韓·美·日 老人住宅硏究의 방향

신경주,조재순,곽인숙,최정신 漢陽大學校 韓國生活科學硏究所 1995 韓國 生活 科學 硏究 Vol.- No.13

The purpose of this research was to trace the trends in the research subjects related to housing for the elderly in Korea, U.S.A, and Japan. One hundred sixty eight researches were selected from six research journals besides Masters' and Ph. D theses published in Korea, 164 from five research journals and three research subject-lists in Japan, and 124 from three research journals in U.S.A. There were some common research areas of the elderly housing among the three countries such as facilities required for the elderly housing, general issues of the elderly housing, etc. There was some differences in the research areas. For instance, intergenerational coresidence was mainly studied in Korea. The results showed that housing policies as well as research methods related to the elderly housing are beginning areas and must be further developed to meet the rising needs of housing for the elderly in Korea.

Time - resolved photoluminescence spectroscopy of InGaN multiple quantum wells

Joo In Lee,Eun-joo Shin,J. Y. Leem,S. T. Kim,G. S. Lim,H. G. Lee 한국진공학회(ASCT) 2000 Journal of Korean Vacuum Science & Technology Vol.4 No.1

We have fabricated by metal organic chemical vapor deposition (MOCVD) In_(0.13)Ga_(0.87)N/GaN multiple quantum well (MQW) with thickness as thin as 10 Å and barriers also of th same width on (0001) sapphire substrate. We have investigated this thin MQW by steady-state and time-resolved photoluminescence (PL) in picosecond time scale in a wide temperature range from 10 to 290 K. In the PL at 10 K, we observed a broad peak at 3.134 eV which was attributed to the quantum well emission of InGaN. The full width at half maximum (FWHM) of this peak was 129 meV at 10 K and its broadening at low temperatures was considered to be due to compositional fluctuations and interfacial disorder in the alloy. The narrow width of the quantum well was mainly responsible for the broadening of the emission linewidth. We also observed an intense and sharp peak at 3.471 eV of GaN barrier. From the temperature dependent PL measurements, the activation energy of the InGaN quantum well emision peak was estimated to be 69 meV. The lifetime of the quantum well emission was found to be 720 ㎰ at 10 K, which was explained in terms of the exciton localization arising from potential fluctuations.

법랑모세포 분화와 성숙과정에서 OD314의 발현

박주철,안성민,김흥중,정문진,박민주,신인철,손호현 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.5

법랑모세포는 법랑질을 형성하고 유지하는 세포로, 법랑질의 유기기질을 분비하고 법랑질 석회화 과정에도 관여한다. 치아 발생과정에서 법랑모세포의 분화는 순차적인 상피-간엽 상호작용에 의하여 조절되나, 분화나 성숙과정의 정확한 기전은 아직까지 잘 알려져 있지 않다. 최근에 상아모세포에서 처음 발견된 OD314가 치아 발생과정에서 상아질을 형성하는 상아모세포 뿐 아니라 법랑모세포에도 발현된다고 하였다. 이에 본 연구에서는 생쥐 하악 전치의 다양한 시기의 법랑모세포를 이용하여, 형태학적 분석과 in-situ hybridization에 의한 OD314 mRNA의 발현 그리고 OD314 항체를 이용한 면역조직화학적 분석을 통하여 OD314 유전자의 법랑 모세포 분화와 성숙과정에서의 역할을 연구하여 다음과 같은 결과를 얻었다. 1. 형태학적으로 법랑모세포는 분화 단계에 따라 분비 전단계 법랑모세포, 분비기 법랑모세포, 성숙기의 평탄끝 법랑모세포와 성숙기의 주름끝 법랑모세포로 구분되었다. 2. OD314 mRNA는 분비기의 법랑모세포에서부터 발현되기 시작하여 법랑모세포가 성숙해갈 수록 그 발현이 증가하였다. 3. OD314 단백질은 분비 전단계의 법랑모세포에서는 발현되지 않고, 분비기의 법랑모세포에서는 세포질에 전체적으로 발현되었다. 성숙기의 평탄끝 법랑모세포와 주름끝 법랑모세포에서는 세포의 근심과 원심끝단에 OD314 단백질이 강하게 발현되었다. 이상의 결과를 종합하여 OD314는 법랑모세포의 분화와 성숙과정에서 세포질 내부에서 특징적인 역할을 하는 것으로 사료된다. Ameloblasts are responsible for the formation and maintenance of enamel which is an epithelially derived protective covering for teeth. Ameloblast differentiation is controlled by sequential epithelial-mesenchymal interactions. However, little is known about the differentiation and maturation mechanisms. OD314 was firstly identifled from odontoblasts by subtraction between odontoblast/pulp cells and osteoblast/dental papilla cells, even though OD314 protein was also expressed in ameloblast during tooth formation. In this study, to better understand the biologcal function of OD314 during amelogenesis, we examined expression of the OD314 mRNA and protein in various stages of ameloblast differentiation using in-situ hybridization and immunohistochemistry. The results were as follows : 1. The ameloblast showed 4 main morphological and functional stages referred to as the presecretory, secretory, smooth-ended, and ruffle-ended. 2. ○D314 mRNA was expressed in secretory ameloblast and increased according to the maturation of the cells. 3. OD314 protein was not expressed in presecretory ameloblast but expressed in secretory ameloblast and maturative ameloblast. OD314 protein was distributed in entire cytoplasm of secretory ameloblast. However, OD314 was localized at the proxiamal and distal portion of the cytoplasm of smooth- ended and ruffle-ended ameloblast. These results suggest that ○D314 may play important roles in the ameloblast differentiation and maturation.

P255 : Relationship between the HLA-G 14bp insertion/ deletion polymorphism and susceptibility to autoimmune disease: a mata-analysis

( June Hyuck Yim ),( Tae In Kim ),( In Jung Kang ),( Ki Heon Jeong ),( Su Kang Kim ),( Joo Ho Chung ),( Min Kyung Shin ),( Mu Hyoung Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Numerous studies have investigated the potentialrelationship of human leukocyte antigen (HLA)-G 14bp insertion/deletion (INS/DEL) polymorphism with autoimmune disease (AID). However, results from published data were inconclusive. Objectives: Our aim in this study was to determine whether the 14bp INS/DEL polymorphism in the HLA-G gene contributes to the risk of AID. Methods: A systemic literature search of PubMed and Embase databases was conducted to identify eligible studies investigating the association of HLA-G 14bp INS/DEL polymorphism with AID. The final analysis included 13 publications with a total of 6462 individuals. Results: Overall, no significant association between HLA-G 14-bp INS/DEL polymorphism and overall AID was detected in all comparison models. Further subgroup analyses based on AID types and ethnicity showed no significant association. Conclusion: Our results suggest that the HLA-G 14bp INS/DEL polymorphism might not be related to the development of AID. Further studies with larger sample size are warranted to validate our finding.

본태성 고혈압에서의 혈중 Atrial Natriuretic Peptide 농도와 Renin - Aldosterone 계의 상관관계에 대한 연구

신현호(Hyun Ho Shin),박원근(Won Kun Park),한인권(In Kwon Han),김은주(Eun Joo Kim),김선우(Sun Woo Kim),송정상(Jung Sang Song),배종하(Jong Hoa Bae),최영길(Young Kil Choi) 대한내과학회 1987 대한내과학회지 Vol.32 No.6

N/A Atrial Natriuretic Peptide (ANP) has been shown to posseses a variety of important biologic activities, in- cluding natriuresis, relaxation of vascular smooth muscle, and reduction in aldosterone secretion. This raises the possibility that ANP may play a part in the regulation of volume homeostasis and may be involved in the pathogenesis of essential hypertension. We investigated the relationship of ANP and reninaldosterone system in 12 normal subjects and 24 patients with essential hypertension. The results were as follows; 1) Plasma levels of ANP in patients with essential hypertension were significantly higher than in normotensive controls (131.1±21.7pg/ml VS 102.3±17.4 pg/ml). 2) There were no significant difference in plasma renin activity or aldosterone levels between normotensive and hypertensive subjects. 3) The patients with low renin essential hypertension had a tendency of increased ANP concentration, compared with high renin hypertension patients. 4) Plasma levels of ANP were positively and significantly correlated with blood pressure (Systolic r=0.38, p<0.05; diastolic r=0.49, p<0.005). These results suggests that increased secretion of ANP in essential hypertension may be a compensatory response to a diminished capacity for sodium excretion.

서방형 Diltiazem제제의 개발을 위한 약동학/약력학적 특성 검토

유경상,배균섭,조주연,장인진,신상구 大韓臨床藥理學會 1998 臨床藥理學會誌 Vol.6 No.2

Effect of Platelet-Rich Fibrin on Repair of Defect in the Articular Disc in Rabbit Temporomandibular Joint by Platelet-Rich Fibrin

( Hyun Su Baek ),( Hye Sung Lee ),( Bok Joo Kim ),( In Kyo Chung ),( Chul Hoon Kim ),( Sun Mi Jin ),( Hie Sung Hwang ),( Sang Hun Shin2 ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.6

The objective of this study was to evaluate platelet-rich fibrin (PRF)’s effectiveness in repairing articular disc defect in the temporomandibular joint (TMJ) of rabbits. Eight rabbits were divided into four groups of two rabbits each, corresponding to groups A, B, C, and D. Both TMJs of all of the rabbits were used in the experiments: the right joints comprised the experimental groups, and the left ones, the control groups. The disc defect was circular and 2 mm in diameter. In the experimental groups, the PRF was compressed into the defect, whereas the control group defects were left untreated. A, B, C, and D groups were sacrificed at the 1st, 2nd, 4th and 6th weeks, respectively. The defects of each control group exhibited no specific changes. Contrastingly, in each experimental group, there was an increased number of chondroblasts at the margins of the defects, along with accelerated cell differentiation and a columnar cell arrangement observable at the time of cell differentiation. The experimental groups showed inflammatory cell infiltrations and fibrosis by the 1st week, maturation of chondrocytes by the 2nd week, and proliferation by the 4th week, after which the defects began to be filled with chondrocytes, a process that was complete after the 6th week. In the histological evaluation (H-E), the experimental groups showed significant increases of chondroblasts after the 2nd and 4th weeks, as well as regular columns of chondrocyte arrays observable during cell division. After 6 weeks, the defects were filled with chondrocytes.

Nuclear factor I-C가 치근발생 과정에서 Hertwig's 상피초 형성에 미치는 영향

신인철,박주철,정문진,오현주,박선화,이창섭,김흥중,Shin, In-Cheol,Park, Joo-Cheol,Jeong, Moon-Jin,Oh, Hyun-Ju,Park, Sun-Hwa,Lee, Chang-Seop,Kim, Heung-Joong 대한소아치과학회 2005 大韓小兒齒科學會誌 Vol.32 No.3

치아의 형성은 상피-간엽간의 상호작용을 통해 조절되어지는 복잡한 발생과정이다. 지금까지 치관의 발생에 관여하는 유전자 및 그들의 신호전달경로에 관한 연구는 다수 진행되어 왔지만 치근의 발생을 조절하는 기전에 대해서는 별로 알려진 것이 없다. 최근에 NFI-C knock out 생쥐에서 정상치관에 비정상적인 치근을 가지는 치아가 보고되었다. 본 연구의 목적은 NFI-C가 어떻게 치근의 형태와 상아모세포의 분화에 관여하는지를 규명하는 것이다. NFI-C knock out 생쥐의 치근 발생동안에 HERS의 역할을 연구하고자 cytokeratin 면역조직화학적방법과 치근상아질의 특성을 규명하기 위해 DSPP mRNA in-situ hybrydization법을 수행하였다. 1. NFI-C knock out 생쥐의 치근형성시 HERS의 역할 Wild type과 knock out type 모두에서 cytokeratin은 모든 HERS 세포들과 반응하였고, HERS와 법랑상피 사이의 양성반응세포들의 연속성은 치경부 부위에서 소실되었다. Knock out type에서 치근상아질이 침착된 후, cytokeratin 양성-HERS 세포들은 치경부에서 불규칙한 배열과 극성의 상실을 보였다. 2. NFI-C knock out 생쥐의 치근상아질의 특성 DSPP mRNA의 발현은 wild type에서 치관과 치근상아질의 상아모세포 모두에서 강한 발현을 보인 반면, knock out type에서는 치관부위 상아질의 상아모세포에서만 강한 발현을 보였다. 3. NFI-C knock out 생쥐의 치근 발생과정에서 HERS는 치관으로부터 정상적인 확장을 보인 반면, 치근부위에서의 상아 모세포 분화는 실패하였다. 위의 결과들로 보아 NFI-C는 치근형성 과정에서 상아모세포 분화에 중요한 역할을 하는 것으로 사료된다. Tooth formation is a complex developmental process that is mediated through a series of reciprocal epithelial-mesenchymal interactions. Several signal pathways and transcription factors have been implicated in regulating molar crown development, but relatively little is known about the regulation of root development. It was reported that NFI-C knockout mice showed abnormal root formation with normal crown. The aims of this study are to elucidate how the NFI-C regulate the determine of root shape and odontoblasts differentiation. We carried out immunohistochemistry using cytokeratin to investigate the role of Hertwig's epithelial root sheath and DSPP mRNA in-situ hybridization to conform the nature of root dentin during root development in NFI-C knockout mice. Cytokeratin reacted with all the HERS cells and the continuity of cytokeratin positive cells between the HERS cells and enamel epithelium was lost in the cervical region both wild and K/O types. After root dentin deposition cytokeratin positive-HERS cells showed irregularity and loss of polarity in the cervical region in K/O type. DSPP mRNA was strongly expressed in odontoblasts of crown and root dentin in wild type mice, whereas expression of DSPP mRNA was restricted in odontoblast of crown dentin in the K/O type. During root formation in NFI-C knockout mice, HERS normally grow out of the crown but fail to induce odontoblast differentiation in root portion. These results suggest that NFI-C may play important roles in odontoblast differentiation during root dentin formation.

법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능

박종태,최용석,김흥중,정문진,오현주,신인철,박주철,손호현 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.6

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin,MMP2 mRNA 발현이 감소하였으며, CM-OD314를 transfection하여 OD314의 과발현을 유도한 경우에는 OD314와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다. This study was aimed to elucidate the biological function of OD314 (Apin protein), which is related to ameloblast differentiation and amelogenesis. Apin protein, calcifying epithelial odontogenic (pindborg) tumors (CEOTs)-associated amyloid, were isolated from CEOTs, and has similar nucleotide sequences to OD314. We examined expression of the OD314 mRNA using in-situ hybridization during tooth development in mice. Expression of OD314 and several enamel matrix proteins were examined in the cultured ameloblast cell line up to 28 days by reverse transcription-polymerase chain reaction (RT-PCR) amplification. After inactivation and over-expression of the OD314 gene in ameloblast cell lines using U6 vector-driven RNA interference and CMV-OD314 construct, RT-PCR were performed to evaluate the effect of the OD314 during amelogenesis. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were more strongly expressed in ameloblast than odontoblast. 2. When ameloblast cells were cultured in the differentiation and mineralization medium for 28 days, the tuftelin mRNA expression was maintained from the beginning to day 14, and then gradually decreased to day 28. The expressions of amelogenin and enamelin were gradually decreased according to the ameloblast differentiation. 3. Inactivation of OD314 by U6-OD314 siRNA construct down-regulated the expression of OD314, MMP-20, and tuftelin, whereas over-expression of OD314 by CMV-OD314 construct up-regulated the expression of OD314 and MMP-20 without change in tuftelin. These results suggest that OD314 is considered as an ameloblast-enriched gene and may play the important roles in ameloblast differentiation and mineralization.

혈액 종양환자에서 Tobramycin의 임상약동학

신재국,신완균,장인진,신상구,김성민,배현주,최강원,김진규 대한화학요법학회 1990 대한화학요법학회지 Vol.8 No.1

항암화학요법을 받고 있던 중 감염으로 tobramycin을 투여받은 36명의 혈액종양 환자에서 tobramycin의 약동학적 특성을 비종양환자군에서의 population 값과 비교 검토하였다. 이들은 모두 정상 신기능을 가진 16세 이상의 성인남녀(21:15)백혈병 환자들이었다. 36명의 혈액종양 환자에서 산출된 tobramycin의 청소율 및 체내분포용적은 각각 120.3± 27.2ml/lg/hr 및 0.386± 0.11 L/㎏로 population 추정 치보다 유의하게 큰 값을 보였다.(P. <0.05).청소율과 체내분포용적을 해당 population 추정치로 나눈 비율치(ratio)의 평균값은 각각 1.47± 0.34 및 1.20± 0.34였다. 연령, hematocrit치, 혈청albumin치, 발열 및 항암화학요법기간과 tobramycin의 청소율 및 체내분포용적 사이에 유의한 상관관계는 발견할 수 없었다. 본 연구결과 혈액종양 환자에서 tobramycin 투여시는 적정혈장농도를 유지하기 위해 일반 환자군에 비해 용량의 증가 및 투여간격의 조정이 필요하며 지속적인 혈장농도 monitoring을 통하여 용법의 재적정화가 필요할 것으로 사료된다. The pharmacokinetics of tobramycin were evaluated in 36 hematologic malignancy patients undergoing anticancer chemotherapy and compared to the expected values from the population parameters. Total body clearance(mean : 12.3±27.2㎖/㎏/hr) and volume of distribution (mean : 0.386±0.11 L/㎏) in hematologic malignancy patients with normal renal function were significantly greater than those of estimated from population parameter distribution(P<0.05). The ratios of total body clearance and volume of distribution to the population estimates were 1.44±0.37 and 1.20±.034, respectively. No relationships were found between age, hematocrit, serum albumin, fever or duration of anticancer chemotherapy and pharmacokinetic parameters. It is suggested that the increment of tobramycin dose regimen wold be considered in patients with hematologic malignancy, and dose readjustment followed by close monitoring of plasma drug concentration would be required.