Immature Oocyte-specific Zap70 and Its Functional Analysis in Regulating Oocyte Maturation

김유나,김은주,김은영,이현서,김경화,이경아 한국발생생물학회 2009 발생과 생식 Vol.13 No.3

Previously, we obtained the list of genes differentially expressed between GV and MII oocytes. Out of the list, we focused on functional analysis of Zap70 in the present study, because it has been known to be expressed only in immune cells. This is the first report about the expression and its function of Zap70 in the oocytes. Synthetic 475bp Zap70 dsRNA was microinjected into the GV oocytes, and the oocytes were cultured in vitro. In addition to maturation rates, meiotic spindle and chromosome rearrangements, and changes in expression levels of transcripts of three kinases, Erk1/2, JNK, and p38, were determined. Zap70 is highly expressed in immature GV oocytes, and gradually decreased as oocyte matured. When dsRNA of Zap70 was injected into the GV oocytes, Zap70 mRNA specifically and completely decreased by 2 hr and its protein expression also decreased significantly. Absence of Zap70 resulted in maturation inhibition at meiosis I (57%) with abnormalities in meiotic spindle formation and chromosome rearrangement. Concurrently, mRNA expression of Erk2, JNK, and p38, were affected by Zap70 RNAi. Therefore, we concluded that Zap70 is involved in MI-MII transition by affecting expression of MAP kinases.

Microstructural Modification of NiAl Layered Double Hydroxide Electrodes by Adding Graphene Nanosheets and Their Capacitative Property

김유나,김석 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.2

NiAl layered double hydroxide (LDH) composite electrodes containing various contents of graphene nanosheets (GNS) were prepared by a hydrothermal method. The micro-structure and morphological properties were examined by FE-SEM, FE-TEM, XRD, and FTIR. Electrochemical analysis was also carried out by cyclic voltammetry, impedance, and cycle life measurement. The as-prepared composite that contained 500 mg of graphene (denoted as NiAl/G-50) achieved the highest specific capacitance of 1147 F/g among the various NiAl LDH/GNS composites. Besides, the NiAl LDH/GNS composite exhibited the lower diffusion resistance, improved rate capability, and good cyclic stability (83% of initial capacitance after 2000 cycles). Considering the morphological data and the improved capacitative properties together, we concluded the synthesized NiAl LDH/GNS composites would be a promising electrode material for supercapacitors.

Resveratrol suppresses breast cancer cell invasion by inactivating a RhoA/YAP signaling axis

김유나,최소라,조경화,조도연,강재구,박창교,이회영 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Hippo/YAP signaling is implicated in tumorigenesis and progression of various cancers. By inhibiting a plethora signaling cascades, resveratrol has strong anti-tumorigenic and anti-metastatic activity. In the present study, we demonstrate that resveratrol decreases the expression of YAP target genes. In addition, our data showed that resveratrol attenuates breast cancer cell invasion through the activation of Lats1 and consequent inactivation of YAP. Strikingly, we also demonstrate that resveratrol inactivates RhoA, leading to the activation of Lats1 and induction of YAP phosphorylation. Further, resveratrol in combination with other agents that inactivate RhoA or YAP showed more marked suppression of breast cancer cell invasion compared with single treatment. Collectively, these findings indicate the beneficial effects of resveratrol on breast cancer patients by suppressing the RhoA/Lats1/YAP signaling axis and subsequently inhibiting breast cancer cell invasion.

Comparison between weekly versus 3-weekly paclitaxel in combination with carboplatin as neoadjuvant chemotherapy in advanced ovarian cancer

김유나,이용재,이정윤,남은지,김상운,김성훈,김영태 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.2

Objective: To compare the efficacy and toxicity of dose-dense weekly paclitaxel and 3-weekly carboplatin (ddPC) as neoadjuvant chemotherapy (NAC) with the standard 3-weekly regimen. Methods: A retrospective study of patients diagnosed with stage IIIc and IV ovarian cancer who received at least one cycle of NAC followed by interval debulking surgery between August 2015 and January 2018 was conducted. Patient characteristics, clinical and pathological response to NAC, surgical and survival outcome, and adverse event were compared. Results: A total of 23 patients in the ddPC group and 50 patients in the standard group received a median of 3 cycles of NAC. Rate of grade ≥3 neutropenia was significantly higher in the ddPC group than the standard (82.6% vs. 22.0%, p<0.001). Patients in the ddPC group underwent dose-reduction more frequently (34.8% vs. 4.00%, p=0.001). Normalization of cancer antigen-125 post-NAC occurred more frequently in the ddPC group (73.9% vs. 46.0%, p=0.030). No residual disease rate (43.5% vs. 60.0%, p=0.188) and chemotherapy response score of 3 (34.8% vs. 26.0%, p=0.441) were not statistically different between two groups. There was no statistical difference in progression free survival (PFS) at 2 years (36.3% vs. 28.4%, p=0.454). Cox proportional hazard model showed that ddPC was not a significant determinant of PFS (p=0.816). Conclusion: There was no difference between both regimens in terms of NAC response and survival outcomes. However, ddPC group showed higher hematologic toxicity requiring dose reduction.

용역근로자의 보호에 관한 연구

김유나 한국사회법학회 2018 社會法硏究 Vol.0 No.36

Today, the externalization of employment has worsened the working conditions of subcontract worker employed by service company. They are not effectively guaranteed even the labor's three rights of article 33 of the Constitution. In the process of changing service company, disputes about employment succession among subcontract worker frequently occur. But in reality, there is a lack of legal mechanisms to resolve the employment succession dispute. In addition, it should be possible to conduct collective bargaining for the protection of employment, such as organizing labor unions, requiring direct employment with the prime contractor, or requiring new service providers to obtain a guarantee of employment status. The legislation for the expansion of employer concept will also be needed under the Labor Union Act to enable collective agreements containing these contents. 오늘날 고용의 외부화 현상으로 인하여 용역업체에 고용된 용역근로자들의 근로조건은 더욱 열악해지고 있으며, 이들이 조직한 노동조합의 경우에는 원청기업을 상대로 한 단체교섭이 허용되지 않아 노동3권도 효과적으로 보장받지 못하고 있다. 한편, 용역업체의 변경 과정에서 용역근로자의 고용승계에 관한 분쟁도 빈번하게 발생하나, 이를 해결하기 위한 법제도는 마련되어 있지 못한 상황이다. 이러한 문제들을 해결하기 위하여 종래 다양한 해석상 노력이 있었으나, 판례는 원청기업과 용역근로자 사이의 직접적인 근로관계의 인정에 매우 엄격할 뿐만 아니라 원청기업의 노동조합법상 사용자성 또한 지배개입의 부당노동행위에 한하여 좁게 인정할 뿐이다. 따라서 용역업체 변경 과정에서의 용역근로자 보호 및 용역근로자의 노동3권의 실질적인 보호를 위한 입법이 필요하다.

잠재프로파일분석을 활용한 세대집단별 가족가치관 유형화 연구

김유나,박애리 한국거버넌스학회 2022 한국거버넌스학회보 Vol.29 No.2

우리 사회는 오랜 시간에 걸쳐 전통적 가족에 대한 가치 변화가 일어나고 있는 현상을 인식하고 있고, 이러한 현상이 우리 사회에 미치는 영향에 대한 논의를 활발하게 이어가고 있다. 그러나 이러한 현상이 세대집단별로 어떠한 양상으로 일어나고 있는지 실증적으로 규명한 연구는 매우 부족한 상황이다. 이에 본 연구는 여성가족부의 「2020년 가족실태조사」 데이터를 활용하여 결혼관, 가족의례 및 문화, 성역할 인식 및 부모 부양과 자녀양육에 대한 인식을 중심으로 20대부터 70대 이상까지 세대별 가족가치가 유형화 되는지살펴보았다. Mplus를 이용하여 잠재프로파일분석(Latent Profile Analysis, LPA)을 실시한 결과, 20대와 30대가 다른 세대에 비해 비전통적인 성향이 강한 유형화를 보였다. 결혼관과 가족의례 및 문화에 있어개방적이고 자유로우며, 성평등적이고, 부모부양과 자녀관에 있어 의무와 당위성이 약화된 개인주의적 성향으로 나타났다. 다만, 성평등에 있어서는 20대가 30대보다 좀 더 강한 성평등적 속성을 보였다. 40대와50대에서는 가족가치관에 있어 전통적인 성향과 비전통적인 성향이 동시에 나타났으며, 50대가 전통적인성향이 좀 더 강했다. 결혼관과 가족의례 및 문화에 있어 40대 대다수는 자유주의적 성향으로 나타난 반면, 50대는 대다수가 전통적인 성향으로 나타났다. 부양관과 자녀관에 있어서도 비슷한 패턴을 보였다. 다만, 성역할에 있어서는 40대와 50대 모두에서 성평등적인 유형화가 우세하게 나타났다. 마지막으로, 60대와70대에서는 전반적으로 전통적인 유형화가 우세하게 나타났다. 특히, 결혼관과 가족의례 및 문화에서 전통적인 가치를 강하게 유지하는 것을 알 수 있었다. 부양관과 자녀관에 있어서는 여전히 대다수가 전통적인성향으로 유형화 되었지만, 소수가 개인주의적 성향으로 유형화 되었다. 그러나, 성역할에 있어서는 60대의 상당수가 성평등한 유형화에 속했고, 70대에서도 성평등한 유형화가 나타났다. 이와 같은 연구 결과를토대로 세대별 가족에 대한 가치관을 반영한 가족정책 개발에 관한 정책적 함의를 논하였다.

체육영재 프로그램 적용에 따른 다중지능과 뇌파활성도

김유나,정성현 한국초등체육학회 2017 한국초등체육학회지 Vol. No.

본 연구는 체육영재 프로그램 적용에 따른 체육영재 학생들의 다중지능과 뇌 활성도를 파악하여 향 후 운동선수 양성 프로그램, 우수선수발굴시스템 개발 및 보완에 기초자료를 제공하고자 하는 데 그 목적이 있다. 연구의 대상은2012년 A대학의 체육영재센터로 선정하여 총 30명을 유목적표집하였다. 이 중 제외한 자료의 경우 전문가 회의를거쳐 저학년의 경우 검사지 이해 부족과 뇌파검사의 집중력 부족으로 검사 결과에 부정적인 영향을 미칠 것으로 판단되어 저학년 2학년 11명을 제외하고 19명의 자료를 추출하여 사용하였다. 이러한 과정을 거쳐 얻은 연구의 결과는 다음과 같다. 첫째, 체육영재 프로그램에 따른 다중지능의 차이는 신체운동지능, 언어지능, 자아성찰지능, 대인관계지능이 유의한 차이가 있는 것으로 나타났다. 둘째, 체육영재 프로그램에 따른 뇌파활성도에서 알파파, 베타파가 유의한 차이가 있는 것으로 나타났다. 본 연구의 결과와 같이 다중지능의 향상과 알파파, 베타파의 활성은 체육영재프로그램을 통해 체육영재의 집중력, 심신의 안정 이 외 전문실기 경기에서 나타날 수 있는 불안, 걱정 등을 해소시킬 수 있는 능력이 향상된 것으로 판단된다. The purpose of this study was to analyze the multiple intelligences and brain activity based on Sports Talented program. Ultimately intended to provide a basis for the development of Sports Talented program. Students study the subjects were sampled a total of 30 people in 2012, A University of Sports Talented Center.11 of 30 people second grade data were excluded because of insufficient concentration. These findings obtained by the process is as follows. First, the multiple intelligence tests physical exercise intelligence, language, intelligence, self-reflection intelligence, interpersonal intelligence showed that no significant difference. Second, the brain wave activity test results showed that significant differences is the alpha wave and beta waves. As a result of this study, it seems that the improvement of multiple intelligence and the ability of alpha wave and beta wave activity to resolve the concentration, mental and physical stability, anxiety, and anxiety of the athletic gifted are improved.

CAR-T 세포유전자치료제 허가자료 사례연구

김유나,황채원,전보경,한다혜,진다영,이창재,최윤정,이상효,권경희 한국에프디시규제과학회(구 한국에프디시법제학회) 2022 FDC법제연구 Vol.17 No.1

Factors Associated with Cheyne-Stokes Respiration in Acute Ischemic Stroke

김유나,김성헌,류동열,이서영,임경빈 대한신경과학회 2018 Journal of Clinical Neurology Vol.14 No.4

Background and Purpose Cheyne-Stokes respiration (CSR) is frequently observed in patients with acute stroke. There have been conflicting opinions about the associations of CSR with the location and size of the lesion. We aimed to better define the clinical relevance and pathogenesis of CSR in acute stroke. Methods We investigated patients who had been admitted with acute ischemic stroke and received an overnight sleep apnea test. We collected data on demographics, risk factors, etiologic subtypes, initial vital signs, clinical course of the stroke, and parameters associated with respiratory events during the sleep apnea test. We performed a multivariate logistic regression analysis to determine the factors associated with CSR. Results Among 182 patients, 35 patients showed CSR in sleep apnea testing. Large-artery atherosclerosis or cardioembolism, bilateral hemispheric involvement, atrial fibrillation, low left-ventricle ejection fraction (LVEF), and left atrium (LA) enlargement were all associated with the presence of CSR. Multivariate analysis revealed that the previous modified Rankin Scale (mRS) score, bilateral hemispheric involvement, low LVEF, and LA enlargement were significantly associated with CSR. Subgroup analysis with large-artery atherosclerosis without cardiac disease revealed that the previous mRS score is the only independent factor associated with CSR. Conclusions CSR frequently occurs in strokes involving large arteries or due to cardioembolism, regardless of the location and severity of the stroke. Predisposing conditions such as preexisting neurologic disability, low LVEF, and LA enlargement are associated with CSR in acute stroke.

Relationship between Progressive Changes in Lamina Cribrosa Depth and Deterioration of Visual Field Loss in Glaucomatous Eyes

김유나,신중원,성경림 대한안과학회 2018 Korean Journal of Ophthalmology Vol.32 No.6

Purpose: To investigate the relationship between the progression of visual field (VF) loss and changes in lamina cribrosa depth (LCD) as determined by spectral-domain optical coherence tomography (SD-OCT) enhanced depth imaging in patients with primary open angle glaucoma (POAG). Methods: Data from 60 POAG patients (mean follow-up, 3.5 ± 0.7 years) were included in this retrospective study. The LCD was measured in the optic disc image using SD-OCT enhanced depth imaging scanning at each visit. Change in the LCD was considered to either ‘increase’ or ‘decrease’ when the differences between baseline and the latest two consecutive follow-up visits were greater than the corresponding reproducibility coefficient value (23.08 µm, as determined in a preliminary reproducibility study). All participants were divided into three groups: increased LCD (ILCD), decreased LCD (DLCD), and no LCD change (NLCD). The Early Manifest Glaucoma Trial criteria were used to define VF deterioration. Kaplan-Meier survival analysis and Cox’s proportional hazard models were performed to explore the relationship between VF progression and LCD change. Results: Of the 60 eyes examined, 35.0% (21 eyes), 28.3% (17 eyes), and 36.7% (22 eyes) were classified as the ILCD, DLCD, and NLCD groups, respectively. Kaplan-Meier survival analysis showed a greater cumulative probability of VF progression in the ILCD group than in the NLCD (p < 0.001) or DLCD groups (p = 0.018). Increased LCD was identified as the only risk factor for VF progression in the Cox proportional hazard models (hazard ratio, 1.008; 95% confidence interval, 1.000 to 1.015; p = 0.047). Conclusions: Increased LCD was associated with a greater possibility of VF progression. The quantitative measurement of LCD changes, determined by SD-OCT, is a potential biomarker for the prediction of VF deterioration in patients with POAG.