Ehrlich Ascites Carcinima 誘發 생쥐의 生存 其間 延長에 對한 1, 3-bis(2-chloroethy1)-1-Nitrosourea와 Tubercin-3의 倂合效果

金麗姬,鄭泰浩 慶北大學校 醫科大學 1978 慶北醫大雜誌 Vol.19 No.1

흰쥐 간의 Carboxylesterase 활성에 미치는 고부하 Taurocholate의 영향

한병훈,김여희 啓明大學校 醫科大學 1998 계명의대학술지 Vol.17 No.4

목적: 이 연구는 담즙울체 시 간과 혈청에서 활성고가 감소되는 carboxylesterase 활성도 감소기전의 일부를 알아내기 위하여 시행하였다. 대상 및 방법: 흰쥐에게 총담관 결찰로 담관을 폐쇄시킨 직후 또는 총담관 대정맥 문합을 시행한 직후 TCA 또는 TUDCA를 정맥 내에 주입한 다음 경시적으로 간과 혈청에서 carboxylesterase 활성도의 변동에 대한 이들 담즙산의 효과를 측정하였다. 결과: 흰주에게 총담과 대정맥 문합을 한 후 2일 경과했을 때와 담관 폐쇄를 한 후 1일 및 2일 경과시켰을 때 간 mocrosome 분획의 carboxylesterase 활성도와 담관 폐쇄 후 2일경과했을 때 혈청의 carboxylesterase 활성도 등은 통계학적으로 유의한 감소를 나타내었다. 간 microsome 분획 및 혈청의 carboxylesterase 활성도는 총담관 대정맥 문합을 한 군보다 담관 폐쇄를 한 군에서 더 감소되었으며 양군 모두 수술 후 1일 경과했을 때보다 2일 경과했을 때 간과 혈정에서 이 효소의 활성도가 더 감소되었다. 흰주에게 총담관 대정맥 문합 또는 담관 폐쇄를 한 직후 TCA를 주입하여 1일 및 2일 경과했을 때 간 cytosol,mitochondria, 및 microsome 분획의 carboxylesterase의 활성도는 각각의 대조군인 총담관 대정맥 문합만 한 군 또는 담관 폐쇄만 한 rnss에 비해 통계학적으로 유의한 감소를 나타내었다. 흰주에게 총담관 대정맥 문합 또는 담관 페쇄를 한 직후 TCA를 주입하여 1 일 및 2 일 경과했을 때 혈청의 carboxylesterase 활성도는 각각의 대조군인 총담관 대정맥 문합만 한 군 또는 담관폐쇄만 한 군에 비해 유의한 감소를 나타내었다. 흰쥐에게 총담관 대정맥 문합 또는 담관 페쇄를 시행한 직후 TUDCA를 주입하여 1 일 및 2 일 경과했을 때 간 cytosol, mitochondria 및 microsome 분획과 혈청에서 이 효소의 활성도는 모두 대조군과 유의한 차이가 없었다. 흰주에게 총담관 대정맥 문합 또는 담관 폐쇄를 시행하고 2일 경과했을 때 간 microsome 분획의 carboxylesterase의 Vmax치는 가수술만 한 군에 비해 유의한 감소를 나타내었다. 흰주에게 총담관 대정맥 문합 또는 담관 폐쇄를 시행한 직후 TCA를 주입하고 2일 경과했을 때 간 cytosol, mitochondria 및 microsome 분획의 carboxylesterase의 Vmax치는 각각 대조군인 총담관 대정맥 문합만 한 군 또는 담관폐쇄만 한 군에 비해 유의한 감소를 나타내었다. 흰주에게 총담관 대정맥 문합 또는 담관 폐쇄를 시행한 직후 TUDCA를 주입하고 2일 경과했을 때 간 microsome 분획의 carboxylesterase의 Vmax치는 가수술만 한 군에 비해서만 유의한 감소를 나타내었다. 그러나 이 효소의 Km치는 모든 실험군의 간세포 분획에서 유의한 변동의 나타내지 않았다. 요약: 이상의 결과로 보아 담즙울체 간에서 carboxylesterase의 활성도 감소는 담즙산 중 특히 TCA에 의해 이 효소의 합성이 억제되어 나타난 결과로 생각되며 아울러 담즙울체 시 이 효소의 혈청 중 활성도 감소는 간에서 이 효소의 합성 감소로 혈중으로 유출이 감소되어 나타난 결과로 생각된다. Background/Aims: We have investigated the effect of bile acid load on the carboxylesterase activity in cholestatic rat liver and serum in order to elucidate the possible mechanism of decrease of carboxylesterase activity under cholestasis. Methods: Rats were divided into eight groups: Normal, sham operated control, bile duct obstruction (BDO) alone (BDO group), BDO plus taurocholic acid (TCA) injection (BDO plus TCA group), BDO plus tauroursodeoxycholic acid (TUDCA) injection (BDO plus TUDCA group), choledocho-caval shunt (CCS) operation alone (CCS group). CCS plus TCA injection (CCS plus TCA group), and CCS plus TUDCA injection (CCS plus TUDCA group). Carboxylesterase activity was determined in the serum and liver cytosolic, mitochondrial and microsomal preparations from above experimental rats. The values of Km and Vmax in this hepatic enzyme were measured. Results: The activity of liver microsomal carboxylesterase showed a significant decrease in both CCS and BDO group. And the activity of serum carboxylesterase showed a marked decrease in both CCS and BDO group. However, carboxylesterase activity in the serum and liver microsomal preparation fell more rapidly in the BDO group than CCS. Carboxylesterase activity in liver cytosolic, mitochondrial and microsomal preparations, and its Vmax value decreased significantly in both CCS plus TCA group, and BDO plus TCA group than each control group. On the other hand, the values of Km of the hepatic subcellular carboxylesterase did not change in all the experimental group. Serum carboxylesterase activity decreased significantly in both CCs plus TCA group and BDO plus TCA group than each control group. However, these serum and hepatic enzyme activity did not change in both CCS plus TUDCA group and BDO plus TUDCA group. Summary: The above results suggest that TCA repress biosynthesis of the carboxylesterase in the liver. And the lowering of the serum enzyme activity thought to be caused by decrease of biosynthesis of this hepatic enzyme, which cause the enzyme to leak

주정 중독 흰쥐에서 총담관 결찰이 혈청 및 간의 Lactate Dehydrogenase활성에 미치는 영향

곽춘식,안광욱,김여희 啓明大學校 醫科大學 1992 계명의대학술지 Vol.11 No.1

The activities of the liver and serum lactate dehydrogenase were studied when cholestasis was induced by common bile duct(CBD) ligation in rats and chronic ethanol intoxication developed, or cholestasis following acute ethanol intoxication for manifestation of the biochemical background of alcohol intoxication in hepatobiliary disease. There was no significant change in the liver and serum lactate dehydrogenase activities of rats treated with both acute or chronic ethanol intoxition. The liver lactate dehygrogenase activity showed a significant decrease at the 2nd , 3rd, 7th and 14th days follwing the ligation in both the group with the ligation follwing the chronical ethanol intoxication and the groups only with CBD ligation. The acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats liver lactate dehydrogenase activities decreased considerably and the same seen in the group with the 14th day following the CBD ligation. The groups that received CBD ligation after being chronically intoxicated with ethanol showed a marked increade at the 1st, 2nd, 3rd, 7th and 14th days floolwing the ligaion in the sera lactate dehydrogenase activities. However, the activities showed a far higher degree than groups of the CBD ligation. For the groups of acute intoxication with ethanol done after 14 days of the CBD ligation, the sera lactate dehydrogenase activities increased markadly, but the activities showed a higher degree than the group with the 14th day following the CBD ligation. According to the above results, when the acute and chronic ethanol intoxication with cholestasis occurred, the sera lactate dehydrogenase activities are higher than in cholestasis because of increaded liver cell damage. which causes the enzyme to leak into the blood in great quantity. According, these results will be the data supprorting alcoholic drink is enzymologically harmful in hepatobiliary disease.

주정 중독 흰쥐에서 총담관 결찰이 간의 β-D-Glucuronidase활성에 미치는 영향

곽춘식,임종술,김여희 啓明大學校 醫科大學 1992 계명의대학술지 Vol.11 No.3

The activities of the liver and serum β-D-glucuronidase were studied when cholestasis was induced by common bile duct ligation in rats and chronic ethanol intoxication developed, or cholestasis following acute ethanol intoxication for manifestation of the biochemical background of alcohol intoxication in hepatobiliary disease. When common bile duct was ligated in the rats, lysosomal and microsomal β-D-glucuronidase activities in the cholestatic liver and activity of β-D-glucuronidase in the serum showed a considerable increase. When common bile duct was ligated after chronic ethanol intoxication, the activities of liver lysosomal β-D-glucuronidase decreased more significantly than in the group with common bile duct ligation, while the activities of liver microsomal β-D-glucuronidase and serum β-D-glucuronidase increased more significantly than in the group with common bile duct ligation performed. The activities of liver lysosomal and microsomal β-D-glucuronidase and serum β-D-glucuronidase in the group with 14th day after common bile duct ligation increased markedly, and the same was seen in tile group with acute ethanol intoxication after common bile duct ligation. At the 24th hour following the acute intoxication with ethanol done after 140days of the common bile duct ligation, the rats showed more remarkable increase in the serum β-D-glucuronidase activity than the group only with the 14th day after the common bile duct ligation. In summary, the liver lysosomal β-D-glucuronidase seem to be an enzyme which decreases activities in chronic ethanol intoxication with cholestasis more than in cholestasis. And the liver microsomal β-D-glucuronidase seem to be an enzyme which increases activities in chronic ethanol intoxication with cholestasis more than in cholestasis. The causes of the increase or the decrease seems to be the development or the retardmrnt of biosynthesis. Especially, when the acute and chronic ethanol intoxication with cholestasis occurred, the sera β-D-glucuronidase are higher than in cholestasis because of increased liver cell damage, which causes the enzyme to leak into the blood in great quantity. Accordingly, these results will be the data supporting alcoholic drink is enzymologically harmful in hepatobiliary disease.

주정 중독 흰쥐에서 총담관결찰이 간 및 혈청의 Malate Dehydrogenase 활성에 미치는 영향

곽춘식,안광욱,임종술,김여희 啓明大學校 醫科大學 1991 계명의대학술지 Vol.10 No.3

The activities of the liver and serum malate dehydrogenase(MDH) were studied when cholestasis was induced by common bile duct ligation and chronic ethonic intoxication developed, or cholestasis following acute ethanol intoxiaction in hepatobiliary disease. The rats liver cytosolic MDH activities showed a significant increase in both chronically ethanol intoxicated groups and acutely ethanol intoxicated groups. But the rats liver mitochondrial MDH activities showed no significant changes in both chronically ethanol intoxiacted groups and acutely ethanol intoxicated groups. The groups that received common bile duct(CBD) ligation after being chronically intoxicated with ethanol showed a considerable decrease at the 2nd, 3rd, 7th and 14th days following the ligation in the liver cytosolic MDH activities. However, the activities showed a higher degree than groups of the CBD ligation. On the other hand, the liver mitochondrial MDH activities showed a significant decrease at the 3rd, 7th and 14th days following the ligation, but the activities showed a higher degree on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours following the acute intoxication with ethanol done after 14days of the CBD ligation, the rats showed a slight decrease in the liver cytosolic and mitochondrial MDH activities than the goup only with the acute ethanol intoxication. However, the activities showed more remarkable increase than the goup sacrificed on the 14th day following the CBD ligation. The groups that receved CBD ligation after being chronically intoxicated with ethanol showed a dramatic increase at the 1st, 2nd, 3rd, 7th and 14th days following the ligation in the sera MDH activities. However, the activities showed a far higher degree than groups of the CBD ligation. For the goups of acute intoxication with ethanol done after 14 days of the CBD ligation, the sera MDH activities increased markedly, but the activities showed a higher degree than the goup with the 14th day following the CBD ligation. According to the above results, the liver cytosolic and mitochondrial MDH seems to be an enzyme which increases activities in both acute and chronic ethanol intoxication with cholestasis more than in cholestasis; the cause of the increase seems to be the development of biosynthesis. Especially, when the acuted and chronic ethanol intoxication with cholestsis occurred, the sera MDH are higher than in chlestasis because of increased liver cell damage, which causes the enzyme to leak into the blood in great quantity. Accordingly, these results will be the data supporting that alcoholic drink is enzymologically harmful in hepatobiliary disease.

흰쥐 재생간의 Leucine Aminopeptidase의 활성치

문교철,곽춘식,김여희 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

Changes in the activities of the followings have been studied over a period of 6 days after partial hepatectomy in rats: Plasma membrane, mitochondrial, nuclear and cytosolic leucine aminopeptidase and microsomal-particle bound aminopeptidase of the regenerating liver and serum leucine aminopeptidase. The activities of alkaliine phosphatase in the subcellular fractions were also measured. The activities of leucine aminopeptidase and alkaline phosphatase in serum markedly elevated from 12 hours to three days after partial hepatectomy in rats. The activity of plasma memberane-bound leucine aminopeptidase in the regenerating rat liver drastically increased during the first and the third days of the operation. And the activity of microsomal particle-bound aminopeptidase in the regenerating liver significantly increased between the second and the third days after operation. The activities of nuclear and cytosolic leucine aminopeptidase in the regenerating liver showed a substantial increase at the second day and from first to sixth day respectively after operation. However, no significant change in hepatic mitochondrial leucine aminopeptidase was noted throughout the experiments. The activities of plasma membrane, microsomal, mitochondrial, nuclear and cytosolic alkaline phosphatase in the regenerating liver markedly increased throughout the experiments.

만성 주정 중독 흰쥐에서 총담관 결찰이 UDP-Glucuronosyltransferase 활성에 미치는 영향

조경일,김여희 啓明大學校 醫科大學 1999 계명의대학술지 Vol.18 No.3

The alcoholic hazard in hepatobiliary disease was studied by the assays of liver microsomal UDP-glucuronosyltransferase activity in cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication in rats. The microsomal UDP-glucuronosyltransferase activity and the Vmax value of the enzyme in the cholestatic rat liver combined with chronic ethanol intoxication showed further significant decrease compared to the activity from cholestasis alone. However, the Km value of th enzyme did not change. The results indicate that the biosynthesis of the liver UDP-glucuronosyltransferase is decreasing in chronic ethanol intoxication with cholestasis than in cholestasis alone.

Ethanol중독 흰쥐에서 총담관결찰이 간의 Glutathione S-Transferase, Glutathione Peroxidase 및 Glutathione Reductase 활성에 미치는 영향

곽춘식,박은미,문교철,김여희 啓明大學校 醫科大學 1989 계명의대학술지 Vol.8 No.2

This study was made to see the effect of common bile duct ligation on liver glutathione S-transferase (GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities in rats suffereing from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5%(v/v) ethanol instead of water for 60 days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were then killed on the lst, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic, mitochondrial and microsomal GST, and cytosolic GSH-Px activities of the liver, The liver GR activities were also measured. For acute iintoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5th and 24th hours for mesuring the activities of the above enzyme. The rats liver cytosolic and microsomal GST activities showed slight increase in chromically ethanol intoxicated group but the mitochondrial GST activities did not increase in thies group. The rats liver cytosolic, mitochondrial and microsomal GST activities showed no significatn changes in acutely ethanol intoxicated groups. In terms of rats liver GR and liver cytosolic GSH-Px activities, no significant changes were shown in either chronically ethanol intoxicated groups or actely ethanol intoxicated groups. The groups that received common bile duct (CBD) ligation after being chronically intoxicated with ethanol showed considerable decrease in the liver cytosolic GST activities. However, the activities showed a less degree than groups of CBD ligation. The liver mitochondrial GST activities of the CBD ligation groups showed slight decrease at the 14th day of the ligatioin. But the activities of the groups with the ligation after chronic ethanol intoxication showed of the ligation. But the activities of the groups with the ligation after chronic ethanol intoxicatioin showed significant decrease at the 2nd, 3rd, 7th and 14th days followiing the operation. The liver microsomal GST activities of the (CBD) ligation groups showed remarkable increase at the 7th and 14th days of the ligation. But the activities showed no significant increase in the groups with the ligation following the chronic ethanol intoxication. The groups that received CBD ligation after being chronically intoxicated with ethanol showed considerale inicrease at the 2nd, 3rd, 7th and 14th days following the operation in the liver GR activiites. On the other hand, the liver cytosolic GSH-Px activities showed significant increase at the 14th days after the ligation. However, the activities showed a far less on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours followiing the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed less remarkable decrease in the liver cytosolic GST and GSH-Px activities than the group only with the 14th day following CBD ligatioin. The liver microsomal GST liver GR activites, howvere, showed considerable increase at the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation. But the activities showed a less degree than group with the 14th day following CBD ligatioin. At the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the liver mitochondrial GST activity decreased significantly, and the same was seen in the group with the 14th day following CBD ligatioin.

흰쥐 재생간의 Monoamine Oxidase 의 활성치

박은미,곽춘식,문교철,김여희 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

This study was intended to investigate the changes of hepatic mitochondrial and microsomal monoamine oxidase(MAO) A and B activities after 70%(median and left lateral lobes) partial hepatectomy in rats. The activities of mitochondrial MAO A and B in the regenerating liver significantly increased between the second and the third days of the operation. However, the microsomal MAO A activity in regenerating liver showed a marked decrease from two days after operation.

흰쥐 재생간의 알콜대사효소들의 활성치

문교철,곽춘식,정성광,김여희 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

A study was made on the change in the activities of the following during 10 post-operative days: alcohol dehydrogenase of regenerating rat livers after 70% (median and left lateral lobes) partial hepatectomy. After partial hepatectomy in the rats, activities of regenerating liver alcohol dehydrogenase significantly increased in the sixth day. The activities of microsomal ethanol-oxidizing system in the regenerating liver markedly increased from the third day after partial hepatectomy. However, no significant changes in hepatic catalase was noted throughout the experiments. The activities of cytosolic and microsomal aldehyde dehydrognease in the regenerating liver significantly increased from the sixthe day and the third to the sixth day respectively, but mitochondrial aldehyde dehydrogenase showed no change.