Sialic Acid 의 감소가 탈지분유의 단백 용해도에 미치는 영향

송재철,박현정,이재영 成均館大學校 科學技術硏究所 1987 論文集 Vol.38 No.2

The milk protein portion contains sialic acid which may be used as an index for k-casein, In comparison with non fat dry milk(NFDM) and calcium caseinate, the solubility of NFDMprotein isolates at the various pH was inferior. Another consideration for the low solubility relates to the possibility that k-casein may have been extracted or possibly modified by the action of alcohol. The k-casein is the principal stabilizing fraction for casein micelle and any changes which result in its destruction or removal would be expected to have potent results. During solvent treatment, 16.5% of sialic acid was extracted in the first extraction solvent, whereas the second treatment caused only a 4.0% loss, based on sialic acid analysis. A study was conducted on the effect of concentration of methanol on loss of sialic acid. The loss of sialic acid decreased as the concentration of methanol increased to above 62% in bath the first and second extracted solvent. it appears that loss of sialic acid is attributed to the water fraction rather than the methanol fraction. The effect of addition of the dried extracted solids on solubility was investigated. Protein solubility of NFDM protein isolates was sharply increased by 10% addition of the extracted solids and, thereafter, decreased. Efforts to restore solubility by feeding-back the extracted material supported the concept that removal of surface k-casein may have been a possible factor.

절간 고구마를 이용한 RNA 생산에 관한 연구

송재철,이종임,양한철 울산대학교 1985 연구논문집 Vol.16 No.2

RNA생산을 위한 최적 당화조건을 이용하여 생산된절간 고구당화액을 탄소원으로 효모 RNA생산능력이 가장 양호한 Cryptococcus laurentii 균주를 분리하였다. 최적 배양조건을 검토한 결과 질소원으로 ammonium sulfate 0.6%, MnSO₄0.1% 그리고 ca-pantoth-enate 400㎍/l를 무기염류, 비타민류로 각각 첨가하였을 때 최대 RNA 생산능력을 나타내었다. 기본배지의 배양조건을 검토하여 얻은 최종배지에서의 효모 균체당 RNA 생산은 30.2% 증가하였다. During an extensive screening tests of yeast on their RNA formation it was found that Cryptococcus laurentii had especially high RNA content and high dry cell weight (D. C. W) in the case of using hydrolyzate of sliced and dried sweet potatoes as a carbon source. Under the above conditions, the RNA content and yield of dry cells were investigated changing the media composition. Ammonium sulfate 0.6% was appeared to be favorable as a nitrogen sources. The optional concentration of Mn?² was showed to be 0.1%. Ca-pantothenate, 400㎍/m1, showed relatively favorable effects as a growth factor. The maximum RNA content of dry cell weight was 16.8% when grown on the medium containing above supplements under the optimal conditions.

Helicobacter pylori의 생육과 각종 유기산의 식품 보존료로서의 효과

송재철,조은경,박현정,신완철,최석영 울산대학교 2002 생활과학논문집 Vol.3 No.2

Helicobacter pylori의 생육과 각종 유기산의 식품보존료로서의 효과를 검토하였다. 우선 보존료로 현재 식품첨가물로 사용되고 있는 소르빈산(sorbic acid), 프로피온산(propionic), 안식향산(benzoic acid) 등의 H. pylori에 대한 항균능력을 검토한 결과 소르빈산, 프로피온산, 안식향산의 H. pylori에 대한 항균효과가 관찰되었으며 안식향산은 소르빈산이나 프로피온산과 비교했을 때는 별로 큰 효과를 나타내지 못하였다. 보존료의 조합에 대한 H. pylori의 생육억제효과는 0.5% 소르빈산과 0.5% 프로피온산을 조합하는 경우 나타났으며 다른 조합군과 비교할 때 큰 차이를 나타내지 못하였다. 유기산과 H. pylori의 생육관계에서는 단독 첨가시에는 항균력이 크게 나타나지 않고 조합하는 경우에만 좋은 항균력을 나타내었다. 유기산, 보존료와 H. pylori 생육관계는 유기산류는 어떤 보존료와 혼합하여도 항균력에 상승효과가 있는 것으로 나타났다. 특히 구연산의 경우 소르빈산과 조합한 경우에는 H. pylori생육이 많이 억제되었으며 그 다음이 구연산+안식향산, 구연산+프로피온산 순으로 균에 대한 항균력이 감소되었다. 또 사과산과 숙실산은 구연산과 비슷한 경향을 나타내었다. The purpose of this study was to examine the effect of various organic acids as food preservatives on the growth of Helicobacter pylori(H.pylor). The antimicrobial effects of sorbic acid, propionic acid and benzoic acid currently used as food preservatives were examined in light of the growth of Helicobacter pylori. Each of them was observed to have positive antimicrobial effect on Helicobacter pylori. However, benzoic acid wasn't higher than sorbic acid and propionic acid in antimicrobial effect. As for the combination of preservatives, antimicrobial activity of H. pylori was shown in the combination of sorbic acid(0.5%) and propionic acid(0.5%). However, its effect was not significantly different in comparison with other combinations. In the relationship of organic acids and H. pylori's growth, organic acid had no effect on antimicrobial activity when it was used alone, whereas antimicrobial effect was shown to be better in case of combination. If intermix of organic acids and preservatives and addition to the medium for suppression of H. pylori growth were achieved, organic acid exhibited synergistic effect on suppression of H. pylori's growth, even in combination of any other preservatives. In particular, in combination of citric acid and sorbic acid, the growth of H. pylori was shown to be remarkable suppression. And the effectiveness on suppression of H. pylori's growth was lowered in order of citric acid + bezoic acid and citric acid + propionic. acid. It was also found that the antimicrobial effects of malic acid and succinic acid were similar to those of citric acid.

Modified FR-4의 임상적용례

송재혁,이긍호,최영철 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.2

전치부 개교합은 상·하악 전치부가 폐구 시 절단 기능에 필수적인 수직피개가 결여되어 있는 상태를 말한다. 일반적인 원인으로는 손가락 빨기, 혀 내밀기, 유아성 연하, 비호흡 부전, 골격성장의 이상 또는 이들이 복합적으로 작용되어 나타날 수 있으며, 어떤 종류의 부정교합과도 함께 나타날 수 있다. 전치부 개교합을 위한 치료 방법은 다양하지만, Rolf Fra ***** nce에 의해 고안된 Fra ***** nkel appliance(FR-4)는 골격성 I급 혹은 II급 부정교합과 함께 나타난 개교합의 치료에 특히 효과적인 것으로 알려져 있다. 서양인에서는 골격성 II급 부정교합이 높은 빈도로 나타나고, 개교합도 이와 함께 나타나는 경우가 많다. 그러나 동양인에서는 골격성 III급 부정교합의 빈도가 높고, 개교합 또한 이와 같은 골격형태와 함께 나타나는 경우가 gms히 있다. 이러한 문제점을 갖는 환자에 있어서 전통적인 FR-4의 사용이 개교합의 교정에 도움이 되었을지라도, 골격성 III급 부정교합을 악화시키는 것으로 나타났다. 두 가지 문제점을 동시에 교정하기 위하여, labial bow를 하악 전치부에, labial pads를 상악 전치부에 위치시킨 modified FR-4를 고안하게 되었다. 다음의 임상례는 전치부 개교합을 동반한 골격성 III급 부정교합을 보이는 환자를 대상으로, 전통적인 FR-4와 modified FR-4를 이용하여 치료한 결과를 비교한 것이다. 첫 번째 임상례는 전통적인 FR-4를 사용한 경우로서 전치부 개교합은 개선되었으나 골격성 III급 부정교합의 형태가 심해졌다. 그러나 두 번째와 세 번째 임상례에서는 modified FR-4를 사용한 결과, 전치부 개교합과 III급 부정교합이 동시에 개선된 것으로 나타났다. Anterior open bite is one in which the teeth in the anterior portion of the maxilla and mandible are vertically apart and lack the overlapping necessary for the incisive function when the mandible is in closed position. Anterior open bite is a result of the interaction of many different etiologic factors including thumb and finger sucking, lip and tongue habits, airway obstruction, skeletal growth abnormalities and its tendency may appear with any type of skeletal patterns, such as Class I, II or III malocclusion types. Though the treatment methods for anterior open bite are various, the conventional FR-r, designed by Rolf Fr ***** nkel, is known to be effective in treating open bite cases with Class I or II skeletal patterns. It is �� 새 that an incidence of skeletal Class II is high in the Occidentals, and open bite is accompanied by these malocclusion type in many cases. However, an incidence of skeletal Class III is high in the Orientals, and open bite is sometimes accompanied by skeletal Class III in many cases. Although the use of the conventional FR-4 was effective in the treatment of open bite, skeletal Class III would be worsened. So, a modified FR-4(placing the labial bow in the lower, the labial pads in the upper) was designed for the treatment of patients showing skeletal Class III and open bite.

낭성법랑모세포종, 함치성낭, 치성각화낭의 방사선소견과 Ki-67, PCNA, Cytokeratin 발현과의 연관성에 관한 연구

송만용,이삼선,이진구,이원진,허민석,이재일,민병무,최순철 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To compare the proliferation potential of the epithelial cells between unicystic ameloblastoma (UA), dentigerous cyst (DC), and odontogenic keratocyst (OKC) and to correlate this proliferation potential with the radiographic features of these three pathoses. Materials and Methods : Immunohistochemical expression of PCNA, Ki-67, and cytokeratin as a proliferation marker were assessed for 15 cases of UA, 15 cases of DC, and 15 cases of OKC. The degree of immunochemical expression of three proliferation markers were correlated with the radiographic features, especially cortical expansion (negative and positive) and shape of border (scalloped and round). Results : Using PCNA and Ki-67, OKC showed the highest proliferation potential and UA the lowest. Statistically significant differences were found between the OKC and the UA (p.0.05). However, no statistically significant difference was present according to the radiographic features in all pathoses. Using cytokeratin, there was no significant differences of proliferation potential among three pathoses. Conclusions : OKC epithelium has the most intense proliferation potential, followed by the dentigeous cyst and then unicystic ameloblastoma. There is no significant relation between the radiographic features and the proliferation potential of epithelium of these three pathoses.

열처리 시 소금첨가에 의한 풋콩의 색과 Chlorophyll 함량 변화

송재연,김철재,안길환 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.2

풋콩의 열처리 시 소금의 영향을 알아보기 위해 이전의 실험에서 비슷한 수치를 나타낸 80℃-30min. 90℃-20min, 100℃-10min의 조건에서 color와 chlorophyll 함량을 측정하였다. 녹색과 깊은 관련이 있는 -α값(colorimeter parameter)과 chloropyll α의 두 수치만을 비교해 보았을 때, -α값은 100℃-10min이 높게 나온 반면, chlorophyll 함량의 경우 소금을 첨가한 군이 비 첨가군보다 높은 값을 나타내었다. 온도와 시간이 color와 chlorophyll 함량 파괴에 미치는 영향을 알아보기 위해 반응속도상수(reaction rate constant)와 활성화 에너지(activation energy)값을 구해보았다. 그 결과 반응속도 상수는 온도가 10℃증가함에 따라 2배 가량 증가하는 것을 볼 수 있었고 이는 Arrhenius 이론과 일치하였다. 소금의 첨가는 seed의 색(greenness)만을 제외하고 전체적으로 chlorophyll과 color의 파괴속도를 저하시키는 역할을 하였다. Pod에서 chlorophyll α감소를 위한 활성화 에너지는 146.63㎉/㏖(소금군)로 비소금군의 138.02㎉/㏖ 보다 높은 값을 나타내었고 이는 소금이 색소저하 반응의 속도를 낮춤을 의미하였다. 결과적으로 풋콩의 열처리 시 소금의 첨가는 단순히 맛의 변화뿐만 아니라 chloropyll 잔량을 높여 풋콩의 색소를 안정화시키는 역할을 하였다. Vegetable soybeans were blanched at 80, 90 and 100℃ for 30, 20 and 10min, respectively. NaCl (3%) was also used to measure the protective effect of soybean color. The color of vegetable soybeans was measured by colorimeter, -a value (greenness) was highest at 100℃-10min. However, the chlorophyll contents was highest at 80℃-30min. NaCl (3%) decreased the loss of chlorophyll in blanched vegetable soybeans. The reaction rate constant for the thermal degradation of chlorophyll and greenness doubled per 10℃. The activation energy chlorophyll a of pod for thermal degradation of chlorophyll a in pods were 138.02 (unsalted), 146.63 (salted) ㎉/㏖, respectively.

뮤코다당증(Mucopolysaccharidosis)환아의 치은 증식

송주현,장철호,김영재,한세현,이상훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

뮤코다당증(Mucopolysaccharidosis, MPS)은 gIycosaminoglycans (GAGs)의 분해에 필요한 라이소좀 효소(lysomal enzymes)의 결함으로 GAGs의 대사 산물이 세포의 라이소좀내에 축적되어 점차 세포와 조직, 기관의 기능 이상을 초래하고 신체 질환과 정신 발달 지연을 가져오는 질환이다. 본 증례는 심각한 치은 증식을 가진 뮤코다낭증 환아를 전신 마취 하에 치은 절제술을 시행한 치험례이다. 소아치과 의사는 뮤코다당증 환아의 구강내 증상과 치과 치료시 주의 사항에 대해 숙지해야 하고, 내과의와 연계하여 환아의 전신 상태 변화에 민감하게 대처하여 치과 치료를 시행해야 한다. Mucopolysaccharidosis (MPS) is a disorder of storage in which there is excessive accumulation of glycosaminoglycans(GAGs) from lysosomal enzyme defect. Lysosomal accumulation of GAGs eventually results in cell, tissue and organ dysfunction. This patient may manifest mental retardation and physical disorders. This clinical report presents a girl with MPS having severe gingival hyperplasia. Gingivectomy was performed under general anesthesia. The pediatric dentist must be aware of oral manifestations present in the MPS. The approach to dental management will require teamwork between the dentist and the patient' s physician.

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

여성생식계 상피세포에서 Glutathione S-Transferase 출현도에 관한 면역조직화학적 관찰

송계용,김석중,김미경,박용욱,유재형,박상철 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.2

Both placental form (GST-Pi) and basic form (GST-L) of glutathione S-transferase were studied in the epithelium of normal, tumor and tumor like lesions of female genital system. The results obtained were as follows : 1. Staining reaction of GST-Pi were moderate in normal cytotrophoblasts, mild in syncytiotrophoblasts, strong in corpus luteum, moderate to strong in salpingeal mucosa and in proliferative glands, moderate in secretory glands of indometrium and moderate in superficial squamous epithelium of cervix 2. Staining reaction of GST-L were mild in cytotrophoblasts and in syncytiotrophoblasts, of chorionic villi in corpus luteum, negative in salpingeal mucosa, very weak in proliferative glands, mild in secretory, glands of endometrium and in superficial squamous epithelium of cervix. 3. Staining reaction of GST-L were mild in cytotrophoblast and syncytiotrophoblast of choriocarcinoma, moderate in cytotrophoblasts, mild in syncytiotrophoblasts of hydatidiform mole, negative in mucinous epithelium of cust adenoma, in hyperplastia and adenocarcinoma, and mild in adenocarcinoma of cervix. 4. Staining reaction of GST-L were very weak in cytotrophoblasts and syncytiotrophoblasts of choriocarcinoma and in hydatidiform mole, negative in mucinous epithelium of cust adenoma, very weak in serous eptiethlium, negative to mild in hyperplastic and adenocarcinoma, mild in squamous cell carcinoma and adenocarcinoma of cervix. Therefore, staining reaction and distribution of GST-Pi and L isoengumes were different in normal and neoplastic cell of trophoblasts, salpinx, endometrin and cervix

국내산 석량종 풋콩의 형태학적인 특성과 성분분석

송재연,안길환,김철재 선문대학교 자연과학대학 2000 자연과학논총 Vol.3 No.-

풋콩종자 중 석량품종의 형태학적인 특성과 일반성분을 분석하여 품질을 측정하기 위하여 본 과제를 수행하였다. 풋콩의 생협장은 3립이 6.25cm, 2립이 5.76cm, 1립이 4.75cm로서 고품질 기준인 4.5cm를 넘었으나 생협폭은 3립만이 1.32cm로 1.3cm인 기준을 통과하였다. 생협색은 진녹색 (-a 값, 11.84) 이었고, 모용색 은 회백색 (줄기) 또는 연갈색 (생협)이었다. 2-3립협율은 72.82% 이었다. 풋콩의 일반성분은 수분, 65.5%; 단백질, 15.3%; 탄수화물, 12.7%; 지질, 4.5%; 회분, 2.0% 이었 다. 회분은 Ca, 59.7mg/l00g 풋콩 Fe, 4.0mg/100g; p, 0.4mg/100g; Na, 1.9mg/100g; K, 571.8mg/100g; Mg, 102.7mg/100g 이었다. In this report, morphological character and proximate composition of Seokryang variety of Glycine max (L.) Merrill were evaluated. Length of 3-seed-pod, 2-seed-pod, and I -seed-pod were 6.25, 5.76, and 4.75cm, respectively, which passed the length criterion of high quality, 4.5cm. Width of 3-seed-pod was 1.32cm, whereas others were less than 1.3cm, the width criterion of high Quality. The color of pod, pubescence on pod, and pubescence on stem was fresh-green (-a value, 11.84), light-brown, and gray, respectively. The ratio of 2- or 3-seeds-pod to the total pod was 72.8296. The composition of seeds was moisture, 65.5%; protein, 15.3%; carbohydrate, 12.7%; lipid, 4.5%; ash, 2.0%. The components and contents of ash were Ca, 59.7mg/100g seeds; Fe, 4.0mg/100g; P, 0.4mg/100g; Na, 1.9mg/100g; K, 571.8mg/100g; Mg, 102.7mg/100g.