제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

원발성 폐암 환자의 생존율에 관한 보고

김현태,이상무,어수택,박춘식,정성환,허승재,남충희,강창희,김용훈 순천향대학교 1994 논문집 Vol.17 No.4

We analysed 404 patients with primary lung carcinoma who were treated at Soonchunhyang University Hospital from July, 1985 to september, 1993 in order to investigate the survival rate and epidemiolgical properties of primary lung cancer. They were 330 males and 74 females. The most prevalent decade was seventh. In terms of cell type, the squamous cell was 225 patients (55%), and adenocarcinoma, small cell, mixed type was 21%, 19%, 4%, respectively. Among non-small cell lung carcinoma, stage Ⅲa was the most prevalent one(92%). In case of small cell carcinoma, the limited stage was 64%. The 12-, 24-, 36- month survival rate of total patients was 57%, 31%, 22%, respectivley and median sruvival time was 15 months. The 36-month survival rate tended to be longer in non-small cell lung carcinoma than that of small cell lung cancer, but there was no difference between two groups, statistically. In non-small cell carcinoma, The 36-month survival rate and meidan survival time were longer in the stage Ⅰ and Ⅱ than those of Ⅲa, Ⅲb, Ⅳ (80% versus 38%, 22%, 0%, p<0.05). According to involvement of lymph node, the 36-month survival rate was longer in NO and N1 than those of N2, N3 (61.9%, 48.7% versus 17.7%, 17.3%, p<0.05). In small cell carcinoma, The 36-month survival rate and median survival rate were higher and longer in limited stage than those of extensive stage(16.1% and 13 month vs 10% and 8 month, p<0.05). In conclusion, we report here the incidence of primary lung carcinoma and the survival rate of paients with primary lung carcinoma who were treated in Soonchunhyang University Hospital.

위에 발생한 유암종 (Carcinoid Tumor) : 1예 보고

김남재,김진희,이경태,서광식,정현용,이승민,김석현,이병석,이헌영 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.3

A carcinoid tumor of the stomach is uncommon, has no clinical symptoms, and is regarded as a benign. It is also incidentally found in most cases. 48-year-old woman with gastric carcinoid tumor was admitted. She had suffered from a anorexia and a dry mouth for 4-months. A gastroscopy revealed a polypoid mass on the greater curvature of the mid-body of the stomach which was subsequently thought to be an adenocarcinoma. An endoscopic mucosectomy revealed however, that it was a carcinoid tumor. A case of carcinoid tumor of the stomach is here by presented with a brief literature review.

모더나이트형 제올라이트 촉매상에서 탄화수소에 의한 NO 제거반응

김문현,오승훈,정성엽,남인식,김영걸 전남대학교 촉매연구소 1998 촉매학술발표회 Vol.- No.15

태권도 수련이 삶의 질과 정신건강에 미치는 영향

이한경,남승현,김석일 龍仁大學校 武道硏究所 1997 武道硏究所誌 Vol.8 No.2

The purpose of this research was to analyze the mental status of a Taekwondo participant and a non-participant. The following were the results. 1) The analysis of Taekwondo participant´s relationship to life and quality were satisfacy at job, leisure, daily life, and steady manage. Consistency in Taekwondo training reduces job stress and physical fatigueness. 2) Taekwondo participant, who trains frequently finds quality of life more satisfying as a hobby and a genuine effect of the art. 3) Taekwondo participant, who trains consistently in one´s qality of life can attain affirnation of positive effects, depending on the individual. 4) In Taekwondo training, there are 9 mental health existence and non-existence. Sometization Obsessive-compulsive, Interpersonal-sensitive, Anxiety, Hastility. Phobic anxiety are all positively effective through Taekwondo training, However, the rest of the following fectors (Depression, paranoid ideation, Dsychoticism) are not effcetive.

전자 의료 카드를 위한 보건의료 정보 표준화 연구

박흥식,남승곤,안재억,김현의,김주한,정철규,김태진,이기한 대한의료정보학회 1998 Healthcare Informatics Research Vol.4 No.2

응급의료정보 체계를 위한 전지 의료 카드의 활용방안

박흥식,남승곤,안재억,김현의,김주한,정철규,김태진,이기한 대한의료정보학회 1998 Healthcare Informatics Research Vol.4 No.2

스포츠 센터 평생 회원들의 체련장 이용 실태에 관한 연구

이한경,유호길,김미정,김석일,남승현 龍仁大學校 1998 용인대학교 논문집 Vol.15 No.-

The purpose of this study is to analyze privileged mass' use of the sport for all facilities. The subject was members of five in hotel, who answered a questionnaire. The main results of this study are as follows : 1. It was found that comsumers of fitness center were at 40-50 age, and the mean of using period is longer than 3 years. 2. The membership card motivated members to improve, their health and in the older members losed self-health condition. 3. The members were used lower at facilities less. The mean of using frequency is one per four days. 4. It was found that sauna room was the most utilized facilities in fitness center. 5. It was fecund that training room was utilized less, because of lack of time. 6. The participant of training room not showed lower facility use at higher age. 7. The participator of trainning room did showed satisfaction for instructor and facilities. As started above, the turn of recognition which answers the purpose is needed for a privileged mass' use of facilities for sports for all, and a positive motivation of the managements of commercial sports center must be prepared indispensably. Also, a consideration for a resasonable and step by step remedy for all age groups is deeply demanded.

New records of the genus Chroomonas and two Chroomonas species (Cryptomonadales, Cryptophyceae) from Korean freshwater

Hyeon Ju Nam,Miran Kim,Seok Won Jang,Bok Yeon Jo,Eunyoung Moon,Seung Won Nam 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.4

The genus Chroomonas is a group of blue-green colored cryptomonads. This study describes two freshwater Chroomonas species for the first time in South Korea: Chroomonas nordstedtii Hansgirg and Chroomonas coerulea (Geitler) Skuja. We examined the morphology and ultrastructure of these species by light microscopy, scanning electron microscopy, and transmission electron microscopy. These two Chroomonas species were blue-green colored and ovate to oval-shaped. Chroomonas nordstedtii was characterized by two Maupas ovals with hexagonal periplast plates, whereas C. coerulea was characterized by one eyespot with rectangular periplast plates. A molecular phylogeny with data from nuclear SSU rRNA and chloroplast rbcL genes revealed that Korean C. nordstedtii formed a distinct clade with NIES-708, NIES-1004 from Japan, and UTEX 2779 from Colorado, USA, while C. coerulea formed a clade with ACOI 1366 from Portugal.

실물옵션 접근법을 활용한 ASP 아웃소싱 리스크 관리

남승현(Nam, Seung-Hyeon) 한국산학기술학회 2010 한국산학기술학회논문지 Vol.11 No.7

본 연구에서는 정보시스템 아웃소싱 프로젝트의 성공을 위하여 실물옵션접근법을 활용하여 아웃소싱 프로젝 트를 수행할 때 발생할 수 있는 위험을 효과적으로 관리하는 방법에 대하여 살펴보고자 한다. 지금까지 대부분의 정 보시스템 아웃소싱 프로젝트 관련 연구는 핵심성공요인 또는 프로젝트의 성과 측정에 맞추어져 주로 수행되어 오고 있다. 정보시스템 아웃소싱 프로젝트의 대표적인 형태인 ASP(Application Service Provider)에 대하여 아웃소싱 프로젝 트를 수행하는 도중에 발생 가능한 여러 가지 유형의 위험을 정의하고 이 위험의 발생원천을 살펴봄으로써 아웃소싱 프로젝트의 성공을 위하여 관리 가능한 위험요인들을 실물옵션접근법을 이용하여 효과적으로 관리하는 방법을 제시 하였다. Information System (IS) Outsourcing is one of the powerful alternatives to guarantee the competitive advantages in the rapidly changing business environment. Most studies on IS outsourcing area have focused on the Critical Success Factors(CSFs) influencing the Success of IS outsourcing or the performance measuring the success of IS outsourcing projects. The CSFs or the performances which measures the success of IS outsourcing project have limitations to explain how to succeed in doing IS outsourcing project. This study uses Real Options Approach to make IS outsourcing projects' success by recognizing and minimizing IS outsourcing risks.