한국 주요정신장애의 유병률 및 관련요인 : 2006 전국정신질환역학조사

조맹제,장성만,함봉진,정인원,배안,이영문,안준호,원승희,손정우,홍진표,배재남,이동우,조성진,박종익,이준영,김진영,전홍진,이해우 大韓神經精神醫學會 2009 신경정신의학 Vol.48 No.3

Objectives The aims of this study are to estimate the prevalence of the DSM-IV psychiatric disorders in the Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI), and to compare those with previous studies. Methods The Korean Epidemiologic Catchment Area study Replication (KECA-R) was Conducted between August 2006 and April 2007. The sampling of the subjects was carried out across 12 catchment areas. A multistage, cluster sampling design was adopted. The target Population included all eligible residents aged 181o 64 years. Face-to-face interviews were conducted with the Korean version of Composite International Diagnostic Interview (K-CIDI) based on the DSM-IV (N=6,510, response rate=81.7%). Results A total of 6,510 participants completed the interview. The lifetime and 12-month prevalence rates for all types of DSM-IV disorders were 30.0% and 17.3%, respectively. Those of Specific disorders were as follows : 1) alcohol use disorder, 16.2% and 5.6% ; 2) nicotine use disorder, 9.0% and 6.0% ; 3) specific phobia, 3.8% and 3.4% ; 4) major depressive disorder, 5.6% and 2.5% ; and 5) generalized anxiety disorder, 1.6% and 0.8%. Data relating to nicotine and alcohol use disorder revealed a very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among females than males. Conclusion The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in the distribution of psychiatric disorders across the country and times were observed.

제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

3,4-Dihydroxyphenyl-L-alanine의 효소적 생산에 대한 반응첨가물의 영향

이승구,노현수,홍승표,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

재조합 대장균에서 과발현된 Citrobacter freundii KCTC 2006 유래의 tyrosine phenol-lyase(E.C.4.1.99.2) 이용 3,4-dihydroxyphenyl-L-alanine (L-DOPA)의 생산에 대한 반응첨가물의 영향을 조사하였다. 반응액 중 효소 및 조효소 농도의 영향을 조사한 결과, 효소농도는 약 2 units/ml이 적합하였으며, 조효소인 pyridoxal -5-phosphate 는 0.1 mM 이상이 필요하였다. Pyrocatechol과 결합하여 안정한 복합체를 형성하는 sodium borate는 pyrocatechol에 의한 효소의 불활성화를 감소시키는 효과가 있었으나, 효소의 반응성을 현저히 저하시켜 L-DOPA 생산의 관점에서는 불리한 단점도 있었다. 한편, 알콜류 등의 유기용매가 L-DOPA 합성반응에 미치는 영향을 조사한 결과 methanol을 5% 농도로 반응액에 첨가하였을 때, 효소의 반응과 안정성이 크게 증가하여 L-DOPA 합성반응이 지속적으로 수행됨으로써 고농도의 L-DOPA를 효율적으로 생산할 수 있게 되었다. 생산된 L-DOPA의 약 77%가 불용성 상태로 침전되어 쉽게 회수할 수 있었으며, 침전된 L-DOPA를 1N HCI에 용해한 후 재결정화 함으로써 최종적으로 99.96%의 고순도 L-DOPA를 생산할 수 있었다. The enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) was examined for the effects of the reaction additives such as sodium borate, alcohol, and organic solvents. The enzyme used was tyrosine phenol-lyase of Citrobacter freundii KCTC 2006 produced in Escherichia coli. The amounts of tyrosine phenol-lyase and pyridoxal -5-phosphate were optimized to 2.0 units/ml and 0.1 mM, respectively, for the synthetic reaction. Sodium borate, a substance that forms a complex with pyrocatechol, reduced the enzyme deactivation by pyrocatechol although it seriously inhibited the enzyme activity. Among the organic solvents tested, dimethylsulfoxide, dimethylformamide, and alcohol increased the productivity of the L-DOPA synthesis. In a reaction system with 5% methanol, L-DOPA concentration increased up to 210 mM after 24 hours, and 77.1% of which was separated as precipitates. The L-DOPA was purified to 99.96%

FA/MA 방법에 의한 부산시 부유분진의 오염원 할당

정장표, 이승훈, 윤항묵 경성대학교 환경문제연구소 2000 環境硏報 Vol.10 No.1

The object of this study was to identify source categories of Busan area through FA/MA methodology which contribute to the concentration of suspended particles at receptor. According to the purpose of this study, twenty-seven chemical species such as elements, anions, and total carbon of chirty-six PM10 and TSP data sets sampled at Bumchun receptor site for a 24hr period from May to Aug. 1992, were analyzed and applied to FA/MA model. From this study, the following conclusions were obtained. From the factor analysis for measured data, steel, mill-related, transportation, soil dust resuspension, marine, fuel oil, secondary aerosol source, and etc., of which total variance explained by the identified factors was calculated as 84 ∼ 91%, were identified. And the trend was shown that the more the number of input trace materials was, the more difficult the identifications of factors were, relatively. Selecting the representive tracers for identified factors based on the factor loadings, the following equations were obtained by use of stepwise method in multiple linear regression for the cases. TSP(㎍/㎥) = 27.996+0.033Mg +0.016Cl-+0.O05SO42 Case 1 TSP(㎍/㎥) = 11.779 + 2.013Ti + 0.034Na + 0.026Mg + 0.006ca Case 2 PM10(㎍/㎥) = 3.130+0.O04 SO42 +0.010 Cl- + 1.812Ti Case 1 PM10(㎍/㎥) = -14.028+1.515Ti +0.D38Na + 0.O09 Cl- + 0.003 SO42- Case 2 In comparison with the results of FA/MR model with the change of the newly added input tracers, the case with TSP showed relatively unstable results of factor analysis for the case studies because of insufficient number of data available to complete a factor analysis for the tracers used.

분뇨처리장의 공정개선에 의한 처리효율 향상에 관한 연구

이찬원,김승현,김창수,문성원,전홍표,윤종섭 한국환경과학회 2004 한국환경과학회지 Vol.13 No.6

There is a need to improve the efficiency of the existing sanitary treatment facilities, because the effluent standard becomes more stricter and septic sludge increased. Thus, operating processes of sanitary treatment system in M city changed with installation of additional facilities. Process modifications were as follows: Dilution water was added to the next process after primary aeration tank. Some secondary sedimentation sludge was recycled to primary aerator so that most of the organics were stabilized in primary aeration tank under automatic control of dissolved oxygen. The line of effluent from dewatering process flowing to the activated sludge tank was changed to the primary aerator. The primary sedimentation sludge line was linked to a thickener. Polymer was added to the activated sludge tank. The effluent of primary aerator and aerobic digester was recycled from the 5th to the 1st sector. As consequencies of above process modifications, the improvement of removal efficiency was achieved as BOD 54%, COD 42%, SS 61%, T-N 39%, and T-P 12%, respectively.

3차원 가상 치과모형 계측 값의 정확성 유효 검정

이주훈,장미숙,백기석,이승표 대한해부학회 2004 Anatomy & Cell Biology Vol.37 No.2

재래식 시장 지하 상인의 호흡기증상 및 폐기능 조사 연구

이승관,이창규,조경진,임현표,서장훈,최윤수 高麗大學校 倂設 保健大學 保健科學硏究所 1998 保健科學論集 Vol.24 No.1

The prevalence of respiratory symptoms and pulmonary function were studied in a group of 55 whole-sale market merchants Working in underground. As a control group, 55 whitecollar workers was also included in this study. Following results were obtained. A higher complaint for respiratory symptoms(cough, sputum, chest tightness, shortness of breath) was found among merchants than control workers. The measurement value for FVC, FEV1.0, FEV 1.0% in the merchants were reveal to lower figure in comparison with control groups. The measurement Value for MVV, PEF in the merchants were lower than control groups, but was not significant statistically. The pattern of pulmonary ventilation disturbance in the whole-sale market merchant were the same as followings : restrictive disturbance(6 person), abstrictive disturbance(1 person), and combined disturbance(1 person).

물표면채집기를 이용한 부산지역의 대기건성침적특성

정장표,이승훈 慶星大學校 環境問題硏究所 1998 環境硏報 Vol.8 No.1

Atmospheric dry deposition involves the transport and removal of aerosols and gases from atmosphere onto surfaces in the absence of precipitation, which is an important mechanism in controlling the fate of airborne toxic pollutants and their movements from the atmosphere to natural surfaces. In spite of increasing awareness of the importance of dry deposition in the fate of airborne pollutants, many uncertainties exist on the methods for calculation and measurements of dry deposition. One of the most pervasive problems in studies of air toxics is our inability to adequately measure or model dry deposition. The establishment of monitoring methodology and the evaluation of dry deposition characteristics that can accurately measure and estimate the dry deposition of airborne toxics to water bodies is imperative to prevent water bodies from being contanimated and to establish the management policies of water quality conservation. In this study, the flux of sulfate(SO??), nitrate(NO??) and chloride Cl?? measured in Pusan were (28.77. 21.86), (8.85, 5.18), (4.53, 2.69) ㎎/㎡/day, respectively. In addition the ratios of daytime fluxes to nighttime fluxes were 1.32, 1.71 and 1.68. From the above results, the fluxes of daytime showed higer than those of nighttime for all three acidic aerosols. 2. By the results of the differences in fluxes of SO?? and NO?? by the sample sites, the total average fluxes of SO?? in Chicago, Seoul and Pusan were 24.15, 21.50, 25.32 ㎎/㎡/day respectively, which showed similar flux levels. And the flux of NO?? in Pusan was 6.39 ㎎/㎡/day which was higher than 5.08 ㎎/㎡/day in Seoul. 3. The particle size distribution analysis for the samples of water surface showed that the particle size ranged from 30 ㎛ to 400 ㎛ and the particle distribution curve showed typically bell type of one peak. But the particle distribution curve was irregular type of multi-peaks and the particle size ranged from sub-micron to 400 ㎛ for the samples of dry deposition plate.

다발성 동맥류와 혈전증을 동반한 베체트병 1예

신표진,김장영,이승환,윤정한 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

부산시 자동측정망자료의 Weibull 확률분포함수 적용 및 기준치 적합성평가

정장표,이승훈 경성대학교 공학기술연구소 1998 공학기술연구지 Vol.5 No.-

The urban air quality in Korea has been deteriorated due to the rapid urbanization, industrialization and sudden increase of traffic volume. To scope with the air pollution problems, we need to establish the effective air quality management strategies based on evaluating the status of pollution and characteristics of concentration through the continuous monitoring. Also, We need to not only assess the degree of air pollution accurately based on the ambient air quality standards, but also check out whether the ambient air quality standards were properly established. From this study, it was obtained that concentration distribution for the criteria-pollutants measured at eight-air monitoring networks in Pusan area in 1997, was well fitted to Weibull probability distribution function. And the degree of differences from air quality standards and the expected values obtained from Weibull concentration distribution function were in order of CO(0.83∼0.90)〉SO₂(0.45∼0.80)〉TSP(0.24∼0.53)〉NO₂(0.23∼0.45)〉O₃(0.11∼0.30) in Pusan Area.