Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

Bioceramics 종합공영균이 과채류 생육에 미치는 영향

박평수,채윤석,최황 진주산업대학교 농업기술연구소 1997 農業技術硏究所報 Vol.10 No.-

Bioceramics 종합공영균양액의 처리가 오이의 생육 및 수량, 방울토마토와 부강고추의 생육에 미치는 영향을 연구하여 얻은 결과는 다음과 같다. Bioceramics 양액 시여구에는 대조구에 비해 오이, 방울토마토 및 부강고추의 엽수, 초장, 경경, 근장, 전체중량, 엽장 및 엽폭이 양호한 편으로 건강한 식물체를 생산할 수 있었고, 오이의 수확량도 상당히 많은 증수의 결과를 얻었다. 향후 Bioceramics 양액의 시여가 토양에 미치는 영향과 양액의 조성 및 시여방법 등에 관한 보다 세밀한 연구가 필요할 것으로 사료된다. This study was carried out to find the effect of bioceramic contained complex microorganisms on the growth and yields of cucumber, small bell type tomato and pepper plants. The number of the leaf, length of the plant and the root, total weight, the length and width of the leaf of treated plants had shown their significant effects than those of nontreated plants. Bioceramic solution produeced healthy plants and the higher yields especially in cucumber plants. It is required futher study for soil effectiness, the composition and applying methods of Bioceramic fertilizer.

로자탄 대 포시노프릴의 고혈압 환자들에서 항고혈압 효과, 안전성 및 내약성의 비교

박대균,이명묵,채인호,이무용,이홍자,김효수,김철호,손대원,오병희,박영배,최윤식 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.1

특수 언어발달 장애 환아의 임상 및음향음성학적 특성연구

김연희,박세훈,신용일,김찬양,김현기,김정수 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.1

Cone-beam CT를 이용한 안면비대칭자에서 이부편위에 따른 치성보상의 양상분석

박수병,박정희,정연화,조봉혜,김용일 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.5

Objective: The purpose of this study was to evaluate the correlation between menton deviation and dental compensation in facial asymmetry. Methods: Tooth axis and distance of first molar and canine to the reference plane were investigated by cone-beam computerized tomography. The subjects consisted of 50 patients with asymmetric mandibles (male 21, female 29, mean age 24.3 years). Control groups were also assessed (male 11, female 9, mean age 25.6 years). Nine measurements (5 linear measurements and 4 angular measurements) were measured in order to evaluate the correlation between menton deviation and the linear and angular difference of first molar and canine in the deviated and none-deviated sides using the defined MPR images. The differences between deviated and non-deviated side, according to menton deviation, were statistically analyzed using stepwise multiple regression analysis. Results: From the result, Menton deviation was negatively correlated with mandibular first molar's angular measurement (Δ∠LM6-Mn plane (dev.-ndev.)) and positively with maxillary fist molar's angular measurement (Δ∠UM6-FH plane (dev.-ndev.)) (p < 0.01). Two angular measurements (Δ∠ LM6-Mn plane (dev.-ndev.), Δ∠UM6-FH plane (dev.-ndev.)) explained the variability in menton deviation with a significant r² value of 0.589. Conclusions: This study suggests that the tooth axis of upper and lower first molars leans towards the deviated side of Menton when there is mandibular asymmetry with Menton deviation. 본 연구는 Cone-beam CT의 MPR이미지상에서 하악골 평면에 대한 하악 제1대구치와 견치의 편위측과 비편위측의 높이와 각도차이 그리고 FH 평면에 대한 상악 제1대구치와 견치의 편위측과 비편위측의 높이와 각도차이를 계측함으로써 안면비대칭자에서 이부편위에 따른 편위측과 비편위측의 제1대구치와 견치의 높이와 협설측 경사도를 CBCT를 이용하여 분석하고 그 관련성을 알아보는 데 있다. 연구대상은 하악골의 이부편위를 동반한 안면비대칭 환자 50명(여자 29명, 남자 21명, 평균나이 24세 4개월)으로 하였다. 대조군으로 평균나이 25세 7개월인 20명(남자 11명, 여자 9명)을 분석하였다. 결과는 이부편위에 대해 FH 평면에 대한 상악 제1대구치의 편위측과 비편위측의 길이 및 각도 차이(편위측 - 비편위측)는 대조군에 대해 통계적으로 유의한 차이가 존재하였다 (p < 0.01). 비대칭군에서 상악 제1대구치 각도는 편위측이 비편위측보다 평균 8.62 ± 5.95˚ 컸다 (p < 0.01). 상악 견치의 경우에도 편위측과 비편위측의 각도차이가 평균 4.48 ± 5.05˚로 통계적으로 유의한 차이가 존재하였다 (p < 0.01). 하악골 평면에 대한 하악 제1대구치의 수직 길이와 각도는 편위측과 비편위측에서 통계적으로 유의할 만한 차이가 존재하였다 (p < 0.01). 각도의 차이는 평균 -11.92 ± 5.79˚로 나타났다. 이부편위에 대한 상하악 제1대구치와 견치의 수직거리 및 각도의 Pearson 상관분석 및 회귀분석 결과, FH 평면에 대한 상악 제1대구치의 편위측과 비편위측의 각도차이(Δ∠ UM6-FH plane (dev.-ndev.))는 통계적으로 유의한 양의 상관관계를 나타내었고, 하악골 평면에 대한 하악 제1대구치의 편위측과 비편위측의 각도차이(Δ∠LM6-Mn plane (dev.-ndev.))는 통계적으로 유의한 음의 상관관계를 나타내었다. 이상의 결과에서 이부편위를 동반한 하악골 비대칭이 존재하는 경우, 이부가 편위되는 방향으로 상하악 제1대구치의 치아장축의 각도가 변화되는 것으로 나타났다.

동물의 장에서 분리한 Enterococcus sp.의 특성 및 분말화

박종진,변정수,조윤경,홍승서,이현수 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

의약품 및 동물약품으로 사용되고 있는 분말유산균을 개발하고자 동물의 장에서 산성 pH 및 담즙산에 대한 내성이 강하고 대장균 생육억제력이 좋은 Enterococcus faecium L20을 분리 동정하고 이를 이용하여 고농도의 생균이 함유된 분말 유산균을 제조하였다. 분리된 균주는 pH 3.0에서 90% 이상의 내성과 0.3% 담즙산이 함유된 배지에서 100%의 내성을 나타냈으며 MRS 배지에서 대장균과 혼합배양시 24시간 이내에 대장균을 사멸시켰다. 분리된 균주를 산업용배지에서 배양한 후 동결건조시켜 분말을 만들었을 때 생균수는 5.0×0^11/g 이상이었다. 이것은 18℃에 보관하였을 경우 11개월 동안 80%의 생존율을 나타내었다. In order to develop a lactic acid bacterial powder which can be used as a probiotic for human and animal, a lactic acid bacteria which has high resistance against low pH and ox-gall, and shows a good growth inhibition against low pH and ox-gall, and shows a good growth inhibition against E. coli, was isolated from an animal intestine and characterized. The isolated strain was identified as Enterococcus faecium. It had more than 90% of survival at low pH for 2 hours and almost 100% of survival in the presence of 0.3% ox-gall. When co-cultured with E. coli in MRS broth, all of the E. coli cells were killed within 24 hours. The final powdered product of the isolated strain was manufactured after a freeze drying process suing an industrial media, and then checked its stability. Its storage stability was 80% for 11 months at 18℃.

산지하천의 강우특성에 의한 부유물질 유출에 관한 연구

윤인규,박수진,최한규 강원대학교 산업기술연구소 2007 産業技術硏究 Vol.27 No.A

In this research, we have selected the region of Jawoon river that is the area of high land vegetable growing in the upper Soyang Reservoir and we have observed actual floating materials that generate negative nutrition and turbidity of the Reservoir water and the changes of water quality by raining of each month for one year of 2005 in order to monitor the relationship of pollution sources by the outflow of rain water. In addition, we also have conducted statistical inspection methods such as correlation analysis and regression analysis on strength of raining force and rain continuance time among the elements affecting the outflow of floating materials.

藥鍼用蜂毒液이 黑色腫細胞에 미치는 抗癌效果에 對한 分子生物學的 硏究

朴贊烈,南相水,金昌煥,李栽東,姜成吉,李潤浩,安秉哲 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability, apoptosis, and cell cycle were analyzed using MTT assay, tryphan blue assay, [3H]thymidnine release assay, flow cytometric analysis, activity of caspase-3 protease activity assay, and immunocytometric analysis of PCNA. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis- and cell cycle-related genes was performed. The obtained results are summarized as follows: 1.The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2.Significant induction of apoptosis was identified using tryphan blue assay. [³H]thymidine release assay, and flow cytometric analysis of sub G₁fraction. 3.In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4.Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-X□ were down-regulated whereas Bax was up-regulated by bee venom treatment. 5.In flow cytometric analysis of cell cycle and immunocytometric analysis of PCNA expression, cell numbers of G₁phase was increased by a dose-dependant manner. 6.In quantitative RT-PCR analysis of the cell cycle-related genes, p21, p27, and p57 were increased, while Cyclin D1, CDK4, c-Myc, c-Fos, and Histone H3 were decreased. In contrast, there were no remarkable changes in expression levels of CDC2 and c-Jun.

여성 하부요관석환자에서 무마취하 요관경하 배석술

박영호,송윤섭,임용순,김영호,이남규,구자현,서병욱,김민의,전윤수 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Although ureteroscopy(URS) has been used widely to remove ureteral calculi, there is an argument against the belief that anesthesia or admission is needed for URS. We evaluated the experience of URS without anesthesia in female patients. From September 1997 to July 1998, URS was performed in 31 female patients without anesthesia. EHL(Electrohydraulic Lithotripsy) was used in 21 patients with impacted or large stone over 5mm. All patients were given oral antibiotics for 3 days and follow-up KUB was taken to confirm successful removal of the stone one week after the procedure. Successful treatment was defined as complete removal of the stone or residual fragments less than 2mm. Overall success rate was 96.7%(30 cases among 31 cases). All but one patient tolerated with analgesia through the entire procedures. There were only minimal complications such as mild flank pain and hematuria. So, we recommend that non-anesthetic ureteroscopic removal of stone as the first choice for treatment of lower ureteral stones in female based on the minimal morbidity, high success rate and low cost.

GLE의 영어 교과과정

박상수,정윤걸 釜山外國語大學校 語文學硏究所 1999 外大語文論集 Vol.14 No.-

The students who study in GLE(=Globalized and Localized experts) program must have a good command of English, especially in speaking and listening when they complete whole courses. In order to achieve this aim successfully the English curriculum for the GLE should be organized systematically as well as the intellectual faculties of the students, teaching methods and the beginning age of English teaming should be considered seriously We researched the learning theories of a foreign language and the four functional skills of language and proposed a few hints that the students in the GLE keep in mind while they learn English. The results obtained from this paper are summarized as follows: 1. The students who study in the GLE can effectively improve the ability of the command of English not through the way of language acquisition but through the way of language learning because they began to learn English at middle school days. In fact at the age of over 12 or 13 the language acquisition faculty has grown and consequently the value of linguistic parameters has already been set. 2. When the students in the GLE learn English, the emphasis should be laid on the improvement of speaking and listening ability primarily and then reading and writing ability should be developed. 3. It is necessary that the students understand the British and American social and cultural properties fully. As a particular language is the product of the social and cultural life of a certain language group, language learning always includes the understanding of the social and cultural properties of the people who use the target language.