Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

Magnetron sputtering법으로 제조된 Al-1%Cu/Tungsten Nitride 다층 박막

이기선,서수정,김장현,김남철 공주대학교 자원재활용신소재지역협력센터 2000 2차년도 센터 사업 성과집 Vol.2000 No.1

표면 탄성파 디바이스의 전극재료로 사용되는 Al-1%Cu(4000Å)/tungsten nitride 박막을 magnetron sputtering법으로 제조하고 전기저항을 평가한 결과 비정질상의 tungsten nitride 박막을 제조할 수 있었고, 비정질 형성을 위해서 질소비 (R = N2/(Ar+N2))가 10∼40% 정도 필요하였다. Tungsten nitride 박막의 잔류응력은 비정질이 형성되면서 급격히 감소되었다. 이러한 비정질 박막위에 Al-1%Cu합금막이 형성되었다. 다층막은 453K에서 4시간 동안 열처리함으로써 3.6μΩ-cm의 저항를 나타냈는데, 이는 박막내 결정립 성장과 결정 결함의 감소에 기인하였다. As a power durable-electrode in SAW filter. Al-1%Cu/tungsten nitride multi-layer thin film was fabricated by magnetron sputtering process. Tungsten nitride films had the amorphours phase at the nitrogen ratio, R, ranging from 10~40%. The amorphization could be controlled by nitrogen ratio. R = N2/(N2+Ar) as a sputtering process parameter. Residual stress in tungsten nitride abruptly decreased with the formation of amorphous phase. Al-1%Cu thin film was deposited on the amorphous tungsten nitride. After the multi-layed thin film was annealed for 4 hours at 453K, the resistivity decreased as 3.6 μΩ-cm, which was due to grain growth and reduced crystal defects.

糞便檢査에 있어서 세로판 厚層塗抹法과 簡易食鹽水浮游法의 比較檢討

李駿商,張基浩,朱炅煥,金洙鎭,林漢鍾 고려대학교 의과대학 1979 고려대 의대 잡지 Vol.16 No.1

Experiments had been made to evaluate more efficient method in stool examination for various helminth ova. Authors examined 2,016 nationwidely collected stool specimens by the two methods, cellophane thick smear method and brine floatation method. The results were as foliows; 1. Of 2,019 specimens examined by cellophane thick smear method, the positive rates of following helminth ova were; Ascaris lumbricoides (fertilized) 27.3% Ascaris lumbricoides (unfertilized) 13.6%, Trichuris trichiura 39.0%, Hookworm 0.1%, Enterobius vermicularis 0.05% Hymenolepis nana 0.04%, Taenla spp. 1.3% Clonorchis sinensis 1.1% and Metagonimus yokogawai 5.7%. And, A. lumbricoides (fertilized) 27.0%, A. lumbricoides (unfertilized) 3.8%, T. trichiura 38.1%, Hookworm 2.0%, Trichostrongylus orlentalis 0.6%, E. vermicularis 0.2%, H. nana 0.8%, and M. yokogawai 0.05% by brine floatation method. 2. The causes of inferior positive rates in some helminth ova by cellophane thick smear method were shortly discussed and suggest some opinions in improvement of detection ability. 3. The positive rates of following helminth ova were superior in cellophane thick smear method to those of brine floatation method: A. lumbricoides(fertilized), Taenia spp., C. sinensis and M. yokogawai. And, the positive rates of following helminth ova were superior in brine floatation method: Hookworm T. orientalis, H. nana and E. vermicularis. 4. Cellophane thick smear method has been known to be one of the most effective methods in mass stool examination. But, this method is not so effective in detection of certain species of helminth ova. Therefore, employment of brine floatation method added to cellophane thick smear method is desirable for the better result of mass stool examination.

β-CuZn 합금의 항복강도 이상거동에 미치는 변형률 속도와 온도의 영향

이기안,장영원,이종수 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.3

A series of tensile and load relaxation behaviors in B2 type β-CuZn alloy have been examined to clarify the strain rate & the temperature dependence of yield stress anomaly. It has been found in tensile tests that the region showing anomalous peak of yield stress moves to a higher stress and higher temperature region with increasing strain rate. The anomalous yielding region below peak temperature(T_p) revealed slightly positive strain rate dependence, while the region above T_p showed considerably positive strain rate dependence. The slip lines formed below peak temperatures (T_P) were clearly visible with naked eye(coarse slip lines), and became finer near T_p(fine slip lines), while they disappeared completely above T_p. Based on the different tendency of temperature and strain rate dependence of anomalous yielding as the above-mentioned results, two types of deformation modes were suggested and incorporated into the flow curve analysis, which was in accord well with the experimental results. The slightly positive strain rate dependence of yield stress anomaly indicated that the yield stress anomaly in β-CuZn alloy mainly seemed to be attributed to the vacancy hardening.

고농후사료에 대한 목탄 및 활성탄의 첨가 수준이 인공위내 소화율, 휘발성 지방산 및 개스 생산량에 미치는 영향

이수기,차상우,김선균 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 시험은 목탄 및 활성탄의 첨가가 사료의 반추위내 pH·발효성상·건물 및 영양소 소실율·가스 생산량에 미치는 영향을 조사하기 위하여 in vitro 조건에서 실시되었다. 활성탄과 목탄의 첨가수준은 0.50% 및 1.0%의 수준이었으며, 사료의 조농비율은 오차드 건초와 농후사료 비율은 2:8로 하였다. 그리고 처리간 유의성은 5% 수준에서 검정하였다. 시험 결과를 요약하면, 위액의 pH는 활성탄의 첨가로 유의하게 높아졌다. 그리고 암모니아태 질소는 활성탄 첨가에 의하여 유의하게 높아졌으며, 목탄첨가구는 모첨가구와 비교할 때 유의한 차이가 인정되지 않았다. 또한 VFA molar ratio에서는 전구간에 유의한 차이가 인정되지 않았으며, C2/C3 비율은 유의한 차이는 아니지만 활성탄구가 다소 낮아지는 경향을 나타내었다. 그러나 목탄첨가구에서는 대조구와 유의한 차이가 인정되지 않았다. 건물·조단백질·조지방·NDF·ADF 및 hemicellulose의 소실율은 활성탄 첨가에 의하여 유의하게 증가되는 경향을 나타냈다. 목탄첨가구에 있어서는 0.5%구보다는 1.0%구가 유의하게 높은 성적을 나타내었다. 그러나 hemicellulose의 소실율은 전구간에 유의한 차이가 없었다. 가스 생산량에 미치는 영향은 활성탄 및 목탄 첨가구가 대조구에 비하여 유의하게 많은 양을 나타내었으며, 활성탄 첨가구와 목탄 첨가구를 비교하면 유의한 차이는 아니지만 활성탄 첨가구가 높은 경향을 나타내었다. 위 결과에서 보듯이 활성탄은 가축의 생산성 향상에 유리한 조건을 제공하는 경향이 있지만, 이것을 좀 더 명확히 밝히기 위해서는 활성탄의 흡착능력·위내의 세균층에 대한 연구 및 많은 생체실험도 병행되어야 할 것으로 생각된다. This study was conducted to investigate the effects of the addition of activated charcoal (AC) and oak charcoal on in vitro ruminal fermentation characteristics, nutrient disappearance, and ruminal gas production. AC and oak charcoal were added at the levels of 0.50, and 1.00 % to experimental diet (roughage/concentrate ratio ; 2/8). Ruminal pH and ammonia-N tended to increase by adding AC(P<0.05). But oak charcoal did not affect the ruminal pH and ammonia-N. Although not significant, ruminal total VFA and molar percentage of butyric acid tended to decrease in AC diets, but molar percentage of acetate and propionate were not affected by adding AC. Ruminal degradation of dry matter, crude protein NDF, and ADF in AC diets tended to increase than in non-AC diet, however, no tendency in ruminal degradation of hemicellulose was observed. Ruminal gas production tended to increase in the AC and oak charcoal diets(P<0.05). Although there appeared some beneficial effects in adding AC to ruminant diets in this study, more works should be done with AC before we can make clear conclusion on the use of AC in the ruminant diets.

혈전 발생의 위험 인자가 없는 환자에서 급성 신우신염에 의해 유발된 신정맥 혈전증 및 폐색전증

이광수,김아름,이승연,정주홍,김미정,이진수,정문현,홍기천,조순구 대한감염학회 2009 감염과 화학요법 Vol.41 No.5

Renal vein thrombosis (RVT) is not an uncommon condition amongst patients with nephrotic syndrome or malignancy. Septic pulmonary embolism (SPE) is associated with risk factors such as intravenous drug use, pelvic thrombophlebitis, and suppurative processes in the head and neck. However, acute pyelonephritis is a rare cause of RVT and SPE. Case reports on RVT and SPE due to acute pyelonephritis are rare. In most of the earlier cases, patients had underlying conditions such as diabetes mellitus, renal carcinoma, calyceal stones, and hyperhomocysteinemia. We report a case of acute pyelonephritis complicated by RVT and SPE that occurred in a patient without any predisposing risk factors for thromboembolism. RVT and SPE were diagnosed using computed tomography and ventilation/perfusion scan. The patient recovered with antibiotics and anticoagulation therapy without any surgical interventions.

Vitamin-C가 Vitamin-B_2 代謝에 미치는 영향

李暎求,李貞洙,李寄容 梨花女子大學校 藥學硏究所 1963 梨花藥學硏究 Vol.- No.4

당밀의 첨가가 갈근탕박 사일리지의 품질과 산양의 기호성에 미치는 영향

김성복,문계봉,이봉덕,오홍록,배형철,이수기 충남대학교 농업과학연구소 2006 농업과학연구 Vol.33 No.1

Two experiments were conducted to investigate the effects of molasses supplementation to silage materials in ensiling Galgeuntang (herbal medicine) meal on the quality of silage, and its palatability in Korean native goats. In experiment Ⅰ, molasses was added at the levels of 0, 0.5, and 1.0% to Galgeuntang meal with three replicates per treatment. In experiment Ⅱ, three 1.5-yr-old Korean native goats were used to measure palatability. In experiment Ⅰ, lactic acid contents in molasses treatments were significantly(p<0.05) higher, and pH and butyric acid contents were lower than those of non-molasses treatment. In addition, molasses treatment increased total microbial cell counts in MRS medium for lactobacillus, but decreased total microbial cell counts in PDA medium for fungi. Molasses supplementation to silage materials increased in vitro dry matter disappearance. Molasses supplementation tended to increase silage intake in Korean native goat (experiment Ⅱ), but the difference was not significant(p>0.05). It is concluded that molasses supplementation to silage materials in ensiling herbal medicine meal could improve its preservability and palatability, the higher the better.

생쥐 대장 평활근 세포에서 내향 정류성 칼륨 전류의 특성 연구

이은주,김명,정명섭,조향훈,김기훈,하현철,김준수,최석,전제열 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Objectives: K^(+) channels play an important role in regulating cellular excitability. The aim of this study was to know whether or not inward rectifier K^(+) channel exists in colonic smooth muscle cells. Methods: Mouse colonic smooth muscle cells were isolated using collagenase, and then we recorded their membrane currents using a whole-cell patch clamp technique. Results: With 90 mM K^(+) in bath, hyperpolarization-induced inward currents from -120 mV to 20 mV with 400 ms duration at a holding potential of -10 mV showed rapid activation, inactivation and inward rectification. The inactivation showed single exponential time course. Reduction of external K^(+) to 60 mM decreased the amplitudes of the currents in whole test voltage range and shifted the reversal potential to more negative potential. The inactivation process and peak currents of hyperpolarization-induced inward currents were not affected by removing external Na^(+). External Ba^(+) blocked hyperpolarization-induced inward currents by dose-dependent manner and pure Ba^(+)-sensitive currents showed strong inward rectification. Cs^(+) also suppressed hyper- polarization-induced inward currents. Ba^(+) and Cs^(+)-induced inhibitiOn of hyperpolarization-induced inward currents was voltage-dependent, and the extent of inhibition increasing with membrane hyperpolarization. Conclusions: These results suggest that inward rectifier KU channels may exist in proximal colonic smooth muscle and may play an important role in regulating membrane potential.

北韓의 行政體系의 變化에 관한 硏究

李聖九,張基秀 弘益大學校 1987 弘大論叢 Vol.19 No.1

The major traits of the North Korean administrative organization can be listed as follows. To begin with, its power structure consists of dualistic system-the Party and the governmental organ. The organic interrelatedness between the Party and the governmental organ has always been strongly stressed. And the organic interrelatedness between them is considered, can most successfully be realized only though the merging of both heads of the party and the government into one. The control of administration by the Party is most complete. And in order to secure the control, the governing power of the administrative organs stems directly from the Party. The administrative system of North Korea has undergone reorganizations as many as eight times since its establishment. The reorganizations of the governmental organ, however, have all been carried out in order to suit and satisfy the aims solely of Kim Il-Sung. Another aspect to be noted is that the first revolutionary generation is lagely replaced by the younger generation comprising bureaucrats of either the Party or the government. As a result, the general trend is toward 'bureaucratization' or the progressive transformation of politics into administration. In terms of organizational change in the North Korea's governmental structure, the lack of efficiency, specialization, effectivity in its structures is noticeable. To make up for these weakness, the decentralization and autonomy of its structure is urgently called for-at least in the substructures beneath the State Cabinet. What obstructs the way, however, (the State Council) is the ever present necessity of strong control over the governmental bodies in order to secure its stability, for the long-drawn-out Kim Il-Sung regime has of necessity raised such harassing problems as the threatening signs of seditions and as the undermining counter-effects. This is the serious dilemma in which the North Korean government finds itself.