http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
ITS2 DNA 바코드 염기서열 분석 기반 독활 신종 위품 유통사례 보고
문병철,김욱진,최고야 한약정보연구회 2017 한약정보연구회지 Vol.5 No.1
Araliae Continentalis Radix has been known as a representative herbal medicine distributed with inauthentic adulterants such as the roots of Angelica biserrata and Levisticum officinale. In this study, we identified a new additional commercial adulterant used as Araliae Continentalis Radix. To define the taxonomic origin of the new commercial adulterant, we analyzed ITS2 sequence similarity and identity using NCBI BLAST. The BLAST analysis revealed that the new commercial adulterant belong to the genus Gypsophila but not identical to any plant species. To verify the accurate species, we compared ITS2 sequences of the new commercial adulterant and related taxa including Ar. continentalis, An. biserrata, L. officinale, G. oldhaniana, G. pacifica, and other Gypsophila species and confirmed the new commercial adulterant was the root of G. facifica. These results provide an useful information to prevent the adulteration of Araliae Continentalis Radix and suggest an effective method to verify the origin of commercial herbal medicines.
RAPD 분석과 multiplex-PCR을 이용한 석창포 감별용 SCAR 마커 개발
문병철,지윤의,이영미,천진미,이아영,추병길,김호경 韓國藥用作物學會 2011 한국약용작물학회지 Vol.19 No.3
The rhizomes and herbal medicines originating from Acorus gramineus, A. calamus, A. tatarinowii, and A. gramineus var. pusilus, show significant similarity, and the correct identification of species is very difficult. Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop a reliable method for identification of these four species. Several distinct SCAR markers were developed from species-specific RAPD amplicons for each species. Furthermore, a useful molecular marker was established for multiplex-PCR, in order to the four species could be distinguished concurrently. These markers allow efficient and rapid identification of closely-related Acorus species and will be useful for standardization of herbal medicines.
The potential of earthworm and its components as a therapeutic agent for neuronal damage
문병철,김중선 충북대학교 동물의학연구소 2018 Journal of Biomedical and Translational Research Vol.19 No.3
Earthworms have been employed in traditional oriental medicine for thetreatment of neurological disorders, as anticonvulsants, analgesics, and sedatives. In fact, earthworms are currently used as a medicinal agent in China, South Korea, Japan, Taiwan and North Korea. However, both the species and the genus of the earthworms registered in the pharmacopoeia of each country are different. Neural injury is induced by oxidative stress, inflammation, and apoptosis. The role of various synthetic chemicals of earthworms as antioxidant and anti-inflammatory agents have been studied and earthworm extract and its components have been shown to protect nerve cells and restore nerve function in various preclinical neuronal damage models. We employed earthworm extracts to provide prevention and treatment strategies for many neurodegenerative disorders including Parkinson's disease, mild cognitive impairment, cerebral infarction, and peripheral nerve damage. In this study, we investigated the effects of earthworm extracts and its components to explore their prophylactic and therapeutic effects in various neuropathic models. We used earthworm resources to provide prevention and treatment strategies for many neurodegenerative disorders including Parkinson's disease, mild cognitive impairment, cerebral infarction,and peripheral nerve damage. We summarized the protective effects of both earthworms and their extracts on neuropathies. The current study identified some earthworm components to be used in treatment and prevention strategies for nerve disorders and could be helpful for the development of new therapies for intractable diseases.
psbA-trn 바코드 H DNA 분석을 통한 후박 및 토후박 감별
문병철 한약정보연구회 2014 한약정보연구회지 Vol.2 No.2
The original plant species of Magnoliae Cortex (Hu-Bak in Korean and Hòu-Pò in Chinese) is prescribed as the stem bark of Magnolia officinalis Rehder & E.H.Wilson, Magnolia officinalis var. biloba Rehder & E.H.Wilson, and Magnolia ovobata Thunb. However, the stem bark of Machilus thunbergii Siebold & Zucc. also has been used as a domestic Magnoliae Cortex, To-Hu-Bak. To prevent the distribution of inauthentic Machilus Cortex, we introduced psbA-trnH DNA barcode analysis and established a reliable method for distinguishing between Manoliae Cortex and Machilus Cortex. In comparative analysis of the psbA-trnH sequences using 18 samples of four species, thus, we obtained distinct nucleotide sequences, such as distinct indels (insertions and deletions of nucleotide) and substitutions, enough to differentiate these two herbal materials and evaluated the phylogenetic relationship of these species. The sequence differences at the corresponding alignment positions are useful marker nucleotides to authenticate official Magnoliae Cortex from closely related adulterant of Machilus thunbergii. These marker nucleotides would be useful to standardize the Magnoliae Cortex by the providing of specific genetic information that can distinguish official herbal medicine from inauthentic adulterants for Hu-Bak.
문병철,박인규,김욱진,양선규,강영민 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.1
The genus Fritillaria is an important herbaceous medicinal plant. The dried bulbs of many Fritillaria species are used asFritillariae Bulbus in traditional oriental medicines. However, these herbal medicines frequently contain mixtures of differenttypes of Fritillariae Bulbus and other Fritillaria species with little or no medicinal value. The accurate identification ofdifferent types of Fritillariae Bulbus is very difficult due to the morphological similarities between the plants and dried bulbs;therefore, it is necessary to accurately identify these herbal medicines and other Fritillaria species based on their geneticcharacteristics. To find molecular markers for Fritillaria species and elucidate the genomic characteristics of F. thunbergii,we sequenced the complete chloroplast (cp) genome of F. thunbergii and performed a comparative analysis with relatedplant taxa. The complete cp genome of F. thunbergii is 152,155 bp with a quadripartite structure, which is similar to otherplants, and was highly similar to previously reported Fritillaria cp genomes in regard to gene content, order, orientation,and GC contents. Comparative analysis of the cp genomes identified two genes, matK and rps16, that contained sufficientmarkers to distinguish between two herbal medicines (Fritillariae Thunbergii and Fritillariae Hupehensis Bulbus) and fiveFritillaria species. These results provide useful information for identification of Fritillaria species and quality control ofFritillariae Bulbus.