대황황련해독탕의 항고지혈증 작용

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2001 No.-

Whangryunhaedok-Tang (WT) is formulated with Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix and Gardeniae Fructus, and Daewhang-whangryunhaedok-Tang (DWT) is made by the combination of Rhei Rhizoma, a wellknown anticostipation drug in WT. Therefore, DWT has been evaluated for antihyperlipidemic effects on experimental hyperlipidemic rats and mice induced by corn oil and high cholesterol-diet. Oral administration of DWT significantly inhibited the increase of serum triglyceride and LDL-cholesterol levels, and the decrease of serum HDL-cholesterol levels in hyperlipidemic rats induced by corn oil. Also, oral administration of DWT significantly prevented the increase of serum total cholesterol, triglyceride and LDL-cholesterol, and liver total cholesterol and triglyceride in 1% cholesterol-diet fed mice. These results suggest that DWT is effective for the treatment of hyperlipidemia.

Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

대황황련해독탕의 사염화탄소 유발 간장해 보호효과 미치 급성독성

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2002 東西醫學硏究所 論文集 Vol.2002 No.-

ABSTRACT - This study was performed to evaluate hepatoprotective effect of daewhang-whangryunhaedok-Tang(DWT) on liver injured rats induced by CCI_4 and the acute oral toxicity of it in mice. The activities of serum transaminase(ALT/AST), alkaline phosphatase(ALP) and lactic dehydrogenase(LDH), the levels of serum total cholesterol(TC) and triglyceride(TG), change of liver enlargement, and inhibitory activities of lipid perotidation, catalase and glutathione-S-transfrease(GST) in liver microsome were determined in hepatotoxic rats induced by CCI_4. DWT was significantly reduced the serum ALT, AST, ALP, LDH. TC and TG lecels. And, the increase of lipid peroxidation, decrease of catalase and GST activities in the liver microsome of CCI_4-intoxicated rat were significantly improved by the treatment of DWT. Male and female mice were administered maximum dosages of 5.000 mg/kg b.w. of DWT. After single oral administration of DWT to mice, we observed them daily for 2 weeks.DWT did not induce any toxic signs in the mortalitie, clinical signs, body weight changes, and gross necropsy finfings of mice. Based in these results. It is concluded that DWT may have the hepatoprotective effect on CCI_4 induced hepatotoxicity in rats. Also. DWT may have no side effect and its LD_50 value may be over 5.000mg/kg b.w. in mice.

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

파생 사동사의 범주에 관하여

김형배 건국대학교 1995 대학원 학술논문집 Vol.42 No.-

The purpose of this study is to examine the category of causative verb in Korean. The notion of causative is that the causer operates on causee directly or indirectly and makes the causee come into a result. We can say that the grammatical causative is grammatical category to re-alize the causative. Also, causative verb is derived verb combined with causative suffix at the root of declinable word and has declarative function which causer makes the causee come into a result by operating directly or indirectly. We looked into category of causative verb in the view of morphological, syntactic, semantic. As a result, causative verb in Korean is the morphologically combined with causative suffix '-i-, -hi-, -li-, -ki-, -u-, -ku-, -chu-' at the root of declinable word, and syntactically must keep the case form in addition to the relationship with main verb in meaning.

세포학적인 검사를 통해 자궁경부암의 조기 발견과 합리적인 병원내 산부인과의 운영 체계에 관한 고찰

김태전,배형준,김종열 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

In this study, the authers have investigated a true appreciation on the importance and the actual conditions of utilization of cytologic examination in gynecologic services with 3 step, that is, in 1 step the importance of cytologic examination in gynecologic services has been investigated from the results of 4,024 female of cytologic examination, in 2 step the actual conditions of utilization of cytologic examination have been investigated from 60 medical institutes through questionaire, and in 3 step the more detailed subjects of cytologic examination in gynecologic services have been investigated from 3 medical institutes selected randomly, such an investigations were done to make the programs of improvement in order to promote the activation of gynecologic medical services that shall be able to decrease occurrence of uterine cervical cancer, which is still the most prevalent cancer in korean female, and to make those on effective diagnosis and treatment activaton that shall be able to increase the income of medical institutes. The programs of improvement according to conclusions obtained from investigations like these were summarized as the following. 1. Program to activate positively cytologic examination It was well known that good correlation was noted between number of cytologic examination and those of histologic examination, those of gynecology procedures, and those of gynecologic surgeries. But it was a fact that a most of gynecology board certified physicians and physicians, and medical institutes have not utilized actively cytologic examination in gynecologic practice. Therefore all of married woman should be had politically cytologic examination at regular intervals in order to prevent and detect early uterine cervical cancer. 2. Program to establish exclusively responsible managed systems Managed systems that are exclusively responsible for cytologic examination should be established in order to have examination at regular intervals exactly(six months). 3. Program to utilize correctly the current medical insurance regulations about medical treatment charges The medical charges of cytologic exmination should be applied correctly to the current medical insurance regulations about medical treatment charges in order to increase the income of medical institutes. Because the medical charges of cytologic examination for the levels of medical check up and preventive medicin exception cancer or clinically suspicious cancer diagnosed by physicians is applicable to general medical treatment charges under the current medical insurance regulations. 4. Program to utilize reasonable diagnostic system in cytologic examination. Since certificated technologist screen first thing many microscopic slides for cytologic examination, lastly gynecologic board should make a final diagnosis on only abnormal slides. It is reasonable diagnostic system that can observe more microscopic slides, decrease misdiagnosis and high personnel expenses, and increase income in cytologic examination. Consequently it is necerssary to establish reasonable diagnostic system. 5. Program in order to promote the efficiency of the following examination and treatment system for patients to whom abnormal cell were detected. The following examination and treatment system that were corresponded with diagnosis, and automated and standardized system from receipt to gynecologic practice should be established to offer patients ease and trust, and also medical institutes the more income through the activation of gynecologic practice. Finally, the authers think that it is very important that cytologic examination should be included in routine gynecologic practice.

충청지역 사육 돼지의 호흡기 및 소화기 병변으로부터 분리된 병원세균의 약제 감수성

김지영,김태용,장경수,김석,허지연,박배근,전무형 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

During the past two years(1999-2000), the antimicrobial susceptibility tests for the major pathogenic bacteria isolated from the respiratory or digestive organs of the diseased pigs in Chungchong area have been carried out. Total of 88 strains in seven species were isolated from the respiratory lesions of the diseased pigs during the periods, and the major isolates were in order of P. multocida(27.3%), Staphylococcus spp.(20.4%), Streptococcus spp.(15.9%), E. coli(12.5%), B. bronchiseptica(11.4%), Haemophilus spp.(10.2%) and A. pleuroneumoniae(2.3%). From the fecal specimens of the pigs with diarrhoea, total of 146 strains in five species were isolated in order of E. coli(59.6%), Salmonella spp.(17.8%), Campylobacter spp.(14.4%), Streptococcus spp.(5.5%) and Clostridium spp.(2.7%). The isolates were tested the susceptibility for 30 kinds of antibiotics. Among the isolates from the swine repiratory lesions, P. multocida revealed over 71% of susceptibility to ciprofloxacin(CIP), enrofloxacin(EF), tylosine(TL) and norfloxacin(NOR). B. bronchiseptica showed over 70% susceptibility to CIP, EF, linco-spectin(LS) and NOR, and Haemophilus spp. over 78% of susceptibility to AN, CIP, EF and NOR. The isolates of Streptococcus spp. revealed over 80% susceptibility to CIP and EF, and Staphylococcus spp. over 78% of high susceptibility to amikacin(AN), cephalosporin(CP) and CIP. .In general, most of isolates were highly resistant to ampicillin(AM), bacitracin(B), ceftiofer(CT), chlorampenicol(C), colistin(CL), erythromycin(E), nalidixic acid(NA), novobiocin(NB), oxacillin(OX), penicillin G(P), polymyxin B(PB) and vancomycin(VA). Of the isolates from the pigs with diarrhoea, over 76% of E. coli isolates showed the high susceptibility to AN, LS and N. Salmonella spp. revealed over 71% of susceptibility to CT, CIP, EF, LS and NOR, and Campylobacter spp., over 76% of susceptibility to CT ,CIP, EF, GM, LS and TL. Higher susceptibility were observed CIP, LS, NOR and TL with Streptococcus spp. and AN, CIP, EF and LS with Clostridium spp.. Most of the isolates showed the higher resistance to B, CL, L, N, NB, OX, P, PB Te and VA. The MIC range of the major isolates for AN, CIP, NOR, LS FM and GM showing the relatively high sensitivity were measured. Compared with other drugs, CIP and LS showed the lower MIC of ≤0.06∼128 ㎍/㎖ and ≤0.06∼≥512 ㎍/㎖, respectively.

읽기의 비형식적 평가 방법에 관하여

김형배 서울敎育大學校 初等敎育硏究所 1997 한국초등교육 Vol.9 No.1

현대국어 피동사 파생의 조건

김형배 건국대학교 1995 대학원 학술논문집 Vol.40 No.-

The purpose of this Study is to formulate the Phonological condition in a passive verb derivation. In Korean, the passive suffixes are occured a form such as'-i(이)~','-hi(히)-','-li(리)-'ard'-ki(기 )-'. The study is started from a questions which the choice of a suffix is aocomplished by what condition and rule. In the case of derivation of the passive verb in Modem Korean, each different form is combined by the phonological condition in the final phoneme of root. (A concltion of passive verb derivation in Modern Korean) 1.The final phoneme of mot is a vowel ; combine with'-i(이 )-' 2.The final phoneme of root is a consonant ; the passive sulffixes of various form are combined, and the combined phonological condition is also various. /ㄱ1/,/ㅌ(ㄾ)/,/ㅍ/,/ㅎ/ :'-i (이)-' /ㄱ(ㄺ)/,/ㄷ/,/ㅂ(ㄼ)/,/ㅈ(ㄵ)/ :'-hi(히)-' /ㄹ(ㅀ)/ or ㄷ,르-irregular declinable word :'-li(리)-' /ㄴ(ㄶ)/,/ㅁ(ㄻ)/,/ㅅ/,/ㅊ/ :'-ki(기)-'

소아환자의 깊은 진정요법 하에서 근단변위 판막술을 이용한 거대협소대의 치료

김종빈,윤형배 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

The mandibular buccal frenum is defined as a fold of mucous membrane at the posterior labial vestibule and attaches the lips and the cheeks to the alveolar mucosa, gingiva, and underlying periosteum. The buccal frenum becomes a problem when its attachment is too colse to the marginal gingiva. It may then pull on healthy gingiva, encourage plaque formation and interfere with tooth brushing. Especially, heavy buccal frenum mucogingivally results in insufficent attached gingiva, inadequate vestibular depth and high frenum attachment and also difficulty in eruption of mandibular premolar. Frenotomy, frenectomy and mucogingival surgery are used in treating heavy buccal frenum. Frenotomy with autogenous free gingival graft has been used popularly because of its stable result. But, it is difficult in younger children because of inadequate donor site, difficulty in making recipient site and behavior management. Frenotomy with apically positioned flap is considered as more efficient way for a very young child with heavy buccal frenum. Additionally, modified deep sedation with N₂O-O₂can be used as an adjunct for the effective treatment outcome. Decrease in muscle pull, adequate width of attached gingiva and increased vestibular depth can be expected from this treatment approach.