칼라 히스토그램과 엔트로피를 이용한 동영상 장면전환 검출

송현석,안강식,안명석,조석제 한국해양대학교 산업기술연구소 2002 연구논문집 Vol.19 No.-

In content-base video data retrieval, the detection for scene change is needed. Color histogram approaches are generally used. Color histogram approaches are simple and more robust to object and camera movements but they ignore the spatial information and fail when two different frames have similar histograms. We propose a new algorithm to use color histogram and entropy to enhance the false-positive occurred by brightness variation and the miss by similar color histogram. At the experiments, it is more robust to brightness variation and have spatial information.

국내 상수원수의 유기물 특성에 따른 소독부산물 생성능

염철민,변석종,조순행,윤제용 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

This study was conducted to investigate the characteristics of disinfection by-products formation potential (DBPFPs) of dissolved organics. Dissolved organics in raw water were separated into two fractions ((1) hydrophilic and (2) hydrophobic) for the measurement of DBPs. Three types of DBPs (trihalomethanes (THMs), haloacetic acids (HAAs), and total organic halides (TOX)) were measured at 3days and 7days incubation time. Sample waters were collected from the Han River and the Wonchun Reservoir. Two major findings are as follows. First, THMFPs/DOC, HAAFPs/DOC and TOXFPs/DOC in the hydrophilic organic component had a level of lower value than those of the hydrophobic organic component. Brominated THMs and bominated HAAs were not found in the chlorinated hydrophobic organic component. Second, the magnitude of HAAFPs/DOC were higher than that of THMFPs/DOC in both hydrophilic and hydrophobic organic components. This results support the reported observation that HAAs formations were higher than that of THMs in chlorinated drinking water. THMFPs and HAAFPs comprised 53-91% of TOXFP.

평균내부거리를 이용한 데이터 분류에 관한 연구

유현재,김문수,조석제 韓國海洋大學校電波通信硏究所 1999 電波通信硏究所論文集 Vol.1 No.-

This paper presents an approach which classifys more accurately clusters for the data sets being different size cluster. We have more degree of membership to the large cluster and less degree of membership to the small cluster by the size of cluster. In the proposed algorithm, the internal data in the average intracluster distance was given the degree of membership more than 1. So we assume the data is proper. FCM is given the degree of membership depends on the distance between data and the center of cluster. But in the proposed algorithm, the center searching was improved about different size cluster by giving the degree of membership using the distance from each data to intracluster.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

국내 주요 상수원수와 처리수에서 HAAs 생성 특성

염철민,최유식,변석종,조순행,윤제용 대한상하수도학회 2002 상하수도학회지 Vol.16 No.2

This study was conducted to investigate the haloacetic acid (HAAs) formation characteristics in raw and treated water in Korea. The raw and treated water from the five major rivers were examined to measure the HAAs concentrations and compared those with literature values. Major findings are as follows. First, average HAAs concentration of treated water (collected from three water treatment plants) in Korea appeared to be 62.5±66.7㎍/L (n=7) and this level is higher than the average trihalomethanes (THMs) concentration (13.2±12.9㎍/L, n=6933) reported recently. Second, the average formation potential of TCAA and DCAA after three days was found as 103±74㎍/L (n=14) and 76±36㎍/L (n=14), respectively. As this result was applied to US-EPA developed model equation for TCAA and DCAA formation, dissolved organic matter (DOM) in Korea has a tendency to produce more TCAA and DCAA as compared to US. This phenomenon was especially notable in DCAA. The sum of weight percentage of TCAA and DCAA which are individual component of HAAS was composed of 80% of HAAs.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

The Beneficial Effect of Chronic Graft-versus-Host Disease on the Clinical Outcome of Transplantation with Fludarabine/Busulfan-Based Reduced-Intensity Conditioning for Patients with De Novo Myelodysplastic Syndrome

Cho, Byung-Sik,Kim, Yoo-Jin,Cho, Seok-Goo,Kim, Sung-Yong,Eom, Ki-Seong,Kim, Hee-Je,Lee, Seok,Min, Chang-Ki,Kim, Dong-Wook,Lee, Jong-Wook,Min, Woo-Sung,Kim, Chun-Choo Springer-Verlag 2007 INTERNATIONAL JOURNAL OF HEMATOLOGY Vol.85 No.5

<P>Allogeneic hematopoietic stem cell transplantation following reduced-intensity stem cell transplantation (RIST) has enabled the treatment of older or medically infirm patients with myeloid malignancies; however, determining the value of RIST outcomes for myelodysplastic syndrome (MDS) is difficult because of the heterogeneity of the diseases included in most trials. To define the role of RIST in MDS, we performed RIST for 22 consecutive patients who had de novo MDS as classified by World Health Organization (WHO) criteria and who received an allograft with fludarabine/busulfan (Busulfex) or fludarabine/Busulfex/antithymocyte globulin (ATG) conditioning. Nineteen patients (86.4%) achieved engraftment. At a median follow-up of 18.9 months (range, 13.1-24.8 months), the estimated 2-year rates of overall survival, event-free survival (EFS), transplantation-related mortality, and relapse were 78.7%, 67.7%, 12.6%, and 22.5%, respectively. Acute graft-versus-host disease (GVHD) greater than grade II developed in 3 patients (15.8%). Chronic GVHD developed in 10 patients (55.6%), none of whom received ATG as a conditioning regimen. Variables influencing EFS were chronic GVHD, marrow blasts before transplantation, and the WHO criteria. The present study clarifies the benefits of the fludarabine/Busulfex-based conditioning regimen for de novo MDS diagnosed according to the WHO criteria and shows that chronic GVHD appears to have a beneficial effect on survival rates, which are strongly associated with graft-versus-tumor effects.</P>

Degradation of Lowland Forest Landscape and Management Strategy to Improve Ecological Quality in Mt. Baekja and Its Surroundings

Cho, Hyun-Je,Cho, Yong-Chan,Lee, Chang-Seok The Ecological Society of Korea 2006 Journal of Ecology and Environment Vol.29 No.5

The number of species and forest area has decreased as urbanization is progressed. The landscape degradation was examined by analyzing vegetation map, satellite image and characteristics of actual vegetation. The study was conducted in Mt. Baekja and its surroundings located on Gyeongsan city, southeastern Korea. As the result of landscape analysis, agricultural field was a characteristic attribute of the study area. Lowlands of this study area were occupied by agricultural field and various plantations. For 15 years from 1987 to 2002, forest area decreased from 2,072.9 ha to 1,853.2 ha, and shape index and fractal dimension of vegetation patches increased from 1.32 to 1.65 and from 1.05 to 1.09, respectively. Pinus densiflora Siebold & Zucco. community showed the highest species diversity, whereas Larix kaempferi (Lamb.) Carriere community showed the lowest species abundance. As forest management implications, monitoring of endangered plant species (Jeffersonia dubia (Maxim.) Benth. & Hook.f. ex Baker & S.Moore), and restoration of lowland forest from plantation to natural forest were discussed. Further, establishment of greenways utilizing existing streams, roadside, and public facilities were recommended.

Induction of REDD1 <i>via</i> AP-1 prevents oxidative stress-mediated injury in hepatocytes

Cho, Sam Seok,Kim, Kyu Min,Yang, Ji Hye,Kim, Ji Young,Park, Su Jung,Kim, Seung Jung,Kim, Jae Kwang,Cho, Il Je,Ki, Sung Hwan Elsevier 2018 FREE RADICAL BIOLOGY AND MEDICINE Vol.124 No.-

<P><B>Abstract</B></P> <P>Regulated in development and DNA damage responses 1 (REDD1) is an inducible gene in response to various stresses, which functions as a negative regulator of the mammalian target of rapamycin protein kinase in complex 1. In the present study, we identified the role of REDD1 under the oxidative stress-mediated hepatocyte injury and its regulatory mechanism. REDD1 protein was increased in H<SUB>2</SUB>O<SUB>2</SUB> or <I>tert</I>-butylhydroperoxide (t-BHP)-treated hepatocytes<SUB>·</SUB> H<SUB>2</SUB>O<SUB>2</SUB> also elevated REDD1 mRNA levels. This event was inhibited by antioxidants such as diphenyleneiodonium chloride, <I>N</I>-acetyl-<SMALL>L</SMALL>-cysteine, or butylated hydroxy anisole. Interestingly, we found that H<SUB>2</SUB>O<SUB>2</SUB>-mediated REDD1 induction was transcriptionally regulated by activator protein-1 (AP-1), and that overexpression of c-Jun increased REDD1 protein levels and REDD1 promoter-driven luciferase activity. Deletion of the putative AP-1 binding site in proximal region of the human <I>REDD1</I> promoter significantly abolished REDD1 transactivation by c-Jun. A NF-E2-related factor 2 activator, <I>tert</I>-butylhydroquinone treatment also elevated REDD1 levels, but it was independent on NF-E2-related factor 2 activation. Furthermore, we observed that REDD1 overexpression attenuated H<SUB>2</SUB>O<SUB>2</SUB> or t-BHP-derived reactive oxygen species formation as well as cytotoxicity. Conversely, siRNA against REDD1 aggravated t-BHP-induced reactive oxygen species generation and cell death. In addition, we showed that REDD1 was induced by <I>in vitro</I> or <I>in vivo</I> ischemia/reperfusion model. Our results demonstrate that REDD1 induction by oxidative stress is mainly transcriptionally regulated by AP-1, and protects oxidative stress-mediated hepatocyte injury. These findings suggest REDD1 as a novel molecule that reduced susceptibility to oxidant-induced liver injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> REDD1 was enhanced in response to oxidative stress. </LI> <LI> AP-1 site from − 644 to − 638 bp in human <I>REDD1</I> was critical for gene expression. </LI> <LI> REDD1 protects cells against ROS in hepatocytes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Construction of Stably Transformed Bm 5 Cells by Using Autographa californica Nuclear Polyhedrosis Virus IE 0 Gene

Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2

To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells