Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

종양병동과 일반병동 간호사의 암성통증 관리에 대한 지식과 수행 비교

이은미(Eun-Mee Lee),박금자(Geum-Ja Park) 고신대학교 전인간호과학연구소 2011 전인간호과학학술지 Vol.5 No.-

Purpose: to compare the level of knowledge and practice regarding cancer pain management between nurses working in general units versus nurses working in oncology units. Method: The subjects of this study were 260 nurses in total. They were 129 nurses in oncology units and 131 nurses in non-oncology units who have had more than 6 months of work experiences recently. Data collection was carried out in six different university hospitals in five different cities in the southern part of Korea The data were collected using structured questionnaires from October 25, 2010 to November 7, 2010. To measure the level of knowledge and practice of cancer pain management in nurses, the instrument, which was initially translated and later revised by Jo, Eun-Kyung(2009), was utilized in this study. The instrument was based on the Cancer Pain Management Guideline (2008) developed by the Korean Ministry for Health, Welfare and Family Affairs. The data were processed and analyzed by SPSS Window 18.0 program in terms of frequency, percentage, mean, t-test, and X 2-test. Results: 1. The results revealed that there was no significant relationship between those two groups when their age, the length and the place of work experiences, religion, education level, work experiences with cancer patiaits and the needs of pain education were compared However, there were significant differences between those two groups when their position, current work place, experience of cancer pain education and the number of exposure to the cancer pain education were compared. 2. Total mean score of knowledge regarding cancer pain management was significantly higher in cancer unit nurses(21.57±4.46) than those(18.78±4.30) in general units (t=-5.130, p< .000). The degree of knovdedge about non-drug therapy (t=-3.834, p< .000) were significantly higher in nurses in oncology units than those in general units. 3. Total mean score of practice regarding cancer pain management was not significantly different in those two grcxips(t=-1.406, p= .161) and the degree of practice about non-drug therapy (t=0.751, p= .453) were significantly higher in oncology unit nurses than those in general units. Conclusion: total mean score of knowledge regarding cancer pain management was significantly higher in cancer unit nurses than those in general units. And total mean score of practice regarding cancer pain management was not significantly different in those two groups. Therefore, it is likely that the education about cancer pain management seems highly recommended for nurses working in non-oncology units as well as nurses in oncology units.

자기 효능의 개념 분석

서순림,이은남,박송자,양영희,이동숙,최은옥,구미옥,김인자,이인숙,김성재,박영임,이은옥 서울대학교 간호대학 간호과학연구소 1997 간호학 논문집 Vol.11 No.1

Today human needs for health promotion and maintenance are increasing. The nursing progression has a great responsibility for helping clients to practice good health behavior. self efficacy was found to be potent predictor in initiating and maintaining the health-related behavior. Therefore, the concept is important in nursig intervention for change of health behavior. The purpose of this paper was to analyze and clarify the meaning of the concept, self efficacy. This study used Walder & Avant's process of concept analysis. Critical attributes of self efficacy were : 1) positive perception of his won capability; 2) intrapersonal strength; 3) possibility of being learned; 4) individuality; 5) specificity; 6) generalizability. Antecedents of self efficacy consist of those facts that 1) new situation occurs; 2) individuals have needs to change the new situation; 3) there are some required actions in new situation. Consequences occurring as a result of self efficacy consist of those facts that 1) individual initiates the of failure; 5) cope with the situation; 6) controls the situation; 7) the level of self efficacy is enhanced; 8) the level of self-esteem is enhanced.

환자, 간호사, 의사가 지각하는 교육요구도와 교육제공정도에 관한 연구

박인숙,문영숙,조미경,최은숙,이경숙,정길수 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

The purpose of this study was to provide the basic data for the planning of education of patients by exploring and comparing patients', nurses' and doctors' perception of the educational needs and degree of providing education. 66 hospitalized patients undergoing chemotherapy and 44 nurses, 42 doctors assigned for the direct care were sampled in 1 university hospital in Taejon from August 30, 1993 to September 30, 1993. For the comparing patients', nurses' and doctors' perecption of the educational needs and degree of providing education, the researcher used Kims' Educational need scale which is composed of 20 item Likert type 5 point scale. Data were analyzed by means of percentile score, SPSS, t-test, ANOVA, Pearson correlation. The result were as follows 1. Comparision of total Mean score patients', nurses' and doctors' perception of the educational need ordering nurses(M=4.0465), patients (M=3.9803), doctors (M=3.8309). The difference between the three group's total Mean score is showed no statistical significant difference. 2. Comparision of total Mean score patients' educational need, patients' percetion of nurses' and doctors' degree of providing education, patients' educational need is scored 3.9803, patients' perception of nurse is scored 2.3431, patients' perception of doctor is scored 2.5022. The difference between the patients' educational need and medical teams' degree of providing education is showed statistical significant difference(P<.001). 3. Comparision of total mean score nurses' educational need is scored 4.0465, nurses' perception of nurses' degree of providing education is scored 2.9920, patients' perception of nurses' degree of providing education is scored 2.3431. The difference of between the nurses' educational need and nurses' perception of nurses' degree of providing education is showed statistical significant difference(P<.001) 4. Comparision of total mean score doctors' educational need is scored 3.8309, doctors' perception of doctors' degree of providing eduation is scored 3.1726, patients' perception of doctors' degree of providing education is scored 2.5022. The difference of between the doctors' educational need and doctors' perception of doctors' degree of providing education is showed statistical difference(P<.001).

흰쥐 재생간의 Monoamine Oxidase 의 활성치

박은미,곽춘식,문교철,김여희 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

This study was intended to investigate the changes of hepatic mitochondrial and microsomal monoamine oxidase(MAO) A and B activities after 70%(median and left lateral lobes) partial hepatectomy in rats. The activities of mitochondrial MAO A and B in the regenerating liver significantly increased between the second and the third days of the operation. However, the microsomal MAO A activity in regenerating liver showed a marked decrease from two days after operation.

한국 부부의 바람(want) 탐색과 위기 극복을 위한 시도 : 부부 관계 증진을 위한 가톨릭 교회의 효율적인 대안 모색을 위하여 Finding the Catholic Church's effective alternatives for developing marital relationships

박은미 가톨릭대학교 인간학연구소 2006 인간연구 Vol.- No.10

In the accelerated materialism, small-sizing of family, changing family value and life styles, the tensions and conflicts of wedding couples might have already been latent. And in the respect that every human is created as a totally different being, a conflict is also a part of human substantial. The most essential thing is how we should take the method to deal with the conflicts. Most of all, it is important to clarify what change each spouse need, and how much he/she has the will to try. If couples develop intimate relationships as a companion, each spouse has to be sensitive to the other spouse's wants. Especially, when unusual of bad atmosphere between spouses begins to rise, couples have to focus to know what they want and they had better learn how to express their wants in order to let their spouse know his/her want. In this thesis, through examining what expects and thoughts ordinary korean wives and husbands have, I would like to find out what tensions and conflict most couples experience, and in which relationships they want to live. For this work, I took a questionnaire method on the subject of marriage couples and unmarried youth. In the Ⅱ part, I will examine the result of the questionnaire, in the Ⅲ part I will suggest that three factors ─ Communication Skill, Conflicts Confronting Skill, Intimacy Developing Activity ─ are very important things for the formation of better marital relationships. In the Ⅳ part, introducing already established couples programs of the Catholic Church, I would like to suggest more effective applying method and the need to make new programs. Each couple has to try to live as a healthy couple. But as the health of couples gives a guarantee to the social health, such trial is not only each couple's problem. The Catholic Church should try to educate the way couples live healthily. Also, for preventing couple's crisis and supporting actually endangered couples, the church has to prepare the appropriate supporting system. For example, the role clarification of each parish's family pastoral department, the application of couple growth programs for the couples of each parish and the establishing family counselling center etc., all of us expect the Catholic Church will make more active supporting structure related couples and family.

백금(Ⅳ)착체의 약리학적 연구

박은희,임경화,신윤진,박미화 숙명여자대학교 약학연구소 1994 약학논문집-숙명여자대학교 Vol.10 No.-

The platinum complex, PtCl₄(2-CEen), was previously synthesized and shown to have anti-tumor activity. In the present study, toxicologic profile of PtCl₄(2-CEen) and its acute anti-inflammatory activity on carrageenin edema was observed with oral administration. The ef-4feet of this agent on acetic acid-induced writhing in mice were also examined. In male and female mice given a single intragastrical dose of PtCl₄(2-CEen), we determined that LD_(50) values were over lOOOmg/kg B.W. In the method of carrageenin edema and writhing test, the PtCl₄(2-CEen), orally administered in a single dose, tended to inhibit the development of hind paw edema in rats, and the number of writhing induced by acetic acid in mice. These facts suggest that PtCl₄(2-CEen) may have weak anti-inflammatory effect.

宜寧 全義李氏 出土服飾의 세척방법에 관한 연구

박윤미,황은경,정복남 服飾文化學會 2003 服飾文化硏究 Vol.11 No.6

The purpose of study is to find out a suitable cleaning method for excavated textiles of Jeon-ui Lee(1570~1647). The tektites were excavated from her tomb in 1997, and her descendents put them in a box without any treatment and kept them in the warehouse since then. We used two kinds of silk as samples, non-dyed and dyed textile. The experiment was performed by 8 kinds of wet cleaning and dry cleaning methods, an ultrasonic cleaner was used in the wet cleaning, decane and perchloroethylene were used as solvents in the dry cleaning. The use of the ultrasonic cleaner in the wet cleaning method did not show any damage to the fibers of the textiles and it not only cleaned well but also was safe for the fugitive dyes. It resulted in more effective cleaning when the detergent was used together. Therefore, it is effective to use the ultrasonic cleaning on the delicate historical textiles and helpful to the operator's safety and environment.

체크리스트를 통한 대학생의 환경실천현황 분석

이은희,정재훈,임미정,박은정 서울여자대학교 학생생활연구소 2011 학생생활연구 Vol.24 No.-

본 연구는 서울여자대학교 대학생의 환경실천현황과 인식조사를 통해 기후변화시대에 대응하기 위한 대학에서의 환경교육 활성화와 실천의 문제점 등을 파악하기 위하여 수행되었다. 연구는 2011학년도 1학기 ‘기후변화와 녹색성장' 과목을 수강한 학생을 대상으로 설문조사를 통해 학생들의 행동양식을 분석하였다. 설문내용은 크게 응답자에 대한 일반사항, 체크리스트에 관한 설문, 그리고 기후변화와 녹색성장과 환경에 대한 사항의 3개 분야로 나누어 41개 문항으로 이루어져 시행되었다. 설문조사를 통해 강의 후 환경의 심각성에 대해 느끼는 정도와 설문대상자들의 환경을 위한 실천 활동 정도 등에 대해 분석한 결과 평소 환경에 관심을 갖고 있던 학생이 50%정도로 나타났으며, 강의 후 환경의 심각성을 느낀다고 답한 학생은 75%정도의 비율을 보여 이는 환경에 대해 큰 관심이 없던 학생들에게 대학에서의 환경교육을 통해 그 심각성을 일깨워준 것으로 나타났다. 또한 수업을 들은 학생의 대부분이 항상 또는 자주, 가끔 등 그 빈도의 차이는 있으나 환경을 위해 평소 생활에서 가능한 실천들을 행하고 있는 것을 확인할 수 있었다. 이러한 결과를 종합해 볼 때 대학에서의 환경교육은 학생들의 환경의식 변화를 꾀하여 보다 지속가능한 삶을 위해 필요한 환경적 인재를 양성하는 것은 물론 지역사회로의 확대를 통해 사회적, 환경적 변화를 가져올 수 있을 것으로 판단된다. 본 연구에서 확인할 수 있었던 학생들의 환경에 대한 인식을 통해 대학에서는 지속가능하며, 로하스적인 삶을 위한 교육을 더욱 확대해야함을 인지하고, 또한 이를 위해 환경에 대한 교육적 프로그램에 관한 연구가 이루어지는 것이 필요하다. 주요어:인식조사, 체크리스트, 한국대학, 환경교육 For this study, the check list for environment were written through the environment education of 'Climate change and green growth', an academic liberal arts subject which is necessary for freshmen of Seoul Women's University. And then, the study was conducted so as to vitalize the environment education of universities for responding the age of climate change based on the survey of students' recognition on environment after the class. For the study, students' behavior pattern was analyzed by the survey for students who attended the subject of ‘Climate change and green growth' during the first semester of 2011. The survey was fulfilled with 41 questionnaires whose contents are divided into 3 fields greatly. Through the survey, the degree that they feel the seriousness of environment, the degree that they conduct environment action, etc. after the class were analyzed. As the result, students who were usually interested in environment were about 50% and students who responded they felt the seriousness of environment after the class were about 75%, so it means that environment education of universities let students who were not interested greatly in environment know its seriousness. Also, most of students who attended the class put the things which are possible in daily life for environment into practice although each student had a difference of the frequency. If synthesizing the above results, environment education of universities is judged to not only cultivate environmental people which are necessary for more sustainable life by promoting the change of students' recognition on environment but also bring social and environmental changes by expanding into regional society. Through the students' recognition on environment, which could be confirmed by this study, universities must recognize that the education for sustainable and LOHAS life should be more expanded and conduct studies on the educational program for environment. Key words:Check List, Environment Education, Korean Universities, Survey of Recognition.

Benzo(a)pyrene에 의한 돌연변이원성과 지질과산화에 미치는 d-Limonene과 Cineole의 억제 효과에 대한 비교연구

강은미,박성배,김상건,정기화 德成女子大學校 藥學硏究所 1993 藥學論文誌 Vol.4 No.1

It has been reported that d-limonene inhibits chemical-induced rat mammary cancer by the mechanism of increases in detoxification enzymes such as glutathione S-transferases and that cineole fails to exhibit significant suppressive effect on chemical-induced carcinogenesis. The present study was designed to compare the effects of d-limonene and cineole on the benzo(a)pyrene (BP)-induced mutagenicity, BP metabolism and lipid peroxidation. Modified Ames assay was employed to evaluate the inhibitory effect of d-limonene and cineole on the BP-induced mutagenicity. The number of revertant-bearing wells was decreased by 44∼77% in the presence of both BP and d-limonene compared with that of BP alone whereas cineole decreased the number of revertant-bearing wells by 28∼45% at the concentrations between 2㎛ and 2mM. d-Limonene suppressed BP metabolism by 16, 54 and 67% at 1, 10 and 100 mM, respectively while cineole inhibited the metabolism by 16, 26 and 55% at the same concentrations. The EC_50 values for d-limonene and cineole in inhibiting lipid peroxidation were 2.0 mM and 16 mM respectively, as assayed by thiobarbituric acid method. The present study showed that d-limonene and cineole have common antimutagenic effects although d-limonene appeared to be more effective than cineole in suppressing mutation and lipid peroxidation. The results suggest that the antimutagenic effects of d-limonene and cineole may be associated with alternation in enzyme activities and with inhibition of lipid peroxidation.