다당류를 이용한 생분해성 필름의 제조

신은경,김동운,김유은,나건,이기영 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-

In this study, we prepared biodegradable polysaccharide films with pullulan, κ-carrageenan, curdlan and chitosan and measured physical properties and antibacterial activity All of polysaccharide films showed the high biodegradability but antibacterial activity on bacteria was only observed at chitosan film. The permeability of the gas(O₂and C0₂) of polysaccharide films besides chitosan film showed 50∼100 times lower than polyethylene(PE) film. Therefore, we prepared two film types to give low gas permeability and antibacterial property at polysaccharide film : one type was the physically mixed film(k-carrageenan +chitosan) and other type was the chitosan coated κ-carrageenan film. Chitosan coated κ-carrageenan film showed low gas permeability and antibacterial activity.

남성화 암쥐의 모리스 수중 미로 수행력 : 성에 따른 공간기억력과 테스토르테론 효과의 차이

신성현,박제민,정우영,박문일,서유나,김성곤,강철중 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objectives : It is widely accepted that gonadal steroids are involved in organization and activation of sexual dimorphism in the brain areas related with spatial leaming. This study aimed to test the following hypothesis : 1) spatial memory is affected by sex, 2) this difference is related with brain differentiation by exposure to testosterone in the neonatal period, and/or 3) the sexual difference of spatial memory is the result of the interaction between organization of sexual dimorphism in the brain and the circulating testosterone after growing up. Methods : Testosterone enanthate 100 ㎍, ('androgenized') orvehicle ('control') was randomly administered to 28 female and 25 male rat pups within 24 hour of birth. The escape latencies (EL) in Morris water maze were measured in 15 and 16 week of age with vehicle ('baseline') and with 10 μmol/kg of testosterone ('with testosterone'), respectively. Each session Consis-ted of 10 trials, and means of the last 5 trials were analyzed. Results : The baseline EL of the males were significantly shorter than females regardless ofneonatal exposure to testosterone. With testosterone, EL of the androgenized females were significantly shorter than control females and comparable to those of males. Conclusion : Neonatal exposure to testosterone in female rats may develop a tesosterone-dependent male-like spatial leaming system.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

유해화학물질의 계통분석

박성우,박유신,이진훈,김남이,김유나,임미애,이상기,유재훈,진광호,신호상,노동석 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

Detection of Near-membrane Calcium Ions in Live Tissues with a Two-Photon Fluorescent Probe

Shin, Yu-Na,Lim, Chang-Su,Tian, Yu Shun,Rho, Won-Young,Cho, Bong-Rae Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.3

A two-photon fluorescent probe (ACaCL) that can detect near-membrane $Ca^{2+}$ is reported. This probe can be excited by 780 nm fs pulses, shows high photostability and negligible toxicity, and can visualize near-membrane $Ca^{2+}$ in live cells and deep inside live tissues by two-photon microscopy.

Detection of Near-membrane Calcium Ions in Live Tissues with a Two-Photon Fluorescent Probe

Yu Na Shin,임창수,Yu Shun Tian,Won Young Rho,조봉래 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.3

A two-photon fluorescent probe (ACaCL) that can detect near-membrane Ca2+ is reported. This probe can be excited by 780 nm fs pulses, shows high photostability and negligible toxicity, and can visualize near-membrane Ca2+ in live cells and deep inside live tissues by two-photon microscopy.

간호사가 인식하는 간호 관리자와 동료간 돌봄 행위가 조직 사회화와 간호 업무 성과에 미치는 영향

신나연(Shin, Na Yeon),유소영(Yu, Soyoung),강성숙(Kang, Seong Suk),이승신(Lee, Seung Shin),박민정(Park, Min Jeung),이대연(Lee, DaeYeon),남선미(Nam, Sun Mi) 한국간호행정학회 2020 간호행정학회지 Vol.26 No.2

Purpose: The study was examined to investigate the influence of nurse manager and peer group caring behaviors on organizational socialization and nursing performance Methods: The subjects were 286 clinical nurses from a general hospital in S city. The collected data were analyzed using descriptive statistics, t-test, ANOVA, Pearson correlation and hierarchical regression analysis with the SPSS 25.0 program. Nurse manager and peer group caring behaviors, organizational socialization, and nursing performance were measured using organizational climate for caring scale, peer group caring interaction scale, organizational socialization and performance measurement scale for hospital nurses. Results: There was no significant difference in organizational socialization between nurse manager and peer group caring behaviors. The significant predictors of nursing performance were position (β=-.26), nurse manager caring behaviors (β=.23), and peer group caring behaviors (β=.17). These variables explained 23% of the variance in nursing performance. Conclusion: The results suggest that it is necessary to increase both manager and peer group caring behaviors in order to improve nursing performance.

단순 손동작 반복이 말소리장애 아동과 일반 아동의 말소리산출의 정확성과 유창성에 미치는 영향

신유나(Shin Yu-Na),하지완(Ha Ji-Wan) 대한신경계작업치료학회 2019 재활치료과학 Vol.8 No.2

목적 : 본 연구에서는 말소리장애 아동과 일반 아동을 대상으로 어휘인출 시 단순 손동작(hand tapping)을 반복하게 하는 것이 조음정확도와 유창성에 어떠한 영향을 미치는지에 대해 알아보았다. 손동작을반복하면서 어휘를 산출하는 것은 주의를 분산시키는 이중과제에 해당하기 때문에, 주의력 저하가 보고된 말소리장애 아동의 경우 일반 아동과 다른 행동 양상을 보이는지를 파악하고자 하였다. 연구방법 : 4, 5, 6세의 말소리장애 아동 15명과 일반 아동 15명이 본 연구에 참여하였다. 조음복잡성이 높은 어휘와 낮은 어휘를 각각 15개씩, 총 30개를 선정하여, 이에 대한 대면이름대기 과제를 실시하였다. 첫 번째 실험조건에서는 단순 손동작을 반복하지 않고, 두 번째 실험조건에서는 손바닥으로 책상을 두드리면서 그림 이름을 말하도록 하였다. 대상자의 반응에 대해 자음정확도, 비유창성 정도, 정확성변화와 유창성 변화의 상관관계를 측정하여, 두 실험조건에 따른 두 집단 간 수행력을 비교하였다. 결과 : 첫째, 말소리장애 집단과 일반 집단 모두 손동작 반복 과제와 손동작 비반복 과제 간 자음정확도에는 유의한 차이가 없었다. 둘째, 일반 집단은 손동작 반복 과제에서 비유창성이 유의하게 증가하였으나, 말소리장애 집단은 차이가 없었다. 셋째 손동작 반복에 따른 자음정확도 변화와 비유창성 변화는 일반 집단의 경우 유의한 양의 상관관계가 있었으나 말소리장애 집단은 아무런 상관을 보이지 않았다. 결론 : 본 연구에서 주의력 분산을 위해 사용한 단순 손동작 반복은 결과적으로 집단에 따라 대상자의 목표행동에 방해가 될 수도, 혹은 그 반대로 도움이 될 수도 있었다. 어휘인출에 대한 손동작 반복의영향이 두 집단 간 다른 양상으로 나타난 만큼 이에 대한 심층적 논의가 필요할 것이다. the fluency of speech production in children with speech sound disorder(SSD) and their typically developing peers(TD). Methods : The study subjects were 15 SSD children and 15 TD children aged 4, 5, and 6 years of age. Subjects were asked to give a picture name without hand tapping in the first experimental condition, and with hand tapping in the second experiment condition. Results : The results showed that hand tapping significantly increased disfluency in TD, whereas in SSD. it did not affect the accuracy or fluency of speech production. In addition, TD demonstrated a significant positive correlation with the changes of accuracy and disfluency due to hand tapping, whereas SSD had no correlation. Conclusion : We discussed the possibility that hand tapping could serve as an obstacle distracting attention from SSD and TD, acting as a motor gesture to facilitate phonological processing when facing the difficulty in lexical retrieval for SSD.

주의분산 과제에 따른 말소리장애 아동과 일반 아동의 자음정확도 비교

신유나(Yu Na Shin),하지완(Ji Wan Ha) 한국언어치료학회 2022 한국언어치료학회 학술발표대회 논문집 Vol.2022 No.11

주의분산이 말소리장애 아동과 일반 아동의 조음정확도 및 유창성에 미치는 영향

신유나(Yu Na Shin),하지완(Ji Wan Ha) 한국언어치료학회 2016 한국언어치료학회 학술발표대회 논문집 Vol.2016 No.12