Detection of Individuals Restoring Fertility by DNA Fragment Converted into STS (sequence tagged site) in Red pepper

Lee,Jang-Soo,Lee,Keun-Hyang,Kim,Yeo-Song,Kang,Kwon-Kyoo,Nou,Ill-Sup,Hirata,Yutaka 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Co-segregation of male fertility with DNA markers selected by RAPD analysis as being potentially linked to the restorer gene (Rf) for Cytoplasmic male sterility (CMS) was analyzed using segregating F2 population. One RAPD marker directly linked to the Rf locus was identified. Amplification of OPT-02/570 using the STS primers generated a monomorphic band of each fertile plants randomly selected F2 progenies. From these results, this specific marker would be strongly linked to be restoring gene. The use of STS marker is effective in overcoming the reliability of the RAPD phenotype and improving their utility for MAS, co-dominant STS markers are especially very useful.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

지체장애인의 의료시설 접근을 위한 편의시설 개선방안에 관한 연구 : 종합병원의 외래진료부를 중심으로 Focused on the OPD in general hospital

이규일,김인순,강병근 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(계획계)

The physically handicapped people experienced many difficulties in using the medical facilities, many hospitals are not providing adequate facilities to the handicapped. This situation has been so neglected that even people with minor injuries are having trouble let alone the handicapped. A present survey was taken limited to the OPD facilities of twenty-two general and university hospitals in the metropolitan area. This study. The result of the investigation suggests that there needs to be an in-dept look into the facility manuals of OPD's for the handicapped because as of right now the Facility Installation Law is inefficient to the needs of the physically handicapped patients.

Hybrid Breeding of Red Pepper (Capsicum annum L.) Using Cytoplasmic Male Sterile Lines Combined with Phytophythora Resistance in Korea

Lee,Jang-Soo,Kang,Kwon-Kyoo,Hirata,Yutaka,Nou,Ill-Sup,Thanh,Vo-Cong 한국자원식물학회 2004 Plant Resources Vol.7 No.2

We characterized the resistances such as to waterlogging, Phytophythora and viruses in hybrids between Italian and Korean mother lines and screened them for complex resistances and agronomic traits to select elite multi-resistant lines for hybrid breeding. Resistance to waterlogging was selectable due to diversity of the resistance. Phytophythora resistance introduced from Italian lines could also be combined with resistance to other diseases and restoration abilities from cytoplasmic male sterility that has been maintained in Korean varieties.

남자 중.고등학교 역도선수들의 경쟁 상태불안이 운동수행에 미치는 영향

윤규태,이강보 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The purpose of this study is to analyse the effect of anxiety on middle and high school male weight lifters' physical performance and prove the difference of competitive state anxiety according to the demographic character of weight lifters. To accomplish the purpose of this study, a questionnaire was used which is based on 'The Competitive State Anxiety Inventory-Ⅱ:(CSA Ⅰ-Ⅱ) designed by Martens et al.(1990) after proving the reliability and validity. T-verification, one-way analysis variance, and multiple regression analysis were used in analysing the data and the average was examined using a proving method LSD. The following is the conclusion of this study through the research method and procedure The competitive state anxiety has the strongest effect on the weight lifters under the level of β=.478, P<.000 and when the level of the state self-confidence factor is β=.274, P<.005 and the cognitive state anxiety factor is β=.102, p<.302, it has a significant effect. As a result, we can conclude that the competitive state anxiety has effect on the physical performance.

Isolation and characterization of BrMDR1 a novel MDR-type ATP-binding cassette (ABC) transporter in Brassica rapa L.

Sun-Yong Lee1,Yu-Jin Jung,Kwon-Kyoo Kang 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.3

A cDNA clone encoding a MDR-like ABC transporter protein was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (named as Brmdr 1; GenBank accession no.: DQ296184 ) had a total length of 4222 bp with an open reading frame of 3900 bp, and encoded a predicted polypeptide of 1300 amino acids with a molecular weight of 143.1 kDa. The BrMDR1 protein shared 71.0, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAN28720), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Brmdr1 was a low-copy gene. Expression pattern analysis revealed that Brmdr1 constitutively expressed in the root, stem petals and stamens, but with lower expression in leaves and open flowers. The domains analysis showed that BrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in TMD1-NBD1-TMD2-NBD2 direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, Walker A , Walker B and C motif, were found. These results indicate that BrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.

모 제약회사 근로자들의 직업성 천식 유병률과 약제 분말 노출수준

오성수,최용휴,김은아,이영목,장재길,유장진,박승현,김태균,정수영,김규상,강성규 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 제약회사에서 직업상 천식의 유병 규모를 파악하고 작업공정에 대한 작업환경 측정을 통해 제약회사의 약제 분말에 대한 노출수준을 평가하고자 하였다. 방법: 제약회사에서 약제 분말에 노출되는 근로자를 대상으로 천식을 선별하기 위하여 설문조사 및 진찰, 혈액검사, 폐기능 검사 그리고 피부단자시험을 실시하였고, 각 부서별 공정에 따라서 총분진과 항생제 또는 효소제 분말에 대한 작업환경 측정을 실시하였다. 직업상 천식이 의심되는 근로자에 대하여 기관지과민성에 대한 검사(메타콜린을 이용한 비특이 기관지유발시험)와 약제 분말 기관지유발시험을 실시하여 확진하였다. 결과: 설문조사 및 진찰에서 작업 관련 천식 증상이 있는 근로자는 11명(34.4%)이었고, 약제 분말을 이용한 피부단자시험에서 1+ 이상 반응을 보인 근로자 10명(31.2%)이었다. 비특이 기관지유발시험 결과 8명(25.0%)이 양성(PC20 ≤16 mg/ml)이었다. 약제 분말 기관지유발시험을 통해 직업상 천식으로 확진된 근로자는 5명(15.6%)이었다. 작업환경 측정에서 원료를 충진하거나 투입하는 작업 그리고 정립작업에서 약제 분말에 대한 노출이 많았다. 결론: 제약회사에서 직업상 천식은 주로 제조공정 중 약제 분말에 노출된 근로자에서 발생하여, 증상 및 직업성 천식의 유병률이 높았다. 따라서 원료를 충진 또는 투입하는 작업과 정립작업 등 제조과적에서 약제 분말 노출을 줄이기 위한 세심한 주의가 필요하여, 약제 분말을 취급하는 근로자에 대해서는 천식 증상 조사뿐 아니라 정기적으로 피부단자시험, 비특이 기관지유발시험 등을 실시하여야 할 것이다. Objectives: After the investigation of one worker with occupational asthma, we surveyed the preva- lence of occupational asthma and the exposure level of pharmaceutical dust of 32 workers in a pharmaceutical company. Methods: Thirty-two of the 90 employees participated in the survey which consisted of questionnaire, blood sampling, spirometry and skin prick tests with 8 common allergens as well as 9 antibiotics and 2 enzymes. Various indices of the working environment were also measured. Subjects who had a symptom suggestive of work-related asthma or positive skin prick test were further investigated by PC20 methacholine. Nine subjects who had a PC20 result of 16 mg/ml or less (n=7) or had work-related symptoms and positive skin prick test (n=2) were referred to undergo a specific bronchial provocation test to phar- maceutical dust in an academic allergic disease center. Results: Eleven of 32 workers (34.4%) had a work-related symptom suggestive of occupational asthma. Ten (31.2%) showed positive skin prick test. 8 (25.0%) had a PC20 result of 16 mg/ml or less (indicative of significant bronchial hyperresponsiveness), and 5 (15.6%) had a positive result on the specific bronchial provocation test. Exposure levels of stuffing, input of raw materials and screening process were relatively high. Conclusions: This survey showed that pharmaceutical workers have an increased risk of occupational asthma. Although pharmaceutical factories maintain a relatively good working environment, careful control of respiratory tract exposures, especially during stuffing, input or raw materials and screening process, is important to prevent occupational asthma. Pharmaceutical workers need to undergo regular skin prick and methacholine bronchial provocation tests, as well as asthmatic symptom survey, to ensure the early detection and prevention of occupational asthma.

The Improvement of Learning and Memory Ability of Normal Persons by BF-7

Lee, Sang-Hyung,Kim, Yong-Sik,Kang, Yong-Koo,Kwon, Oh-Sang,Shin, Yong-Kyoo,Song, Jln-Ho,Lee, Moo-Yeol,Lee, Kwang-Gill,Yeo, Joo-Hong,Lee, Won-Bok,Lee, Tae-Jin,Kim, Sung-Su The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.6

To investigate whether BF-7, extracted from Bombyx mori, improved learning and memory of ordinary people, Rey-Kim Memory Test was performed with placebo group (32 persons), 200 mg BF-7 treated group (33 persons) and 400 mg BF-7 treated group (34 persons). BF-7 enhanced significantly learning and memory function in a dose-dependent manner. To know how BF-T plays such a positive role, we measured the concentration of acetylcholine in the brain from memory impairment animal model. Treatment of BF-7 significantly increased the concentration of acetylcholine. So, it is supposed that the protection of cholinergic neuron and/or keeping proper concentration of acetylcholine might be one of the mechanisms by which BF-7 improve brain function. So, our results suggest that the BF-7 is effective material for improving learning and memory ability.

Sensitization of Vanilloid Receptor Involves an Increase in the Phosphorylated Form of the Channel

Lee Soon-Youl,Lee Jae-Hag,Kang Kwon Kyoo,Hwang Sue-Yun,Choi Kang Duk,Oh Uhtaek The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.4

A vanilloid receptor (VR1, now known as TRPV1) is an ion channel activated by vanilloids, including capsaicin (CAP) and resiniferatoxin (RTX), which are pungent ingredients of plants. Putative endogenous activators (anandamide and metabolites of arachidonic acid) are weak activators of VR1 compared to capsaicin and RTX, and the concentrations of the physiological condition of those activators are not sufficient to induce significant activation of VR1. One way to overcome the weak activation of endogenous activators would be the sensitization of VR1, with the phosphorylation of the channel being one possibility. The phosphorylation of VR1 by several kinases has been reported, mostly by indirect evidence. Here, using an in vivo phosphorylation method, the VR1 channel was shown to be sensitized by phosphorylation of the channel itself by multiple pathways involving PKA, PKC and acid. Also, in sensitizing VR1, BK appeared to show activation of PKC for the sensitization of VR1 by phosphorylation of the channel.

Cross direction register modeling and control in a multi-layer gravure printing

Kang, Hyun-Kyoo,Lee, Chang-Woo,Lee, Jae-Min,Shin, Kee-Hyun 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.1

For the adaptation of the roll-to-roll printing method to printed electronics, it is mandatory to increase the resolution of the register control. Therefore, it is desired to derive a mathematical model for the register and to develop a controller to reduce register error. The mathematical model of cross direction register was derived considering both a lateral motion of a moving web and a transverse position of a printing roll. The proposed mathematical model could be used to improve the performance of the cross direction (CD) register controller in a large area, roll-to-roll printing machine. The mathematical model was validated by numerical simulations and experimental verifications in various operating conditions using a multi-layer direct gravure printing machine. The results showed that the proposed model was effective in predicting the CD register in multi-layer printing.