Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

최근 30년간 스포로트리쿰증 임상 병형의 추세에 대한 관찰

임채성,권경술,장호선,정태안,오창근 대한의진균학회 1997 대한의진균학회지 Vol.2 No.1

Background: The clinical types of sporotrichosis are generally classified into four group: lymphocutaneous, fixed cutaneous, and cutaneous disseminated, and hematogenous. Of these, the lymphocutaneous variety is the most frequently encountered pattern, constituting 80% of sporotrichosis patients and the fixed cutaneous sporotrichosis is the next. Potassium iodide (KI) was the most commonly used drug for the treatment of sporotrichosis. Many studies about these clinical findings of sporotrichosis have been reported, but comparative study on the changes in clinical findings has not been reported. Objective: The purpose of this study is to clarify the changes of the clinical findings in sporotrichosis in Korea. Methods: A total of 58 patients with sporotrichosis who had visited the Department of Dermatology, Pusan National University were divided into 2 groups according to the period and then the clinical findings of sporotrichosis were compared. Results: The results of study are summarized as follows: 1. During this period, there were 58 cases of sporotrichosis. In the past period (1967~1979) there were 35 cases of sporotrichosis, and there were 23 cases in the recent period (1980~1997) 2. In the past period, most cases (71.4%) were lymphocutaneous type, however in the recent period, most cases (69.2%) were fixed cutaneous type. 3. The most commonly affected site was right upper extremity in the past period, but face fright upper extremity were the most commonly affected sites in the recent period. 4. In the past period, 2nd and 3rd decades were prevalent age group, but 5th to 7th decades were prevalent age group in the recent period. 5. In the treatment of sporotrichosis, potassium iodide (KI) was the choice of treatment in the past period, however itraconazole is gradually used in the recent period. Conclusion: According to this study, many changes in clinical findings of sporotrichosis were noted, especially in the clinical type. We found that clnical type of sporotrichosis changed from lymphocutaneous type to the fixed cutaneous type with time. And we suggest that further observation would be necessary to define the meaning of these changes.

Finding key vulnerable areas by a climate change vulnerability assessment

Kim, Ho Gul,Lee, Dong Kun,Jung, Huicheul,Kil, Sung-Ho,Park, Jin Han,Park, Chan,Tanaka, Riwako,Seo, Changwan,Kim, Ho,Kong, Wooseok,Oh, Kyusik,Choi, Jinyong,Oh, Young-Ju,Hwang, Gangseok,Song, Chang-Keun Springer Netherlands 2016 Natural hazards Vol.81 No.3

<P>Extreme climate events such as typhoons, heat waves, and floods have increased in frequency with climate change. Many municipalities within the Republic of Korea (ROK) have experienced damage from these events, necessitating countermeasures. Vulnerability assessment has been suggested in the implementation of a national plan for reducing damage resulting from climate change. Thus, in this study, we assess the vulnerability of the ROK and identify key vulnerable municipalities in support of the national adaptation plan. We create a framework for assessing the vulnerability of all 232 municipalities of the ROK with respect to 32 items in 7 fields. The framework regards decision makers' comprehension and availability of data as important factors. We assess the vulnerability index of each municipality by using variables of climate exposure, sensitivity, and adaptation capacity. The weights of variables are determined by the Delphi method. We used the representative concentration pathways 8.5 climate scenario to reflect future climate exposure for the vulnerability assessment. From the analysis, vulnerability maps are prepared for the 32 items of 7 fields, and key vulnerable municipalities are identified by aggregating the maps. The distribution of vulnerable municipalities changes with the future climate conditions. These maps provide a scientific and objective basis for the ROK government to establish adaptation plans and allocate resources. The ROK government can utilize the results to identify the characteristics of highly vulnerable areas, and municipalities can use the results as a basis for requesting support from the national government.</P>

경상남도 창녕에서 집단 발생한 피부 탄저병의 임상양상

박찬원,박정래,정종윤,이선희,장철훈,오차재,조병만,임재영,오창근,권경술 대한감염학회 2002 감염 Vol.34 No.4

土壤內 植物奇生線蟲의 保管溫度 및 分離方法에 關한 硏究

吳昌根 건국대학교 1981 論文集 Vol.13 No.1

This study was undertaken with the following objectives; (I) to determine the effects of storage temperature and duration on survival of plant parasitic nematodes, and (II) to compare the effectiveness of the Baermann funnel method and sugar flotation sieving method in extracting nematodes from soil samples exposed to various storage temperature. The obtained results are summarized as follows; (I) Storage of nematodes in soil at -10℃ 1 to 16 weeks greatly increased nematode recovery by the sugar flotation sieving method, but one week exposure to -10℃ killed all nematodes except Pratylenchus sp. and Tylenchorhynchus sp. by the Baermann funnel method. (II) Numbers of each species recovered from samples stored at 15,25,30℃ were similar to those for 10℃ or slightly lower. (III) Generally, when method extracted by sugar flotation sieving procedure were employed, soil samples had better be stored at -10 or 10℃. (IV) When method depending on nematode motility, such as the Baermann funnel method, soil samples had better be stored at 10℃. (V) Therefore, optimum storage temperature for survival of most nematode species was 10℃.

조갑에 국한된 편평 태선에 의한 익상편 1예

오창근,권경술,장호선,이채욱 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.4

Patients with lichen planus may present with nail changes. The nail changes often present are ridging, longitudinal grooving and splitting of the nail plate, and atrophy of the nail. Pterygium formation is to be rare. Lichen planus limited to the nails without skin or mucous membrane involvement is rarely reported in the literature. A case of pterygium due to lichen planus limited to the nails in a 32-year-old man is reported.

각종 단백질 사료의 반추위내 분해도 및 하부 장기내 소화율에 관한 연구

오영균,양시용,김창원,정재준,박근규 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

본 연구는 반추가축사료의 단백질 이용효율을 높이기 위한 방안으로 국내 사료회사에 주로 이용하고 있는 단백질 사료의 반추위내 분해단백질(RDP)과 비분해단백질(UDP) 및 소장에서도 흡수되지 않는 이용불능단백질 함량들을 측정하기 위하여 실시하였다. 반추위와 십이지장에 캐뉼라를 부착한 젖소에 in sacco와 mobile bag 기술을 이용하여 여러 가지 원산지와 종류가 다른 단백질 원료사료의 반추위내 분해율과 소장 내에서의 이용성을 평가한 결과는 다음과 같다. 1. 나일론백을 이용한 반추위내 단백질 분해율을 측정한 결과 UDP가에 있어 우모분(78.0), 옥수수글루텐(75.2), 어분(64.9), 알팔파 펠렛(46.6), 면실박(44.9), 채종박(42.9), 캐놀라박(41.4), 참깨박(40.6), 대두박(39.2), 해바라기씨박(30.4) 순으로 나타났다. 2. 11가지 사료를 mobile bag을 이용하여 전장 소화율을 측정한 결과 건물 소화율은 49.1%(야자박)에서 97.5%(대두박)까지, 단백질 소화율은 71.5%(우모분)에서 99.6%(대두박), 소화 가능한 UDP 함량은 63.5%(대두박)에서 99.0%(우모분)의 범위를 나타내었다. 3. 우모분, 알팔파 펠렛 및 어분 등과 같이 소화 가능한 UDP 함량이 낮은 것은 비소화성 섬유소에 결합되어 있는 ADF-N 함량도 다른 시료보다 매우 높았다. 4. 알팔파 펠렛은 지나친 열변성으로 인해 ADF-N 함량이 사료들 중에서 가장 높았으며 이로 인해 전장 단백질 소화율은 물론 소화 가능한 UDP함량도 매우 낮은 것으로 나타났다. This study was conducted to estimate the amount of rumen degradable protein(RDP), undegradable rumen dietary protein(UDP) and digestible UDP, thereby utilizing dietary protein sources in ruminant diets more effectively. Ruminally and intestinally cannulated cows were used for in sacco and mobile bag techiniques to estimate ruminal degradability and intestinal availability of protein feed ingredients. The results obtained were as follows; 1. Based on in sacco data, UDP values were ranked as feather meal(78.0), corn gluten meal(75.2), fish meal(64.9), alfalfa pellet(46.6), cottonseed meal(44.9), rapeseed meal(42.9), cannola meal(41.4), sesame oil meal(40.6), soybean meal(39.2), sunflower oil meal(30.4). 2. Actual degradabilities in total digestive tract for 11 selected feeds varied from 49.1%(palm kernel meal) to 97.5%(soybean meal) for DM and from 71.5%(feather meal) to 99.6%(soybean meal) for protein, and digestible UDP contents ranged from 63.5%(soybean meal) to 99.0%(feather meal). 3. Feather meal, alfalfa pellet and fish meal, containing low amount of digestible UDP, also have high concentrations of acid detergent fiber bound N(ADF-N) which is unavailable for animals. 4. Alfalfa pellet has highest in ADF-N concentrations among protein ingredients due to extreme heat damage, thus protein degradability in total digestive tract was also very low.

韓國 人蔘에 寄生하는 線蟲의 生物學的 防除

吳昌根 건국대학교 1980 論文集 Vol.11 No.1

In order to seek a biological control method, effective and efficient, through the way of identification of parasitic nematodes on Korean Ginseng (panax ginseng), an extensive investigation and study was n7ade !o 3,600 regions in total in the areas of Kumstn, Bu Yeo and Kimpo etc. The obtained results are summarized as follows: (1) As a natural enemy nematodes against the plants parasitic nematodes, there has been living four kinds of nematodes in the depth of 5 or 20 Cm from surface soil on order of Kimpo, Bu Yeo and Kumsan. In these areas, Aphelenchoides are found out much more than the other nematodes in classification of the enemy parasitic nematodes, and Mononchus was next to it. In the side of effectiveness, it was found omit that the treated region was revealed with a hotter result of 19.0 grams from 16.6 grams comparing the nontreated region. (2) For utilization of the enemy parasitic nematodes, a condition of the enemy parasitic nematodes living in the depth of the soil with the rate of 1:1, which was Parasitic nematodes to enemy parasitic nematodes, was the most efficient, taking consdieration upon a test of the soil. (3) In the wild sesame plants, almost none of the nematodes was living and in the case of mixed cultivation of it with Ginseng, soy bean and cotton, it leas found out not to live with plant parasitic nematodes to the extent of almost non-consideration. (4) For utilization of non-parasitic nematodes, a mixed cultivation with soy sesame and original plants (cotton and bean) in the rate more than 1 : 4 was found out to be beteer result. However, it was also found out, for Ginseng, that the first range Ginseng region only was indicated an increase of the weight with 47 percent as it's effect, comparing to parasitic nematodes region, while no efficiency from 2nd range when wild sesame was planted in the side of Ginseng region. (5) For utilization of wild sesame, an increase of the weight was found out each wish 75 percents comparing to parasitic nematodes region in the case that 2,000 grams' wild sesame dregs were fertilize per a pyung in the Ginseng region. (6) For utilization of wild sesame skin, an increase of the weight with 51.4 percents each comparing to the parasitic nematodes region, when the skin was laid on the furface of Ginseng region in width of 5 cm at the end of November and it was taken off in the month of March next year, spending a winter season. It was concluded by this studies that the plant parasitic nematodes are net only giving damages to the plants but also to be a carrier for transfering deseases by itself. Therefore, it is considered to be the most effective method for a biological control of plant parasitic nematodes comparing to chemical control, which is much expensive and supplied by only means of importing from the foreign countries.

장거리 육상선수들의 생리적 특성에 관한 고찰

오창석,이근일 龍仁大學校 1997 용인대학교 논문집 Vol.14 No.-

The purpose of this study is to analyze the statistical contribution of anthropometrical and physiological characteristics underlying 5,000 meter running performance in 5 high schools(group A) and 5 national representative(group B) male distance runners. 1.The relationship between heights(168.80cm vs 169.40cm) in 5,000 meter running performance was not significant in group A and group B, but the relationship between weights(53.80kg vs 59.20kg) in 5,000 meter running performance was significant in grouts B. 2.There were no differences in maximal heart rates(192 vs 192.40) between two groups A and B. 3.There was no significant relationship between VO2 max and 5,000 meter performance for both(76.96㎖/kg/min vs 79.80㎖/kg/min). 4.Blood lactate concentration of recovery phase was shown a significantly high lactate removal ability in group B, compared with group A(P<.02, P<.04, P<.01). 5.It was pointed out that the important causes of anthropometrical and physical attributes were ① before lactate acid ② weight ③ height (r=0.82, -0.75, 0.64) in group A. ① before lactate acid ② recovery blood lactate ③ VO2 max (r=0.75, -0.65, 0.65) in group B in the long distance running performance. As a result, it was been pointed out that a number of anthropometrical and physical attributes such as body size, body composition, running economy, pulmonary function, cardiovascular-respiratory function and running history as well as maximal oxygen consumption are relatively relevant to success in the long distance running performance. Based on the statistical analysis, high school(group A) male distance runners made more oxygen consumption and lactate than those of group B.

Ethylnitrosourea가 치배 및 치주조직에 미치는 영향에 관한 실험적 연구

오창근,조한국 慶熙大學校 齒科大學 1994 慶熙齒大論文集 Vol.16 No.1

The purpose of this study was to evaluate the effect of ethylnitrosourea(N-ethyl-N-nitrosourea ; ENU, Sigma Co.) on the tooth germs and its surrounding tissues. Forty male Wistar rats; one month of age and 100-130gm in body weight, were used in this experiment, which were devided into 3 experimental groups and 2 control groups. Ten animals of experimental group 1 were given local injection of 1% ENU solution which were dissolved in physiologic saline into the region of incisor tooth germ of the right mandible every other day for 19 days (a total of 10 times). Ten animals of experimental group 2 received ENU injections as the same manner as group 1 and were given mechanical injuries on the same regions at 2nd, 8th and 15th days after beginning of experiment. And fifteen animals of experimental group 3 received mechanical injuries as well as ENU injections as the same manner of group 2, and were given 4% ethanol as drinking water and subcutaneous injections of 2.5mg prednisolone acetate at 2 times per week. Three animals a control group 4 were treated as the same manner with experimental group 3, but received injections of physiologic saline instead of ENU, and two animals of control group 5 were not treated. All animals were sacrificed at the end of the sixth month, and the mandibles were removed, fixed in 10% N-fornialin, decalcified with electrolytic decalcification method, embedded in paraffin, sectioned in 4-6/tm thickness, and stained with Hematoxylin and eosin, and Masson's trichrome. The results were as follows ; 1. Various polycystic structures with fibrous connective tissue wall and neoplasm-like proliferation were revealed at the ENU-injected areas of the tooth germs, the periodontal ligaments and the salivary glands. 2. The epithelium of the tooth germ revealed irregular epithelial hyperplasia and disturbance of dentinogenesis. 3. The stratum intermedium of the tooth germ revealed severe adenomatous proliferations, therefore suggesting a potential of odontogenic tumors. 4. Malassez's epithelial rests in the periodontal membrane of the posterior teeth revealed mild hyperplastic proliferation. 5. Gingival hyperplasia at the posterior teeth and the formation of epithelial islands resembling with enamel organ at the affected interdental papillae were observed. 6. The ductal epithelium of the intercalated ducts and the secretory ducts of the accessory salivary glands revealed basal cell adenomatous proliferations, 7. Group 2 and 3 revealed more prominent histopathological changes than group 1, therefore it suggested that these changes would develop to odontogenic tumors.