oxidase activity of the purple laver due to the $^{80}Co$ gamma-irradiation were studied. The results obtained are ; 1) After the 0.1m rad./hr. doses treatment of gamma-irradiation for 1 hours to the laver, the coli form group was being remarkably destoryed and after the application for 10 hours the coli form group was completely destroyed. 2) The content of vitamin C was gradually decreased during the gamma-irradiation to the laver. According to the sensory test, no changes in flavor nad color were indicated for 9-10 hours treatment. But, the loss of ascorbic was much than that of dehydroascorbic acid after 10-hour treatment. 3) And also, the ascorbate oxidase activity due to the irradiation waas conspicuously decreased.

특별강연발표 초록

학회자료 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1986 한국미생물·생명공학회지 Vol.14 No.4

Pseudomonas sp. CB-33이 생산하는 β-Xylosidase의 특성

유진환,김현구,김치경,임재윤 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

Pseudomonas sp. CB-33 균주의 배양 상등액으로부터 ammonium sulfate 침전법, PEI 침전법, DEAE-Sephadex column chromatography, gel column chromatography 그리고 preparative disc gel electrophoresis를 이용하여 β-xylosidase를 정제하였다. 정제된 효소의 Km 값은 4.6 mM이었다. 효소활성의 최적 pH는 7.0이었으며 pH 6.5~9 범위에서 안정하였다. 최적 활성온도는 45℃였으며, 각 온도에서 30분 동안 정치하였을 때 35℃까지 안정하였다. SDS-PAGE에 의해 효소의 분자량을 측정해 본 결과 약 44,000dalton이었다. HG^2+, Cu^2+ 그리고 Zn^2+는 효소활성을 저해하였고, 여러 화학변형제로 효소에 처리해 본 결과 tyrosine과 tryptophan이 효소활성에 관여하고 있을 것으로 추정된다. 여러가지 기질에 대한 특이성을 조사해 본 결과 pNPX 뿐만 아니라 pNPA도 분해하였다. Xylan에 endoxylanase만을 작용시켰을 때보다 β-xylosidase와 endoxylanase를 혼합작용 시켰을 때 xylan의 가수분해도는 약 2배 정도 증가하였다. 그리고 Pseudomonas sp. CB-33 균주가 생산하는 xylan 분해효소는 oat spelt xylan보다 birchwood xylan에 보다 효과적으로 작용하였다. The β-xylosidase was purified 99-fold from the culture supernatant of Pseudomonas sp. CB-33 by ammonium sulfate precipitation, PEI precipitation, DEAE-Sephadex column chromatography, Sephadex G-75 gel filtration chromatography and preparative disc gel electrophoresis. Molecular weight of the enzyme was estimated to be 44,000 by SDS polyacrylamide gel electrophoresis. The enzyme has a pH optimum for activity at 7.0 and is stable over pH 6.5~9.0. The optimal temperature of the enzyme was 45℃, and it enzymatic activity was completely inactivated at 55℃ for 30 min. Km value of the enzyme for p-nitrophenyl- β-D-xylopyranoside was calculated to be 4.6 mM. The effect of various reagents on the β-xylosidase activity was investigated. The enzyme activity was completely inhibited by Hg^2+, Cu^2+, and Zn^2+. The β-xylosidase was inactivated by tryptophan-specific reagent, N-bromosuccinimide and the enzyme was competitively inhibited by xylose. The β-xylosidase and endoxylanase from Pseudomonas sp. CB-33 hydrolized xylan synergically. The purified enzyme also showed α-L-arabinofuranosidase activity.

한국형 Bifidobacterium longum SPM1205 혼합제제의 변비 개선 효과

김정래,이도경,백은혜,안향미,양환진,김미진,최경순,윤미은,정이정,오복자,정명준,하남주,Kim, Jung-Rae,Lee, Do-Kyung,Baek, Eun-Hye,An, Hyang-Mi,Yang, Hwan-Jin,Kim, Mi-Jin,Choi, Kyung-Soon,Yun, Mi-Eun,Jung, Yi-Jung,Oh, Pok-Ja,Chung, Myung-Jun 한국미생물학회 2010 미생물학회지 Vol.46 No.1

The aim of this study was to evaluated the efficacy of mixture of Lactic Acid Bacteria (LAB) and bifidobacteria supplement, which are contained with Lactobacillus acidophilus, Bifidobacterium longum SPM1205, and Pediococcus pentosaceus for the management of constipation in animal model and clinical trials. 5 ICR mice and 4 female constipation subjects were orally taken mixture of LAB and bifidobacteria for 2 weeks. We investigated the number of fecal LAB and harmful enzymes activities before and after mixture of LAB and bifidobacteria application. As a result, fecal LAB count was increased and harmful enzymes activities of intestinal microflora were generally decreased after mixture of LAB and bifidobacteria application. Also, 61 female subjects were randomly assigned to receive either mixture of LAB and bifidobacteria or lactose and were taken three times a day for 2 weeks. Then, we analyzed mixture of LAB and bifidobacteria effect through the questionnaires. Daily consumption of this mixture of LAB and bifidobacteria improved the constipation in constipation group (56.3%) compared with lactose application group (26.7%). Furthermore, after mixture of LAB and bifidobacteria treatment, frequency of hard stool decreased from 0.22 to 0.03. These results indicated that mixture of LAB and bifidobacteria application is effective to improve the constipation.

한국산 Aspergilli의 혈청학적 분류방법

문희주,이배함 한국미생물학회 1974 미생물학회지 Vol.12 No.4

Of the Asp. spp. isolated by the Institute of Applied Microbiology, Kon-Kuk University, 7 strains were selected for the study of the immunological differencences among them using gel precipitation test. The strains were the following types : 1 type of flavus and 2 types of oryzae were isolated from Meju ; 1 type of flavus from Nuruk ; and each one type of flavus, parasiticus and oryzae from Kokja.Asp.flavus from ATCC, Asp. parasiticus nad Asp. niger NRRL strains were also used in the study as a standard. From this study, several points can be raised ; 1) There was no common antigenic property between Asp. niger and Asp. flavus, because of no formation of reaction line. Therefore, all strains could be easily distinguished. 2) There was common antigenic property, that is, the formation of reaction line between Asp. flavus and Asp. parasticus. Accordingly two strains could not be easily distinguished by the gel precipitation test. 3) Each type of oryzae, parasiticus and flavus of Asp. flavus group had common antigen one another as well as specific antigens only in the difference of the reaction lines, so they could be easily identified in the gel precipitation test. 4) Each isolated strain from Meju and Nuruk appeared to be identical. 5) It was shown that the gel precipitation test of serological methods was very useful for the classification of Asp. spp.

한국산 팽나무 버섯의 계통류별과 그 배양적 특성에 관한 연구

윤정구 한국미생물학회 1974 미생물학회지 Vol.12 No.4

In order to investigate morphological and cultural characteristics of strains in species, Flammulina velutipes, the author collected isolates of Flammultina velutipes at 49 locations in Korea and cultivated these isolates on the various kinds of solid media. After investigating the cultural characteristics, appeared on the various media, he obtained the following results : 1. The variation of colors in the fruit bodies is connected with the variation of climatic environments(composite effects between mean temperature in January and number of rain days of 1mm and over precipitation). The author, therfore, can find out the trend that brown type is distributed in the midland climatic region and yellow type in the southern climatic regoin. 2. Two types can be classified into several strains respectively : the strain of abundant or insufficient productivity, and strain of selectivity or non-selectivity of media. 3. According to the results of mutual comparison of soluble mycelial proteins by disc electrophoresis using polyacrylamide gels, each type has special common protein fractions(brown type : band located at 26..5mm position from surface of gel, yellow type : band located at 24.5mm position from surface of gel), and each strain has special protein fractions too. Therefore this phenomenon seems to support the results obtained by the above-stated morphological and cultural studies. 4. In the adaptability of strains to the temperature, every strain has the nature of growing in lower temperature(the optimum temperature of 20.deg.C to 25.deg.C) except that YI-1 strain has the optimum temperatue of $25^{\circ}C$-26^{\circ}C. And mycelial growth of every strain is discontinued at $35^{\circ}C.$ 5. In the adaptability of strains to the H-ion concentration, every strain has wide adaptable range of H-ion concentration, and has optimum range of pH 5.5 to 6.6 in mycelial growth excepting YA01, BI-2 and YI-1. 6. In the utility of carbon sources, the mycelial growth of every strain is very poor on the media containing xylose(average diameter of mycelial growth : 18mm), and most strains utilize favorably sucrose(39mm), maltose(37mm) and dextrose(35mm) in mycelial growth. In the utility of nitrogen sources, every strain utilizes favorably organic nitrogens(36 mm)more than inorganic nitrogens (25 mm), and utilizes fully peptone nad asparagine in organic nitrogens. Especially BA-1, BIK-2 and YA-1 strains grow vigorously on each media containing various carbon and nitrogen sources. 7. The characteristic tests of the number of days required for mycelial growth, the number of days requried for sprout of young bodies, the length of stipe and the number of fruit bodies formation seem to be useful methods in the early selection of the strain of the abundant productivity.

쑥 추출물의 항돌연변이 활성효과

이성,권동진,유진영,정동효 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

한국산 쑥으로부터 얻어진 ethanol 추출물과 각 용매획분에 대하여 in vitro로서 Salmonella typhimurium reversion assay 시험을 하여 자체 변이원성 유무와 4가지 양성 변이원 물질(3㎍/plate 2NF, 2㎍/plate AFB_1, 1㎍/plate Trp-P-1 그리고 1㎍/plate Trp-P-2)에 대한 항돌연변이 활성에 대한 쑥의영향을 고찰하였다. 쑥의 ethanol 추출물은 2NF, AFB_1, Trp-P-1 그리고 Trp-P-2 등으로 유도한 돌연변이 시험(Ames test)에서 변이원성을 보여주지 않았다. 돌연변이 억제시험에서는 frameshift type인 Sal. typhimurium TA 98에 대하여 직접 변이원인 2-NF에서는 19.7~22.9%, 간접 변이원인 AFB_1, Trp-P-1과 Trp-P-2에서는 각각 47.9~61.2%, 64.1~70.7% 그리고 67.4~78.7%의 억제효과를 나타내었다. 또한 쑥의 ethanol 추출물을 n-hexane, chloroform, ethyl acetate 그리고 water로 각각 분획하여 직접변이 원인 2-NF와 간접변이원인 AFB_1을 사용하여 변이원성과 항돌연변이원성 시험을 하였다. 돌연변이 시험에서 변이원성은 4가지 획분에서 모두 나타나지 않았다. 직접 변이원인 2-NF로 유도된 돌연변이에 대하여 n-hexane 획분은 9.4~33.7%, chloroform 획분은 21.6~26.5% 그리고 ethytl acetate 획분은 2.4~21.9%를 나타내었다. 간접 변이원인 AFB_1로 유도된 돌연변이에 대하여 n-hexane 획분, chloroform 획분 그리고 ethytl acetate 획분들은 각각 63.0~80.0%, 77.5~82.1%, 68.5~83.1%로 높은 억제 효과를 보여주었다. Mugwort has been know as a traditional substitutive foodstuff and as showing a physiologically beneficial function to a human being. Therefore, effect of mugwort extract in terms of mutagenicity and desmutagenicity was investigated to verify its function. Ethanol extract from mugwort did not exhibit any mutagenicity. On the contrary, inhibitory effects of the ethanol extract were observed on mutagenicity induced by aflatoxin B_1(AFB_1), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole(Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3]indole(Trp-P-2) and 2-nitroflourene(2NF) using Salmonella typhimurium reversion assay. On direct-acting mutagen(2NF, 3 ㎍/plate), ethanol extract showed a slight inhibitory effect of 19.7%~22.9%, however on indirect-acting mutagen such as AFB1(2 ㎍/plate), Trp-P-1(1 ㎍/plate) and Trp-P-2(1 ㎍/plate), we observed higher inhibitory effect of 47.9~61.2%, 64.1~70.7%, 67.4~78.7%, respectively. Step-wise fractionation of the ethanol extract was done by using hexane, chlorofractions showed high inhibition of 63.0~80.0%, 77.5~82.1%, and 68.5~83.1%, respectively on the mutagenicity of AFB_1 in Sal. typhimurium TA98. Consequently, these results indicated that mugwort extract contains some compound(s) which may show desmutagenicity.

젓갈류

이응호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1988 한국전통발효식품연구의 현황과 전망 Vol.- No.-

1975년도 추계학술발표회 초록

학회자료 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1975 한국미생물·생명공학회지 Vol.3 No.3

유산균 투여가 건강한 성인의 분변미생물 및 부패산물 생성에 미치는 영향

신명수,김용재,배형석,백영진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

유산균의 장내균총과 부패산물 생성에 미치는 영향을 알아보기 위하여, 건강한 성인 남녀 6명의 지원자에게 Lactobacillus acidophilus 와Bifidobacterium longum으로 제조한 유산균분말(각각 1.5×10 exp (9) cells)을 하루에 2회씩 2주동안 섭취하도록 하였다. 그리고 지원자 분변으로부터 균총수 변화와 부패산물인 암모니아, indole, skatole, p-cresole량을 측정하였다. 유산균의 투여 기간중에 분변내 bifidobacteria의 수가 8.78±0.39(log cfu/g feces±S.D)에서 9.27±0.29로 증가하였고(p<0.05) 투여 중지후에는 약간 감소하였으며, lactobacilli의 수는 6.15±0.80에서 6.76±0.48로 증가하였다(p<0.05). Enterococcus 균수는 투여 기간중에 6.66±0.80에서 7.72±0.40으로 증가하였다가 투여 중지후에는 감소하는 경향을 보였다. 그리고 혐기성균인 Bacteroides는 투여중에 8.45±0.34에서 9.15±0.21으로 증가하였으나(p<0.01), 유해균주로 알려진 Closridium(lecithinase negative)과 Staphylococcus 균수는 현저히 감소하였다. 그러나, 유산균 투여 실험기간인 6주 동안 분변내 암모니아, indole, skatole, p-cresole의 유의적인 농도변화는 없었다. To investigate the effects of lactic acid bacteria administration on fecal microflora and putrefactive metabolites in human being, Lactobacillus acidophilus and Bifidobacterium longum powder (1.5×10 exp (9) cells, respectively) was administrated to six healthy volunteers (average 28 years old) twice a day for 2 weeks. During the administration of lactic acid bacteria, the numbers of bifidobacteria, lactobacilli, and enterococci in feces were increased significantly, whereas those of Staphylococcus and lecithinase-negative Clostridium were decreased considerably. In addition, a number of anaerobic Bacteroides were increased. However, the contents of fecal ammonia and putrefactive metabolites (indole, skatole, p-cresole) were not changed during the administration.