다양한 보료를 이용한 KIOM 하수오의 포제(製) 후 각 보료별 유효성분 함량 비교

김용구,이세진,김한솔,이지혜,정유성,강영민 경상국립대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.5

Polygonum multiflorum Thunb is a perennial plant and its tubers are used as medicinal material in Korean oriental medicine. P. multiflorum is used in the treatment of various diseases. If P. multiflorum is to be used as an herbal medicine, it is necessary to promote active ingredients or reduce the toxicity of plants, which requires processing methods. In this study, the content of active compound was verified through the treatment of various supplementary materials in the processing method of P. multiflorum and an experiment was conducted to verify the supplement suitable for the P. multiflorum processing method. The P. multiflorum used in the study used two types of P. multiflorum. The P. multiflorum of distribution products and KIOM's Polygonum multiflorum Thunb system developed through tissue culture research base on KIOM patents were used. The content of emodin in P. multiflorum was measured high in all (Black soybean juice, Rice water, Licorice and soybean juice,Rosemary extract) but the treatment area using sesame oil. In particular, KIOM's was found to contain higher emodin content (0.29 ug/mg) in Licorice and soybean juice than previously known Polygonum multiflorum Thunb supplement, black soybean juice. The results of this study are believed to be used as basic knowledge for standardization and modernization in P. multiflorum processing methods. 하수오(Polygonum multiflorum Thunb)는 다년생 덩굴성 식물로, 지하부인 뿌리(tuber)는 한의학에서 약재로 다양한 질병의 치료에 사용되고 있다. 하수오를 한약재로 사용하기 위하여 약용물질의 증진 또는 식물의 독성을 줄이기 위해 1차 가공 및 포제를 한다. 본 연구에서는 하수오 포제에있어 다양한 보료처리를 통해 주요물질의 유효성분 함량을 확인 해 보고, 하수오 포제에 적합한 보료를 검증하는 실험을 진행하였다. 또한, 실험에두 가지 타입의 하수오를 활용하여 기존 유통품 하수오와 조직배양 연구를 통해 개발된 KIOM 특허 기반 하수오를 비교하는 실험을 진행하였다. 하수오의 주요 지표물질인 emodin의 함량이 참깨추출물을 보료로 사용한 처리구를 제외한 나머지 보료 처리구(흑두즙, 쌀뜨물, 감두즙, 로즈마리추출물)에서 KIOM품이 유통품보다 높게 측정되는 것을 확인하였다. 특히 KIOM품은 기존에 알려진 하수오 보료인 흑두즙보다 감두즙을 보료로 사용해포제 하였을 때 0.29 ug/mg 더 높은 emodin의 함량이 확인 되었다. 본 연구결과를 통해 하수오의 약용자원으로 사용함에 있어 앞으로 KIOM하수오의생산과 포제에 관련된 연구가 더 이루어 져야 할 것이며, 연구 결과는 하수오의 포제에 있어 표준화와 현대화를 위한 기초 지식 및 결과로 사용될 것이라고 사료된다.

Nuclear Factor Kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of <i>Polygonum multiflorum</i>

Sun, Ya Nan,Li, Wei,Song, Seok Bean,Yan, Xi Tao,Yang, Seo Young,Kim, Young Ho Medknow PublicationsMedia Pvt Ltd 2016 Pharmacognosy magazine Vol.12 No.45

<P><B>Background:</B></P><P><I>Polygonum multiflorum</I> is well-known as “Heshouwu” in traditional Chinese herbal medicine. In Northeast Asia, it is often used as a tonic to prevent premature aging of the kidney and liver, tendons, and bones and strengthening of the lower back and knees.</P><P><B>Objective:</B></P><P>To research the anti-inflammatory activities of components from <I>P. multiflorum</I>.</P><P><B>Materials and Methods:</B></P><P>The compounds were isolated by a combination of silica gel and YMC R-18 column chromatography, and their structures were identified by analysis of spectroscopic data (1D, 2D-nuclear magnetic resonance, and mass spectrometry). The anti-inflammatory activities of the isolated compounds 1−15 were evaluated by luciferase reporter gene assays.</P><P><B>Results:</B></P><P>Fifteen compounds (1–15) were isolated from the roots of <I>P. multiflorum</I>. Compounds 1−5 and 14−15 significantly inhibited tumor necrosis factor-α-induced nuclear factor kappa B-luciferase activity, with IC<SUB>50</SUB> values of 24.16-37.56 μM. Compounds 1−5 also greatly enhanced peroxisome proliferator-activated receptors transcriptional activity with EC<SUB>50</SUB> values of 18.26−31.45 μM.</P><P><B>Conclusion:</B></P><P>The anthraquinone derivatives were the active components from the roots of <I>P. multiflorum</I> as an inhibitor on inflammation-related factors in human hepatoma cells. Therefore, we suggest that the roots of <I>P. multiflorum</I> can be used to treat natural inflammatory diseases.</P><P><B>SUMMARY</B></P><P><P>This study presented that fifteen compounds (1-15) isolated from the roots of Polygonum multiflrum exert signifiant anti inflmmatory effects by inhibiting TNF α induced NF κB activation and PPARs transcription.</P></P> >[FIG OMISSION]</BR><P>Abbreviation used: NF κB: Nuclear factor kappa B, PPARs: Peroxisome proliferator activated receptors, PPREs: Peroxisome proliferator response elements, TNF α: Tumor necrosis factor α, ESI-MS: Electrospray ionization mass spectrometry, HepG2: Human hepatoma cells</P>

Anti-inflammatory effects of novel <i>polygonum multiflorum</i> compound via inhibiting NF-κB/MAPK and upregulating the Nrf2 pathways in LPS-stimulated microglia

Park, Sun Young,Jin, Mei Ling,Kang, Nam Jun,Park, Geuntae,Choi, Young-Whan Elsevier/North-Holland 2017 Neuroscience letters Vol.651 No.-

<P><B>Abstract</B></P> <P>The incorporation of <I>Polygonum multiflorum</I> into the diet can result in anti-aging effects owing to its wide range of biological and pharmaceutical properties. We investigated the anti-neuroinflammatory properties of CRPE56IGIH isolated from <I>P. multiflorum</I> by focusing on its role in the induction of phase II antioxidant enzymes and the modulation of upstream signaling pathways. In microglia, CRPE56IGIH significantly inhibited lipopolysaccharide (LPS)-stimulated nitric oxide and prostaglandin E<SUB>2</SUB> production with nonspecific cytotoxicity. CRPE56IGIH also markedly inhibited LPS-inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 protein and mRNA expression in the same manner as it inhibited nitric oxide and prostaglandin E<SUB>2</SUB> production. In the control cells, NF-κB transactivation and nuclear translocation occurred at a baseline level, which was significantly increased in response to LPS. However, pretreatment with CRPE56IGIH concentration-dependently inhibited the LPS-induced NF-κB transactivation and nuclear translocation. The phosphorylation of Janus kinase-signal transducers and activators of transcription and mitogen-activated protein kinases was markedly upregulated by LPS, but considerably and dose-dependently inhibited by pretreatment with CRPE56IGIH. Furthermore, CRPE56IGIH induced the expression of phase II antioxidant enzymes, including heme oxygenase-1 (HO-1) and NADPH dehydrogenase quinone-1 (NQO-1). The activation of upstream signaling pathways, such as the Nrf2 pathway, was significantly increased following CRPE56IGIH treatment. Furthermore, the anti-neuroinflammatory effect of CRPE56IGIH was reversed by transfection of Nrf2, HO-1, and NQO-1 siRNA. Our results indicated that CRPE56IGIH isolated from <I>P. multiflorum</I> could be used as a natural anti-neuroinflammatory agent that induces phase II antioxidant enzymes <I>via</I> Nrf2 signaling.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CRPE56IGIH is a natural compound isolated from <I>Polygonum multiflorum.</I>. </LI> <LI> CRPE56IGIH inhibits LPS induced neuroinflammatory response in microglia. </LI> <LI> CRPE56IGIH inhibits LPS-induced NF-κB and JAK-STATs activation in microglia. </LI> <LI> Nrf2 mediates the anti-neuroinflammation of CRPE56IGIH in microglia. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Inhibitory Effects of Stilbene Glucoside Isolated from the Root of Polygonum multiflorum on Tyrosinase Activity and Melanin Biosynthesis

( Ju Kim ),( Jung Hoon Kim ),( Eun Sun Jeung ),( In Sun Park ),( Chung Hyeon Choe ),( Tae Ho Kwon ),( Kang YeoI Yu ),( Seung Il Jeong ) 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.4

As part of efforts to discover phytochemicals that inhibit melanin biosynthesis, we screened (E)-2,3,5,4`-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) isolated from Polygonum multiflorum. THSG dose-dependently inhibited tyrosinase activity, with an ID50 value of 100 ㎍/mL. THSG also potently inhibited melanin production in B16 melanoma cells induced by forskolin, an inducer of melanogenesis, with ID50 of 1-10 ㎍/mL. THSG may therefore reduce melanogenesis.

하수오의 우량종묘 생산을 위한 담액수경(湛液水耕) 육묘시 종묘 크기와 양액의 종류가 생육에 미치는 영향

이가연,민지윤,최지은,문병철,강영민 경상대학교 농업생명과학연구원 2017 농업생명과학연구 Vol.51 No.1

하수오(Polygonum multiflorum Thunb.)는 여뀌과에 속하는 초본식물로 동아시아와 북아메리카에 자생하고 있다. 하수오는 관상용으로도 이용되며, 한의학에서 뿌리를 약용으로 사용한다. 하지만 월동 이 어렵고 노동력 소모가 많아 최근 재배농가가 감소하고 있다. 본 연구에서는 하수오의 우량종묘를 짧 은 시간에 대량생산하기 위하여 수경재배에 적합한 양액과 조직배양묘의 크기를 조사하였다. 토양에 적 응이 완료된 하수오 종묘는 크기에 따라 Type 1, Type 2 및 Type 3으로 나누어 4종류의 양액에 치상 하였다. 치상 후 양액과 종묘에 따른 생육차이를 확인하기 위하여 생육조사를 실시하였다. 생육조사는 8주 동안 양액에서 생육시킨 후 줄기길이, 잎 수, 마른 잎 수, 줄기 수, 생체중과 건물중에 대해 측정 하였다. 그 결과 양액3에서 Type 1과 Type 2의 뿌리길이는 각각 33.8cm와 29.5cm로 각각 나타났다. 이와 유사한 경향으로 덩굴 길이도 CaCl2가 첨가 된 양액3에서 Type 1과 Type 2가 가장 길게 나타나 우수한 생육을 보였다. 이처럼 뿌리가 증대된 종묘는 밭으로 이식 될 때 활착을 도와 우수한 초기생육 을 나타낼 것이라 사료된다. 반면 Type 3는 잎끝마름과 고사하는 현상을 보여 추후 양액의 적정농도에 대한 추가 실험이 필요할 것이라 사료된다. Polygonum multiflorum Thunb. is a herbaceous plant of Polygonaceae and distribution on East Asia and North America. P. multiflorum is used as horticultural crops and root is used as a traditional herbal medicine. But recently, farmhouse is reduced because difficult overwintering and a lot of labors are required. In this study, we have focused on investigation of suitable to seedling size and nutrient solution in deep flow technique system for mass production good seedling of P. multiflorum. The environmental acclimatization seedling was classified as Type 1, Type 2, and Type 3 according to the size with sowing in four nutrient solution. Growth experiment was carried out to confirm the growth differences according to the size of seedling and nutrient solutions. Growth experiment was measuring the stem length, number of leaves, number of dead leaves, number of stem, fresh weight, and dry weight growing after 8 weeks. As a results, root length of Type 1 and Type 2 were 33.8 cm and 29.5 cm at NS3, respectively. Similarly, vine length of Type 1 and Type 2 were best grown up from the NS3 with CaCl2. These results were considered that seedling of enlarged root was effective on the early stage of growth by helping the rooting in the field. On the other hand, it was happened as tip burn and withered on leaf in Type 3 case and it might be required additional experiment for the optimal concentration of the nutrient solution.

표고균사체 발효하수오 배양적 특성 및 이화학적 성분

오준석,홍재희,박태영,김경제,진성우,반승언,고영우,정상욱,임승빈,서경순 한국버섯학회 2016 한국버섯학회지 Vol.14 No.4

This study was performed to determine the optimal condition for Lentinula edodes JMI-10079 mycelium cultivation on the root of Polygonum multiflorum. We also analyzed the proximate composition, total amino acids, and minerals in the root of P. multiflorum cultivated with L. edodes JMI-10079 mycelia. The optimal temperature and pH for L. edodes JMI-10079 mycelium cultivation on the P. multiflorum root were 25oC and pH 5–6 respectively, whereas the optimal carbon and nitrogen sources were glucose and maltose, respectively. The content of crude protein, crude fat, and ash in the P. multiflorum root cultivated with L. edodes JMI-10079 mycelia was higher than that in the uncultivated P. multiflorum root. The content of crude fiber was the highest in the control. Total amino acid analysis revealed that the contents of total amino acids and total essential amino acids were increased by higher root of P. multiflorum concentration.

Drug-Induced Liver Injury: Twenty Five Cases of Acute Hepatitis Following Ingestion of <i>Polygonum multiflorum</i> Thunb

Jung, Kyoung Ah,Min, Hyun Ju,Yoo, Seung Suk,Kim, Hong Jun,Choi, Su Nyoung,Ha, Chang Yoon,Kim, Hyun Jin,Kim, Tae Hyo,Jung, Woon Tae,Lee, Ok Jae,Lee, Jong Sil,Shim, Sang Goon The Korean Society of Gastroenterology; the Korean 2011 Gut and Liver Vol.5 No.4

<P><B>Background/Aims</B></P><P>Complementary medicines, including herbal preparations and nutritional supplements, are widely used without prescriptions. As a result, there has been growing interest in the risk of hepatotoxicity with these agents. It is difficult to determine causal relationships between these herbal preparations and hepatotoxicity. We report on 25 patients diagnosed with toxic hepatitis following ingestion of <I>Polygonum multiflorum</I> Thunb.</P><P><B>Methods</B></P><P>Twenty-five patients (median age, 48 years [24 to 65 years]; M:F=18:7) with suspected <I>P. multiflorum</I> Thunb-induced liver injury were admitted to our hospital between 2007 and 2009. We analyzed clinical and histological data, including the types and the duration of <I>P. multiflorum</I> Thunb intake and the duration of hospital care. We also determined the type of liver injury using the R ratio (serum activity of ALT/serum activity of ALP).</P><P><B>Results</B></P><P>The types of complementary medicine used included tea (n=16), liquor (n=5), tea and liquor (n=2), powder (n=1), and honeyed pudding (n=1). The most common presenting sign was jaundice (76%), and 18 patients (72%) had evidence of hepatocellular liver injury. Histological findings were consistent with acute hepatitis in all cases (n=10) for which liver biopsy was performed. Twenty-three patients (91.6%) recovered with conservative management, 1 patient (4%) had a liver transplant, and 1 patient (4%) died of hepatic failure.</P><P><B>Conclusions</B></P><P>In our cases, we found that <I>P. multiflorum</I> Thunb could be hepatotoxic and could lead to severe drug-induced liver injury, and even death.</P>

백수오, 이엽우피소, 박주가리 및 하수오의 형태적 형질 비교

김민자,김인재,최성열,한동호,김영호,임상철,김태중,남상영,송범헌,오병운,박춘근 韓國藥用作物學會 2014 한국약용작물학회지 Vol.22 No.2

These studies were conducted to discriminate Cynanchum wilfordii, C. auriculatum, Metaplexis japonica, andPolygonum multiflorum by morphological characters. Cynanchum wilfordii, C. auriculatum, and Metaplexis japonica(Ascle-piadaceae) were easily distinguished from Polygonum multiflorum by twining stem to the left, producing opposite leaves,having laticiferous stems and leaves at cutting cross-sectionally, and lacking lignification of the stem. Four species had differ-ent morphological characters of flower. Comparing four species to fruit type, three species of Asclepiadaceae had follicle,while Polygonum multiflorum had achene with three wings. Follicle shape was lanceolate, narrowly lanceolate, and widelylanceolate in C. wilfordii, C. auriculatum, and Metaplexis japonica, respectively. Whereas seed color of four species was darkbrown similarly, seed shape and 1,000 seed weight were useful characters to discrimate between three species ofAsclepiadaceae and Polygonum multiflorum. Shape and color of root were thickened and yellowish brown in C. wilfordii andC. auriculatum. They were thin and long and white in Metaplexis japonica, and fusiform and reddish brown in Polygonummultiflorum. They would be useful characters to distinguish Cynanchum spp., Metaplexis japonica, and Polygonummultiflorum, however, impossible characters to discriminate between C. wilfordii and C. auriculatum.

Inhibitory Effects of Stilbene Glucoside Isolated from the Root of Polygonum multiflorum on Tyrosinase Activity and Melanin Biosynthesis

Kim, Ju,Kim, Jung-Hoon,Jeung, Eun-Sun,Park, In-Sun,Choe, Chung-Hyeon,Kwon, Tae-Ho,Yu, Kang-Yeol,Jeong, Seung-Il The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.4

As part of efforts to discover phytochemicals that inhibit melanin biosynthesis, we screened (E)-2,3,5,4'-tetrahydroxystilbene-2-O-$\beta$-D-glucoside (THSG) isolated from Polygonum multiflorum. THSG dose-dependently inhibited tyrosinase activity, with an $ID_{50}$ value of $100{\mu}g$/mL. THSG also potently inhibited melanin production in B16 melanoma cells induced by forskolin, an inducer of melanogenesis, with $ID_{50}$ of 1-$10{\mu}g$/mL. THSG may therefore reduce melanogenesis.

생물반응기를 이용한 적하수오 부정근의 바이오매스와 생리활성물질 대량생산

이경주,박영기,김자영,정택규,윤경섭,백기엽,박소영 한국식물생명공학회 2015 식물생명공학회지 Vol.42 No.1

This study was conducted to investigate theproductivity of biomass and antioxidant compounds in Polygonummultiflorum by culturing explants in air-lift bioreactor containingMurashige and Skoog (MS) medium, by adding differentconcentrations of auxins [indole-3-butyric acid (IBA) andnaphthalene acetic acid (NAA)], sucrose, methyl jasmonate(MeJA), and salicylic acid (SA). Results of this studyrevealed that the explants culturing on the medium supplementedwith 9.84 μM IBA and 50 g/L sucrose wereobserved to have higher productivity of biomass and bioactivecompound than other treatments used. Thus, we expect thatthese results will be helpful for large-scale production ofbiomass and antioxidant compounds from Polygonum multiflorum.