Korean Red ginseng prevents endothelial senescence by downregulating the HO-1/NF-κB/miRNA-155-5p/eNOS pathway

Tae-Hoon Kim,Ji-Yoon Kim,Jieun Bae,Young-Mi Kim,Moo-Ho Won,Kwon-Soo Ha,Young-Guen Kwon,Young-Myeong Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2

Background: Korean Red ginseng extract (KRGE) has beneficial effects on the cardiovascular system by improving endothelial cell function. However, its pharmacological effect on endothelial cell senescence has not been clearly elucidated. Therefore, we examined the effect and molecular mechanism of KRGE on the senescence of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were grown in normal or KRGE-supplemented medium. Furthermore, they were transfected with heme oxygenase-1 (HO-1) gene or treated with its inhibitor, a NF-κB inhibitor, and a miR-155-5p mimic or inhibitor. Senescence-associated characteristics of endothelial cells were determined by biochemical and immunohistochemical analyses. Results: Treatment of HUVECs with KRGE resulted in delayed onset and progression of senescence-associated characteristics, such as increased lysosomal acidic β-galactosidase and decreased telomerase activity, angiogenic dysfunction, and abnormal cell morphology. KRGE preserved the levels of anti-senescent factors, such as eNOS-derived NO, MnSOD, and cyclins D and A: however, it decreased the levels of senescence-promoting factors, such as ROS, activated NF-κB, endothelial cell inflammation, and p21 expression. The beneficial effects of KRGE were due to the induction of HO-1 and the inhibition of NF-κB-dependent biogenesis of miR-155-5p that led to the downregulation of eNOS. Moreover, treatment with inhibitors of HO-1, NF-κB, and miR-155-5p abolished the anti-senescence effects of KRGE. Conclusion: KRGE delayed or prevented HUVEC senescence through a signaling cascade involving the induction of HO-1, the inhibition of NF-κB-dependent miR-155-5p biogenesis, and the maintenance of the eNOS/NO axis activity, suggesting that it may protect against vascular diseases associated with endothelial senescence.

역행적 골수정 삽입술을 이용한 대퇴골 과상부 골절의 치료

권진우,신승호,조원호,성기호,김기환,손경태,이상훈,박세원,배종문,권태우 대한골절학회 2003 대한골절학회지 Vol.16 No.4

Synthesis of Sr Doped HoCrO3 Solid Solution Systems and Their Electrical Properties

PARK, SUNG HO,Kwon, Tae Yun 한국공업화학회 2000 Journal of Industrial and Engineering Chemistry Vol.6 No.4

Ho_(1-x)SrxCrO₃ systems, with perovskite structures, were synthesized for x=0, 0.05, 0.1, 0.2 at 1300 ℃ under atmospheric pressure. The results of X-ray analysis reveals that these prepared systems forms orthorhombic structures. Also, the HoCrO₃ systems which were doped with Sr cations on the site of Ho cations, exhibited a linear increase in their cell volume. It was found that the lattice parameter and reduced volume increased as the doping level of Sr^(2+) increased. From the measurements of D.C conductivity, the activation energy obtained from the conductivity dependence on temperature was 0.18 eV. The conduction mechanism would appear to be the hopping model of electron conduction.

CAD를 이용한 양수장 및 배수장설비 설계의 표준화

권병철,권경우,김기길,명태식,박찬호,박귀용 한밭대학교 산업과학기술연구소 1995 논문집 Vol.3 No.-

This paper is to be illustrated automation in the design of Pumping Station and Draining Station. Program for design automation applying Auto LISP, DCL can be used to design and draw simultaneously. The study result are as follows. 1. Optimal design has been introduced and precise design has been possible using CAD. 2. Dialog design is possible with introduction the concept of design automation, and design cost can be reduced greatly.

접착제 미 사용시 치면열구전색제로서의 유동성 복합레진 평가

권호범,김명진,신철환,김지연,박기태 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

본 연구의 목적은 유동성 복합레진을 치면열구전색제로 사용할 수 있는지 여부를 평가하는 것이다. 세 종류의 유동성 복합레진(Filtek Flow, Tetric Flow, Charmfil Flow)과 한 종류의 filled sealant(Ultraseal XT plus)가 사용되었다. 주사전자 현미경을 이용하여 세 종류의 유동성 복합레진 및 filled sealant의 resin tag 형성 양상을 비교하였다. 미세누출 평가를 위해 발거된 사람의 소구치 54개를 각각 18개씩 무작위로 세 군으로 배분하였다. 각 군마다 통상의 filled sealant 와 한 가지씩의 유동성 복합레진을 교합면 열구에 도포하였다. 치아들은 thermocycling(5˚ ± 2℃와 55˚ ± 2℃ 사이에서 30초씩 1200회 시행) 후 48시간 동안 1% methylene blue 용액에 보관하였다. 각각의 치아를 절단하여 미세누출 정도를 관찰하였다. 세 종류의 유동성 복합레진 및 filled sealant는 유사한 resin tag 형성양상을 보였다. 세 종류의 유동성 복합레진은 각각 filled sealant보다 현저히 더 많은 미세누출을 보였다. 세 종류의 유동성 복합레진 간의 미세누출 정도는 유사하였다. 유동성 복합레진은 통상의 filled sealant와 비교해볼 때 보다 많은 미세누출을 야기할 수 있으므로 치면열구전색제로 적절하지 않다. The aims of this study were to determine if flowable composites can be used as pit and fissure sealants without bonding agents. Three flowable composites(Filtek Flow, Tetric Flow, Charmfil Flow) and a filled sealant (Ultraseal XT plus) were used. The pattern of resin tag formation in the four sealant meterials were compared using SEM. For the microleakage assessment, 54 extracted human premolar teeth were randomly divided into 3 groups containing 18 premolars each. In each group, a conventional filled sealant and one of the three flowable composites were applied to occlusal fissures. The teeth were thermocycled(1200 cycles between 5˚± 2℃ and 55˚± 2℃ with a dwell time of 30 seconds) and immersed in a 1% methylene blue solution for 48 hours. Each tooth was sectioned and examined to determine the extent of dye penetration. Three flowable composites and a filled sealant showed a similar resin tag formation pattern. The three flowable composites showed significantly more microleakage in each group than the filled sealant. The level of microleakage was similar in the three flowable composites. Flowable composites are not recommended as pit and fissure sealants because more microleakage can occur even when occlural fissures are mechanically widened.

마이크로프로세서를 利用한 溫度制御

權泰夏,鄭信一,禹鐘鎬 釜山水産大學校 1982 釜山水産大學 硏究報告 Vol.22 No.2

本 論文에서는 마이크로프로세서와 溫度符號化器를 利用해서 溫度를 計數的으로 制御할 수 있는 시스템을 보다 간단히 構成하였으며 上昇기울기, 維持溫度, 降下기울기에 따라 多少의 差는 있으나 制御溫度의 變化幅이 ±0.3% 以內로 比較的 良好하게 制御되었다. bit數가 많은 A/D 變換器를 使用한다면 보다 더 精密한 溫度制御가 可能할 것이다. 現在狀態로도 實驗室의 小容量 뿐 만 아니라 産業用의 大容量 電氣爐의 溫度制御에도 利用될 수 있을 것으로 본다. This paper describes the temperature control system of an electric furnace using a SDK-85 microprocessor system as a control unit. The hardware structure of the control system was very simplified by eliminating mechanical drive portions and employing electronic circuits instead. Moreover, by employing a software method in the system desired temperature on time, i.e., rising rate, maintaining temperature and time, falling rate, can be easily controlled and changed by program. Experimental results showed the successful operation with flexibility. Temperature variation was less than ±0.3% of the desired value and was more accurate on the maintaining temperature region.

이동통신망에서의 최적 셀 할당에 관한 연구

권수태,정소영,윤상흠,김호준 전주대학교 공학기술종합연구소 2003 전주대학교 공학기술종합연구소 학술논문집 Vol.9 No.1

The paper considers a cell allocation problem in the Mobile Wireless Communication Network with limited capacity of Base Switching Center(BSC) and Mobile Switching Center(MSC), and the aim is assigning cells to BSC and MSC for supporting an efficient handoff and reducing the cabling cost. The cell assignment problem is formulated as a mixed integer programming, and a genetic algorithm is developed to derive the optimal solution. A verity of numerical problems are presented and then some interesting properties are exploited that are useful for charactering the problem.

付審判節次에 關한 考察

權泰鎬 청주대학교 대학원 1989 우암논총 Vol.5 No.-

As to the application by the accuser who has objected to the disposition not to institure a public prosecution by the public prosecutor, the High Court shall rule or whether the dispositions are proper or not. This system has been derived from the system of compulsory indictment of West Germany. The West Germany compulsory indictment system is under purpose to secure the legal public prosecution of the case and all the crimes are to be the object thereof and that it shall be ordered to institute public prosecution. Our system however, in contrary to West Germany system, has the function restricting the discretionary indistment like Japan and public prosecution shall be deemed to have been instituted in the case. The claimant is an accuser who has been noticed for the disposition not to institute public prosecution from the public prosecutor. The case eligible for an application shall be limited to the climes under Article 123 to Article 125 of the Criminal Code. An application shall be filed to the High Court which corresponds to the Higher Prosecutors' Office to which the public prosecutor belongs through the Chief Prosecutor of a district Prosecutors' Office to which the public prosecutor who has disposed not to institute Public prosecution in writting within 10 days from the date of receiving the notice of that disposition. If the applcation is reasonable, the High Court shall commit the case to the competent district court for trial. And, if such an application is not reasonable, the High Court shall dismiss it. The case as has been committed to the competent district court for trial shall be applied its maintenance of public prosecution by the advocate who has been disignated by the Court as the person in charge for maintenance of the public prosecution not by the public prosecutor. The advocate appointed as maintenance person of the public prosecution shall exercise, ex officio, the duties of a public prosector in order to maintain public prosecution until the Judgement is finally settled. As a legislative theory of this system I propose the following. 1. to expand objects for this system 2. to effectively connect with the public prosecutors' appeal system, and 3. to revise the procedure so that may meet with the spirit of filing only the written indictment.

Allylisothiocyanate에 依한 窒酸鹽還元酵素의 不活性化

權泰英,朴聖鎬 Natural Science Research Institute JEONJU UNIVERSI 1990 전주대학교 자연과학연구소 학술논문집 Vol.3 No.1

Inhibitory effect of glucosinolate decomposites and related substances, such as allyl-NCS. allyl-SCN, allyl-CN, allylthiourea, N'-dimethylallylthiourea. 3-butenyl-SCN, 4-pentenyl-SCN, 2-phenylethyl-SCN, thiourea, sodium thiocyanate and potassium cyanide, on the activity of nitrate reductase preparation from spinach were studied at 27℃ and pH7.8. All tested compounds, except allyl-CN, inhibited nitrate reductase at below 0.1∼1.0mM level, and ineffective on nitrate and nitrite under this experimental condition. Inhibition by allyl-NCS was non-competitive and Ki value 1.20mM. Ethanol commonly detected compound in fermented foods strongly inhibited at 1.0% (v/v_o).

동측에 발생한 대퇴골 경부 및 간부 골절의 치료

권진우,김기환,문상호,공규민,손경태,이상훈,권태우 대한골절학회 2003 대한골절학회지 Vol.16 No.3

목적: 동측의 대퇴골 경부 골절과 간부 골절이 동반된 경우에서 수술적 치료에 대해 본원에서 실시한 수술 방법들의 결과를 분석하였다. 대상 및 방법: 수술 후 1년 이상 추시 관찰이 가능하였던 11례를 대상으로 하였으며 경부 골절은 Garden 제 1형이 3례, 제 2형이 6례, 제 3형이 1례, 제 4형이 1례였다. 간부 골절은 중간부가 7례, 원위부가 4례였다. 추시 기간은 최소 1년 이상으로 평균 33개월이었고 11례 전부가 남자였으며 나이는 22세에서 54세로 평균 38세였다. 각 수술 방법에 따른 수술시간을 측정하였으며 방사선학으로 골유합의 시기, 부정유합, 불유합 등을 평가하였고 임상적으로는 골절부의 동통 및 관절 운동장애를 평가하였다. 결과: 치료 방법으로는 경부는 다발성 피고정술이 8례, 활강압방 고 나사못고정술이 1례였으며 간부의 경우는 중간부는 역행적 Ender정 고정술이 4례 , 금속판 고정술이 1례, 재건상 골수강내 고정술이 2례, 역행적 금속정 고정술이 1례였고, 원위부는 금속판 고정술이 1례, 역행적 교합성 금속정 고정술이 3례였다. 평균 수술시간은 재건상 골수강내 골종술이 180분, 다발성 핀고정술 및 금속판 고정술이 220분, 다발성 핀고정술 및 역행적 골수강내 고정술이 200분이었다. 골유합 기간은 간부 골절의 경우 평균 5개월이었다. 경부 골절에 대한 합병증으로는 간부 골절에 대해서 Ender정 고정술을 한 1례와 간부에 금속판 고정술을 한 후 지연진단되어 경부에 다발성 핀고정술을 한 1례에서 대퇴골두에 무혈성 괴사증이 병발하였으며 재건상 골수강내 금속정 삽입술을 한 1례에서 수술시 경부 골절의 정복소실이 있었으며 간부 골절에 대한 합병증으로는 역행적 금속정 고정술을 한 2례 (Ender정 1례와 교합성 금속정 1례)에서 불유합이 생겨서 금속판 고정술 및 자가골이식술로 대치하였다. 그리고 Ender정 고정술을 한 1례에서 지속적인 슬관절 주위 동통이 있었다. 결론: 대퇴골 경부 골절과 동측의 간부 골절이 동반된 경우의 수술적 치료에서 재건상 골수강내 금속 고정술은 기술적으로 어려움이 많아서 경부와 간부의 치료를 각각 하는 것이 기술적으로 쉬웠다. 수술시는 경부 골절의 수술을 먼저 하여도 고정에 어려움이 없었으며 간부 골절은 분쇄 골절이 많기 때문에 견고한 내고정이 필요하였다. Purpose: The purpose of this study was to analysis the result of operative treatment in ipsilateral femur neck and shaft fracuture. Material and Methods: Eleven cases of ipsilateral femur neck and shaft fractures were operated and followed more than 1 year. There were 11 men and mean age at index operation was 38 years (range 22~54). In neck fracture there were 3 of type Ⅰ, 6 of type Ⅱ, and 1 of typeⅢ according to Garden classification, shaft fractures were located an middle 1/3 (7 cases), at distal 1/3 (4 cases). We evaluated the operation time, union time, nonunion, malunion, and clinical evaluation with pain and joint stiffness. Result: In neck fractures treatment methods were 8 of multiple pinning, 2 of reconstruction nail and 1 of CHS, in shaft fractures 2 of DCP, 7 of retrograde nailing (3 Ender nail, 4 interlocking nail). The mean operation time was 180 minutes in reconstruction nail, 220 minutes in multiple pinning and DCP and 200 minutes in multiple pinning and retrograde nailing. The mean time for shaft union was 5 months. The complications were 1 case of neck reduction loss during operation in reconstruction nailing group, 1 of persistant pain around knee in Ender nailing group. Conclusion: The author think that reconstruction nail fixation is technically difficult, thus neck and shaft fracture be fixed individually. Neck fracture can be fixed first without difficulty and shaft fractures should be fixed rigidly due to comminution.