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동종 골수 이식한 백혈병에서 단반복 유전자로 살펴본 혼합 키메라 현상의 의의
장대영,이정신,서철원,이규형,이제환,지현숙,박찬정,한면수,최동원,김정균,최성준,김성배,김상위,김우건,김상희 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1
연구배경 : 동종 골수 이식한 백혈병에서 키메라 현상의 분석은 착상, 질병 재발과 이식 거부를 이해하는데 도움을 줄 수 있다. 최근 STR을 PCR로 증폭한 검사법에 의해 매우 민감하고 유용하게 혼합 키메라 현상을 검출할 수 있다고 보고되고 있으나, 이 방법의 민감도와 임상적 의의는 아직 완전히 정립되지는 않았다. 따라서 PCR-STR검사법이 혼합 키메라 현상을 민감하게 검출하는지 보고, 동종 골수 이식 후 혼합 키메라 현상의 변화 양상을 관찰하고, 키메라 현상의 임상적 의의를 보고자 하였다. 방법 : 형제로부터 동종 골수 이식한 급성 백혈병 6명과 만성 백혈병 2명의 모두 8명을 대상으로 하였다. 44개 골수 검체에서 phenol/chloroform방법에 의하여 단핵세포 DNA를 추출하였다. DNA는 CTT 삼중체, vWA와 amelogenin시발체를 이용하여 증폭하였다. 증폭 산물은 5% 폴리아크랄아미드 젤에서 전기영동 뒤 은 염색으로 확인하였다. 2명의 DNA를 여러 비율로 혼합하여 상기 검사를 하여 검출 가능 최소 농도를 결정하였다. 결과 : 상기 검사법의 검출 가능 최소농도는 0.25%였다. 혼합 키메라 현상은 8명중 4명에서 관찰되었다. 혼합 키메라 현상은 보인 1명에서 급성 및 만성 이식편대 숙주 질환이 나타났고, 완전 공여자 키메라 현상을 보인 1명에서 만성 이식편 대 숙주 질환이 나타났다. 점진적으로 증가하는 혼합 키메라 현상을 보인 2명은 이식 후 12개월에 질병 재발을 보였으나, 완전 공여자 키메라 현상을 보인 경우엔 재발이 관찰되지 않았다. 결론 : PCR-STR검사법은 동종 골수 이식 후 남아 있는 환자 세포를 검출하고 키메라 현상을 관찰하는데 유용한 방법이다. 또한 이 검사법은 동종 골수 이식 후 재발의 조기 진단, 이식편 대 숙주 질환의 평가와 면역 관용에 임상적으로 응용할 수 있으리라 생각한다. Background : Chimerism analysis after allogeneic bone marrow transplantation (BMT) for leukemia could be helpful understanding of the early marrow engraftment, disease relapse, and graft rejection. Recently, a PCR technique which amplifies short tandem repeats(STR) has been reported to be highly sensitive and reliable in detecting mixed chimerism. But its sensitivity and clinical significance has not been established. The purpose of this study was firstly, to confirm whether a PCR-STR in highly sensitive enough ti assess mixed chimerism, secondly to monitor the changing patterns of mixed chimerism after allogeneic BMT, and lastly to determine correlation between the chimeric status and the clinical outcome. Methods : A study was made eight patients (six with acute leukemia and two with chronic leukemia) who underwent unmanipulated allogeneic BMT along with matched donors. DNA was extracted from mononuclear cells of 44 bone marrow samples using a phenol/chloroform method. Amplification of DNA was done using CSFIPO-TPOX-TH01(CTT) triplex with or without vWA or amelogenin primer. The amplified product was separated on 5% polyacrylamide gel and was confirmed by silver staining. The sensitivity of CTT triplex method was determined by mixing DNA from two person in serial proportion using the same method. Results : The sensitivity for CTT triplex method was 0.25% Mixed chimerism was documented in 4 of 8 patients. ONe of four patients with mixed chimerism developed acute and chronic graft versus host disease (GVHD) and one of four patients with complete donor chimerism also developed chronic GVHD. Two patient with progressive mixed chimerism relapsed at 12 months post-BMT, whereas no patient with complete donor chimerism has relapsed. Conclusion : The study has found that PCR-STR was an effective method for the detecting residual host cell and monitoring the chimeric status after allogenic BMT. PCR-STR can also be clinically applicable in early prediction of relapse, appropriate assessment of GVHD, as well as tolerance after allogeneic BMT.
최성일,김중술,신민섭,조맹제 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.3
연구목적 : 우울 및 신체화와 분노 표현 방식 간에 유의한 연관성이 있음이 이전 연구들에 의해 보고된 바 있으며, 분노 억압 이외에 분노 표현이 신체병리의 발생과 관련이 있다는 상반된 연구결과도 제시되어 왔다. 그러나 정신과 환자들은 대상으로 두 요인을 모두 고려하여 이뤄진 연구는 거의 없는 실정이므로, 본 연구에서는 정신과 환자들을 대상으로 상태-특성 분노 및 분노표현방식과 우울 및 신체화 간의 관련성을 알아보고자 하였다. 방 법 : 정신과 환자 53명과 정상인 59명을 대상으로 정신진단 검사(SCL-90-R)의 우울과 신체화 증상을 측정하는 문항 25문항과 한국판 상태-특성 분노 표현 척도를 실시하여 두집단의 차이를 비교하였다. 분노와 우울 및 신체와 간의 관계를 알아보기 위해 각 집단별로 상관 및 회귀 분석을 실시하였다. 결 과 : 본 연구결과는 분노 억제가 우울 및 신체화 증상의 발생에 중요한 요인으로 작용하고 있음을 보이고있으나, 신체화에 비해 우울이 더 높은 관련성을 보이는 바, 이전 연구결과들의 해석에 주의를 요함을 시사한다. 분노 억제와 우울 및 신체화 간의 관계가 심리치료에 중요한 함의를 지닐수 있다. Objectives : In previous studies, significant correlations between depression or somatization and the mode of anger expression were reported. However few studies were done in psychiatric patients, while some evidences were found that anger expression as will as anger suppression were related to development of somatization. This study aimed to investigate the relationship of the state-trait anger and the mode of anger expression to depression and somatization in psychiatiric patients. Methods : The depression and somatization questionnaire of SCL-90-R and Korean Adaptation of the State-Trait Anger expression Inventory were administered to 53 psychiatric patients and 59 normals. The relationship of anger to depression and somatization was analyzed by correlation and regression analyses. Results : In comparison with the normal control group, the psychiatric patient group showed significant differences on trait anger temperament but no significant differences on other anger variables. In regression analysis, the anger-in score was related to depression and somatization. However, the degree of explanatory power was higher in depression than in somatization. In the case of analyzing the state-trait anger, anger-in was significant predictor variable for depression in psychiatric patient group. Conclusion ; These results suggested that anger-in is the important factor in the onset of depression and somatization. Rather than the relationship of anger-in and somatization, the relationship of anger-in and depression is higher ; therefore the caution is necessary in the interpretation of previous studies. The relationship of anger-in to depression and somatization may have important implications for psychotherapy.
Bochner 방법을 응용한 Shigella의 약제내성 제거
최정현,이제철,설성용 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1
To study the optimal curing condition on selective medium described by Bochner et al. and modified medium, six strains of S. flexneri and 2 strains of S. sonnei were tested for minimum inhibitory concentration(MIC) of quinaldic acid and cured resistance pattern. Also, changing patterns of elimination according to incubation temperature were investigated at 37℃ and 42℃. MICs of Shigella on original Bochner medium and modified medium were 50 5o 400㎍/㎖. But MICs of Shigella were above of 400㎍/㎖ on the Bochner's medium excluding tetracycline and sodium phosphate. There were no differences in MIC between at 37℃ and 42℃. On the Bochner's medium, losses of drug resistance were more effective than other medium and cured resistance pattern was partial or total of original resistance, regardless of incubation temperature. Frequencies of curing were low on modified Bochner's medium, but most of cured resistance pattern were total resistance of Shigella. Frequencies of curing at 42℃ were similar to 37℃, but some strains showed resistance loss only at 42℃. MICs of 8 strains of Shigella were above of 400㎍/㎖ and no strains detected resistance loss on the both tryptic soy agar and tryptic soy broth.
우리나라 人文係高等學校와 實業係高等學校 學生들의 健康生活習慣에 관한 比較硏究
崔聖濟 관동대학교 1990 關大論文集 Vol.18 No.1
The main purpose of this study was to invertigate the attitude of health core which academic, industrial high school students. For the purpose of this study, the randomly selected subject (N=846), were divided into two Sub-groups such as academic high school students (N=423), and industrial high school students (N=423). The results were as follow: 1. Method of attending school for the youths were bus (48.9%), walking (35.9%), about 30 minutes of attending school was 83%. 2. The average day study of the youths were two hours∼four hours (50%), academic high school students showed highet than industrial high school in study time. 3. With regard to meals, ane's meals regularly(44.2%), irregularly(55.8%), 50.7% was satisfaetory of meals. 4. With regard to eating between meals, doing(60.2%), after at ten o'clock in the evening(37.8%), evening and before at ten o'clock in the evening(36.8%) in eating time, take eating between meals was milk, bread, imtant noodle. 5. It's like among of meat, fish, fruit was pork, squid, a hir-tail, appl, berry. 6. With regard to drinking, drinking(69.5%) in academic high school students, (79.4%) in industrial high school students, in the case of the first drinking, middle school, the first high school was 54% in academic high school students, 65% in industrial high school students, capacity for drinking in So Ju was 32.3% low half bottle, 22.2% about a bottle, 13.2% about a half bottle, motive for drinking was Curiosity(26.1%), afflicition(17.7%), friend advice(14.5%) 7. With regard to smoking, smoking(37.8%) in academic high school students, (50.1%) in industrial high school students, in the case of the smoking, Curiosity(22.6%), affliction(8.9%), Caparity for smoking in academic high school students(9.7%), in industrial high school students(14.4%) in fifteen cigarettes over. 8. With regard to the other sex, doing(39.2%) in academic high school students, (50.1%) in industrial high school students, 88.5% of the Youeh was reduired necessity, channel of sex education was school(39.6%), magazine(17.4%), friend(17.1%). 9. With regard to counsel, with parents doing(58.5%), 34%, 15.7% of each satisfactory. 10. With regard to exercise regularly, doing(22.9%) in academic high school students, (32.2%) in industrial high school students, importance of physical activity (82%), acquired factors through physical activity were advance of physical fitness(54.8%). 11. Satisfaatory of leisure(25.2%) like TV program was show(26.4%), Cinema(23.8%), take pleasure in reading was literarary or norel(27.5%), magazine or sports book(23.5%), comic book(16.4%) 12. Make use of kisure facilities in during Vacation were theater(16.0%), school ground(13.8%), Camping(12.2%), satisfaetory of leisure(12.8%), holpful to experience(31.2%) in seuse of value for leisure, leisure activities of hope were theatrical performance or go to the movies(23.2%), travelling(21.0%), sports(10.9%).
방사무늬김 색소변이형 사상체의 생체 가시부 흡수스펙트럼
최성제,신종암,윤장택 여수대학교 1998 論文集 Vol.12 No.2
Comparative study was made on absorption spectra of the sporothallus of the wild-type (U-511) plant, and the red-type (H-25), the green-type (C-O giant), the yellow-type (ZY-1), the violet-type (ZV-1), the gray-type (ZGr-1), the light red-type (ZLR-1), the light green-type (C-31) and the light yellow-type (ZLY-1) mutants in P. yezoensis. The absorption was recorded with a double monochrometer recording spectrophotometer (Hitachi U-3210) not equipped with an integrating sphere attachment or a head-on photomultiplier. The red-, the green-, the yellow-, the gray- and the light yellow-type mutants showed the spectra having two absorption maxima in 530-583 nm region. The red- and the green-type mutants had the spectra with markedly low absorption in a 380-750 nm region. The violet- and the gray-type mutants were characterized by higher PE/Chl. α and PC/Chl. αratios. The light yellow-type mutant was marked by lower PE/Chl. α and PE/PC ratios, while the gray- and the violet-type by higher PE/Chl. α and the gray-type by PE/PC ratios. It is inferred : that the light yellow-type mutants might result from qualitative and quantitative variations of phycoerythrin : that the light yellow-type mutants might originate from qualitative variation of phycoerythrin and quantitative variation of chlorophyll α: that the violet-, the light green- and light yellow-type mutant might arise from quantitative variation of chlorophyll α.
코크스 폐수를 이용한 활성슬러지 모델 파라미터의 민감도 분석
오재일,이병준,심효영,최상교 대한상하수도학회 2002 상하수도학회지 Vol.16 No.3
Activated sludge model (ASM, IAWQ Task Group) was developed as a general mathematical model to express major biological processes in municipal wastewater treatment. Therefore this model could be applied for the design of a wastewater treatment plant and used for finding optimal conditions in plant operation. Since biological processes adopting biodegradation have been also used to treat lots of industrial wastewater such as coke's wastewater, it is worthy to examine the applicability of ASM on the industrial wastewater treatment. In order to evaluate this possibility, the sensitivity analysis of seven parameters (Y_H,μ_H, b_H, k_h, K_S, K_(O,H), K_x) in ASM was conducted with regard to the SCOD (soluble chemical oxygen demand) removal in coke's wastewater treatment. It has shown that μ_H (maximum specific growth rate) of ASM was the most sensitive one in the SCOD removal process, and relative sensitivity was decided in order of μ_H >> b_H, K_S > Y_H, K_(O,H), K_h, K_x, consequently. Based on the sensitivity analysis and calibration procedure of ASM with the data from coke's plant, the decided parameter values were valid for the proper simulation of this industrial wastewater plant, showing adequate fitting between real and simulated data. In summary, the mathematical model of ASM was suitable for the simulation of coke's wastewater treatment plant.
악성 혈액질환에서 성공적인 동종골수이식 후 숙주 기질 미세환경의 구축
조상희,이제중,남찬은,최경상,정익주,이일권,김진희,박종태,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1
인체의 골수는 간엽모세포를 함유하고 있으며 이들은 골수미세환경의 주된 세포들로 분화가 가능하여 조혈기능을 지지한다. 본 연구에서는 성별이 다른 동종조혈모세포이식 환경에서 조혈모세포의 완전 생착을 보이고, 이식 후 1년에서 8년이 지난 11예의 재생불량성 빈혈 및 백혈병 환자들을 대상으로 하여 골수에서 MSC를 분리하고 체외 확장을 통해 배양된 MSC에서 X 염색체 탐식자를 이용한 FISH 및 microsatellite polymorphism PCR 기법으로 그 기원을 확인하였다. 그 결과 조혈모세포는 완전히 공여자 기원으로 대치되었음에도 불구하고 MSC는 모두 수여자 기원임을 알 수 있어, 동종조혈모세포이식에서 미세환경의 구축은 수여자의 자가 생산에 의한 골수 간질세포에 의한 것으로 생각된다. Background: Human bone marrow (BM) contains mesenchymal stem cells (MSC) that can differentiate into various cells of mesenchymal origin. It remains a matter of controversy whether donor-derived stromal cells are capable of engraftment following hematopoietic stem cell transplantation (HSCT) or not. Methods: To determine if donor-derived stromal cells are transferred to the recipients of allogeneic HSCT, we investigated the characterization of MSC in 11 patients 1 to 8 years after sex mis-matched allogeneic HSCT in severe aplastic anemia and leukemia. Results: All patients had complete engraftment with donor- derived stem cells as shown by detection of donor type DNA in peripheral blood mononuclear cells. Following culture, MSC showed the expression of SH2 and SH4, but none of the hematopoietic markers of CD14, CD34, or CD45. MSC which can be differentiated to osteogenic lineage showed the genotype of recipient completely using FISH or PCR analysis. Conclusion: This study confirmed that MSC isolated from recipients of allogeneic HSCT in severe aplastic anemia and leukemia are not of donor genotype despite of full hematopoietic engraftment with donor type. Donor cells did not contribute to reconstitute the marrow microenvironment.
김제열,조상호,김건일,허경림,김현숙,조구영,최영진,이원용,임종윤 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2
Cardiac tumors, especially the primary tumors involving any part of the heart are extremely rare and its relative incidence has been reported to be approximately 0.02%. We report a patient with huge cardiac hemangioma who complained of shortness of breath, general weakness, and dizziness, Imaging study by echocardiography and computed tomography of thorax revealed a huge lobulating mass like a bunch of grapes in the right chamber of heart. The surgical exploration of thorax was performed and a histological diagnosis of spindle cell hemangioma was obtained by microscopy. The patient was treated by surgical resection of the tumor and doing well after surgery. Our experience indicated that prompt diagnosis and treatment of cardiac hemangioma is imperative for patients' prognosis.
운동전략이 기립자세의 기능적 전방 팔뻗기에 미치는 영향
박제상,권오윤,최흥식,김택훈 한국전문물리치료학회 2000 한국전문물리치료학회지 Vol.7 No.1
The purpose of this study is to determine whether movement strategies affect functional forward reach distance in a standing position. Forty-seven healthy subjects were selected for this study: 23 men and 24 women, with an average age of 22.3. Functional forward reach distances were measured as hip strategy and squat strategy(included knee and ankle movement strategy) in a standing position, respectively. The mean values of functional forward reach distance in hip strategy, squat strategy were 33.57㎝, 29.48㎝, respectively. There was significantly difference in functional forward reach distance between hip strategy and squat strategy(p<.001). There was no difference of functional forward reach distance between male and female in hip strategy, but there was significant difference in other strategy(p<.05). These results suggest that movement strategies should be considered during functional forward reach test in standing. Further study is required to determine whether movement strategies affect functional reach distance in elderly and disabled groups.
천연 Zeolite를 이용한 매립지 침출수의 고농도 암모니아성 질소 제거에 관한 연구
오재일,홍성효,이선구,최영화,안봉규 중앙대학교 기술과학연구소 2002 기술과학연구소 논문집 Vol.32 No.-
천연 제올라이트의 일종인 Clinoptilolite를 이용하여 매립지 침출수내의 암모니아성 질소를 제거하는 실험을 수행하였다. 고정상 칼럼을 이용한 인공 침출수와 실제 침출수 조건의 실험에서 배출농도가 유입농도의 5%인 시점을 파과점으로 상정할 때, 인공 침출수 조건에서의 파괴시간은 53hr, 실제 침출수 조건에서의 파과시간은 32.5hr으로 나타났다. 또한, 허용 파과점 및 소모점에서의 제올라이트 100g(건조중량)당 제거된 암모늄 이온 성분량은 인공 침출수의 경우 각각 79.30meq/100g, 93.34meq/100g이었으며, 실제 침출수의 경우 각각 48.60meq/100g, 57.60meq/100g으로 나타났다. 침출수 조건의 경우 인공 침출수에 비해 약 39% 가량의 제거 효율 저하를 보이고 있으며, 이는 실제 침출수에 존재하고 있는 공존 양이온의 영향으로 사료된다. 실제 침출수 내에서 공존 양이온의 용출 특성을 보면, Na, Ca, Mg의 용출량은 증가하나 K는 용출량이 감소하는 것으로 나타났다. This experiment was conducted to remove the ammonia nitrogen in the landfill leachate by Clinoptilolite which is a sort of the natural zeolite. In fixed-bed column test driven in the artificial and actual condition of the leachate, when the ejection concentration was supposed the point of 5% of the initial concentration as the breakthrough point, the breakthrough time was 53hr in the artificial condition of the leachate and was 32.5hr in the actual condition of the leachate. The ingredient volume of the removed ammonium ion per 100g(based upon the dry weight) of the zeolite in the allowed breakthrough point and exhaustion point were individually 79.30meq/100g, 93.34meq/100g in the artificial leachate and 48.60meq/100g, 57.60meq/100g in the actual leachate. In case of the actual condition of the leachate, the removal efficiency was decreased by 39% than the artificial condition of the leachate, and it seems to be caused by the coexist cation in the actual leachate. The ejection characteristics of the coexist cation in the actual leachate was observed that Na, Ca, Mg was increased and K was decreased.