물리ㆍ화학적 처리에 의한 멸균 초콜릿 우유 오염균의 생육억제 효과

최문경(Moon-Kyoung Choi),윤소영(So-Young Yoon),이소영(So-Young Lee),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),정지연(Ji-Yeon Jung),곽지희(Ji-Hee Kwak),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),이주운(Ju-W 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.8

멸균 초콜릿 우유로부터 분리한 내열성 균주에 대해 열, pH, 전해수, 오존처리, microwave 및 감마선 처리를 하여 균주의 사멸효과에 대해 알아보았다. 균주의 지방산 분석과 API kit를 통하여 균주를 동정한 결과, Bacillus lentus로 동정되었으며, 잠정적으로 Bacillus lentus M1으로 명명하였다. B. lentus M1에 110℃, 15분간 열처리하였을 경우 생육이 억제되었으며, pH 처리 시 pH 5 이하, 10 이상에서 생육이 억제된 것으로 나타났다. B. lentus M1에 대한 전해수의 항균활성을 paper disc법으로 측정한 결과, 높은 생육억제를 보였으며, 오존 처리의 경우 초기 균수가 10² CFU가량의 균을 10분 동안, 10³ CFU가량의 균을 30분 동안 처리 시 균의 생육이 억제되는 것으로 나타났다. Microwave를 1분간 처리 시 B. lentus M1이 모두 사멸한 것으로 나타났다. 감마선 조사의 경우, 1 kGy 조사 시 생균수가 1.61×10³ CFU로 초기 균수에 비해 4 log cycle 가량 균수가 감소하였으며 7 kGy에서 완전히 사멸하였다. 이상의 결과를 통해 열, pH, 전해수, 오존 처리 및 방사선 처리 방법이 멸균 초콜릿 우유의 생존 오염균인 B. lentus M1을 효과적으로 사멸시킬 수 있을 것으로 사료된다. This study was conducted to investigate the cause of microbiological contaminants in aseptic chocolate milk and evaluate the effect of a physicochemical treatment on the growth inhibition of isolated bacterial strains. The bacterium isolated from aseptic chocolate milk was identified as Bacillus lentus and was named B. lentus M1. In the heat and pH treatment, the growth of B. lentus was inhibited at 110℃ for >15 min and at pH’s <5 and >10. An electrolyzed water treatment against B. lentus M1, revealed 5 ㎜ growth past the inhibition zone. The effect of ozone gas on B. lentus M1 growth was evaluated using viable cell counts. When the initial number of B. lentus M1 was 10² and 10³ CFU, the bacteria were completely suppressed by ozone gas treatment for 10 and 30 min, respectively. In a microwave treatment, B. lentus M1 was sterilized following microwave treatment for 1 min. As the result of γ-irradiation against B. lentus M1, numbers decreased as the γ-irradiation dosage increased. These results show the growth inhibition effects against contaminants in aseptic chocolate milk using physicochemical treatments.

상용 에폭시 봉지제의 경화과정의 유변특성

文仁植,吳明淑,金珉珠 弘益大學校 科學技術硏究所 1998 科學技術硏究論文集 Vol.9 No.2

스모그 챔버에서 수분 반응에 의한 대기 에어로졸의 생성 및 성장

김민철,배귀남,문길주,박주연 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.2

Aerosol formation and growth by water vapor reactions were investigated in a 2.5-㎥ indoor smog chamber filled with the unfiltered ambient air. The relative humidity of test ambient air was elevated at 59~64% or 84~88% by adding water vapor. The aerosol number size distribution and the concentrations of O₃, NO, NO₂, and SO₂ were measured during the experiments. The O₃, and NO₂ gases were well reacted with the water vapor at high relative humidity of 84~88%, and the reaction rates of these gases seemed to be decreased at low relative humidity of 59~64%. The formation and condensational growth phenomena of ambient aerosols by water vapor reactions were observed in a Teflon bag, depending strongly on the initial particle size distribution. The water vapor reactions might be affected by the contents of oxidants produced by photochemical reactions under sunlight.

일부농촌지역의 농약사용실태 및 농약중독 요인 조사

이경민,송주희,장재혁,심수정,강양화,안재경,이숭호,박미영,정문호 대한보건협회 2002 대한보건연구 Vol.28 No.4

Object The usage of agricultural chemical is on the increasing. Use of pesticides has increased agricultural production. However, negative externalities from such use have increased too. Pesticide poisoning is a major public problem. The purpose if this study is to find out the relationship between keeping the safety rules or protective equipments or attending safety education or dose of pesticides use , and poisoning experience. Methods For Gu, H.M. and Pack, S.G. study , we obtained questionnaire data concerning pesticides poisoming. A person interviews on 203 were conducted in two villages in Sinmeari and woulbonri Chunchun , Kangwon province, July 2002. Result 1. 144 people(70.9%) have sprayed pesticides and the rata of experiences of pesticide poisoning was 26% among 144. Spraying time of pesticides was 1-2 hours in 63.9% of farmers. Rate of using the protective equipment was 25.7% (protective clothes), 49.3% (Mask), 48.6 (Gloves), 7.0 (Protective glasses), 64.6% (boots) respectively. 2. Chi-square and multiple logistic regression analysis showed that Duration of spraying per day (<2hr) and using of mask(protective equipment)are significantly association with poisoning experience.(x^2=5.2684 p=0.0217, CI=0.140-0.5853 OR=0.346 p=0.0211 respectively). But no association between poisoning experience and keeping safety rule, attending of safety education. 3. Spraying pesticides. Duration of spraying pesticides, reading manuals and no spraying at fatigue (keeping safety rule) are significantly association with symptom experience(x^2=14.0621 p=0.0002, x^2=7.0639 p=0.0079, CI01.395-10.950 OR=3.908 p=0.0095, CI=0.101-0.726 OR=0.271 0.0094 respectively). But no association between symptom experience and protective equipments, attending of safety education.

국내 공개소프트웨어의 도입 실태 및 활성화 장애요인에 관한 탐색적 연구

권문주,김태웅,김민하 한국정보사회진흥원 2008 정보화정책 Vol.15 No.4

공개 소프트웨어는 소스코드가 공개되어 있는 소프트웨어로서 누구나 자유롭게 활용·배포가 가능하며 비용이나성능 측면에서 전용소프트웨어에 비해 상당한 이점이 있다. 본 논문은 공개 소프트웨어 사용과 관련하여 503개 정부 및 교육기관 그리고 일반 기업에 대한 설문조사 결과를 제시하고 도입의 장애요인을 논의하는데 목적이 있다. 조사결과, 응답기관 및 기업의 62.6%가 최소 한 가지 이상의 공개 소프트웨어를 사용한다고 응답하였으며, 37.4%의 미사용 기관 및 기업들은 호환성(24.7%), 신뢰성(19.0%), 안정성(18.3%), 유지보수(15.3%), 사내 전문가 부재(12.3%) 등의 이유로 사용을 주저하고 있다. 또한 사용하다가 중단한 기관 및 기업들도 호환성(25.0%), 신뢰성과 유지보수(15.0%), 안정성과 사내 전문가 부재(18.3%) 등을 이유로 들고 있다. 더구나 사기업의 48.4%는 향후 공개 소프트웨어를 도입할 계획이 없다고 한다. 이와 같이 공개 소프트웨어 시장은 아직도 도입초기단계로서, 사유 소프트웨어에서 공개 소프트웨어 사용으로의 전환을 위해서는 정부지원이 절실한 상태이다. OSS(Open source software) pursues the freedom to have access to open source and offers several advantages, over the proprietary software, to enterprises. This paper presents a survey results from 503 companies and institutions concerning the use of OSS. 62.6% of these companies and institutions reported that they use at least one or more applications of OSS. But, for those 37.4% of companies and institutions, the most relevant obstacles for diffusing and adopting technological solutions based on OSS include the compatibility(24.7%), credibility(19.0%), stability(18.3%), maintenance(15.3%), no in-house expert(12.3%), etc. Many companies and institutions have also discontinued using OSS due to the problems related to compatibility(25.0%), credibility and maintenance issues(15.0%), stability and no in-house expert(18.3%), etc. Furthermore, 48.4% of the private companies have no future plans for adopting OSS. The results clearly show that the market for OSS is still in infant stage and desperately needs the government support to reduce the resistance to change from proprietary software to OSS.

GPS를 이용한 2차로 고속도로에서의 추월시거 산정방법에 관한 연구

임채문,김정민,박영환,이주호 大邱大學校 科學技術硏究所 2001 科學技術硏究 Vol.8 No.2

This study applied GPS(global positioning system) into the field of traffic and transportation, measured overtaking mechanism at two lane highway. After the analysis of the collected data from the measurement, it was constructed passing sight model which is based on AASHTO(american association of state highway and transportation officials) model and; as the result, passing sight distance was computed. To accomplish this goal, speed and position information for leading and following vehicles were recorded under actual highway conditions. Each vehicle's distance, speed, and acceleration characteristics were calcuated using the passing algorithm equations proposes. The computed value of acceleration was 1.37m/sec2. This value was twice as large as the value calculated earlier(0.6m/sec2). This study proposed 326m as passing sight distance at maximum speed(80km/hr) in 88 Olympic highway.

목포해역의 확산양상

강주환,박선중,문승록,박민원 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

Tidal currents had been reduced remarkably since the constructions of Youngsan River Seadike and Youngam/Keumho Seawalls at the semi-enclosed Mokpo coastal zone. This deteriorates the water quality of the zone seriously, because reduced currents prevent pollutants flushing out or diluting easily. Three cases are simulated numerically with HD and AD modules of the MIKE21 software; continuously pollutants discharging case at Mokpo Harbor, intermittent discharging case at the gate of the Seadike, and continuously pollutants discharging case at Chungkye Bay. The results of all the cases show that the majority of the pollutants does not flush out but remains at Mokpo coastal zone. Especially, the release from gates of the Seadike may cause that fresh water concentration prevails near Mokpo Harbor.

다량의 하부위장관출혈이 병발된 Vibrio vulnificus 패혈증 1예

최주연,유진홍,한석원,이대훈,최민호,박순민,김연식,김선우,신완식,강문원,강성구 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.2

최근 저자들은 Vibrio vulnificus 감염에 의해 패혈증 및 이차성 피부 병변을 보인 42세의 남자 환자의 치료중 하부 위장관 출혈이 병발된 1예를 경험하였기에 보고하는 바이다. We experienced a case of Vibrop vulnificus septicemia which showed an unusual manifestation such as hematochezia. The patient was a 42-year-old man with chronic liver disease. The blood and wound culture revealed the organism. During intensive care, he suffered from massive hematochezi for several days. Colonoscopy and abdominal CT showed findings suspicious of ischemic colitis. To our knowledge, this is the first unusual case of V.vulnificus infection which showed massive lower gastrointestinal bleeding.

프롤린 함량 증가 및 내건성 강화 관련 새로운 RING-Type E3 유비퀴틴 리가아제 애기장대 atrzf1 돌연변이체의 생리적 기능 연구

주현우,민지희,정문수,김철수 전남대학교 농업과학기술연구소 2021 농업생명과학연구동향 Vol.59 No.-

The covalent attachment of ubiquitin to proteins plays a fundamental role in the regulation of cellular function through biological events involving abiotic of biotic stress responses, immune responses, and apoptosis, Here, we characterize the biological function of the Arabidopsis thaliana RING Zinc Finger 1 (AtRZF1) in dehydration response. AtRZF1 was significantly reduced by drought stress. The atrzf1 mutant was less sensitive to osmotic stress than the wild-type during early seedling development, wheareas transgenic pants overexpressing AtRZF1 were hypersensitive, indicating that AtRZF1 megatively regulates drought-mediated control of early seedling developmeng. Moreover, the ectopic expression of the AtRZF1 gene was very significantly influential in drought sensitiv parameters including proline content, water loss, membrance ion leakage and the expression of dehy-dration stress-relat genes. ARZF1 is a functional E3 ubiquitin ligase, and its conserved C3H2C3-type RING domain is likely important for the biological function of AtRZF1 in drought response. Together, these results suggest that the E3 ligase AtRZF1 is an important regulator of water deficit stress during early seedling development.

영상처리시스템을 이용한 아크용접 로보트의 자기교시법

박민기,박민용,김문주 연세대학교 산업기술연구소 1993 논문집 Vol.25 No.2

In this paper, a new method of sensing the welding line of a workpiece is proposed. We use a CCD camera as the sensor and two-dimensional welding line of the workpiece is obtained by processing and scanning the image. Using the welding line and extended chain code, the information of welding line is obtained and stored, so the self-teaching is done. Finally, we prove that this self-teaching method is available to a welding robot by an experiment.