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Progress in the development of heating systems towards long pulse operation for KSTAR
Kwak, J.G.,Bae, Y.D.,Chang, D.H.,Chang, D.S.,Hong, B.G.,Hwang, C.K.,In, S.R.,Jeong, S.H.,Jin, J.T.,Jung, K.S.,Kim, B.R.,Kim, J.,Kim, S.K.,Kim, T.S.,Lee, D.W.,Lee, K.W.,Oh, B.H.,Seo, C.S.,Seo, M.S.,Yoo International Atomic Energy Agency 2007 Nuclear fusion Vol.47 No.5
<P>Construction of the Korea superconducting tokamak advanced research (KSTAR) tokamak is in its final phase. For the long-pulse KSTAR discharges, the ion cyclotron range of frequencies (ICRF) and neutral beam injection (NBI) heating systems are expected to play important roles through a selective heating of ions and electrons, control of the plasma pressure and current profiles, a core fuelling and beam diagnostics for the KSTAR. In addition, the ICRF system is expected to be used for possible discharge cleaning and assisting in the tokamak startup. In this paper, the recent progress in the development of the ICRF and the NBI heating systems is described. The four-strap ICRF antenna has been successfully tested for a voltage up to 41 kV for a pulse length of 300 s (to 46 kV for 20 s) in a test chamber. A prototype KSTAR NBI system has been developed. At present, the system has successfully produced a 1 MW beam power for 200 s and a 3.5 MW output beam power for 4 s.</P>
간호 대학생의 성 지식, 성 태도와 성 건강 간호 장애감 간의 관계
김다연,김은아,노수경,류아인,박나은,박지혜,서정민,심효정,주성민,한진실,이건정,박수민 이화여자대학교 간호과학대학 2017 이화간호학회지 Vol.- No.51
Purpose: The purpose of this study was to investigate correlation among sexual knowledge, attitude and Barriers to patient’s sexual health of nursing undergraduate students and to provide objective data to promote sexual health-care. Method: Using a descriptive correlation, 304 nursing undergraduate students were recruited through convenience random sampling from August 1st to December 20th, 2016. Data were analyzed using average, standard deviation, percentage, t-test, ANOVA, Scheffe test, Pearson’s correlation and Multiple linear regression analysis using the SPSS 21.0 program. Results: The correlation between Sexual knowledge and Sexual attitude is statistically significant(r=.289, p<.01). As the level of sexual knowledge is higher, the sexual attitude is more open. And, Sexual knowledge(r=-.307,p<.01) and Sexual attitude(r=.180,p=.002) had a negative relationship with Barriers to patient’s sexual health. Also, Sexual knowledge was the greatest influencing factor on Barriers to patient’s sexual health. Conclusion: This study showed as the level of Sexual knowledge is higher, the Sexual attitude is more open. As the degree of Sexual knowledge is lower, the Sexual attitude is more conservative, and Barriers to patient’s sexual health is more higher. Also, Sexual knowledge is the most influential factor on Barriers to patient’s sexual health.
Seo, S H,Kim, K S,Park, S H,Suh, Y S,Kim, S J,Jeun, S-S,Sung, Y C Nature Publishing Group 2011 Gene Therapy Vol.18 No.5
<P>Owing to its tumor tropism and prolonged transgene expression, mesenchymal stem cell (MSC) has been considered as an ideal delivery vehicle for cancer gene therapies or therapeutic vaccines. In this study, we demonstrated that intratumoral (i.t.) injection of MSCs expressing modified interleukin-12 (MSCs/IL-12M) exhibited stronger tumor-specific T-cell responses and antitumor effects as well as more sustained expressions of IL-12 and interferon (IFN)-γ in both sera and tumor sites than did IL-12M-expressing adenovirus (rAd/IL-12M) in mice bearing both solid and metastatic tumors. Subcutaneous (s.c.) injection of MSCs/IL-12M at contralateral site of tumor exhibited similar levels of serum IL-12 and IFN-γ as i.t. injection, but much weaker antitumor effects in both B16F10 melanoma and TC-1 cervical cancer models than i.t. injection. Although intravenous (i.v.) injection elicited earlier peak serum levels of cytokines, it induced weaker tumor-specific T-cell responses and antitumor effects than i.t. injection, indicating that serum cytokine levels are not surrogate indicators of antitumor effects. Taken together, these results indicated that MSC is more efficient than adenovirus as a cytokine gene delivery vehicle and that i.t. injection of MSCs/IL-12M is the best approach to induce strong tumor-specific T-cell responses that correlate with anti-metastatic effects as well as inhibition of solid tumor growth, although MSCs themselves have an ability to migrate into the tumor site. In addition, MSCs/IL-12M embedded in Matrigel (MSCs/IL-12M/Matrigel) exhibited significant antitumor effects even in immunodeficient mice such as SCID and BNX mice lacking T, B and natural killer (NK) cells, but not in IFN-γ knockout mice. Our findings provide an optimal approach for designing an efficient clinical protocol of MSC-based cytokine gene therapy to induce strong tumor-specific T-cell responses and therapeutic anticancer efficacy.</P>
Membrebe, J.D.,Yoon, N.K.,Hong, M.,Lee, J.,Lee, H.,Park, K.,Seo, S.h.,Yoon, I.,Yoo, S.,Kim, Y.C.,Ahn, J. Elsevier 2016 Veterinary immunology and immunopathology Vol.170 No.-
<P>Enolase (ENO) is one of the surface-exposed proteins of Streptococcus iniae, which previously had been identified as a plasminogen-binding protein. In this study, ENO was evaluated to induce cross-protective immunity against S. iniae and Streptococcus parauberis which are major pathogens causing streptococcosis in fish. Immunoblot analysis shows that S. iniae recombinant ENO (rENO) produced in Escherichia coli was cross-reactive with antisera against S. iniae, and S. parauberis serotype land II. In the challenge experiment of streptococcal infection after vaccination in zebrafish, rENO elicited a similar protection with a whole cell bacterin against S. iniae and S. parauberis, which suggests its feasibility as an efficient vaccine against streptococcosis. (C) 2016 Elsevier B.V. All rights reserved.</P>
소선이,마지은,고지윤,김은혜,박현화,서유진,서형은,유은미,이하늬,이혜인,정지연,현혜리,이자형,정덕유,강지숙 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43
Purposes :The purposes of this study was to investigate the self-managing status according to the life styles and the diabetic knowledge of early adulthood. The subjects in this study were 240people, the early adult aged twenties to thirties living in the metropolitan area. Data were collected by using self-administered questionnaires that measure about the knowledge of diabetes; the dietetic behavior; the knowledge of diet; the exercise behavior; the knowledge of exercise. Descriptive statistics, t-test, ANOVA, and correlation were used for data analysis. Result :1. At the knowledge of diabetes degree based on the general character, the subjects who had experiences of diabetic education have higher degree of the diabetic knowledge. 2. At the knowledge of diabetes degree according to the life style, the subjects who have regular diet are appeared highly. Also the subjects who don't take much food of animal origin have higher degree. 3. At the knowledge of diet based on the life style, the subjects who don't keep three times meals a day are appeared highly. Also the subjects who have regular diet have higher degree. 4. At the knowledge of exercise according to the life style, the subjects who take extra nutritions 3-4 times a week are appeared highly. Although the subjects tend to know about dietary, exercise and health management, it showed that they actually don't perform what they know in their everyday of life. The reason for this could be the lack of perceived seriousness about diabetes mellitus. Thus as nursing professionals, we should provide them proper health education and support. Moreover, advanced research on the empowerment is warranted.
Rosiglitazone-mediated dendritic cells ameliorate collagen-induced arthritis in mice
Byun, S.H.,Lee, J.H.,Jung, N.C.,Choi, H.J.,Song, J.Y.,Seo, H.G.,Choi, J.,Jung, S.Y.,Kang, S.,Choi, Y.S.,Chung, J.H.,Lim, D.S. Pergamon Press 2016 Biochemical pharmacology Vol.115 No.-
<P>Rosiglitazone is a selective ligand for peroxisome proliferator-activated receptor-gamma (PPAR-gamma), which serves diverse biological functions. A number of autoimmune disease models have been used to examine the anti-inflammatory and immunosuppressive effects of tolerogenic dendritic cells (tDCs). The aim of the present study was to investigate whether rosiglitazone-mediated DC (Rosi-DC) therapy suppressed arthritis in a collagen-induced arthritis (CIA) mouse model. Rosi-DCs were generated by treating immature DCs with TNF-alpha, type II collagen, and rosiglitazone. CIA mice then received subcutaneously (s.c.) two injections of Rosi-DCs. The severity of arthritis was then assessed histopathologically. The phenotypes of the DC and regulatory T (Treg) cell populations in CIA mice were determined by flow cytometry and the effect of Rosi-DCs on the secretion of autoimmunity-inducing cytokines was examined by ELISA. Rosi-DCs expressed lower levels of DC-related surface markers than mature DCs. Histopathological examination revealed that the degree of inflammation in the paws of Rosi-DC-treated mice was much lower than that in the paws of PBS-treated CIA mice. Taken together, these results clearly show that rosiglitazone-mediated DCs ameliorate CIA, most likely via the induction of antigen-specific Treg cells. (C) 2016 Elsevier Inc. All rights reserved.</P>
Moon, S.M.,Kim, J.S.,Kim, H.J.,Choi, M.S.,Park, B.R.,Kim, S.G.,Ahn, H.,Chun, H.S.,Shin, Y.K.,Kim, J.J.,Kim, D.K.,Lee, S.Y.,Seo, Y.W.,Kim, Y.H.,Kim, C.S. Society for Bioscience and Bioengineering, Japan ; 2014 Journal of bioscience and bioengineering Vol.117 No.5
A novel fibrinolytic enzyme was purified from Lyophyllum shimeji, a popular edible mushroom in Asia. The enzyme was purified using combination of anion exchange chromatography on a Mono Q 5/5 column and size exclusion gel filtration chromatography on Superdex 200 100/300 column. This purification protocol resulted 80.9-fold purification of the enzyme and a final yield of 5.7%. The molecular weight of the purified enzyme was estimated to be 21 kDa by SDS-PAGE and size exclusion gel filtration. The N-terminal amino acid sequence was found to be ITFQSASP, which is dissimilar from that of known fibrinolytic enzymes. The purified enzyme was a neutral protease with an optimal reaction pH and temperature of 8.0 and 37<SUP>o</SUP>C, respectively. Enzymatic activity was inhibited by Cu<SUP>2+</SUP> and Co<SUP>2+</SUP>. It was also significantly inhibited by PMSF and TPCK. Furthermore, it was found to exhibit a higher specificity for S-7388, a well-known chymotrypsin chromogenic substrate, indicating chymotrypsin like serine metalloprotease. The relative fibrinolytic activity of 5 μg purified enzyme have two fold more activity than 1 unit/ml of plasmin on fibrin plate. Furthermore, purified enzyme preferentially hydrolyzed the Aα-chain followed by the Bβ- and γ-chain of fibrinogen, which is precursor of fibrin. Therefore, these data suggests that the fibrinolytic enzyme derived from edible mushroom, L. shimeji, might be useful for thrombolytic therapy and preventing thrombotic disease.
PCR 기법을 이용한 한국재래산양의 α s2-casein 유전자의 특성
이상훈,상병찬,한성욱,김지애,서길웅 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4
This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from the analysis of genetic characteristics of α_(S2-) casein gene in Korean Native goat using PCR-RFLP. This study confirmed the amplified product of 1.3kb fragment obtained from the amplification of a α_(S2-)casein loci by PCR. The frequency of a α_(S2-)casein genotype in Korean Native goat was 100% for AA type and the frequencies of a α_(S2-)casein genotype in Saanen were 80 and 20% for AA and AB genotypes. The frequency of a α_(S2-)casein A allele was 1.000 in Korean Native goat, and the frequencies of a α_(S2-)casein A and B alleles were 0.900 and 0.100 in Saanen, respectively. The nucleotide sequence of a α_(S2-)casein gene of Korean Native goat was 99.0% homology with 12 nucleotide sequences difference of that of goat reported in GeneBank(AJ238965.1, AJ238967.1) and was 95.0% homology with 57 nucleotide sequences difference of that of Holstein reported in Gene Bank(M94327). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphisms and sequences for a α_(S2-)casein gene should be used as basic data for preservation and improvement of genetic resources in Korean Native goat breeding.