Association of DOCK8, IL17RA, and KLK12 Polymorphisms with Atopic Dermatitis in Koreans

( Won Il Heo ),( Kui Young Park ),( Mi-kyung Lee ),( Yu Jeong Bae ),( Nam Ju Moon ),( Seong Jun Seo ) 대한피부과학회 2020 Annals of Dermatology Vol.32 No.3

Background: Early-onset and severe atopic dermatitis (AD) in patients increase the probability of the development of allergic rhinitis or asthma. Treatment and prevention strategies in infants and young children with AD are targeted toward treating the symptoms, restoring skin barrier functions, and reducing the absorption of environmental allergens in an attempt to attenuate or block the onset of asthma and food allergy. Objective: Given that the initiating events in AD remain poorly understood, identifying those at risk and implementing strategies to prevent AD is necessary. Methods: Whole-exome sequencing (WES) was performed in a 43 control group and a disease group with 20 AD patients without atopic march (AM) and 20 with AM. Sanger sequencing was carried out to validate found variants in cohorts. Results: DOCK8, IL17RA, and KLK12 single-nucleotide polymorphisms were identified by WES as missense mutations: c.1289C>A, p.P97T (rs529208); c.1685C>A, p.P562G (rs12484684); and c.457+27>C, rs3745540, respectively. A case-control study show that total immunoglobulin E (IgE) level was significantly increased in the AA genotype of DOCK8 compared to the CA genotype in allergic patients. The rs12484684 of IL17RA increased risk of adult-onset AD (odds ratio: 1.63) compared to the control for (A) allele frequency. AD and AM Patients with the IL17RA CA genotype also had elevated IgE levels. rs3745540 of KLK12 was associated with AD in dominant model (odds ratio: 2.86). Conclusion: DOCK8 (rs529208), IL17RA (rs12484684), and KLK12 (rs3745540), were identified using a new WES filtering method. the result suggests that polymorphism of DOCK8 and IL17RA might be related to increase the total IgE level. (Ann Dermatol 32(3) 197∼205, 2020)

The Effect of Abdominal Visceral Fat, Circulating Inflammatory Cytokines, and Leptin Levels on Reflux Esophagitis

( Su Youn Nam ),( Il Ju Choi ),( Kum Hei Ryu ),( Bum Joon Park ),( Young Woo Kim ),( Hyun Beom Kim ),( Jeongseon Kim ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.2

Background/Aims Although adipocytes secrete inflammatory cytokines and adipokines, their role in reflux esophagitis is controversial. We investigated the association between visceral fat and inflammatory cytokines or adipokines in reflux esophagitis. Methods Abdominal visceral fat and cytokines were measured in 66 individuals with reflux esophagitis and 66 age- and sex-matched controls. The mean values for visceral fat and cytokines were compared in cases and controls. Second, correlations between visceral fat and inflammatory cytokines were measured. Finally, multiple logistic regression models for odds ratios (ORs) and 95% confidence intervals (CIs) were used to estimate the effects of visceral fat and cytokines on reflux esophagitis. Results Visceral fat, leptin, interleukin (IL)-6, and IL-1β were higher in reflux esophagitis compared to controls. Visceral fat showed a strong positive correlation with IL-6 (r = 0.523, P < 0.001), IL-8 (r = 0.395, P < 0.001), and IL-1β (r = 0.557, P < 0.001), and a negative correlation with adiponectin (r = -0.466, P < 0.001). With adjusted analysis, visceral fat/100 (OR, 4.32; 95% CI, 2.18-8.58; P < 0.001) and leptin (OR, 1.36; 95% CI, 1.10-1.69; P = 0.005) independently increased the risk of reflux esophagitis, but the effects of other cytokines were abolished. Conclusions Visceral fat may increase the risk of reflux esophagitis by increasing the levels of inflammatory cytokines. Leptin showed a positive association with reflux esophagitis that was independent of visceral fat. (J Neurogastroenterol Motil 2015;21:247-254)

Naringin Protects Ovalbumin-induced Asthma through the Down-regulation of MMP-9 Activity and GATA-3 Gene

Chang-Min Lee(이창민),Jeong Hyun Chang(장정현),In Duk Jung(정인덕),Young-Il Jeong(정영일),Noh Kyung Tae(노경태),Hee-ju Park(박희주),Jong-Suk Kim(김종석),Yong Kyoo Shin(신용규),Sung Nam Park(박성남),Yeong-Min Park(박영민) 한국생명과학회 2009 생명과학회지 Vol.19 No.6

Naringin은 레몬, 오렌지에서 발견되는 flavonoid계열에 속하는 물질로 여러 식물과 과일에 다량 함유되어 있다. 항암, 항산화 작용을 하는 것으로 알려져 있는 Naringin을 ovalbumin (OVA)으로 유도한 천식(asthma) 생쥐모델을 이용하여 치료효과를 알아 보았다. 기관지 폐포 세척액을 회수하여 백혈구의 수적 변화, 제2형 협조T세포(Th2 cell)가 생산하는 IL-4, IL-5의 생산에 미치는 영향과 폐조직에서 matrix metalloproteinase (MMP)-9 활성을 측정하였다. 또한, 최근에 Th1/Th2 전사인자로서 GATA-3가 밝혀졌는데 이번 실험에서 Naringin이 ovalbumin (OVA)으로 유도한 천식(asthma) 생쥐모델에서 Th1, Th2 싸이토카인과 유전자 발현을 조절할 수 있는가에 대하여 알아보았다 그 결과 기관지 폐포 세척액에서 OVA로 감작하여 천식을 유도한 실험군에서는 호산구의 현저한 증가, Th2 형 싸이토카인(IL-4, IL-5)의 증가가 관찰되었다. 그러나 Naringin을 투여한 그룹에서는 OVA의 감작에 의하여 증가한 각종 염증성 지표들이 감소하거나 정상화 되었다. 또한 OVA에 의하여 증가된 기도저항성이 Naringin 투여에 의하여 감소하였으며 폐조직의 염증성 소견도 뚜렷하게 감소되었다. 이와 같은 연구 결과는 Naringin이 천식의 치료에 유용하게 쓰일 수 있음을 시사해준다. The common word flavonoids is often used to classify a family of natural compounds, highly abundant in all higher plants, that have received significant therapeutic interest in recent years. Naringin is associated with a reduced risk of heart disease, neurodegenerative disease, cancer and other chronic diseases; however the molecular basis of this effect remains to be elucidated. Thus we attempted to elucidate the anti-allergic effect of Naringin in ovalbumin (OVA)-induced asthma model mice. The OVA-induced mice showed allergic reactions in the airways. These included an increase in the number of eosinophils in bronchoalveolar lavage (BAL) fluid, an increase in inflammatory cell infiltration into the lung around blood vessels and airways, airway luminal narrowing, and the development of airway hyper-responsiveness (AHR). The administration of Naringin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, this study may provide evidence that Naringin plays a critical role in the amelioration of the pathogenetic process of asthma in mice. These findings provide new insight into the immunopharmacological role of Naringin in terms of its effects on asthma in mice.

Electropulsing Treatment on Enhancement of Electrical Conductivity of Screen-Printed Ag Wire

Ju‑Won Park,Howook Choi,Hwangsun Kim,Simoon Sung,Hye‑Jin Jeong,Il Kim,Jaeseok Gong,Sung‑Tae Hong,Heung Nam Han 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5

The effect of high electric current density on the sintering of Ag wires manufactured by screen printing is evaluated throughelectrical resistivity analysis and microstructure observation. Different forms (continuous and pulsed) of electric currentwith different current densities are applied to the specimens. Conventional heat treatment is also performed as a controlgroup to examine the athermal effect of electropulsing treatment. Compared to the conventional heat treatment, the resistivityis reduced more under the electropulsing treatment with continuous current for the same temperature and treatment time.Also, the process time of electropulsing treatment can be reduced by applying a pulse form of high density current insteadof continuous current without losing the benefit of enhanced reduction of resistivity. The microstructural observationsobtained from high angle annular dark field scanning transmission electron microscope and a digital precession instrumentclearly show that necking connecting the crystals is formed more firmly under electric current. In addition, the temperaturechange of Ag wire and substrate is calculated according to the change of the resistivity when the electric current is appliedto confirm the reliability.

항갑상선제로 치료한 그레이브스병 환자의 관해예측인자

남일송,윤여일,김선규,김용현,이주영,목지오,윤석기,김철희,김영선,변동원,서교일,유명희 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background: Most cases of Graves' disease have been treated by antithyroid drugs in Korea. The major drawbacks of antithyroid drugs are the relative high rate of relapse and the inconvinience of long term therapy. Measurement of TBII and TRH stimulation test have been considered as the most reliable tests predicting relapse, but the predictive power is not high enough to use in clinical situation. This study was performed to find good prognostic indicators in Graves' disease patients after the discontinuation of antithyroid drug therapy. Subjects and methods : We restrospectively evaluated 96 patients with Graves' disease who showed normal TRH test at the end of antithyroid drug therapy and were followed for more than one year. Serum T3, T4, T3/T4 ratio, TBII, Anti-TPO Ab and TGAb levels were measured at the time of diagnosis and at the end of therapy. Two to three months after withdrawal of antithyroid medication, serum TSH and free T3 responses to TRH were examined. These parameters were compared between the relapse group and the remission group. Results : Among the 96 patients, 22 patients(22.9%) relapsed(Group Ⅱ) and 74 patients(77.1%) remained in remmision(Group Ⅰ). No significant difference was observed between the relapse and the remission group in clinical parameters, serum T3, T4, TSH, T3/T4 ratio, TBⅡ and Anti-TPO Ab determined before and after treatment. Serum TSH and free T3 levels measured after TRH stimulation were also similar in both groups. Serum TGAb levels determined at the end of treatment were significantly higher in the remission group(p<0.05). Relapse following the discontinuation of therapy occured within 24 months in 72.7% of the relapsed cases. After 24 months, relapse rate was reduced significantly. Conclusion : These results suggest that high serum TGAb levels could be a favorable prognostic indicator for the long term remission of Graves' disease treated with antithyroid drugs, and may suggest that Hashimoto's thyroiditis is combined with Graves' disease in those patients.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

Felodipine 함유 Eudragit microsphere의 생체이용율

김남순,김현주,김영일,양재헌 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Microencapsulations of felodipine which is one of the calcium channal blocker, using the mixture of Eudragit RL®, L® and E® and celullose on the base of Eudragit RS® were investigated. The average diameters of microspheres were from 40 to 55㎛. The incorporation ratios of felodipine into the microspheres were higher than 90 %. After 7h, about 60 % of felodipine was released. Dissolution rate of felodipine from microsperes was increased by the addition of Cremopher®. After oral administration of microspheres, the Cmax were reduced and incresed the bioavailities as up to 50 %.

미역 폐기물의 중금속 흡탈착 특성

조주식,박일남,허종수,이영석 한국환경농학회 2004 한국환경농학회지 Vol.23 No.2

본 연구에서는 생물흡착제인 미역을 사용하여 인공폐액 내의 중금속의 흡착 특성을 조사하였으며, 다음과 같은 결론을 얻었다. 미역 폐기물을 이용하여 제조된 생물흡착제의 단일 중금속 흡착효율은 각 중금속 100 mg/L인 용액에 biosorbent 3 g을 처리했을 때 Pb는 거의 100%였고 Cu와 Cd가 약 85~86% Zn과 Cr은 약 60~64%, Co와 Ni은 약 57%, Mn은 약 48%였다. 복합중금속 흡착효율은 단일중금속 흡착효율에 비하여 Pb를 제외한 다른 중금속들은 크게 감소되었으나 biosorbent g당 전체 중금속 흡착량은 더 증가되었다. 온도, pH에 따른 중금속 흡착효율은 별 차이가 없었으며 전반적으로 pH는 pH 5~6 범위, 온도는 20~30℃ 범위의 실온이 적당한 것으로 나타났다. 생물흡착제의 흡착평형은 모든 중금속이 1시간 이내에 이루어졌다. Freundlich와 Langmuir 등온흡착식을 구하여 실제 폐수처리에 적용여부를 검토한 결과 Pb, Cu, Cr, Cd, Co 순으로 비교적 친화도가 큰 것으로 나타났다. Freundlich 등온흡착식에 가장 잘 일치하였다. 모든 중금속의 Freondlich 지수 1/n값이 0.1~0.5 범위에 포함되어 흡착이 용이하고, Pb, Cr, Cu, Cd 순으로 비교적 평형 흡착량이 큰 것으로 나타났으며, 이 결과는 Langmuir 흡착등온식의 결과와 거의 유사하였다. 따라서, 중금속 농도범위에 따른 한계범위를 설정하여 등온흡착식을 적용시키면 매우 높은 중금속 처리효율을 나타낼 것으로 판단된다. 생물흡착제가 중금속 흡착 후의 구조적인 특성을 조사하기 위하여 FT-IR 분석 결과, 중금속 이온과 치환될 것으로 생각되는 functional group을 확인할 수 있었으며, 3,400/cm에서의 -OH group, 1,648/cm에서의 C=O bond, 1,426/cm에서의 C-O bond 그리고 850/cm에서의 S=O등이 전반적으로 중금속 흡착 후에 peak가 커지는 것이 관찰되었다. 생물흡착제의 재사용 가능성을 검토하기 위하여 최적의 중금속 탈착조건 검토하였는데, 탈착제 종류에 따른 중금속 탈착효율은 탈착제 종류와 중금속 종류에 따라 차이가 있었고, 전반적으로 NTA>H₂SO₄>HCl>EDTA 순으로 우수하였으며, 전체적으로 특정 중금속의 탈착효율이 떨어지지 않는 NTA가 탈착제로 가장 적합하였다. NTA농도에 따른 중금속 탈착효율은 NTA 0.1~0.3% 농도범위에서는 농도 증가에 따라 탈착효율도 약간 증가되었으나 그 이상의 농도에서는 별 차이 없었다. 온도와 pH 변화에 따른 중금속 탈착효율은 Cr을 제외한 다른 중금속들은 크게 영향을 받지 않는 것으로 나타났으며 전반적으로 온도는 30℃, pH는 2에서 높았다. 탈착제 접촉 반응시간에 따른 탈착효율은 10분 이내에 전체 탈착량의 80%이상이 탈착 되었으며 1시간 이후에는 거의 변화가 없었다. The adsorption and desorption of Pb, Cd, Co, Zn, Cr, Co, Ni, and Mo on the waste Undaria sp. were studied. Except for Pb, the mono adsorption rate for all heavy metals were lower than that of the heavy metals mixed. However, the adsorption capacity of the heavy metals by 1 g of biosorbent in mixed heavy metals increased. According to FT-IR analysis of the biosorbent after heavy metal biosorption, the replacement of the functional group by the heavy metals ions could be confirmed and the inverted peaks became larger after heavy metals adsorption. The adsorption equilibrium of heavy metals was reached in about 1 hour. The equilibrium parameters were determined based on Langmuir and Freundlich isotherms. The affinity of metals on the biosorbent decreased in the following order: Pb>Cu>Cr>Cd>Co. The desorption rate decreased in the following sequence: NTA>H₂SO₄>HCl>EDTA. The desorption rate of heavy metals by NTA increased with increase in the concentration from 0.1 to 0.3% but the desorption rate became constant beyond 0.3%. Therefore, it represented that desorption rate of heavy metals was suitable under optimized condition (30℃, pH 2 and 0.3% NTA solution) and was fast with 80% or more the uptake occurring within 10 min of contact time.

一部 美容業 從事者들의 織責別 腰痛 有病率과 關聯要因

김춘일,박종,류소연,김석산,김기순,하상호,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

To find the prevalence and related factors of low back pain experienced by some beauticians. a survey was made toward 252 beauticians in Kwangju City from March 1st to April 1st, 1998. The results were as follows; 1. The prevalence rate of low back pain among the respondents for the last week was 59.5%. 2. The aid group and staff group with low back Pain had significantly longer duration of work than those without low back pain. Managers who had bad posture with twisted or too much extended back showed a significantly higher prevalence of low back pain than those with good posture during work(P<05). 3. Aid group with low back pain showed significantly higher points at total stress score, depression score, general health score and vitality score than those without low back Pain. The staff group with low back pain showed significantly higher points for total stress score, sleep disorder and anxiety score, depression score, general well-being and vitality score than those without low back pain. Managers with low back Pain showed significantly higher points of stress at social performance and self confidence (P<05) than those without low back pain. The above results suggest that low back pain is related to different jobs and work environments for beauticians. So effective measures and further studies against risk factors must be made considering their jobs. To prevent or reduce low back pain, appropriate management of stress for beauticians is necessary.

췌장의 점액성 낭선암 1예

송현주,김도영,정혜경,남승현,문일환,구혜수 梨花女子大學校 醫科大學 醫科學硏究所 2002 EMJ (Ewha medical journal) Vol.25 No.2

Nowadays increasing use of abdominal ultrasound in routine check-up may increase the detection rate asymtomatic cystic lesions of pancreas. Even through the majority of the cystic lesions of pancreas is pseudocyst, about 10-15% of those lesions are caused by pancreatic cystic tumor. In the pancreatic cystic tumor, especially, mucinous cystic tumor should be exicised due to its malignant potential, while the pancreatic pseudocyst or serous cystic tumon can be observed for a period or treated medically. Several clinical, radiological, biochemical and pathologic guidelines have been developed in order to distinguish among them. Among pancreatic cystic tumors, mucinous cystic adenocarcinoma is very rare and accounts for only 1% of all pancreatic neoplasms. Unlike extremely poor prognosis of pancreatic adenocarcinoma, mucinous cystic adenocarcinoma has an indolent course and shows a good prognosis after its curative resection. Recently we experienced a 69-year-old woman who had a mucinous cystic adenocarcinoma of the pancreas. We report this case with a review of literature.