유산균(L.lactis CBT-8)및 발효 추출물의 Helicobacter pylori 억제 능력

진춘조,박형석,이혜운,김성렬,강동구,이준상,이재동,왕준호 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Helicobacter pylori infection was the cause of chronic gastritis, duodenal ulcer and gastric MALT lymphoma. The eradication rates of H. pylori using antibiotics are around 80%. Lactobacilli have been demonstrated to have in vivo and in vitro inhibitory effects on H. pylori infection. We aimed to evaluate the inhibitory effect of L. lactis CBT-8 and its extract on H. pylori in human stomach. Thirty-five H. pylori-infected volunteers(30-49 yr)were randomized into two groups which were treated with L. lactis CBT-8 and its extract(Group A n=17) or milk containing L. lactis CBT-8(Group B, n=18). They underwent 13C-urea breath tests before and after treatment. The DOB30 of group A(n=14) was decreased 41.0% after treatment(26.0±4.9 before, 15.3±2.1 after treatment)(p<0.05). the DOB30 of group B(n=13) was decreased 22.3% after treatment(22.0±2.2 before, 17.1±1.7 after treatment)(p<0.05). In conclusion, L. lactis CBT-8 was effective in suppressing H. pylori infection in human stomach.

약물 유해반응 보고지침 -임상시험의 안전성에 관한 정보 관리지침 -

지동현,김훈교,배진우,손동렬,김인범,전용관,박혜연 大韓臨床藥理學會 1998 臨床藥理學會誌 Vol.6 No.2

일측 자골신경의 절단 및 재연결 후 흰쥐 척수에서 신경세포 접착분자(NCAM)의 면역조직반응의 변화에 관한 형태학적 연구

황세진,전복관,안동춘,조원길,김승현 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

하이데거의 존재론적 차이에 대해

全東震 水原大學校 1993 論文集 Vol.11 No.-

Die "ontologische Differenz", d.h. die Differenz von Sein und Seiendem nennt die Sache des Denkens, auf die Heideggers Denken standig unterwegs war. Der spate Heidegger faβt ontologische Differenz als den "Unter-Schied" von Lichtung und Anwesenheit auf. Dieser Unter-Schied ist ein Grundverhaltnis in dem je konkert ereignenden Seinsgeschehen, das der spate Heidegger als "Ereignis" zu denken versucht. Diese Grundstruktur des Ereignisses kommt aber schon in der "fundamentalontologischen" Frage nach dem "Sinn von Sein uberhaupt" als das Verhaltnis von Sein (des Innerweltlichen) und Zeit (der Welt) zur Sprache. So wird in diesem Aufsatz zunachst die Intention der Seinsfrage geklart und dann das Verhaltnis von Lichtung und Anwesen. Auβerdem wird gezeigt, daβ die Metaphysik ihre "onto-theologische". Wesensverfassung gerade der Verstellung des Unter-Schiedes von Lichtung und Anwesenheit verdankt.

道와 存在 : 노자와 하이데거의 근본사상 비교

全東震 水原大學校 1997 論文集 Vol.15 No.-

Dieser Aufsatz erortert die gedankliche Nahe und Ferne zwischen Heidegger und Lao-tzu. Das namenlose 'Tao' des Lao-tzu entspricht nicht Heideggers 'Sein' bzw. 'Ereignis'. Von Heidegger her gesehen ist es ein metaphysisches Wort. Aber Lao-tzus Konzeption von der Weghaftigkeit als Grundcharakter allen Ph anomens und dem 'Nicht-Tun' als der Weise der ursprunglichen Antwort des Menschen zu dem in sich bewegten Phanomen zeigt eine Verwandtschaft mit Heideggers Konzeption des weghaften Seins und der 'Gelassenheit'.

2-구획 인공 모세혈관 모델을 이용한 Pseudomonas aeruginosa 임상균주에 대한 Panipenem의 효과

이동건,전혜선,최수미,최정현,유진홍,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.4

목적 : Panipenem은 일본에서 개발된 카바페넴계 항생제로 광범위한 항균력을 나타낸다고 보고되고 있지만 P. aeruginosa에 대해서는 아직 논란이 있다. 본 연구에서는 임상에서 분리된 P. aeruginosa 균주를 대상으로 사람의 약역학을 모의할 수 있는 시험관내 역동 모델을 이용하여 panipenem (PAPM)과 imipenem (IMPM)의 효과를 비교하고 Muller-Hinton broth (MHB)와 basic amino acid 함량이 적은 배지(Minimum Broth Davis, MBD)에 효과의 차이가 있는지 확인하고자 하였다. 재료 및 방법 : 혈액에서 분리된 P. aeruginosa SGP14 균주를 대상으로 하였다. 사람의 약동학을 모의할 수 있는 2-구획 인공 모세혈관 모델(two-compartment model)을 제작하였고, 세균이 peripheral compartment에서 반감기에 따라 농도가 변하고 있는 항생제에 노출되고, 접종한 세균의 수는 희석되지 않게 하였다. PAPM과 IMPM의 용량은 500mg씩 하루 2회를 모의하도록 하였고 2일간 실험하였다. Panipenem, imipenem의 반감기는 사람의 약역학과 같이 10시간에 맞추었다. 항생제 투여 간격은 panipenem, imipenem 모두 12시간으로 하였고 각 항생제의 최고 및 최저농도는 사람에 투여했을 때의 혈중농도에 맞추었다(panipenem 각각 45.6, 0 ug/mL, imipenem 각각 33.0, 0 ug/mL). 배지는 MHB와 MBD를 사용하였고 4군으로 나누어 실험하였다. [PAPM (MHB), PAPM (MBD), IMPM (MHB), IMPM (MBD)] peripheral compartment에서 0, 1, 2, 4, 6, 8, 12, 24, 32, 48시간에 맞춰 샘플을 하였고 집락수 (cfu/mL)를 측정하였다. 결과 : SGP14에 대한 PMPM, IMPM의 MIC는 각각 64, 2 ug/mL이었다. PAPM (MHB)는 2시간이 지나면서 다시 자라기 시작하여 32시간째부터는 항생제를 사용하지 않은 대조군과 같은 곡선을 보였다. 12시간까지의 집락수 변화는 4가지 실험군에서 군간 의미있는 차이를 보이지 않았지만(P=0073) 군내의 PAPM (MHB)가 PAPM(MBD), IMPM (MBD)와 비교하여 집락수 변화가 유의하게 작았다. 또한 48시간까지의 집락수 변화는 PAPM(MBD)이 가장 많았고, 그 다음이 IMPM (MHB), IMPM(MBD)였으며, PAPM(MHB) 순이었다(P=000). 48시간까지 집락수 변화는 IMPM (MHB)와 IMPM (MBD)사이에 차이가 없었다. 결론 : 사람의 혈청내 basic amino acid 농도와 PAPM의 농도를 모의하도록 한 2-구획 인공 모세혈관 모델에서 PAPM은 IMPM과 비교하여 P. aeruginosa에 대해 비슷하거나(12시간째) 더 나은 살균력을 보였다(48시간째). 추후 본 실험의 결과와 실제 임상에서의 P. aeruginosa에 대한 panipenem의 효과를 비교해보는 것이 필요하겠다. Background : Panipenem (PAPM) is a new carbapenem which has an enhanced broad spectrum activity against both gram-positive and negative organisms. However, its activities in vitro against Pseudomonas aeruginosa are still under controversy. The aim of this study was to compare the activity of PAPM with those of imipenem (IMPM) against clinical isolates of P. aeruginosa using in vitro kinetic model and to evaluate the differences according to the quantity of basic amino acid in media. Materials and methods : Using a clinical isolate of P. aeruginosa (SGP14) from blood, an in vitro 2-compartment artificial capillary model based on a dialyzer unit was prepared. Antibiotics were given as a bolus q12 hrs for 48 hrs. Simulated doses and frequencies of PAPM and IMPM were 500 mg q12 hrs as approved by Korea Food and Drug Administration. Muller-Hinton broth (MHB) and minimal broth Davis (MBD) were used as culture media and we divided the experiments into 4 groups [PAPM (MHB), PAPM (MBD), IMPM (MHB), IMPM (MBD)]. At 0, 1, 2, 4, 6, 8, 12, 24, 32, and 48 h, samples were removed from peripheral compartment and viable bacterial counts were measured. Results : The susceptibility of PAPM and IMPM for SGP14 were 64 and 2 ug/mL in MHB and 4 and 2 ug/mL in MBD, respectively. Up until 12 hours, changes in bacterial colony counts were not significantly different (P=0.073) for each group. However among the four groups, PAPM (MHB) showed the least changes compared with PMPM (MBD), IMPM (MBD). The largest decrement of colony during 48 hours was observed with PMPM (MBD), followed by IMPM (MHB) or IMPM (MBD), and PAPM (MHB) in decreasing order (P=0.00). There were no differences between IMPM (MHB) and IMPM (MBD) as for the change in colony counts. Conclusions : The bactericidal activities of panipenem against the clinical isolate of P. aeruginosa was similar (at 12 h) or superior (at 48 h) to that of imipenem in an in vitro 2-compartment artificial capillary model using minimal broth to simulate human serum drug concentrations.

혈액질환 환자에서 조혈모세포 이식과 화학요법 후의 침습성 진균감염의 분석

유진홍,최정현,이동건,최수미,신완식,김춘추 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

목적 : 조혈모세포 이식이나 화학요법을 받은 혈액질환 환자에서 침습성 진균감염의 양상을 분석하고 예후 인자를 정하기 위하여 이 연구를 실시하였다. 방법 : 2000년 3월부터 2001년 2월까지 가톨릭 조혈모세포이식센터에 입원한 318명의 환자들 중 진균 감염으로 의심되어 항진균제를 투여받은 122명의 환자를 대상으로 후향적 분석을 실시하였다. 결과 : 74명의 환자가 EORTC/MSG 기준에 의해 침습성 진균증으로 판정되었다. 이들 중 6명이 확진이었고, 46명이 추정, 22명이 가능 증례이었다. 가장 많이 침습된 장기는 하부 호흡기이었다(72/74). 사망률은 32.4%이었다. 단변량 분석으로 예후 인자를 도출한 결과 입원 기간, 진단까지 걸린 시일, 그리고 amphotericin B의 총 투여량이 유의한 인자로 나왔다. 그러나 다변량 분석을 시행한 결과 입원 기간만이 독립적으로 유의하게 영향을 미치는 예후 인자로 산출되었다. 결론 : 침습성 진균 감염은 현재도 조혈모세포 이식이나 화학요법 후의 중요한 합병증으로 작용하고 있음을 알 수 있었다. 진균 감염 합병증의 예후에 영향을 미치는 요소로 입원 기간이외에 진단의 신속성과 항진균제의 투여도 유의한지에 대해서는 후속 연구를 통해 재치 규명해야 할 것으로 생각된다. Background : Invasive fungal infections (IFI) are one type of representing total infections in compromised patients. To analyze the characteristics and to determine the prognostic factors of IFI in patients with hematologic diseases undergoing hematopoietic stem cell transplantation (HSCT) and/or chemotherapy. Methods : We retrospectively reviewed consecutive patients (n=122) who received antifungal treatment with the impression of fungal infection complicating post-HSCT or chemotherapy from March-2000 to February-2001. Results : Seventy-four out of 122 registered patients belonged to the IFI on the basis of EORTC/MSG criteria. Six patients were proven IFI, 46 were probable, and 22 were in the category of possible one. The most commonly involved organ was lower respiratory tract (72/74). The mortality was 32.4%. Univariate analysis revealed duration of hospitalization, days to diagnosis of IFI, and total amount of amphotericin B as significant prognostic factors. But multivariate analysis determined only duration of hospitalization from these variables as an independently influencing factor on the prognosis. Conclusion : Invasive fungal infection is still the major threatening complication of HSCT and chemotherapy. Further follow-up and extension of this study is necessary to elucidate more prognostic factors.

후두결핵의 임상적 고찰

이동건,심우영,조재민,박현민,이진춘,왕수건 釜算慶南耳鼻咽喉科學會 1996 임상이비인후과 Vol.7 No.2

환경교육을 위한 웹 기반 프로젝트 학습 시스템의 설계 및 구현

전우천,정효숙,김동용,엄종진,정성모,최수경 서울교육대학교 2001 한국초등교육 Vol.12 No.2

The use of the Web (World Wide Web) has been changing roles of traditional education style. Especially, the Web can overcome time and space limitations in the traditional schools. Teachers and students are now using the Web to access vast amounts of information and resources on cyber space. Also, the learning via the Web enables both synchronous and asynchronous communication. Despite of many benefits of the Web, it may weaken students motivation due to lack of face-to-face communication. In this paper, we provide a learning model called Web Project Learning, which is based on the contructivism, to provide motivation and collaborative learning for students in the Web environment. The model is based on the Project-Based Learning model and is revised for use in the Web. The model can also encourage the participation of parents as well as students, and be applied to any subject. We implement our model and show that the model can be used for environmental education as an instance.

비흡연자의 Nicotine에 대한 노출량과 뇨중 Cotinine 농도의 상관성에 관한 연구

노진호,신동천,김종만,정용 한국환경독성학회 1990 환경독성보건학회지 Vol.5 No.2

Smoking damages nonsmoker's health who have been exposed to passive smoking as well as smoker's own health. Passive smoking can cause serious health damage to particular groups, such as the old aged, children and pregnant women. The purpose of this study is to investigate the relationship between nicotine concentrations in environmental tobacco smoke (ETS) and urinary cotinine concentrations of nonsmokers exposed to ETS, and to provide basic information related to health risk assessment. The results of this study were summarized as follows: 1. When 180 cigarrette were smoked during 5 hours (high concentrations exposure) in 132 m³ chamber, mean concentrations of nicotine in ETS showed 263.52㎍/m³±51.93. When 45 cigarretts were smoked (low concentrations exposure), it was 69.43±8.96㎍/m³. 2. The urinary cotinine concentrations of each times (0, 2.5, 5, 17 and 24 hours) in nonsmokers ranged from 0.27~12.52ng/ml in high concentrations exposure and 0.22~2.28ng/ml in low concentrations exposure. Mean while the total urinary cotinine concentrations during 24 hours ranged from 11.62~31.65㎍/ml in high concentrations exposure and 3.45~5.64ng/ml in low concentrations exposure. 3. The correlation equation and coefficient between cotinine concentrations in nonsmokers' urine(y) and nicotine concentrations in ETS(x) was y=0.421+0.0171x and 0:875 (p<0.01) respectively. 4. The quantity of nonsmokers' smoking exposure by passive smoking can be assumed as based on the estimation of nicotine concentrations in ETS by measuring cotinine concentrations of nonsmokers' urine.