매실박, 당근박, 감귤박 급여가 산란계 난황의 Vitamin E, K 함량에 미치는 영향

최승현,나재천,권오상,박상미,이봉덕,안길환 충남대학교 농업과학연구소 2008 농업과학연구 Vol.35 No.2

This experiment is a quantitative experiment where insoluble Vitamins E and K are extracted from the yolk of the spawning which was fed by by-products of tangerine, carrot, or Japanese apricot. Finally the purpose is to raise contents of the insoluble Vitamins E and K inside the yolk of the spawning. The saponification method through α, α - dipyridyl was used to determine the content of Vitamin E. Diethyl dithiocarbamate assay was used to examine the content of Vitamin K. The contents of Vitamin E in egg yolks of chicken fed by by-products of tangerine, carrot, or Japanese apricot increased 2 - 3 times compared to control egg, while the contents of Vitamin K almost no changed. 본 실험에서는 감귤, 매실, 당근의 껍데기로 감귤박, 매실박, 당근박을 먹여 산란계의 난황에 들어있는 Vitamin E와 K 함량을 높이고자 했다. Vitamin E 추출방법에는 검화방법인 α, α -dipyridyl에 의한 비색정량법을 사용하였으며 전처리 과정 중 석유 ether를 가하여 층을 분리하는 과정과 증류수를 가하여 층을 분리하는 과정에 있어서 신중을 가하였고 신속한 시료 전처리과정이 요구되었다. Vitamin K 추출에는 Diethyl dithiocarbamate에 의한 방법이 사용되었다. 실험결과 일반난황에서의 Vitamin E 함량보다 실험난황에서의 Vitamin E 함량이 2배 이상 높아졌음을 알 수 있었던 반면 Vitamin K의 함량은소량 증가하여 큰 변화가 없었다. 위의 결과를 통해서 난황에 이전된 Vitamin E의 종류가 무엇이며 어떠한 형태의 Vitamin E가 이전이 많이 되었는지를 확인하는 실험이 필요할 것이다. 이러한 추가적인 실험이 이루어졌을 때 상업적으로 이용한 가치가 있는 가를 고려하여야 할 것이다.

한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

직접메탄을 연료전지용 귀금속 산화극 대체촉매의 개발

최재식,윤봉국,하승백,정원섭,이호인 한국공업화학회 2003 응용화학 Vol.7 No.2

Direct Methanol Fuel Cell (DMFC) is based on the technology of Polymer Electrolyte Membrane Fuel Cell. DMFC has several features of environmentally benign generation of electricity and high mobility for portable electronics etc., but its main problem to overcome for commercialization is the high cost of noble metal catalysts. In this study, it was found that the Pt/C catalyst supported on carbon nano fiber had the highest catalytic activity among the catalysts supported on several carbons. The newly developed Pt/C alternative catalyst had higher catalytic activity for methanol oxidation than the Pt/C catalyst supported on Vulcan XC-72.

교량 받침의 지지조건에 따른 강상자형 교량의 거동에 관한 연구

최승화,권오봉 三陟大學校 産業科學技術硏究所 2002 産業科學技術硏究論文集 Vol.7 No.1

21C 신지식인 양성을 위한 EQM 시스템 구축

최병태,이형민,전상봉,서승교,이우언,백종현 대구산업정보대학 2001 논문집 Vol.15 No.-

The aim of this paper is to form and to operate EQM, to say education quality management. In order to achieve the above stated purpose, the paper hypothesizes that there are similarities between enterprises and colleges in quality management. The basic method of the paper is systematic approach, modifies TQM, which is total quality management. The result is that EQM program must form and operate the feedback system for teaching and learning effect, the PSMT(project, self-study, media, team unit style teaching and learning) as a new teaching and learning method, and the digital system as an advanced mechanism, in education quality management.

호기성 미생물을 이용한 크롬의 생물학적 환원

최성우,이봉섭,곽명화,윤철종,박승조 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.2

Chromium occurs naturally in the earth's crust as well as in air, surface, and ground water. However, chromium is released to the environment in much larger amounts as a result of human activities. Hexavalent chromium, Cr(Ⅵ), is very soluble in water in natural environment and very toxic, carcinogenic, and mutagenic in animal and man. On the other hand trivalent chromium, Cr(Ⅲ), is less toxic and less soluble and thus is a lesser problem. This study has been carried out in order to reduce toxicity of Cr(Ⅵ) by an Escherichia coli strain. After adding E. Coli., the results were obtained from concentration of total Cr, Cr(Ⅵ). Cr(Ⅲ), pH, turbidity, and VSS in aerobic condition was summarized as follows : After 48hr aerobic condition in experiment of synthetic wastewater, the reduction rate of Cr(Ⅵ) was 20%. Also, the reduction rate of Cr(Ⅵ) was 60% in experiment of raw wastewater.

프레탈^(R)정(실로스타졸 50mg)에 대한 실로졸^(R)정의 생물학적 동등성

최한곤,권기철,이승호,김학미,박병주,유봉규,이종달,이경희,하정희,우종수,박인숙,최진석,용철순 한국병원약사회 2003 병원약사회지 Vol.20 No.1

Bioequivalence of two cilostazol tablets, the Pletaal^(R)(Korea Otsuka Pharmaceutical Co., Ltd.) and the Cilozol^(R)(Hanmi Pharmaceutical Co., Ltd.), was evaluated according to the guideline of KFDA, Sixteen normal male volunteers(age 20~29 years old) were divided into two groups and a randomized 22 cross-over study was employed. After two tablets containing 50㎎ of cilostazol were orally administered. blood was taken at predetermined time intervals and the concentration of cilostazol in plasma was determined with an HPLC method using UV detector. The pharmacokinetic parameters(C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters. The results showed that the differences in C_(max), T_(max) and AUC_(t) between two tablets were 4.99%, 1.74% and 7.68%, respectively. The powers(1-β) for C_(max), T_(max) and AUC_(t) were83.92%, 80.12% and 85.03%, respectively. Detectable differences(Δ) and confidence intervals were all less than 20%, and confidence interval of all the parameters were also less than 20% at the significance level(α) of 0.05. All of these parameters met the criteria of KFDA for bioequivalence, indicating that Cilozol^(R) tablet is bioequivalent to Pletaal^(R) tablet.

중년기의 스포츠 체력특성에 관한 연구

鄭升溶,崔淙洙,李鍾珏,金昌範,長鳳愚 忠北大學校 平生體育硏究所 1990 平生體育硏究所 論文集 Vol.4 No.-

The study aims to have cognizance of middle-aged mental and physiological properties by means of presenting the feature of physical strength, and to make middle-aged people try to prevent symptoms of senility and live a healthy and beneficial life for the rest of their days by means of showing a program of physical exercise suitable for middle-aged people. According to the middle-aged feature of physical strength, the decline of physical strength and physilogical function was remarkable. Individual variation in physical strength was wide and bodily organization was likely to get damaged easily as one attains an advanced age. Middle-aged people was slower in revovering and blood pressure was higher than young one after the same physical exercise. In conclusion, middle-aged people must build up his physical strength and try to prevent symptoms of senility through light physical exercises rather than heavy ones.

뇌하수체 선종내 출혈의 임상분석

이봉훈,김오룡,김성호,배장호,최병연,조수호 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.5

人體에서 分離한 葡萄糖 非醱酵菌의 抗菌劑 耐性에 關한 硏究

李淵台,崔承求,朴哲熙,曺圭鳳 단국대학교 대학원 1991 學術論叢 Vol.15 No.-

This experiment was conducted to classify for the 82 strains of glucose nonfermentative bacteria obtained from K hospital, in 1988, on the biological differentials and to test the resistance reaction on the drugs for those strains. The results were as follows : 1. Eighty two strains were classified into 65 strains of 79.3% of Ps. aeruginosa, 10 strains of 12.2% of Ps. cepacia, and 7 strains of 8.5% of Ac. calcoaceticus as results of 0 F sugar test, oxidase test, and DNase test. 2. It showed that Ps. aeruginosa was coincided with AP, CM, GM, KM, CF, and TC from the disk and MIC methods in the test of resistance reaction to antimicrobial agent, and especially, that AK and TOB KM and GM were observed high resistance reactive from MIC method. Resistnace to antibiotics for Ps. cepacia showed the identical reaction to AP, CB, CM, AK, SPT, CF, TC, TOB, KM and GM were observed high resistance reative from MIC method. Ac. calcoaceticus was identified the same resistance reaction to antibiotics to CB, KM, GM, AK, CF, and TOB, AP, SPT and TC showed high resistance reaction from the disk method. 3. CF and AP were observed the highest resistance reactions among 10 kinds of drugs used in this experiment and following was CM, KM and TC in order. 4. Ps. aeruginosa, Ps. cepacia, and Ac. calcoaeticus showed all multiple resistance reactions in the test of resistance to drugs, and Ps. aeruginosa was identified high resistance reaction to 6 drugs in 24.6%, Ps. cepacia was 9 drugs in 60%, and Ac. calcoaceticus showed resistance reaction to various kinds of drugs. 5. It showed important question in result of these studies that resistance percentage of NFB decreased by optimal antimicrobial selection and effective treatment established from serious antimicrobial treatment.